RYK (NM_002958) Human Untagged Clone

CAT#: SC323660

RYK (untagged)-Kinase deficient mutant (K234M) of Human RYK receptor-like tyrosine kinase (RYK), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RYK
• Synonyms: D3S3195; JTK5; JTK5A; RYK1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002958, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCGTGGGGCGGCGCGGCTGGGGCGGCCGGGCCGGAGTTGCCTCCCGGGGGCCCGCGGCCTGAGGGCCC
  CGCCGCCGCCGCCGCTGCTGCTTCTGCTTGCGCTGTTGCCGCTGCTGCCCGCGCCTGGCGCTGCCGCCGC
  CCCCGCCCCGCGGCCCCCGGAGCTGCAGTCGGCTTCCGCGGGGCCCAGCGTGAGTCTCTACCTGAGCGAG
  GACGAGGTGCGCCGGCTGATCGGTCTTGATGCAGAACTTTATTATGTGAGAAATGACCTTATTAGTCACT
  ACGCTCTATCCTTTAGTCTGTTAGTACCCAGTGAGACAAATTTCCTGCACTTCACCTGGCATGCGAAGTC
  CAAGGTTGAATATAAGCTGGGATTCCAAGTGGACAATGTTTTGGCAATGGATATGCCCCAGGTCAACATT
  TCTGTTCAGGGGGAAGTTCCACGCACTTTATCAGTGTTTCGGGTAGAGCTTTCCTGTACTGGCAAAGTAG
  ATTCTGAAGTTATGATACTAATGCAGCTCAACTTGACAGTAAATTCTTCAAAAAATTTTACCGTCTTAAA
  TTTTAAACGAAGGAAAATGTGCTACAAAAAACTTGAAGAAGTAAAAACTTCAGCCTTGGACAAAAACACT
  AGCAGAACTATTTATGATCCTGTACATGCAGCTCCAACCACTTCTACGCGTGTGTTTTATATTAGTGTAG
  GGGTTTGTTGTGCAGTAATATTTCTCGTAGCAATAATATTAGCTGTTTTGCACCTTCATAGTATGAAAAG
  GATTGAACTGGATGACAGCATTAGTGCCAGCAGTAGTTCCCAAGGGCTGTCTCAGCCATCCACCCAGACG
  ACTCAGTATCTGAGAGCAGACACGCCCAACAATGCAACTCCTATCACCAGTTATCCTACCTTGCGGATAG
  AGAAGAACGACTTGAGAAGTGTCACTCTTTTGGAGGCCAAAGGCAAGGTGAAGGATATAGCAATATCCAG
  AGAGAGGATAACTCTAAAAGATGTACTCCAAGAAGGTACTTTTGGGCGTATTTTCCATGGGATTTTAATA
  GATGAAAAAGATCCAAATAAAGAAAAACAAGCATTTGTCAAAACAGTTAAAGATCAAGCTTCTGAAATTC
  AGGTGACAATGATGCTCACTGAAAGTTGTAAGCTGCGAGGTCTTCATCACAGAAATCTTCTTCCTATTAC
  TCATGTGTGTATAGAAGAAGGAGAAAAGCCCATGGTGATATTGCCTTACATGAATTGGGGGAATCTTAAA
  TTGTTTTTACGACAGTGCAAGTTAGTAGAGGCCAATAATCCACAGGCAATTTCTCAGCAAGACCTGGTAC
  ACATGGCTATTCAGATTGCCTGTGGAATGAGCTACCTGGCCAGAAGGGAAGTCATCCACAAAGACCTGGC
  TGCCAGGAACTGTGTCATTGATGACACACTTCAAGTTAAGATCACAGACAATGCCCTCTCCAGAGACTTG
  TTCCCCATGGACTATCACTGTCTGGGGGACAATGAAAACAGGCCAGTTCGTTGGATGGCTCTTGAAAGTC
  TGGTTAATAACGAGTTCTCTAGCGCTAGTGATGTGTGGGCCTTTGGAGTGACGCTGTGGGAACTCATGAC
  TCTGGGCCAGACTCCCTACGTGGACATTGACCCCTTCGAGATGGCCGCATACCTGAAAGATGGTTACCGA
  ATAGCCCAGCCAATCAACTGTCCTGATGAATTATTTGCTGTGATGGCCTGTTGCTGGGCCTTAGATCCAG
  AGGAGAGGCCCAAGTTTCAGCAGCTGGTACAGTGCCTAACAGAGTTTCATGCAGCCCTGGGGGCCTACGT
  CTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_002958 unedited
  CCCGCCGTTTGAGCAATGGGCGGTAGGCGTGTACGGTTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTG
  AACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGCGTCCCGCG
  GGCAGCCCCCAACTCCGGACGATGCAGCGAGGAGGCGGCGGCGGCGGCGCGCTCCGGAGGTCTGAAGCCA
  CTGCTGGCTGCCACGGGCCGGCGGTGCGATGAGCTGGGCAGCCGCCCCCGCCCCGCGGCCCCCGGAGCTG
  CAGTCGGCTTCCGCGGGGCCCAGCGTGAGTCTCTACCTGAGCGAGGACGAGTGCGCCGCTGATCGTCTTG
  ATGCAGACTTTATTATGTGAAAAATGACCTTATTAATTCACTAACGCCTCCTATCCCTTTATTCTTGGTT
  ATTACCCATTGAACACAAATTTCCCTGCACCTTCCACCTTGGCATGCCCGAAGTCCCAGGGTGGGAATAA
  TAGGCCTTGGAAATTCCAGGGTGGGACAAGGTTTTTGCCAAGGAGATATGCCCCCAGGTCACACATTTTT
  GGTTTCGGGGGGAAATTTCCCCCCCCTTTTTTAGTGTGTTTCGGGGAAACGTTTTCCGGGGCGGGGCAAG
  TTATATTTGTAAGTTTTGAAATCAAAGGGCCCCCTAACTTTGGCGGGAAATTTTTCTCAAAAATTTTTTC
  CCCTTTTAATTTTTTTAAACAAAGGGGAAAGGGGGCTAAAAAAAATTTTGAAAAATATTAAAACTCTTTC
  CCCTGTGGGAAAAAAACCCCTCACAAAACACCAATAAAAAACCGGACAACAGGCGCCCCCCCCTTTTTCT
  AGCCGCGGGGTAAAAAAAAGGCGGGGGGGTCTGGGTCCACAAATTTTTTCTCACACCAATTATTATCTGT
  TTTGCCCCCCACATATAGAGAGAATATACTGTCGAGACAGG
  
  Kinase domain sequence

  >SC323660 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  GMTCCAGATGTACTTTTGGGCGTATTTTCCATGGGATTTTAATAGATGAAAAAGATCCAAATAAAGAAAA
  ACAAGCATTTGTCATGACAGTTAAAGATCAAGCTTCTGAAATTCAGGTGACAATGATGCTCACTGAAAGT
  TGTAAGCTGCGAGGTCTTCATCACAGAAATCTTCTTCCTATTACTCATGTGTGTATAGAAGAAGGAGAAA
  AGCCCATGGTGATATTGCCTTACATGAATTGGGGGAATCTTAAAT
  
• Restriction Sites: Please inquire
• ACCN: NM_002958
• ORF Size: 1824 bp
• Insert Size: 3100
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_002958.2, NP_002949.2
• RefSeq Size: 2951
• RefSeq ORF: 1824
• Locus ID: 6259
• Domains: pkinase, TyrKc, S_TKc, WIF
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Gene Summary: The protein encoded by this gene is an atypical member of the family of growth factor receptor protein tyrosine kinases, differing from other members at a number of conserved residues in the activation and nucleotide binding domains. This gene product belongs to a subfamily whose members do not appear to be regulated by phosphorylation in the activation segment. It has been suggested that mediation of biological activity by recruitment of a signaling-competent auxiliary protein may occur through an as yet uncharacterized mechanism. The encoded protein has a leucine-rich extracellular domain with a WIF-type Wnt binding region, a single transmembrane domain, and an intracellular tyrosine kinase domain. This protein is involved in stimulating Wnt signaling pathways such as the regulation of axon pathfinding. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2012]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1, resulting in a shorter protein (isoform 2).