RYK (NM_002958) Human Tagged ORF Clone

CAT#: RG215509

RYK (GFP-tagged) - Human RYK receptor-like tyrosine kinase (RYK), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RYK
• Synonyms: D3S3195; JTK5; JTK5A; RYK1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG215509 representing NM_002958
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGTGGGGCGGCGCGGCTGGGGCGGCCGGGCCGGAGTTGCCTCCCGGGGGCCCGCGGCCTGAGGGCCC
  CGCCGCCGCCGCCGCTGCTGCTTCTGCTTGCGCTGTTGCCGCTGCTGCCCGCGCCTGGCGCTGCCGCCGC
  CCCCGCCCCGCGGCCCCCGGAGCTGCAGTCGGCTTCCGCGGGGCCCAGCGTGAGTCTCTACCTGAGCGAG
  GACGAGGTGCGCCGGCTGATCGGTCTTGATGCAGAACTTTATTATGTGAGAAATGACCTTATTAGTCACT
  ACGCTCTATCCTTTAGTCTGTTAGTACCCAGTGAGACAAATTTCCTGCACTTCACCTGGCATGCGAAGTC
  CAAGGTTGAATATAAGCTGGGATTCCAAGTGGACAATGTTTTGGCAATGGATATGCCCCAGGTCAACATT
  TCTGTTCAGGGGGAAGTTCCACGCACTTTATCAGTGTTTCGGGTAGAGCTTTCCTGTACTGGCAAAGTAG
  ATTCTGAAGTTATGATACTAATGCAGCTCAACTTGACAGTAAATTCTTCAAAAAATTTTACCGTCTTAAA
  TTTTAAACGAAGGAAAATGTGCTACAAAAAACTTGAAGAAGTAAAAACTTCAGCCTTGGACAAAAACACT
  AGCAGAACTATTTATGATCCTGTACATGCAGCTCCAACCACTTCTACGCGTGTGTTTTATATTAGTGTAG
  GGGTTTGTTGTGCAGTAATATTTCTCGTAGCAATAATATTAGCTGTTTTGCACCTTCATAGTATGAAAAG
  GATTGAACTGGATGACAGCATTAGTGCCAGCAGTAGTTCCCAAGGGCTGTCTCAGCCATCCACCCAGACG
  ACTCAGTATCTGAGAGCAGACACGCCCAACAATGCAACTCCTATCACCAGTTATCCTACCTTGCGGATAG
  AGAAGAACGACTTGAGAAGTGTCACTCTTTTGGAGGCCAAAGGCAAGGTGAAGGATATAGCAATATCCAG
  AGAGAGGATAACTCTAAAAGATGTACTCCAAGAAGGTACTTTTGGGCGTATTTTCCATGGGATTTTAATA
  GATGAAAAAGATCCAAATAAAGAAAAACAAGCATTTGTCAAAACAGTTAAAGATCAAGCTTCTGAAATTC
  AGGTGACAATGATGCTCACTGAAAGTTGTAAGCTGCGAGGTCTTCATCACAGAAATCTTCTTCCTATTAC
  TCATGTGTGTATAGAAGAAGGAGAAAAGCCCATGGTGATATTGCCTTACATGAATTGGGGGAATCTTAAA
  TTGTTTTTACGACAGTGCAAGTTAGTAGAGGCCAATAATCCACAGGCAATTTCTCAGCAAGACCTGGTAC
  ACATGGCTATTCAGATTGCCTGTGGAATGAGCTACCTGGCCAGAAGGGAAGTCATCCACAAAGACCTGGC
  TGCCAGGAACTGTGTCATTGATGACACACTTCAAGTTAAGATCACAGACAATGCCCTCTCCAGAGACTTG
  TTCCCCATGGACTATCACTGTCTGGGGGACAATGAAAACAGGCCAGTTCGTTGGATGGCTCTTGAAAGTC
  TGGTTAATAACGAGTTCTCTAGCGCTAGTGATGTGTGGGCCTTTGGAGTGACGCTGTGGGAACTCATGAC
  TCTGGGCCAGACTCCCTACGTGGACATTGACCCCTTCGAGATGGCCGCATACCTGAAAGATGGTTACCGA
  ATAGCCCAGCCAATCAACTGTCCTGATGAATTATTTGCTGTGATGGCCTGTTGCTGGGCCTTAGATCCAG
  AGGAGAGGCCCAAGTTTCAGCAGCTGGTACAGTGCCTAACAGAGTTTCATGCAGCCCTGGGGGCCTACGT
  C
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG215509 representing NM_002958
  Red=Cloning site Green=Tags(s)
  
  MRGAARLGRPGRSCLPGARGLRAPPPPPLLLLLALLPLLPAPGAAAAPAPRPPELQSASAGPSVSLYLSE
  DEVRRLIGLDAELYYVRNDLISHYALSFSLLVPSETNFLHFTWHAKSKVEYKLGFQVDNVLAMDMPQVNI
  SVQGEVPRTLSVFRVELSCTGKVDSEVMILMQLNLTVNSSKNFTVLNFKRRKMCYKKLEEVKTSALDKNT
  SRTIYDPVHAAPTTSTRVFYISVGVCCAVIFLVAIILAVLHLHSMKRIELDDSISASSSSQGLSQPSTQT
  TQYLRADTPNNATPITSYPTLRIEKNDLRSVTLLEAKGKVKDIAISRERITLKDVLQEGTFGRIFHGILI
  DEKDPNKEKQAFVKTVKDQASEIQVTMMLTESCKLRGLHHRNLLPITHVCIEEGEKPMVILPYMNWGNLK
  LFLRQCKLVEANNPQAISQQDLVHMAIQIACGMSYLARREVIHKDLAARNCVIDDTLQVKITDNALSRDL
  FPMDYHCLGDNENRPVRWMALESLVNNEFSSASDVWAFGVTLWELMTLGQTPYVDIDPFEMAAYLKDGYR
  IAQPINCPDELFAVMACCWALDPEERPKFQQLVQCLTEFHAALGAYV
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002958
• ORF Size: 1821 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_002958.2, NP_002949.2
• RefSeq Size: 2951
• RefSeq ORF: 1824
• Locus ID: 6259
• Domains: pkinase, TyrKc, S_TKc, WIF
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Gene Summary: The protein encoded by this gene is an atypical member of the family of growth factor receptor protein tyrosine kinases, differing from other members at a number of conserved residues in the activation and nucleotide binding domains. This gene product belongs to a subfamily whose members do not appear to be regulated by phosphorylation in the activation segment. It has been suggested that mediation of biological activity by recruitment of a signaling-competent auxiliary protein may occur through an as yet uncharacterized mechanism. The encoded protein has a leucine-rich extracellular domain with a WIF-type Wnt binding region, a single transmembrane domain, and an intracellular tyrosine kinase domain. This protein is involved in stimulating Wnt signaling pathways such as the regulation of axon pathfinding. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2012]