ATP2B3 (NM_001001344) Human Tagged ORF Clone

CAT#: RG213737

ATP2B3 (GFP-tagged) - Human ATPase, Ca++ transporting, plasma membrane 3 (ATP2B3), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ATP2B3
• Synonyms: CFAP39; CLA2; OPCA; PMCA3; PMCA3a; SCAX1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG213737 representing NM_001001344
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGACATGGCCAATAGTTCCATCGAGTTCCACCCCAAGCCCCAGCAGCAGCGGGATGTCCCCCAGG
  CTGGAGGCTTTGGGTGCACGCTGGCGGAGCTGCGCACCCTCATGGAGCTGCGAGGGGCCGAGGCGCTGCA
  GAAGATCGAGGAGGCCTACGGGGATGTCAGCGGGCTCTGCCGGAGGCTGAAGACCTCACCCACAGAGGGC
  CTGGCGGACAACACCAATGACCTGGAGAAGCGCAGGCAGATCTACGGGCAGAACTTCATCCCCCCAAAGC
  AACCCAAGACCTTCCTGCAGCTGGTGTGGGAGGCCCTGCAGGACGTGACCCTCATCATCCTGGAGGTGGC
  TGCCATCGTCTCTCTGGGCCTCTCGTTCTATGCGCCGCCAGGAGAGGAGAGTGAAGCCTGTGGGAATGTG
  TCGGGAGGCGCAGAAGATGAGGGCGAGGCCGAAGCTGGCTGGATCGAGGGGGCTGCCATCCTGCTGTCCG
  TCATCTGTGTGGTGCTGGTCACGGCCTTCAATGACTGGAGCAAGGAGAAGCAGTTCCGAGGCCTGCAGAG
  CCGAATTGAGCAGGAGCAGAAGTTCACGGTCATCCGGAACGGGCAGCTCCTCCAGGTCCCCGTGGCTGCG
  CTGGTGGTGGGGGACATTGCCCAGGTCAAGTACGGCGACCTGCTGCCAGCCGACGGCGTGCTCATCCAGG
  CCAATGACCTCAAGATCGACGAGAGCTCCCTGACGGGCGAGTCTGACCACGTGCGCAAGTCAGCTGACAA
  AGATCCCATGCTGCTCTCAGGCACTCATGTCATGGAAGGTTCTGGAAGAATGGTGGTGACCGCCGTTGGC
  GTGAATTCCCAGACAGGCATCATCTTCACGCTGCTTGGAGCTGGCGGAGAGGAGGAAGAGAAGAAAGATA
  AGAAAGGCAAGCAGCAGGATGGGGCCATGGAGAGTAGCCAGACCAAAGCTAAGAAGCAGGATGGTGCAGT
  GGCCATGGAGATGCAGCCCCTGAAGAGCGCGGAGGGTGGGGAGATGGAGGAGCGGGAGAAGAAGAAAGCC
  AACGCACCCAAAAAGGAGAAGTCTGTCCTTCAGGGGAAGCTCACAAAGCTAGCCGTGCAGATCGGGAAAG
  CAGGGCTGGTGATGTCTGCCATCACCGTCATCATCCTGGTCCTCTACTTTGTGATTGAGACGTTTGTCGT
  GGAAGGCCGGACATGGCTGGCAGAGTGCACGCCGGTCTATGTACAATACTTCGTGAAGTTCTTCATCATT
  GGTGTCACTGTGCTGGTCGTGGCTGTCCCAGAGGGCCTGCCTCTTGCTGTCACCATCTCCTTAGCTTACT
  CTGTCAAGAAAATGATGAAAGACAACAACCTGGTGCGCCACCTGGATGCCTGCGAGACCATGGGCAACGC
  CACAGCCATCTGCTCCGACAAGACGGGCACGCTCACCACCAACCGTATGACCGTGGTCCAGTCCTACCTA
  GGGGACACCCACTACAAAGAGATTCCGGCCCCCAGCGCCCTGACCCCTAAGATCCTCGACCTCCTGGTCC
  ATGCCATCTCCATCAACAGTGCCTATACCACCAAAATACTACCTCCTGAGAAGGAAGGCGCCCTCCCACG
  CCAGGTGGGCAATAAGACGGAGTGCGCCCTGCTGGGCTTCGTCTTGGACCTGAAGCGGGACTTCCAGCCC
  GTGCGCGAGCAGATCCCGGAAGACAAGCTTTACAAAGTGTACACCTTCAACTCGGTCCGCAAGTCCATGA
  GCACAGTCATCCGCATGCCCGACGGTGGCTTCCGCCTCTTCAGCAAGGGGGCCTCAGAGATCCTCTTGAA
  AAAGTGCACCAACATCTTGAACAGCAATGGCGAACTCCGGGGCTTTCGGCCTCGGGACCGGGACGACATG
  GTGAGGAAGATCATCGAGCCGATGGCTTGCGATGGCCTCCGCACCATCTGCATCGCCTACCGGGACTTCT
  CTGCAGGCCAGGAGCCCGACTGGGACAACGAGAATGAGGTCGTGGGTGACCTCACCTGCATAGCTGTCGT
  GGGCATTGAGGACCCTGTGCGGCCCGAGGTCCCTGAAGCTATCCGAAAATGCCAGCGTGCTGGCATCACA
  GTCCGCATGGTGACTGGGGACAACATCAACACGGCCCGGGCCATCGCAGCCAAATGCGGCATCATCCAGC
  CCGGGGAGGACTTCCTGTGCCTAGAAGGGAAGGAGTTCAACCGGCGGATCCGCAATGAGAAAGGCGAGAT
  AGAACAGGAGCGGCTGGACAAGGTGTGGCCCAAGCTGAGGGTGCTGGCCCGGTCGTCTCCCACCGACAAG
  CACACACTGGTCAAAGGGATTATCGACAGCACCACTGGTGAGCAGCGGCAGGTGGTGGCTGTGACAGGGG
  ATGGCACCAACGATGGGCCGGCCCTCAAGAAGGCGGACGTGGGCTTCGCCATGGGCATCGCAGGGACCGA
  CGTGGCCAAGGAGGCCTCCGACATCATCCTGACCGATGACAACTTCACCAGCATCGTCAAGGCAGTCATG
  TGGGGCCGTAACGTCTATGACAGCATCTCCAAGTTCCTGCAGTTTCAACTGACGGTCAATGTGGTGGCTG
  TGATCGTGGCCTTCACAGGTGCCTGCATTACTCAGGACTCTCCTCTCAAAGCCGTGCAGATGTTGTGGGT
  GAACTTGATCATGGACACATTTGCCTCTCTGGCCCTGGCGACGGAGCCACCCACAGAGTCGCTGCTGCTG
  CGGAAGCCGTACGGCCGCGACAAGCCCCTCATCTCCCGCACCATGATGAAGAACATTCTGGGCCACGCCG
  TGTACCAGCTCGCCATCATCTTCACCCTGCTGTTTGTCGGGGAGCTCTTCTTCGACATCGACAGCGGGAG
  GAATGCGCCCCTGCACTCGCCACCCTCAGAGCACTACACCATCATCTTCAACACGTTCGTCATGATGCAG
  CTCTTTAACGAGATCAACGCCCGCAAGATCCACGGCGAGAGGAACGTGTTCGACGGCATCTTCAGCAACC
  CCATCTTCTGCACCATCGTTTTGGGCACTTTCGGGATTCAGATTGTCATCGTCCAGTTTGGCGGGAAGCC
  CTTCAGCTGCTCCCCACTATCCACAGAACAGTGGCTCTGGTGCCTGTTTGTTGGTGTTGGGGAGCTGGTC
  TGGGGACAGGTCATTGCCACCATCCCCACCAGCCAGCTCAAGTGCCTGAAGGAAGCCGGGCACGGGCCCG
  GGAAGGACGAGATGACCGACGAGGAGCTGGCCGAAGGCGAGGAAGAGATCGACCATGCCGAGCGGGAGCT
  CCGCAGGGGCCAGATCCTCTGGTTCCGGGGCCTGAACCGGATTCAGACGCAGATCCGGGTGGTGAAAGCG
  TTCCGTAGCTCGCTCTATGAAGGCCTGGAGAAACCAGAATCCAAGACCTCCATTCACAACTTCATGGCCA
  CGCCCGAGTTTCTGATCAATGACTACACCCACAACATCCCGCTCATTGACGACACGGACGTGGACGAGAA
  CGAGGAGCGCCTCCGGGCCCCCCCGCCCCCGTCCCCCAACCAGAACAACAACGCCATAGACAGCGGCATC
  TACCTGACCACGCATGTCACCAAGTCAGCTACCTCTTCAGTGTTTTCCTCCAGTCCCGGGAGCCCGCTCC
  ACAGCGTGGAGACGTCCCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG213737 representing NM_001001344
  Red=Cloning site Green=Tags(s)
  
  MGDMANSSIEFHPKPQQQRDVPQAGGFGCTLAELRTLMELRGAEALQKIEEAYGDVSGLCRRLKTSPTEG
  LADNTNDLEKRRQIYGQNFIPPKQPKTFLQLVWEALQDVTLIILEVAAIVSLGLSFYAPPGEESEACGNV
  SGGAEDEGEAEAGWIEGAAILLSVICVVLVTAFNDWSKEKQFRGLQSRIEQEQKFTVIRNGQLLQVPVAA
  LVVGDIAQVKYGDLLPADGVLIQANDLKIDESSLTGESDHVRKSADKDPMLLSGTHVMEGSGRMVVTAVG
  VNSQTGIIFTLLGAGGEEEEKKDKKGKQQDGAMESSQTKAKKQDGAVAMEMQPLKSAEGGEMEEREKKKA
  NAPKKEKSVLQGKLTKLAVQIGKAGLVMSAITVIILVLYFVIETFVVEGRTWLAECTPVYVQYFVKFFII
  GVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTTNRMTVVQSYL
  GDTHYKEIPAPSALTPKILDLLVHAISINSAYTTKILPPEKEGALPRQVGNKTECALLGFVLDLKRDFQP
  VREQIPEDKLYKVYTFNSVRKSMSTVIRMPDGGFRLFSKGASEILLKKCTNILNSNGELRGFRPRDRDDM
  VRKIIEPMACDGLRTICIAYRDFSAGQEPDWDNENEVVGDLTCIAVVGIEDPVRPEVPEAIRKCQRAGIT
  VRMVTGDNINTARAIAAKCGIIQPGEDFLCLEGKEFNRRIRNEKGEIEQERLDKVWPKLRVLARSSPTDK
  HTLVKGIIDSTTGEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVKAVM
  WGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTESLLL
  RKPYGRDKPLISRTMMKNILGHAVYQLAIIFTLLFVGELFFDIDSGRNAPLHSPPSEHYTIIFNTFVMMQ
  LFNEINARKIHGERNVFDGIFSNPIFCTIVLGTFGIQIVIVQFGGKPFSCSPLSTEQWLWCLFVGVGELV
  WGQVIATIPTSQLKCLKEAGHGPGKDEMTDEELAEGEEEIDHAERELRRGQILWFRGLNRIQTQIRVVKA
  FRSSLYEGLEKPESKTSIHNFMATPEFLINDYTHNIPLIDDTDVDENEERLRAPPPPSPNQNNNAIDSGI
  YLTTHVTKSATSSVFSSSPGSPLHSVETSL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001001344
• ORF Size: 3660 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001001344.1, NP_001001344.1
• RefSeq Size: 6438 bp
• RefSeq ORF: 3663 bp
• Locus ID: 492
• Cytogenetics: Xq28
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Calcium signaling pathway
• Gene Summary: 'The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 3. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]'