NALP1 (NLRP1) (NM_033007) Human Tagged ORF Clone

CAT#: RG215205

NLRP1 (GFP-tagged) - Human NLR family, pyrin domain containing 1 (NLRP1), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NLRP1
• Synonyms: AIADK; CARD7; CIDED; CLR17.1; DEFCAP; DEFCAP-L/S; MSPC; NAC; NALP1; PP1044; SLEV1; VAMAS1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG215205 representing NM_033007
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGCGGAGCCTGGGGCCGCCTGGCCTGTTACTTGGAGTTCCTGAAGAAGGAGGAGCTGAAGGAGT
  TCCAGCTTCTGCTCGCCAATAAAGCGCACTCCAGGAGCTCTTCGGGTGAGACACCCGCTCAGCCAGAGAA
  GACGAGTGGCATGGAGGTGGCCTCGTACCTGGTGGCTCAGTATGGGGAGCAGCGGGCCTGGGACCTAGCC
  CTCCATACCTGGGAGCAGATGGGGCTGAGGTCACTGTGCGCCCAAGCCCAGGAAGGGGCAGGCCACTCTC
  CCTCATTCCCCTACAGCCCAAGTGAACCCCACCTGGGGTCTCCCAGCCAACCCACCTCCACCGCAGTGCT
  AATGCCCTGGATCCATGAATTGCCGGCGGGGTGCACCCAGGGCTCAGAGAGAAGGGTTTTGAGACAGCTG
  CCTGACACATCTGGACGCCGCTGGAGAGAAATCTCTGCCTCACTCCTCTACCAAGCTCTTCCAAGCTCCC
  CAGACCATGAGTCTCCAAGCCAGGAGTCACCCAACGCCCCCACATCCACAGCAGTGCTGGGGAGCTGGGG
  ATCCCCACCTCAGCCCAGCCTAGCACCCAGAGAGCAGGAGGCTCCTGGGACCCAATGGCCTCTGGATGAA
  ACGTCAGGAATTTACTACACAGAAATCAGAGAAAGAGAGAGAGAGAAATCAGAGAAAGGCAGGCCCCCAT
  GGGCAGCGGTGGTAGGAACGCCCCCACAGGCGCACACCAGCCTACAGCCCCACCACCACCCATGGGAGCC
  TTCTGTGAGAGAGAGCCTCTGTTCCACATGGCCCTGGAAAAATGAGGATTTTAACCAAAAATTCACACAG
  CTGCTACTTCTACAAAGACCTCACCCCAGAAGCCAAGATCCCCTGGTCAAGAGAAGCTGGCCTGATTATG
  TGGAGGAGAATCGAGGACATTTAATTGAGATCAGAGACTTATTTGGCCCAGGCCTGGATACCCAAGAACC
  TCGCATAGTCATACTGCAGGGGGCTGCTGGAATTGGGAAGTCAACACTGGCCAGGCAGGTGAAGGAAGCC
  TGGGGGAGAGGCCAGCTGTATGGGGACCGCTTCCAGCATGTCTTCTACTTCAGCTGCAGAGAGCTGGCCC
  AGTCCAAGGTGGTGAGTCTCGCTGAGCTCATCGGAAAAGATGGGACAGCCACTCCGGCTCCCATTAGACA
  GATCCTGTCTAGGCCAGAGCGGCTGCTCTTCATCCTCGATGGTGTAGATGAGCCAGGATGGGTCTTGCAG
  GAGCCGAGTTCTGAGCTCTGTCTGCACTGGAGCCAGCCACAGCCGGCGGATGCACTGCTGGGCAGTTTGC
  TGGGGAAAACTATACTTCCCGAGGCATCCTTCCTGATCACGGCTCGGACCACAGCTCTGCAGAACCTCAT
  TCCTTCTTTGGAGCAGGCACGTTGGGTAGAGGTCCTGGGGTTCTCTGAGTCCAGCAGGAAGGAATATTTC
  TACAGATATTTCACAGATGAAAGGCAAGCAATTAGAGCCTTTAGGTTGGTCAAATCAAACAAAGAGCTCT
  GGGCCCTGTGTCTTGTGCCCTGGGTGTCCTGGCTGGCCTGCACTTGCCTGATGCAGCAGATGAAGCGGAA
  GGAAAAACTCACACTGACTTCCAAGACCACCACAACCCTCTGTCTACATTACCTTGCCCAGGCTCTCCAA
  GCTCAGCCATTGGGACCCCAGCTCAGAGACCTCTGCTCTCTGGCTGCTGAGGGCATCTGGCAAAAAAAGA
  CCCTTTTCAGTCCAGATGACCTCAGGAAGCATGGGTTAGATGGGGCCATCATCTCCACCTTCTTGAAGAT
  GGGTATTCTTCAAGAGCACCCCATCCCTCTGAGCTACAGCTTCATTCACCTCTGTTTCCAAGAGTTCTTT
  GCAGCAATGTCCTATGTCTTGGAGGATGAGAAGGGGAGAGGTAAACATTCTAATTGCATCATAGATTTGG
  AAAAGACGCTAGAAGCATATGGAATACATGGCCTGTTTGGGGCATCAACCACACGTTTCCTATTGGGCCT
  GTTAAGTGATGAGGGGGAGAGAGAGATGGAGAACATCTTTCACTGCCGGCTGTCTCAGGGGAGGAACCTG
  ATGCAGTGGGTCCCGTCCCTGCAGCTGCTGCTGCAGCCACACTCTCTGGAGTCCCTCCACTGCTTGTACG
  AGACTCGGAACAAAACGTTCCTGACACAAGTGATGGCCCATTTCGAAGAAATGGGCATGTGTGTAGAAAC
  AGACATGGAGCTCTTAGTGTGCACTTTCTGCATTAAATTCAGCCGCCACGTGAAGAAGCTTCAGCTGATT
  GAGGGCAGGCAGCACAGATCAACATGGAGCCCCACCATGGTAGTCCTGTTCAGGTGGGTCCCAGTCACAG
  ATGCCTATTGGCAGATTCTCTTCTCCGTCCTCAAGGTCACCAGAAACCTGAAGGAGCTGGACCTAAGTGG
  AAACTCGCTGAGCCACTCTGCAGTGAAGAGTCTTTGTAAGACCCTGAGACGCCCTCGCTGCCTCCTGGAG
  ACCCTGCGGTTGGCTGGCTGTGGCCTCACAGCTGAGGACTGCAAGGACCTTGCCTTTGGGCTGAGAGCCA
  ACCAGACCCTGACCGAGCTGGACCTGAGCTTCAATGTGCTCACGGATGCTGGAGCCAAACACCTTTGCCA
  GAGACTGAGACAGCCGAGCTGCAAGCTACAGCGACTGCAGCTGGTCAGCTGTGGCCTCACGTCTGACTGC
  TGCCAGGACCTGGCCTCTGTGCTTAGTGCCAGCCCCAGCCTGAAGGAGCTAGACCTGCAGCAGAACAACC
  TGGATGACGTTGGCGTGCGACTGCTCTGTGAGGGGCTCAGGCATCCTGCCTGCAAACTCATACGCCTGGG
  GAAACCAAGTGTGATGACCCCTACTGAGGGCCTGGATACGGGAGAGATGAGTAATAGCACATCCTCACTC
  AAGCGGCAGAGACTCGGATCAGAGAGGGCGGCTTCCCATGTTGCTCAGGCTAATCTCAAACTCCTGGACG
  TGAGCAAGATCTTCCCAATTGCTGAGATTGCAGAGGAAAGCTCCCCAGAGGTAGTACCGGTGGAACTCTT
  GTGCGTGCCTTCTCCTGCCTCTCAAGGGGACCTGCATACGAAGCCTTTGGGGACTGACGATGACTTCTGG
  GGCCCCACGGGGCCTGTGGCTACTGAGGTAGTTGACAAAGAAAAGAACTTGTACCGAGTTCACTTCCCTG
  TAGCTGGCTCCTACCGCTGGCCCAACACGGGTCTCTGCTTTGTGATGAGAGAAGCGGTGACCGTTGAGAT
  TGAATTCTGTGTGTGGGACCAGTTCCTGGGTGAGATCAACCCACAGCACAGCTGGATGGTGGCAGGGCCT
  CTGCTGGACATCAAGGCTGAGCCTGGAGCTGTGGAAGCTGTGCACCTCCCTCACTTTGTGGCTCTCCAAG
  GGGGCCATGTGGACACATCCCTGTTCCAAATGGCCCACTTTAAAGAGGAGGGGATGCTCCTGGAGAAGCC
  AGCCAGGGTGGAGCTGCATCACATAGTTCTGGAAAACCCCAGCTTCTCCCCCTTGGGAGTCCTCCTGAAA
  ATGATCCATAATGCCCTGCGCTTCATTCCCGTCACCTCTGTGGTGTTGCTTTACCACCGCGTCCATCCTG
  AGGAAGTCACCTTCCACCTCTACCTGATCCCAAGTGACTGCTCCATTCGGAAGGAACTGGAGCTCTGCTA
  TCGAAGCCCTGGAGAAGACCAGCTGTTCTCGGAGTTCTACGTTGGCCACTTGGGATCAGGGATCAGGCTG
  CAAGTGAAAGACAAGAAAGATGAGACTCTGGTGTGGGAGGCCTTGGTGAAACCAGGAGATCTCATGCCTG
  CAACTACTCTGATCCCTCCAGCCCGCATAGCCGTACCTTCACCTCTGGATGCCCCGCAGTTGCTGCACTT
  TGTGGACCAGTATCGAGAGCAGCTGATAGCCCGAGTGACATCGGTGGAGGTTGTCTTGGACAAACTGCAT
  GGACAGGTGCTGAGCCAGGAGCAGTACGAGAGGGTGCTGGCTGAGAACACGAGGCCCAGCCAGATGCGGA
  AGCTGTTCAGCTTGAGCCAGTCCTGGGACCGGAAGTGCAAAGATGGACTCTACCAAGCCCTGAAGGAGAC
  CCATCCTCACCTCATTATGGAACTCTGGGAGAAGGGCAGCAAAAAGGGACTCCTGCCACTCAGCAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG215205 representing NM_033007
  Red=Cloning site Green=Tags(s)
  
  MAGGAWGRLACYLEFLKKEELKEFQLLLANKAHSRSSSGETPAQPEKTSGMEVASYLVAQYGEQRAWDLA
  LHTWEQMGLRSLCAQAQEGAGHSPSFPYSPSEPHLGSPSQPTSTAVLMPWIHELPAGCTQGSERRVLRQL
  PDTSGRRWREISASLLYQALPSSPDHESPSQESPNAPTSTAVLGSWGSPPQPSLAPREQEAPGTQWPLDE
  TSGIYYTEIREREREKSEKGRPPWAAVVGTPPQAHTSLQPHHHPWEPSVRESLCSTWPWKNEDFNQKFTQ
  LLLLQRPHPRSQDPLVKRSWPDYVEENRGHLIEIRDLFGPGLDTQEPRIVILQGAAGIGKSTLARQVKEA
  WGRGQLYGDRFQHVFYFSCRELAQSKVVSLAELIGKDGTATPAPIRQILSRPERLLFILDGVDEPGWVLQ
  EPSSELCLHWSQPQPADALLGSLLGKTILPEASFLITARTTALQNLIPSLEQARWVEVLGFSESSRKEYF
  YRYFTDERQAIRAFRLVKSNKELWALCLVPWVSWLACTCLMQQMKRKEKLTLTSKTTTTLCLHYLAQALQ
  AQPLGPQLRDLCSLAAEGIWQKKTLFSPDDLRKHGLDGAIISTFLKMGILQEHPIPLSYSFIHLCFQEFF
  AAMSYVLEDEKGRGKHSNCIIDLEKTLEAYGIHGLFGASTTRFLLGLLSDEGEREMENIFHCRLSQGRNL
  MQWVPSLQLLLQPHSLESLHCLYETRNKTFLTQVMAHFEEMGMCVETDMELLVCTFCIKFSRHVKKLQLI
  EGRQHRSTWSPTMVVLFRWVPVTDAYWQILFSVLKVTRNLKELDLSGNSLSHSAVKSLCKTLRRPRCLLE
  TLRLAGCGLTAEDCKDLAFGLRANQTLTELDLSFNVLTDAGAKHLCQRLRQPSCKLQRLQLVSCGLTSDC
  CQDLASVLSASPSLKELDLQQNNLDDVGVRLLCEGLRHPACKLIRLGKPSVMTPTEGLDTGEMSNSTSSL
  KRQRLGSERAASHVAQANLKLLDVSKIFPIAEIAEESSPEVVPVELLCVPSPASQGDLHTKPLGTDDDFW
  GPTGPVATEVVDKEKNLYRVHFPVAGSYRWPNTGLCFVMREAVTVEIEFCVWDQFLGEINPQHSWMVAGP
  LLDIKAEPGAVEAVHLPHFVALQGGHVDTSLFQMAHFKEEGMLLEKPARVELHHIVLENPSFSPLGVLLK
  MIHNALRFIPVTSVVLLYHRVHPEEVTFHLYLIPSDCSIRKELELCYRSPGEDQLFSEFYVGHLGSGIRL
  QVKDKKDETLVWEALVKPGDLMPATTLIPPARIAVPSPLDAPQLLHFVDQYREQLIARVTSVEVVLDKLH
  GQVLSQEQYERVLAENTRPSQMRKLFSLSQSWDRKCKDGLYQALKETHPHLIMELWEKGSKKGLLPLSS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033007
• ORF Size: 4197 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_033007.3, NP_127500.1
• RefSeq Size: 5401
• RefSeq ORF: 4200
• Locus ID: 22861
• Protein Families: Druggable Genome
• Protein Pathways: NOD-like receptor signaling pathway
• Gene Summary: This gene encodes a member of the Ced-4 family of apoptosis proteins. Ced-family members contain a caspase recruitment domain (CARD) and are known to be key mediators of programmed cell death. The encoded protein contains a distinct N-terminal pyrin-like motif, which is possibly involved in protein-protein interactions. This protein interacts strongly with caspase 2 and weakly with caspase 9. Overexpression of this gene was demonstrated to induce apoptosis in cells. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]