ATP2B3 (NM_001001344) Human Untagged Clone

CAT#: SC300169

ATP2B3 (untagged)-Human ATPase, Ca++ transporting, plasma membrane 3 (ATP2B3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATP2B3
• Synonyms: CFAP39; CLA2; OPCA; PMCA3; PMCA3a; SCAX1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001001344, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGCGACATGGCCAATAGTTCCATCGAGTTCCACCCCAAGCCCCAGCAGCAGCGGGATGTCCCCCAGG
  CTGGAGGCTTTGGGTGCACGCTGGCGGAGCTGCGCACCCTCATGGAGCTGCGAGGGGCCGAGGCGCTGCA
  GAAGATCGAGGAGGCCTACGGGGATGTCAGCGGGCTCTGCCGGAGGCTGAAGACCTCACCCACAGAGGGC
  CTGGCGGACAACACCAATGACCTGGAGAAGCGCAGGCAGATCTACGGGCAGAACTTCATCCCCCCAAAGC
  AACCCAAGACCTTCCTGCAGCTGGTGTGGGAGGCCCTGCAGGACGTGACCCTCATCATCCTGGAGGTGGC
  TGCCATCGTCTCTCTGGGCCTCTCGTTCTATGCGCCGCCAGGAGAGGAGAGTGAAGCCTGTGGGAATGTG
  TCGGGAGGCGCAGAAGATGAGGGCGAGGCCGAAGCTGGCTGGATCGAGGGGGCTGCCATCCTGCTGTCCG
  TCATCTGTGTGGTGCTGGTCACGGCCTTCAATGACTGGAGCAAGGAGAAGCAGTTCCGAGGCCTGCAGAG
  CCGAATTGAGCAGGAGCAGAAGTTCACGGTCATCCGGAACGGGCAGCTCCTCCAGGTCCCCGTGGCTGCG
  CTGGTGGTGGGGGACATTGCCCAGGTCAAGTACGGCGACCTGCTGCCAGCCGACGGCGTGCTCATCCAGG
  CCAATGACCTCAAGATCGACGAGAGCTCCCTGACGGGCGAGTCTGACCACGTGCGCAAGTCAGCTGACAA
  AGATCCCATGCTGCTCTCAGGCACTCATGTCATGGAAGGTTCTGGAAGAATGGTGGTGACCGCCGTTGGC
  GTGAATTCCCAGACAGGCATCATCTTCACGCTGCTTGGAGCTGGCGGAGAGGAGGAAGAGAAGAAAGATA
  AGAAAGGCAAGCAGCAGGATGGGGCCATGGAGAGTAGCCAGACCAAAGCTAAGAAGCAGGATGGTGCAGT
  GGCCATGGAGATGCAGCCCCTGAAGAGCGCGGAGGGTGGGGAGATGGAGGAGCGGGAGAAGAAGAAAGCC
  AACGCACCCAAAAAGGAGAAGTCTGTCCTTCAGGGGAAGCTCACAAAGCTAGCCGTGCAGATCGGGAAAG
  CAGGGCTGGTGATGTCTGCCATCACCGTCATCATCCTGGTCCTCTACTTTGTGATTGAGACGTTTGTCGT
  GGAAGGCCGGACATGGCTGGCAGAGTGCACGCCGGTCTATGTACAATACTTCGTGAAGTTCTTCATCATT
  GGTGTCACTGTGCTGGTCGTGGCTGTCCCAGAGGGCCTGCCTCTTGCTGTCACCATCTCCTTAGCTTACT
  CTGTCAAGAAAATGATGAAAGACAACAACCTGGTGCGCCACCTGGATGCCTGCGAGACCATGGGCAACGC
  CACAGCCATCTGCTCCGACAAGACGGGCACGCTCACCACCAACCGTATGACCGTGGTCCAGTCCTACCTA
  GGAGACACCCACTACAAAGAGATTCCGGCCCCCAGCGCCCTGACCCCTAAGATCCTCGACCTCCTGGTCC
  ATGCCATCTCCATCAACAGTGCCTATACCACCAAAATACTACCTCCTGAGAAGGAAGGCGCCCTCCCACG
  CCAGGTGGGCAATAAGACGGAGTGCGCCCTGCTGGGCTTCGTCTTGGACCTGAAGCGGGACTTCCAGCCC
  GTGCGCGAGCAGATCCCGGAAGACAAGCTTTACAAAGTGTACACCTTCAACTCGGTCCGCAAGTCCATGA
  GCACAGTCATCCGCATGCCCGACGGTGGCTTCCGCCTCTTCAGCAAGGGGGCCTCAGAGATCCTCTTGAA
  AAAGTGCACCAACATCTTGAACAGCAATGGCGAACTCCGGGGCTTTCGGCCTCGGGACCGGGACGACATG
  GTGAGGAAGATCATCGAGCCGATGGCTTGCGATGGCCTCCGCACCATCTGCATCGCCTACCGGGACTTCT
  CTGCAGGCCAGGAGCCCGACTGGGACAACGAGAATGAGGTCGTGGGTGACCTCACCTGCATAGCTGTCGT
  GGGCATTGAGGACCCTGTGCGGCCCGAGGTCCCTGAAGCTATCCGAAAATGCCAGCGTGCTGGCATCACA
  GTCCGCATGGTGACTGGGGACAACATCAACACGGCCCGGGCCATCGCAGCCAAATGCGGCATCATCCAGC
  CCGGGGAGGACTTCCTGTGCCTAGAAGGGAAGGAGTTCAACCGGCGGATCCGCAATGAGAAAGGCGAGAT
  AGAACAGGAGCGGCTGGACAAGGTGTGGCCCAAGCTGAGGGTGCTGGCCCGGTCGTCTCCCACCGACAAG
  CACACACTGGTCAAAGGGATTATCGACAGCACCACTGGTGAGCAGCGGCAGGTGGTGGCTGTGACAGGGG
  ATGGCACCAACGATGGGCCGGCCCTCAAGAAGGCGGACGTGGGCTTCGCCATGGGCATCGCAGGGACCGA
  CGTGGCCAAGGAGGCCTCCGACATCATCCTGACCGATGACAACTTCACCAGCATCGTCAAGGCAGTCATG
  TGGGGCCGTAACGTCTATGACAGCATCTCCAAGTTCCTGCAGTTTCAACTGACGGTCAATGTGGTGGCTG
  TGATCGTGGCCTTCACAGGTGCCTGCATTACTCAGGACTCTCCTCTCAAAGCCGTGCAGATGTTGTGGGT
  GAACTTGATCATGGACACATTTGCCTCTCTGGCCCTGGCGACGGAGCCACCCACAGAGTCGCTGCTGCTG
  CGGAAGCCGTACGGCCGCGACAAGCCCCTCATCTCCCGCACCATGATGAAGAACATTCTGGGCCACGCCG
  TGTACCAGCTCGCCATCATCTTCACCCTGCTGTTTGTCGGGGAGCTCTTCTTCGACATCGACAGCGGGAG
  GAATGCGCCCCTGCACTCGCCACCCTCAGAGCACTACACCATCATCTTCAACACGTTCGTCATGATGCAG
  CTCTTTAACGAGATCAACGCCCGCAAGATCCACGGCGAGAGGAACGTGTTCGACGGCATCTTCAGCAACC
  CCATCTTCTGCACCATCGTTTTGGGCACTTTCGGGATTCAGATTGTCATCGTCCAGTTTGGCGGGAAGCC
  CTTCAGCTGCTCCCCACTATCCACAGAACAGTGGCTCTGGTGCCTGTTTGTTGGTGTTGGGGAGCTGGTC
  TGGGGACAGGTCATTGCCACCATCCCCACCAGCCAGCTCAAGTGCCTGAAGGAAGCCGGGCACGGGCCCG
  GGAAGGACGAGATGACCGACGAGGAGCTGGCCGAAGGCGAGGAAGAGATCGACCATGCCGAGCGGGAGCT
  CCGCAGGGGCCAGATCCTCTGGTTCCGGGGCCTGAACCGGATTCAGACGCAGATCCGGGTGGTGAAAGCG
  TTCCGTAGCTCGCTCTATGAAGGCCTGGAGAAACCAGAATCCAAGACCTCCATTCACAACTTCATGGCCA
  CGCCCGAGTTTCTGATCAATGACTACACCCACAACATCCCGCTCATTGACGACACGGACGTGGACGAGAA
  CGAGGAGCGCCTCCGGGCCCCCCCGCCCCCGTCCCCCAACCAGAACAACAACGCCATAGACAGCGGCATC
  TACCTGACCACGCATGTCACCAAGTCAGCTACCTCTTCAGTGTTTTCCTCCAGTCCCGGGAGCCCGCTCC
  ACAGCGTGGAGACGTCCCTCTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001001344
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001001344.2, NP_001001344.1
• RefSeq Size: 6420 bp
• RefSeq ORF: 3663 bp
• Locus ID: 492
• Cytogenetics: Xq28
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Calcium signaling pathway
• Gene Summary: 'The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 3. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) lacks an exon in the coding region, which leads to a frameshift, compared to variant 1. The resulting isoform (3b) contains a longer and distinct C-terminus compared to isoform 3a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.