gamma Adaptin (AP1G1) (NM_001128) Human Untagged Clone

CAT#: SC119413

AP1G1 (untagged)-Human adaptor-related protein complex 1, gamma 1 subunit (AP1G1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AP1G1
• Synonyms: ADTG; CLAPG1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119413 sequence for NM_001128 edited (data generated by NextGen Sequencing)
  ATGCCAGCCCCCATCAGATTGCGGGAGCTGATCCGGACCATCCGGACAGCCCGAACCCAA
  GCTGAAGAACGAGAAATGATCCAGAAAGAATGTGCTGCAATCCGGTCATCTTTTAGAGAA
  GAAGACAATACATACCGATGTCGGAATGTGGCAAAATTACTGTATATGCACATGCTGGGC
  TACCCTGCTCACTTTGGACAGTTGGAGTGCCTCAAGCTTATTGCCTCTCAAAAATTTACA
  GACAAACGCATTGGCTATTTAGGGGCAATGCTGCTGTTAGATGAAAGACAAGATGTCCAT
  CTTCTCATGACCAACTGTATCAAGAATGATCTTAATCATAGCACGCAATTCGTACAGGGG
  TTAGCACTTTGTACCCTCGGCTGCATGGGCTCCTCAGAGATGTGCAGAGATCTTGCAGGA
  GAGGTAGAGAAGCTCCTGAAAACCTCCAACTCTTACTTAAGAAAAAAGGCAGCACTGTGT
  GCTGTTCATGTCATCAGGAAAGTTCCTGAACTTATGGAGATGTTTTTACCAGCAACAAAA
  AATTTATTGAATGAGAAGAACCATGGTGTCCTCCACACATCTGTAGTCCTCCTCACAGAA
  ATGTGTGAGCGAAGCCCAGACATGCTTGCGCATTTCAGAAAGCTTGTGCCCCAATTAGTT
  CGTATTTTAAAGAACCTCATCATGTCCGGATATTCACCAGAACATGATGTTTCTGGTATC
  AGTGACCCCTTTTTGCAGGTACGAATTTTGCGGTTATTAAGAATTTTAGGACGAAATGAT
  GATGATTCAAGTGAAGCTATGAATGATATATTAGCACAGGTTGCCACTAATACTGAGACT
  AGTAAAAATGTAGGAAATGCTATTCTTTATGAAACGGTTTTGACTATCATGGATATTAAG
  TCAGAGAGTGGATTGCGAGTCCTAGCCATAAATATCCTGGGTCGTTTCTTATTGAACAAT
  GACAAGAATATTAGATATGTGGCTCTGACATCTTTGTTGAAGACTGTACAGACAGATCAT
  AATGCAGTACAGAGGCACAGAAGCACAATTGTGGACTGTCTTAAAGATTTGGATGTCTCA
  ATAAAACGGCGTGCAATGGAATTGAGTTTTGCCCTGGTAAATGGGAATAATATCCGAGGC
  ATGATGAAAGAATTACTTTATTTTCTGGATTCGTGTGAGCCAGAATTTAAAGCAGACTGT
  GCATCTGGAATCTTTCTTGCTGCAGAAAAGTATGCACCTTCCAAACGATGGCATATAGAC
  ACAATTATGCGTGTTTTGACAACGGCAGGAAGTTATGTTCGTGATGATGCAGTCCCCAAT
  TTAATCCAGTTAATAACTAATAGTGTGGAGATGCATGCCTATACTGTCCAGCGCCTGTAC
  AAAGCAATTCTTGGTGATTATTCTCAACAACCTTTGGTACAAGTGGCTGCATGGTGTATA
  GGTGAATATGGTGATCTTCTTGTATCTGGCCAGTGTGAAGAGGAAGAGCCTATTCAGGTA
  ACAGAGGATGAAGTGTTGGATATTTTAGAAAGTGTCCTAATCTCTAATATGTCCACCTCT
  GTGACACGAGGTTATGCCCTCACTGCCATTATGAAGCTTTCCACTCGATTCACTTGTACT
  GTAAACCGAATTAAGAAAGTGGTTTCCATCTACGGAAGCAGCATTGATGTGGAACTCCAG
  CAGAGGGCAGTAGAATATAATGCACTTTTCAAGAAATATGACCACATGAGGTCTGCCCTA
  CTTGAGAGAATGCCTGTCATGGAAAAAGTGACCACAAATGGCCCTACTGAGATTGTGCAG
  ACAAATGGAGAGACAGAACCAGCTCCACTAGAGACCAAACCGCCACCCTCTGGGCCACAG
  CCCACCAGCCAGGCCAATGATTTATTGGATTTGTTGGGAGGAAATGACATAACACCTGTT
  ATTCCAACTGCGCCTACAAGCAAACCATCTTCTGCTGGTGGAGAACTTCTTGATTTGCTG
  GGAGACATCAACCTTACAGGTGCTCCAGCTGCTGCTCCTGCCCCTGCCTCAGTCCCACAG
  ATATCCCAGCCCCCCTTCTTGTTGGATGGGCTTTCATCACAGCCTCTCTTCAATGATATT
  GCTGCAGGCATCCCCTCCATCACAGCATACAGTAAGAATGGCTTGAAGATAGAATTCACC
  TTTGAACGGTCAAATACCAACCCCAGTGTAACAGTGATAACGATACAGGCCTCCAACAGC
  ACAGAGCTAGATATGACGGACTTTGTTTTCCAAGCTGCAGTACCAAAGACATTCCAGCTG
  CAGCTCTTGTCTCCTAGCAGCAGCATTGTCCCAGCATTTAACACGGGGACCATCACACAA
  GTCATTAAAGTTCTGAACCCTCAGAAGCAACAGCTGCGAATGCGGATCAAGCTTACATAT
  AATCACAAGGGCTCAGCAATGCAAGATCTAGCAGAGGTGAACAACTTTCCCCCTCAGTCC
  TGGCAATGA
  
  Clone variation with respect to NM_001128.5
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001128 unedited
  NGGTTCGAATATTTGTATACGACTCACTATTAGGGCGGCCGCGATTCGGCACGAGTGCCG
  CCGCCCCCGGAGCTGCTGGTTTCATTCGAGGTTTCGGGCCGTTTCAGGGGTCCAGTCCCA
  GGCTGTGGGTGTTGAGAAGAGAAGACAGAGAAGCTGGGCCAGGAGAGGCCGGGAGCCCCA
  GGGGCTGCTCCGACTTCTGGAGGGCCGACCCCGAGGCCTCGGACCGGGAGGTTCCGCGCG
  CCGCGGCGTTCCGTGTTGTGGCGTTGCCCCGCCGCTCCTGGCCCTGCGGACGTCAGCGTC
  TAATAATTCTCCCGGTCTTCCTGATTGCCTGAAAGAGTCGGGACGGTTCGGTTGATTGTG
  TTTCCCGGTTAGGACTCGGTGTAGGAGATCCATCGAACCACTCGCTCAGGGATGCCAGCC
  CCCATCAGATTGCGGGAGCTGATCCGGACCATCCGGACAGCCCGAACCCAAGCTGAAGAA
  CGAGAAATGATCCAGAAAGAATGTGCTGCAATCCGGTCATCTTTTAGAGAAGAAGACAAT
  ACATACCGATGTCGGAATGTGGCAAAATTACTGTATATGCACATGCTGGGCTACCCTGCT
  CACTTTGGANCAGTTGGAGTGCCTCAAGCTTATTGCCTCTCAAAAATTTACAGACAAACG
  CATTGGCTATTTANGGGCAATGCTGCTGTTAGATGAAAGACNAGATGTCCATCTTCTCAT
  GACCAACTGTATCAAGAATGATCTTAATCATAGCACGCAATTCGTACCGGGGGTTAGCAC
  TTTGTACCCTCGGCTGCATGGGCTCTCAANAATGGGNCANAGATCTGCAGGGAGAGGTAN
  AGAAGCTCCTGANACCTCCACTCTTACTTAGAAAAGCGCACTGGGGTGCTGGTATGCTCN
  GGGGAAGTCCTGATATAGGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001128 unedited
  ATTTTTAACCGTGTGGTCTTTTATTTAAGTGCCAGTGTTACAGTTACAACACAAATGTTC
  CAGTTAGAAGGAATTCAAACGGAATGCCAAGGTCCAAGCCAGGCTCAAAAAATAAAAAGG
  GAGGTTTGGAGTAATAAATAAAATGACTCCAATACTCACTCTTCCTAAGGGCAAAGGTCC
  TTTTGATACAGAGTCTGATCTTTGAAACTGGTGAACTCCTCTTCCACCCATTACCATAGT
  TCAAACAGGCAAGTTATGGGCTTAGGACCACTTTAAAATTTGGGGGGGGAATAGGGTCAT
  TAATAACTATGAATATATCTTTTAAAAGGTGACCATTTTGCACTTTAAAGGGAATCAATT
  TTGAAAATCATGGAAACTATTCATGACTACAGCTAAAAAATGGCGAAAAAGGGGAGCTGG
  AAAAGCCTTGGAAGTTTCTATTACAAATAGAGCACCATATCCTTCATGCCAAATCTCAAC
  AAAAGCTCTTTTTAACTCCATCTGTCCAGTGTTAACAAATAAACTCGCAAGGTCTGACCA
  GTTCTTGGTAACAAACATACATGTGTGTGTCTGTGTGTATACAGCAATGCACAAAAAAGG
  CTACCAGGAGCCTAATGCCTCTTTCAAACATTGGGGGAACCAGTAGAAAAAGGCAGGGCT
  CCCTAATGTCCATTATTACATTTCCATTCCGAATGCCAGATGTTAAAAGTGCCTGAAGAT
  GGTAACCCAGCTAGTGAGGAATAAATACCCCACCTTGCCCAGTCCACAGAGGAACAACAG
  TNGAAAGAGGGGGCACTTCTTTGCTGCAAAGACAAATGAGTGTTTTTTCCCATGGATCGC
  AGTCCTTTCCTCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001128
• Insert Size: 4280 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001128.5, NP_001119.3
• RefSeq Size: 6841 bp
• RefSeq ORF: 2469 bp
• Locus ID: 164
• Cytogenetics: 16q22.2
• Domains: Adaptin_N, Alpha_adaptinC2
• Protein Pathways: Lysosome
• Gene Summary: 'Adaptins are important components of clathrin-coated vesicles transporting ligand-receptor complexes from the plasma membrane or from the trans-Golgi network to lysosomes. The adaptin family of proteins is composed of four classes of molecules named alpha, beta-, beta prime- and gamma- adaptins. Adaptins, together with medium and small subunits, form a heterotetrameric complex called an adaptor, whose role is to promote the formation of clathrin-coated pits and vesicles. The protein encoded by this gene is a gamma-adaptin protein and it belongs to the adaptor complexes large subunits family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) lacks an in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a.