Rabenosyn 5 (RBSN) (NM_022340) Human Tagged ORF Clone

CAT#: RG212488

ZFYVE20 (GFP-tagged) - Human zinc finger, FYVE domain containing 20 (ZFYVE20)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RBSN
• Synonyms: Rabenosyn-5; ZFYVE20
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212488 representing NM_022340
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCTCTGGACGACCCAGGGGAAGTGAGGGAGGGCTTCCTCTGCCCTCTGTGCCTGAAGGATCTGC
  AGTCTTTCTATCAGCTTCACTCACATTACGAGGAAGAACACTCAGGGGAAGACCGTGATGTCAAAGGGCA
  AATTAAAAGTCTTGTCCAGAAGGCTAAAAAAGCAAAGGACAGGTTGTTGAAACGAGAAGGGGATGATCGA
  GCAGAGTCAGGGACCCAAGGATATGAGTCTTTCAGCTATGGAGGGGTTGATCCTTACATGTGGGAACCCC
  AGGAGCTTGGTGCTGTGAGAAGCCATCTTTCCGACTTCAAAAAACACCGAGCTGCTAGAATTGACCACTA
  TGTTGTGGAAGTCAATAAACTAATAATCAGGTTAGAGAAGCTCACTGCATTTGACAGAACAAATACTGAG
  TCTGCAAAGATTCGAGCAATAGAAAAGTCTGTGGTGCCTTGGGTCAACGACCAGGATGTCCCTTTCTGTC
  CAGACTGTGGGAATAAGTTCAGCATCCGGAACCGCCGCCACCACTGCCGCCTCTGCGGGTCTATTATGTG
  CAAGAAGTGTATGGAGCTCATCAGCCTTCCCTTGGCAAACAAGCTCACCAGTGCCAGCAAGGAGTCCCTG
  AGCACCCACACCAGCCCCAGCCAGTCACCCAACAGTGTCCATGGCTCCCGCCGAGGCAGCATCAGCAGCA
  TGAGCAGTGTCAGCTCGGTCCTGGATGAGAAGGACGATGACCGGATCCGCTGCTGTACACACTGCAAGGA
  CACGCTGCTCAAGAGAGAGCAGCAGATTGATGAGAAGGAGCACACACCTGACATCGTGAAGCTCTACGAG
  AAATTACGACTTTGCATGGAGAAAGTTGACCAGAAAGCTCCAGAATACATCAGGATGGCAGCATCATTAA
  ATGCTGGGGAGACAACCTACAGTCTGGAACATGCCAGTGACCTTCGAGTGGAAGTGCAGAAAGTGTATGA
  GCTGATAGACGCTTTAAGTAAGAAGATCTTAACCTTGGGCTTGAACCAGGACCCTCCACCACATCCAAGC
  AATTTGCGGCTGCAGAGAATGATCAGATACTCAGCTACACTTTTTGTGCAGGAAAAGTTGCTTGGTTTGA
  TGTCACTGCCAACCAAAGAACAGTTTGAGGAACTGAAAAAGAAAAGGAAGGAGGAAATGGAGAGGAAGAG
  GGCCGTGGAGAGACAAGCTGCCCTGGAGTCCCAGCGAAGGCTTGAGGAAAGGCAGAGTGGCCTGGCTTCT
  CGAGCGGCCAACGGGGAGGTGGCATCTCTCCGCAGGGGCCCTGCCCCCTTGAGAAAGGCTGAGGGCTGGC
  TCCCACTGTCAGGAGGTCAGGGGCAGAGTGAGGACTCAGACCCGCTCCTCCAGCAGATCCACAACATCAC
  ATCATTCATCAGGCAGGCCAAGGCCGCGGGCCGCATGGATGAAGTGCGCACTCTGCAGGAGAACCTGCGG
  CAGCTGCAGGACGAGTATGACCAGCAGCAGACAGAGAAGGCCATCGAGCTGTCCCGGAGGCAGGCTGAGG
  AGGAGGACCTGCAGCGGGAACAGCTGCAGATGTTGCGTGAACGGGAGTTGGAACGAGAAAGGGAGCAGTT
  TCGGGTGGCATCCCTGCACACACGGACTCGGTCCCTGGACTTCAGAGAAATCGGCCCTTTTCAGCTGGAG
  CCCAGCAGAGAGCCTCGCACCCACCTTGCTTATGCTTTGGATCTAGGCTCTTCCCCAGTTCCAAGCAGCA
  CAGCTCCCAAGACCCCTTCACTTAGCTCAACTCAACCCACCAGAGTGTGGTCTGGGCCCCCAGCCGTTGG
  CCAGGAGCGCTTACCCCAGAGCAGCATGCCACAGCAACATGAGGGGCCCTCCTTAAACCCCTTTGATGAG
  GAAGACCTCTCCAGCCCCATGGAAGAGGCCACTACTGGTCCTCCTGCTGCAGGGGTTTCCTTAGACCCTT
  CAGCCCGCATCCTGAAAGAGTACAATCCTTTCGAGGAAGAGGACGAGGAGGAGGAAGCAGTGGCAGGGAA
  TCCATTCATTCAGCCAGACAGCCCAGCTCCTAACCCCTTCAGTGAGGAAGACGAACATCCCCAGCAGAGG
  CTCTCAAGCCCTCTGGTTCCTGGTAACCCCTTTGAGGAACCCACCTGTATCAACCCCTTTGAGATGGACA
  GTGACAGTGGGCCAGAGGCTGAGGAGCCCATAGAGGAAGAGCTCCTCCTGCAGCAGATCGATAACATCAA
  GGCATACATCTTTGATGCCAAGCAGTGCGGCCGCCTGGATGAGGTAGAGGTGCTGACAGAGAATCTGCGG
  GAGCTGAAGCACACCCTGGCCAAGCAGAAGGGGGGCACTGACTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212488 representing NM_022340
  Red=Cloning site Green=Tags(s)
  
  MASLDDPGEVREGFLCPLCLKDLQSFYQLHSHYEEEHSGEDRDVKGQIKSLVQKAKKAKDRLLKREGDDR
  AESGTQGYESFSYGGVDPYMWEPQELGAVRSHLSDFKKHRAARIDHYVVEVNKLIIRLEKLTAFDRTNTE
  SAKIRAIEKSVVPWVNDQDVPFCPDCGNKFSIRNRRHHCRLCGSIMCKKCMELISLPLANKLTSASKESL
  STHTSPSQSPNSVHGSRRGSISSMSSVSSVLDEKDDDRIRCCTHCKDTLLKREQQIDEKEHTPDIVKLYE
  KLRLCMEKVDQKAPEYIRMAASLNAGETTYSLEHASDLRVEVQKVYELIDALSKKILTLGLNQDPPPHPS
  NLRLQRMIRYSATLFVQEKLLGLMSLPTKEQFEELKKKRKEEMERKRAVERQAALESQRRLEERQSGLAS
  RAANGEVASLRRGPAPLRKAEGWLPLSGGQGQSEDSDPLLQQIHNITSFIRQAKAAGRMDEVRTLQENLR
  QLQDEYDQQQTEKAIELSRRQAEEEDLQREQLQMLRERELEREREQFRVASLHTRTRSLDFREIGPFQLE
  PSREPRTHLAYALDLGSSPVPSSTAPKTPSLSSTQPTRVWSGPPAVGQERLPQSSMPQQHEGPSLNPFDE
  EDLSSPMEEATTGPPAAGVSLDPSARILKEYNPFEEEDEEEEAVAGNPFIQPDSPAPNPFSEEDEHPQQR
  LSSPLVPGNPFEEPTCINPFEMDSDSGPEAEEPIEEELLLQQIDNIKAYIFDAKQCGRLDEVEVLTENLR
  ELKHTLAKQKGGTD
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022340
• ORF Size: 2354 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_022340.2, NP_071735.2
• RefSeq Size: 6678
• RefSeq ORF: 2355
• Locus ID: 64145
• Domains: FYVE
• Protein Families: Transcription Factors
• Protein Pathways: Endocytosis
• Gene Summary: This gene encodes a protein that belongs to the FYVE zinc finger family of proteins. The encoded protein interacts with Ras-related proteins that regulate membrane trafficking. A missense mutation in this gene is associated with a defect in the early endocytic pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]