Neurofascin (NFASC) (NM_015090) Human Tagged ORF Clone

CAT#: RG214686

NFASC (GFP-tagged) - Human neurofascin (NFASC), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NFASC
• Synonyms: NEDCPMD; NF; NRCAML
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214686 representing NM_015090
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGGCAGCCACCGCCGCCCTGGGTCCATGCAGCCTTCCTCCTCTGCCTCCTCAGTCTTGGCGGAG
  CCATCGAAATTCCTATGGATCTGACGCAGCCGCCAACCATCACCAAGCAGTCAGCGAAGGATCACATCGT
  GGACCCCCGTGATAACATCCTGATTGAGTGTGAAGCAAAAGGGAACCCTGCCCCCAGCTTCCACTGGACA
  CGAAACAGCAGATTCTTCAACATCGCCAAGGACCCCCGGGTGTCCATGAGGAGGAGGTCTGGGACCCTGG
  TGATTGACTTCCGCAGTGGCGGGCGGCCGGAGGAATATGAGGGGGAATATCAGTGCTTCGCCCGCAACAA
  ATTTGGCACGGCCCTGTCCAATAGGATCCGCCTGCAGGTGTCTAAATCTCCTCTGTGGCCCAAGGAAAAC
  CTAGACCCTGTCGTGGTCCAAGAGGGCGCTCCTTTGACGCTCCAGTGCAACCCCCCGCCTGGACTTCCAT
  CCCCGGTCATCTTCTGGATGAGCAGCTCCATGGAGCCCATCACCCAAGACAAACGTGTCTCTCAGGGCCA
  TAACGGAGACCTATACTTCTCCAACGTGATGCTGCAGGACATGCAGACCGACTACAGTTGTAACGCCCGC
  TTCCACTTCACCCACACCATCCAGCAGAAGAACCCTTTCACCCTCAAGGTCCTCACCAACCACCCTTATA
  ATGACTCGTCCTTAAGAAACCACCCTGACATGTACAGTGCCCGAGGAGTTGCAGAAAGAACACCAAGCTT
  CATGTATCCCCAGGGCACCGCGAGCAGCCAGATGGTGCTTCGTGGCATGGACCTCCTGCTGGAATGCATC
  GCCTCCGGGGTCCCAACACCAGACATCGCATGGTACAAGAAAGGTGGGGACCTCCCATCTGATAAGGCCA
  AGTTTGAGAACTTTAATAAGGCCCTGCGTATCACAAATGTCTCTGAGGAAGACTCCGGGGAGTATTTCTG
  CCTGGCCTCCAACAAGATGGGCAGCATCCGGCACACGATCTCGGTGAGAGTAAAGGCTGCTCCCTACTGG
  CTGGACGAACCCAAGAACCTTATTCTGGCTCCTGGCGAGGATGGGAGACTGGTGTGTCGAGCCAATGGAA
  ACCCCAAACCCACTGTCCAGTGGATGGTGAATGGGGAACCTTTGCAATCGGCACCACCTAACCCAAACCG
  TGAGGTGGCCGGAGACACCATCATCTTCCGGGACACCCAGATCAGCAGCAGGGCTGTGTACCAGTGCAAC
  ACCTCCAACGAGCATGGCTACCTGCTGGCCAACGCCTTTGTCAGTGTGCTGGATGTGCCGCCTCGGATGC
  TGTCGCCCCGGAACCAGCTCATTCGAGTGATTCTTTACAACCGGACGCGGCTGGACTGCCCTTTCTTTGG
  GTCTCCCATCCCCACACTGCGATGGTTTAAGAATGGGCAAGGAAGCAACCTGGATGGTGGCAACTACCAT
  GTTTATGAGAACGGCAGTCTGGAAATTAAGATGATCCGCAAAGAGGACCAGGGCATCTACACCTGTGTCG
  CCACCAACATCCTGGGCAAAGCTGAAAACCAAGTCCGCCTGGAGGTCAAAGACCCCACCAGGATCTACCG
  GATGCCCGAGGACCAGGTGGCCAGAAGGGGCACCACGGTGCAGCTGGAGTGTCGGGTGAAGCACGACCCC
  TCCCTGAAACTCACCGTCTCCTGGCTGAAGGATGACGAGCCGCTCTATATTGGAAACAGGATGAAGAAGG
  AAGACGACTCCCTGACCATCTTTGGGGTGGCAGAGCGGGACCAGGGCAGTTACACGTGTGTCGCCAGCAC
  CGAGCTAGACCAAGACCTGGCCAAGGCCTACCTCACCGTGCTAGGACGGCCAGACCGGCCCCGGGACCTG
  GAGCTGACCGACCTGGCCGAGAGGAGCGTGCGGCTGACCTGGATCCCCGGGGATGCTAACAACAGCCCCA
  TCACAGACTACGTCGTCCAGTTTGAAGAAGACCAGTTCCAACCTGGGGTCTGGCATGACCATTCCAAGTA
  CCCCGGCAGCGTTAACTCAGCCGTCCTCCGGCTGTCCCCGTATGTCAACTACCAGTTCCGTGTCATTGCC
  ATCAACGAGGTTGGGAGCAGCCACCCCAGCCTCCCATCCGAGCGCTACCGAACCAGTGGAGCACCCCCCG
  AGTCCAATCCTGGTGACGTGAAGGGAGAGGGGACCAGAAAGAACAACATGGAGATCACGTGGACGCCCAT
  GAATGCCACCTCGGCCTTTGGCCCCAACCTGCGCTACATTGTCAAGTGGAGGCGGAGAGAGACTCGAGAG
  GCCTGGAACAACGTCACAGTGTGGGGCTCTCGCTACGTGGTGGGGCAGACCCCAGTCTACGTGCCCTATG
  AGATCCGAGTCCAGGCTGAAAATGACTTCGGGAAGGGCCCTGAGCCAGAGTCCGTCATCGGTTACTCCGG
  AGAAGATTATCCCAGGGCTGCGCCCACTGAAGTTAAAGTCCGAGTCATGAACAGCACAGCCATCAGCCTT
  CAGTGGAACCGCGTCTACTCCGACACGGTCCAGGGCCAGCTCAGAGAGTACCGAGCCTACTACTGGAGGG
  AGAGCAGCTTGCTGAAGAACCTGTGGGTGTCTCAGAAGAGACAGCAAGCCAGCTTCCCTGGTGACCGCCT
  CCGTGGCGTGGTGTCCCGCCTCTTCCCCTACAGTAACTACAAGCTGGAGATGGTTGTGGTCAATGGGAGA
  GGTGATGGGCCTCGCAGTGAGACCAAGGAGTTCACCACCCCGGAAGGAGTACCCAGTGCCCCTAGGCGTT
  TCCGAGTCCGGCAGCCCAACCTGGAGACAATCAACCTGGAATGGGATCATCCTGAGCATCCAAATGGGAT
  CATGATTGGATACACTCTCAAATATGTGGCCTTTAACGGGACCAAAGTAGGAAAGCAGATAGTGGAAAAC
  TTCTCTCCCAATCAGACCAAGTTCACGGTGCAAAGAACGGACCCCGTGTCACGCTACCGCTTTACCCTCA
  GCGCCAGGACGCAGGTGGGCTCTGGGGAAGCCGTCACAGAGGAGTCACCAGCACCCCCGAATGAAGCTTA
  CACCAACAACCAAGCGGACATCGCCACCCAGGGCTGGTTCATTGGGCTTATGTGCGCCATCGCCCTCCTG
  GTGCTGATCCTGCTCATCGTCTGTTTCATCAAGAGGAGTCGCGGCGGCAAGTACCCAGTACGAGAAAAGA
  AGGATGTTCCCCTTGGCCCTGAAGACCCCAAGGAAGAGGATGGCTCATTTGACTATAGTGATGAGGACAA
  CAAGCCCCTGCAGGGCAGTCAGACATCTCTGGACGGCACCATCAAGCAGCAGGAGAGTGACGACAGCCTG
  GTGGACTATGGCGAGGGTGGCGAGGGTCAGTTCAATGAAGACGGCTCCTTCATCGGCCAGTACACGGTCA
  AAAAGGACAAGGAGGAAACAGAGGGCAACGAAAGCTCAGAGGCCACGTCACCTGTCAATGCTATCTACTC
  TCTGGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214686 representing NM_015090
  Red=Cloning site Green=Tags(s)
  
  MARQPPPPWVHAAFLLCLLSLGGAIEIPMDLTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWT
  RNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKEN
  LDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNAR
  FHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECI
  ASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYW
  LDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCN
  TSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYH
  VYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDP
  SLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLGRPDRPRDL
  ELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAVLRLSPYVNYQFRVIA
  INEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRE
  AWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAPTEVKVRVMNSTAISL
  QWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLFPYSNYKLEMVVVNGR
  GDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKYVAFNGTKVGKQIVEN
  FSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEAYTNNQADIATQGWFIGLMCAIALL
  VLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSL
  VDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015090
• ORF Size: 3507 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015090.3, NP_055905.2
• RefSeq Size: 10138
• RefSeq ORF: 3510
• Locus ID: 23114
• Protein Families: Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)
• Gene Summary: This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined. [provided by RefSeq, May 2009]