Motilin receptor (MLNR) (NM_001507) Human Tagged ORF Clone

CAT#: RG214391

MLNR (GFP-tagged) - Human motilin receptor (MLNR)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MLNR
• Synonyms: GPR38; MTLR1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214391 representing NM_001507
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAGCCCCTGGAACGGCAGCGACGGCCCCGAGGGGGCGCGGGAGCCGCCGTGGCCCGCGCTGCCGC
  CTTGCGACGAGCGCCGCTGCTCGCCCTTTCCCCTGGGGGCGCTGGTGCCGGTGACCGCTGTGTGCCTGTG
  CCTGTTCGTCGTCGGGGTGAGCGGCAACGTGGTGACCGTGATGCTGATCGGGCGCTACCGGGACATGCGG
  ACCACCACCAACTTGTACCTGGGCAGCATGGCCGTGTCCGACCTACTCATCCTGCTCGGGCTGCCGTTCG
  ACCTGTACCGCCTCTGGCGCTCGCGGCCCTGGGTGTTCGGGCCGCTGCTCTGCCGCCTGTCCCTCTACGT
  GGGCGAGGGCTGCACCTACGCCACGCTGCTGCACATGACCGCGCTCAGCGTCGAGCGCTACCTGGCCATC
  TGCCGCCCGCTCCGCGCCCGCGTCTTGGTCACCCGGCGCCGCGTCCGCGCGCTCATCGCTGTGCTCTGGG
  CCGTGGCGCTGCTCTCTGCCGGTCCCTTCTTGTTCCTGGTGGGCGTCGAGCAGGACCCCGGCATCTCCGT
  AGTCCCGGGCCTCAATGGCACCGCGCGGATCGCCTCCTCGCCTCTCGCCTCGTCGCCGCCTCTCTGGCTC
  TCGCGGGCGCCACCGCCGTCCCCGCCGTCGGGGCCCGAGACCGCGGAGGCCGCGGCGCTGTTCAGCCGCG
  AATGCCGGCCGAGCCCCGCGCAGCTGGGCGCGCTGCGTGTCATGCTGTGGGTCACCACCGCCTACTTCTT
  CCTGCCCTTTCTGTGCCTCAGCATCCTCTACGGGCTCATCGGGCGGGAGCTGTGGAGCAGCCGGCGGCCG
  CTGCGAGGCCCGGCCGCCTCGGGGCGGGAGAGAGGCCACCGGCAGACCGTCCGCGTCCTGCTGGTGGTGG
  TTCTGGCATTTATAATTTGCTGGTTGCCCTTCCACGTTGGCAGAATCATTTACATAAACACGGAAGATTC
  GCGGATGATGTACTTCTCTCAGTACTTTAACATCGTCGCTCTGCAACTTTTCTATCTGAGCGCATCTATC
  AACCCAATCCTCTACAACCTCATTTCAAAGAAGTACAGAGCGGCGGCCTTTAAACTGCTGCTCGCAAGGA
  AGTCCAGGCCGAGAGGCTTCCACAGAAGCAGGGACACTGCGGGGGAAGTTGCAGGGGACACTGGAGGAGA
  CACGGTGGGCTACACCGAGACAAGCGCTAACGTGAAGACGATGGGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214391 representing NM_001507
  Red=Cloning site Green=Tags(s)
  
  MGSPWNGSDGPEGAREPPWPALPPCDERRCSPFPLGALVPVTAVCLCLFVVGVSGNVVTVMLIGRYRDMR
  TTTNLYLGSMAVSDLLILLGLPFDLYRLWRSRPWVFGPLLCRLSLYVGEGCTYATLLHMTALSVERYLAI
  CRPLRARVLVTRRRVRALIAVLWAVALLSAGPFLFLVGVEQDPGISVVPGLNGTARIASSPLASSPPLWL
  SRAPPPSPPSGPETAEAAALFSRECRPSPAQLGALRVMLWVTTAYFFLPFLCLSILYGLIGRELWSSRRP
  LRGPAASGRERGHRQTVRVLLVVVLAFIICWLPFHVGRIIYINTEDSRMMYFSQYFNIVALQLFYLSASI
  NPILYNLISKKYRAAAFKLLLARKSRPRGFHRSRDTAGEVAGDTGGDTVGYTETSANVKTMG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001507
• ORF Size: 1236 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001507.1, NP_001498.1
• RefSeq Size: 1239 bp
• RefSeq ORF: 1239 bp
• Locus ID: 2862
• Cytogenetics: 13q14.2
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: 'Motilin is a 22 amino acid peptide hormone expressed throughout the gastrointestinal (GI) tract. The protein encoded by this gene is a motilin receptor which is a member of the G-protein coupled receptor 1 family. This member is a multi-pass transmembrane protein, and is an important therapeutic target for the treatment of hypomotility disorders. [provided by RefSeq, Aug 2011]'