alpha 1,2 Mannosidase IA (MAN1A1) (NM_005907) Human Untagged Clone

CAT#: SC314336

MAN1A1 (untagged)-Human mannosidase, alpha, class 1A, member 1 (MAN1A1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAN1A1
• Synonyms: HUMM3; HUMM9; MAN9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005907, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCCGTGGGGGGCCTGTTGCCGCTCTTCAGCAGCCCCGCGGGCGGCGTCCTGGGCGGGGGGCTCGGCG
  GCGGCGGTGGCAGGAAGGGGTCGGGCCCCGCCGCCCTCCGCCTGACGGAGAAGTTCGTGCTGCTGCTGGT
  ATTCAGCGCCTTCATCACGCTCTGCTTCGGGGCGATCTTCTTCCTGCCAGACTCCTCCAAGCTGCTCAGC
  GGGGTCCTGTTCCACTCCAGCCCCGCCTTGCAGCCGGCCGCCGACCACAAGCCCGGGCCCGGGGCGCGCG
  CCGAGGACGCGGCCGAGGGGCGAGCCCGGCGCCGCGAGGAGGGGGCACCCGGGGACCCGGAGGCCGCCCT
  GGAGGACAACTTGGCCAGGATCCGCGAAAACCACGAGCGGGCTCTCAGGGAAGCCAAGGAGACCCTGCAG
  AAGCTGCCCGAGGAGATCCAAAGAGACATCCTACTGGAGAAGAAGAAGGTGGCCCAGGACCAGCTGCGTG
  ACAAGGCGCCGTTCAGAGGCCTGCCCCCGGTGGACTTCGTGCCCCCAATCGGGGTGGAGAGCCGGGAGCC
  CGCCGACGCCGCCATCCGCGAGAAAAGGGCAAAGATCAAAGAGATGATGAAACATGCTTGGAATAATTAT
  AAAGGTTATGCCTGGGGATTAAATGAACTCAAACCTATATCAAAAGGAGGCCATTCAAGCAGTTTGTTTG
  GTAACATCAAAGGAGCAACTATAGTAGATGCCCTGGATACACTTTTTATTATGGAAATGAAACATGAATT
  TGAAGAAGCAAAATCATGGGTTGAAGAAAATTTAGATTTTAATGTGAATGCTGAAATTTCTGTCTTTGAA
  GTAAATATACGCTTTGTTGGTGGACTACTCTCAGCCTACTATCTGTCTGGAGAAGAGATTTTTCGAAAGA
  AAGCAGTGGAACTTGGGGTAAAATTGCTACCTGCATTTCATACTCCCTCTGGAATACCTTGGGCATTGCT
  GAATATGAAAAGTGGTATTGGAAGGAACTGGCCCTGGGCCTCTGGAGGCAGCAGTATTCTGGCAGAATTT
  GGAACCCTGCATTTGGAGTTTATGCACTTGAGCCACTTATCAGGAAACCCCATCTTTGCTGAAAAGGTAA
  TGAATATTCGAACAGTACTGAACAAACTGGAAAAACCACAAGGCCTTTATCCTAACTATCTGAATCCCAG
  TAGTGGACAGTGGGGTCAACATCATGTATCAGTTGGAGGACTTGGAGACAGCTTCTATGAGTATTTGCTG
  AAGGCCTGGTTAATGTCTGACAAGACAGATCTGGAAGCTAAGAAGATGTATTTTGATGCTGTTCAGGCTA
  TCGAGACTCATTTGATCCGCAAGTCTAGCAGCGGACTAACTTATATCGCAGAGTGGAAAGGGGGCCTCCT
  GGAGCACAAGATGGGCCACCTGACCTGCTTCGCGGGGGGCATGTTCGCACTCGGGGCTGATGCAGCTCCC
  GAAGGCATGGCCCAACACTACCTTGAACTCGGGGCTGAAATTGCCCGTACTTGTCATGAATCATATAATC
  GAACATTTATGAAACTGGGACCAGAAGCTTTCAGATTTGATGGTGGTGTTGAAGCCATCGCTACAAGACA
  AAATGAAAAATACTACATCTTACGGCCAGAAGTTATGGAGACTTACATGTATATGTGGAGACTGACTCAT
  GATCCAAAGTACAGGAAATGGGCCTGGGAAGCCGTAGAGGCCTTGGAAAACCATTGCAGAGTGAATGGAG
  GCTATTCAGGCCTAAGGGATGTTTACCTTCTTCATGAGAGTTATGATGATGTGCAGCAGAGTTTCTTCCT
  GGCAGAGACATTGAAATATTTGTACCTAATATTTTCTGACGACGATCTTCTTCCACTGGAGCATTGGATC
  TTCAATAGCGAGGCACATCTTCTCCCTATCCTCCCTAAAGATAAAAAGGAAGTTGAAATCAGAGAGGAAT
  AA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_005907
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005907.3, NP_005898.2
• RefSeq Size: 5044 bp
• RefSeq ORF: 1962 bp
• Locus ID: 4121
• Cytogenetics: 6q22.31
• Domains: Glyco_hydro_47
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• Gene Summary: 'This gene encodes a class I mammalian Golgi 1,2-mannosidase which is a type II transmembrane protein. This protein catalyzes the hydrolysis of three terminal mannose residues from peptide-bound Man(9)-GlcNAc(2) oligosaccharides and belongs to family 47 of glycosyl hydrolases. [provided by RefSeq, Jul 2012]'