gamma Adaptin (AP1G1) (NM_001128) Human Tagged ORF Clone

CAT#: RG216694

AP1G1 (GFP-tagged) - Human adaptor-related protein complex 1, gamma 1 subunit (AP1G1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: AP1G1
• Synonyms: ADTG; CLAPG1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG216694 representing NM_001128
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGCCCCCATCAGATTGCGGGAGCTGATCCGGACCATCCGGACAGCCCGAACCCAAGCTGAAGAAC
  GAGAAATGATCCAGAAAGAATGTGCTGCAATCCGGTCATCTTTTAGAGAAGAAGACAATACATACCGATG
  TCGGAATGTGGCAAAATTACTGTATATGCACATGCTGGGCTACCCTGCTCACTTTGGACAGTTGGAGTGC
  CTCAAGCTTATTGCCTCTCAAAAATTTACAGACAAACGCATTGGCTATTTAGGGGCAATGCTGCTGTTAG
  ATGAAAGACAAGATGTCCATCTTCTCATGACCAACTGTATCAAGAATGATCTTAATCATAGCACGCAATT
  CGTACAGGGGTTAGCACTTTGTACCCTCGGCTGCATGGGCTCCTCAGAGATGTGCAGAGATCTTGCAGGA
  GAGGTAGAGAAGCTCCTGAAAACCTCCAACTCTTACTTAAGAAAAAAGGCAGCACTGTGTGCTGTTCATG
  TCATCAGGAAAGTTCCTGAACTTATGGAGATGTTTTTACCAGCAACAAAAAATTTATTGAATGAGAAGAA
  CCATGGTGTCCTCCACACATCTGTAGTCCTCCTCACAGAAATGTGTGAGCGAAGCCCAGACATGCTTGCG
  CATTTCAGAAAGCTTGTGCCCCAATTAGTTCGTATTTTAAAGAACCTCATCATGTCCGGATATTCACCAG
  AACATGATGTTTCTGGTATCAGTGACCCCTTTTTGCAGGTACGAATTTTGCGGTTATTAAGAATTTTAGG
  ACGAAATGATGATGATTCAAGTGAAGCTATGAATGATATATTAGCACAGGTTGCCACTAATACTGAGACT
  AGTAAAAATGTAGGAAATGCTATTCTTTATGAAACGGTTTTGACTATCATGGATATTAAGTCAGAGAGTG
  GATTGCGAGTCCTAGCCATAAATATCCTGGGTCGTTTCTTATTGAACAATGACAAGAATATTAGATATGT
  GGCTCTGACATCTTTGTTGAAGACTGTACAGACAGATCATAATGCAGTACAGAGGCACAGAAGCACAATT
  GTGGACTGTCTTAAAGATTTGGATGTCTCAATAAAACGGCGTGCAATGGAATTGAGTTTTGCCCTGGTAA
  ATGGGAATAATATCCGAGGCATGATGAAAGAATTACTTTATTTTCTGGATTCGTGTGAGCCAGAATTTAA
  AGCAGACTGTGCATCTGGAATCTTTCTTGCTGCAGAAAAGTATGCACCTTCCAAACGATGGCATATAGAC
  ACAATTATGCGTGTTTTGACAACGGCAGGAAGTTATGTTCGTGATGATGCAGTCCCCAATTTAATCCAGT
  TAATAACTAATAGTGTGGAGATGCATGCCTATACTGTCCAGCGCCTGTACAAAGCAATTCTTGGTGATTA
  TTCTCAACAACCTTTGGTACAAGTGGCTGCATGGTGTATAGGTGAATATGGTGATCTTCTTGTATCTGGC
  CAGTGTGAAGAGGAAGAGCCTATTCAGGTAACAGAGGATGAAGTGTTGGATATTTTAGAAAGTGTCCTAA
  TCTCTAATATGTCCACCTCTGTGACACGAGGTTATGCCCTCACTGCCATTATGAAGCTTTCCACTCGATT
  CACTTGTACTGTAAACCGAATTAAGAAAGTGGTTTCCATCTACGGAAGCAGCATTGATGTGGAACTCCAG
  CAGAGGGCAGTAGAATATAATGCACTTTTCAAGAAATATGACCACATGAGGTCTGCCCTACTTGAGAGAA
  TGCCTGTCATGGAAAAAGTGACCACAAATGGCCCTACTGAGATTGTGCAGACAAATGGAGAGACAGAACC
  AGCTCCACTAGAGACCAAACCGCCACCCTCTGGGCCACAGCCCACCAGCCAGGCCAATGATTTATTGGAT
  TTGTTGGGAGGAAATGACATAACACCTGTTATTCCAACTGCGCCTACAAGCAAACCATCTTCTGCTGGTG
  GAGAACTTCTTGATTTGCTGGGAGACATCAACCTTACAGGTGCTCCAGCTGCTGCTCCTGCCCCTGCCTC
  AGTCCCACAGATATCCCAGCCCCCCTTCTTGTTGGATGGGCTTTCATCACAGCCTCTCTTCAATGATATT
  GCTGCAGGCATCCCCTCCATCACAGCATACAGTAAGAATGGCTTGAAGATAGAATTCACCTTTGAACGGT
  CAAATACCAACCCCAGTGTAACAGTGATAACGATACAGGCCTCCAACAGCACAGAGCTAGATATGACGGA
  CTTTGTTTTCCAAGCTGCAGTACCAAAGACATTCCAGCTGCAGCTCTTGTCTCCTAGCAGCAGCATTGTC
  CCAGCATTTAACACGGGGACCATCACACAAGTCATTAAAGTTCTGAACCCTCAGAAGCAACAGCTGCGAA
  TGCGGATCAAGCTTACATATAATCACAAGGGCTCAGCAATGCAAGATCTAGCAGAGGTGAACAACTTTCC
  CCCTCAGTCCTGGCAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG216694 representing NM_001128
  Red=Cloning site Green=Tags(s)
  
  MPAPIRLRELIRTIRTARTQAEEREMIQKECAAIRSSFREEDNTYRCRNVAKLLYMHMLGYPAHFGQLEC
  LKLIASQKFTDKRIGYLGAMLLLDERQDVHLLMTNCIKNDLNHSTQFVQGLALCTLGCMGSSEMCRDLAG
  EVEKLLKTSNSYLRKKAALCAVHVIRKVPELMEMFLPATKNLLNEKNHGVLHTSVVLLTEMCERSPDMLA
  HFRKLVPQLVRILKNLIMSGYSPEHDVSGISDPFLQVRILRLLRILGRNDDDSSEAMNDILAQVATNTET
  SKNVGNAILYETVLTIMDIKSESGLRVLAINILGRFLLNNDKNIRYVALTSLLKTVQTDHNAVQRHRSTI
  VDCLKDLDVSIKRRAMELSFALVNGNNIRGMMKELLYFLDSCEPEFKADCASGIFLAAEKYAPSKRWHID
  TIMRVLTTAGSYVRDDAVPNLIQLITNSVEMHAYTVQRLYKAILGDYSQQPLVQVAAWCIGEYGDLLVSG
  QCEEEEPIQVTEDEVLDILESVLISNMSTSVTRGYALTAIMKLSTRFTCTVNRIKKVVSIYGSSIDVELQ
  QRAVEYNALFKKYDHMRSALLERMPVMEKVTTNGPTEIVQTNGETEPAPLETKPPPSGPQPTSQANDLLD
  LLGGNDITPVIPTAPTSKPSSAGGELLDLLGDINLTGAPAAAPAPASVPQISQPPFLLDGLSSQPLFNDI
  AAGIPSITAYSKNGLKIEFTFERSNTNPSVTVITIQASNSTELDMTDFVFQAAVPKTFQLQLLSPSSSIV
  PAFNTGTITQVIKVLNPQKQQLRMRIKLTYNHKGSAMQDLAEVNNFPPQSWQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001128
• ORF Size: 2466 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001128.5, NP_001119.3
• RefSeq Size: 6841 bp
• RefSeq ORF: 2469 bp
• Locus ID: 164
• Cytogenetics: 16q22.2
• Domains: Adaptin_N, Alpha_adaptinC2
• Protein Pathways: Lysosome
• Gene Summary: 'Adaptins are important components of clathrin-coated vesicles transporting ligand-receptor complexes from the plasma membrane or from the trans-Golgi network to lysosomes. The adaptin family of proteins is composed of four classes of molecules named alpha, beta-, beta prime- and gamma- adaptins. Adaptins, together with medium and small subunits, form a heterotetrameric complex called an adaptor, whose role is to promote the formation of clathrin-coated pits and vesicles. The protein encoded by this gene is a gamma-adaptin protein and it belongs to the adaptor complexes large subunits family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'