Dystrobrevin alpha (DTNA) (NM_032980) Human Tagged ORF Clone

CAT#: RG213549

DTNA (GFP-tagged) - Human dystrobrevin, alpha (DTNA), transcript variant 6

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DTNA
• Synonyms: D18S892E; DRP3; DTN; DTN-A; LVNC1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG213549 representing NM_032980
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCCAGATCAGCCTGAGAAGCCACTCAACTTGGCTCACATCGTGCCTCCCAGACCTGTAACCAGCA
  TGAACGACACCCTGTTCTCCCACTCTGTTCCCTCCTCAGGAAGTCCTTTTATTACCAGGAGCTCTCCTCC
  CAAGGACAGTGAAGTAGAGCAGAACAAACTGCTGGCTAGGGCTGCTCCAGCTTTTCTGAAGGGCAAAGGC
  ATGCTTGAGAGTTCAAACCGGCTTGATGAAGAACACAGGCTAATTGCCAGGTATGCGGCAAGGCTGGCAG
  CAGAGTCCTCTTCGTCTCAGCCACCTCAGCAGAGAAGTGCTCCTGACATCTCTTTCACCATCGATGCGAA
  TAAGCAGCAAAGGCAGCTGATTGCTGAGCTAGAAAACAAGAACAGAGAAATCTTACAGGAGATCCAGAGA
  CTTCGGCTAGAGCATGAACAAGCTTCTCAGCCCACGCCAGAGAAGGCACAGCAAAACCCCACCCTGCTGG
  CAGAACTCCGGCTCCTCAGACAGCGCAAAGATGAGCTGGAACAGAGAATGTCTGCTCTCCAGGAGAGCCG
  GAGAGAGCTAATGGTCCAGTTGGAGGGTCTCATGAAGCTACTAAAGACTCAGGGGGCAGGCTCTCCCCGC
  TCCTCCCCCAGCCACACCATCAGCAGGCCAATTCCCATGCCCATCCGGTCAGCGTCAGCCTGCTCCACCC
  CGACGCACACGCCGCAGGACTCCCTCACAGGAGTAGGGGGAGATGTACAAGAGGCATTTGCACAAAGTTC
  AAGAAGAAACTTAAGGAATGACTTGCTAGTGGCTGCAGATTCCATCACTAACACTATGTCCTCTCTTGTG
  AAAGAGCTGAATTCTGAGGTTGGGAGTGAAACAGAGAGTAATGTGGATTCTGAATTTGCACGGACTCAGT
  TTGAGGATCTTGTTCCCTCACCAACCTCTGAAAAGGCTTTTCTAGCGCAAATCCATGCCCGAAAACCTGG
  GTACATTCACAGTGGAGCTACCACAAGTACCATGCGTGGCGACATGGTTACGGAGGATGCAGATCCCTAT
  GTGCAGCCTGAAGATGAAAACTATGAAAATGACTCTGTCCGGCAGCTGGAGAATGAGCTCCAGATGGAGG
  AATACCTGAAACAGAAGCTGCAAGATGAAGCTTATCAGGTCAGCTTGCAAGGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG213549 representing NM_032980
  Red=Cloning site Green=Tags(s)
  
  MFPDQPEKPLNLAHIVPPRPVTSMNDTLFSHSVPSSGSPFITRSSPPKDSEVEQNKLLARAAPAFLKGKG
  MLESSNRLDEEHRLIARYAARLAAESSSSQPPQQRSAPDISFTIDANKQQRQLIAELENKNREILQEIQR
  LRLEHEQASQPTPEKAQQNPTLLAELRLLRQRKDELEQRMSALQESRRELMVQLEGLMKLLKTQGAGSPR
  SSPSHTISRPIPMPIRSASACSTPTHTPQDSLTGVGGDVQEAFAQSSRRNLRNDLLVAADSITNTMSSLV
  KELNSEVGSETESNVDSEFARTQFEDLVPSPTSEKAFLAQIHARKPGYIHSGATTSTMRGDMVTEDADPY
  VQPEDENYENDSVRQLENELQMEEYLKQKLQDEAYQVSLQG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032980
• ORF Size: 1173 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032980.2, NP_116762.2
• RefSeq Size: 5221 bp
• RefSeq ORF: 1176 bp
• Locus ID: 1837
• Cytogenetics: 18q12.1
• Protein Families: Druggable Genome
• Gene Summary: 'The protein encoded by this gene belongs to the dystrobrevin subfamily of the dystrophin family. This protein is a component of the dystrophin-associated protein complex (DPC), which consists of dystrophin and several integral and peripheral membrane proteins, including dystroglycans, sarcoglycans, syntrophins and alpha- and beta-dystrobrevin. The DPC localizes to the sarcolemma and its disruption is associated with various forms of muscular dystrophy. Mutations in this gene are associated with left ventricular noncompaction with congenital heart defects. Multiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]'