alpha 1,2 Mannosidase IA (MAN1A1) (NM_005907) Human Tagged ORF Clone

CAT#: RG213002

MAN1A1 (GFP-tagged) - Human mannosidase, alpha, class 1A, member 1 (MAN1A1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MAN1A1
• Synonyms: HUMM3; HUMM9; MAN9
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG213002 representing NM_005907
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCGTGGGGGGCCTGTTGCCGCTCTTCAGCAGCCCCGCGGGCGGCGTCCTGGGCGGGGGGCTCGGCG
  GCGGCGGTGGCAGGAAGGGGTCGGGCCCCGCCGCCCTCCGCCTGACGGAGAAGTTCGTGCTGCTGCTGGT
  ATTCAGCGCCTTCATCACGCTCTGCTTCGGGGCGATCTTCTTCCTGCCAGACTCCTCCAAGCTGCTCAGC
  GGGGTCCTGTTCCACTCCAGCCCCGCCTTGCAGCCGGCCGCCGACCACAAGCCCGGGCCCGGGGCGCGCG
  CCGAGGACGCGGCCGAGGGGCGAGCCCGGCGCCGCGAGGAGGGGGCACCCGGGGACCCGGAGGCCGCCCT
  GGAGGACAACTTGGCCAGGATCCGCGAAAACCACGAGCGGGCTCTCAGGGAAGCCAAGGAGACCCTGCAG
  AAGCTGCCCGAGGAGATCCAAAGAGACATCCTACTGGAGAAGAAGAAGGTGGCCCAGGACCAGCTGCGTG
  ACAAGGCGCCGTTCAGAGGCCTGCCCCCGGTGGACTTCGTGCCCCCAATCGGGGTGGAGAGCCGGGAGCC
  CGCCGACGCCGCCATCCGCGAGAAAAGGGCAAAGATCAAAGAGATGATGAAACATGCTTGGAATAATTAT
  AAAGGTTATGCCTGGGGATTAAATGAACTCAAACCTATATCAAAAGGAGGCCATTCAAGCAGTTTGTTTG
  GTAACATCAAAGGAGCAACTATAGTAGATGCCCTGGATACACTTTTTATTATGGAAATGAAACATGAATT
  TGAAGAAGCAAAATCATGGGTTGAAGAAAATTTAGATTTTAATGTGAATGCTGAAATTTCTGTCTTTGAA
  GTAAATATACGCTTTGTTGGTGGACTACTCTCAGCCTACTATCTGTCTGGAGAAGAGATTTTTCGAAAGA
  AAGCAGTGGAACTTGGGGTAAAATTGCTACCTGCATTTCATACTCCCTCTGGAATACCTTGGGCATTGCT
  GAATATGAAAAGTGGTATTGGAAGGAACTGGCCCTGGGCCTCTGGAGGCAGCAGTATTCTGGCAGAATTT
  GGAACCCTGCATTTGGAGTTTATGCACTTGAGCCACTTATCAGGAAACCCCATCTTTGCTGAAAAGGTAA
  TGAATATTCGAACAGTACTGAACAAACTGGAAAAACCACAAGGCCTTTATCCTAACTATCTGAATCCCAG
  TAGTGGACAGTGGGGTCAACATCATGTATCAGTTGGAGGACTTGGAGACAGCTTCTATGAGTATTTGCTG
  AAGGCCTGGTTAATGTCTGACAAGACAGATCTGGAAGCTAAGAAGATGTATTTTGATGCTGTTCAGGCTA
  TCGAGACTCATTTGATCCGCAAGTCTAGCAGCGGACTAACTTATATCGCAGAGTGGAAAGGGGGCCTCCT
  GGAGCACAAGATGGGCCACCTGACCTGCTTCGCGGGGGGCATGTTCGCACTCGGGGCTGATGCAGCTCCC
  GAAGGCATGGCCCAACACTACCTTGAACTCGGGGCTGAAATTGCCCGTACTTGTCATGAATCATATAATC
  GAACATTTATGAAACTGGGACCAGAAGCTTTCAGATTTGATGGTGGTGTTGAAGCCATCGCTACAAGACA
  AAATGAAAAATACTACATCTTACGGCCAGAAGTTATGGAGACTTACATGTATATGTGGAGACTGACTCAT
  GATCCAAAGTACAGGAAATGGGCCTGGGAAGCCGTAGAGGCCTTGGAAAACCATTGCAGAGTGAATGGAG
  GCTATTCAGGCCTAAGGGATGTTTACCTTCTTCATGAGAGTTATGATGATGTGCAGCAGAGTTTCTTCCT
  GGCAGAGACATTGAAATATTTGTACCTAATATTTTCTGACGACGATCTTCTTCCACTGGAGCATTGGATC
  TTCAATAGCGAGGCACATCTTCTCCCTATCCTCCCTAAAGATAAAAAGGAAGTTGAAATCAGAGAGGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG213002 representing NM_005907
  Red=Cloning site Green=Tags(s)
  
  MPVGGLLPLFSSPAGGVLGGGLGGGGGRKGSGPAALRLTEKFVLLLVFSAFITLCFGAIFFLPDSSKLLS
  GVLFHSSPALQPAADHKPGPGARAEDAAEGRARRREEGAPGDPEAALEDNLARIRENHERALREAKETLQ
  KLPEEIQRDILLEKKKVAQDQLRDKAPFRGLPPVDFVPPIGVESREPADAAIREKRAKIKEMMKHAWNNY
  KGYAWGLNELKPISKGGHSSSLFGNIKGATIVDALDTLFIMEMKHEFEEAKSWVEENLDFNVNAEISVFE
  VNIRFVGGLLSAYYLSGEEIFRKKAVELGVKLLPAFHTPSGIPWALLNMKSGIGRNWPWASGGSSILAEF
  GTLHLEFMHLSHLSGNPIFAEKVMNIRTVLNKLEKPQGLYPNYLNPSSGQWGQHHVSVGGLGDSFYEYLL
  KAWLMSDKTDLEAKKMYFDAVQAIETHLIRKSSSGLTYIAEWKGGLLEHKMGHLTCFAGGMFALGADAAP
  EGMAQHYLELGAEIARTCHESYNRTFMKLGPEAFRFDGGVEAIATRQNEKYYILRPEVMETYMYMWRLTH
  DPKYRKWAWEAVEALENHCRVNGGYSGLRDVYLLHESYDDVQQSFFLAETLKYLYLIFSDDDLLPLEHWI
  FNSEAHLLPILPKDKKEVEIREE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005907
• ORF Size: 1959 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005907.2, NP_005898.2
• RefSeq Size: 4139 bp
• RefSeq ORF: 1962 bp
• Locus ID: 4121
• Cytogenetics: 6q22.31
• Domains: Glyco_hydro_47
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• Gene Summary: 'This gene encodes a class I mammalian Golgi 1,2-mannosidase which is a type II transmembrane protein. This protein catalyzes the hydrolysis of three terminal mannose residues from peptide-bound Man(9)-GlcNAc(2) oligosaccharides and belongs to family 47 of glycosyl hydrolases. [provided by RefSeq, Jul 2012]'