DPP6 (NM_001936) Human Tagged ORF Clone

CAT#: RG216875

DPP6 (GFP-tagged) - Human dipeptidyl-peptidase 6 (DPP6), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DPP6
• Synonyms: DPL1; DPPX; MRD33; VF2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG216875 representing NM_001936
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCACAGCCAAGGAGCCAAGCGCTTCGGGGAAATCCGTGCAGCAGCAGGAACAGGAGCTGGTGGGGA
  GTAACCCTCCGCAGAGGAATTGGAAAGGAATAGCAATTGCACTGCTTGTCATTCTGGTCATCTGCTCCTT
  GATCGTCACCTCGGTCATACTTCTGACACCAGCGGAAGATAATAGTCTGTCTCAAAAGAAGAAGGTCACT
  GTAGAAGATCTCTTCAGTGAAGACTTCAAAATTCATGACCCCGAGGCTAAGTGGATAAGTGATACAGAAT
  TCATCTACAGAGAACAGAAAGGAACAGTGAGACTGTGGAATGTTGAAACAAATACTTCTACTGTCTTAAT
  AGAAGGCAAAAAAATTGAATCATTAAGAGCCATCAGATATGAAATATCTCCAGATAGAGAGTATGCACTT
  TTTTCATACAATGTGGAACCCATATATCAACACTCGTATACTGGATATTACGTCCTGAGCAAAATTCCTC
  ATGGGGATCCTCAAAGTCTGGACCCACCAGAAGTCAGCAATGCAAAGCTTCAGTATGCAGGATGGGGCCC
  TAAAGGCCAACAGCTGATATTTATTTTTGAAAACAATATCTACTACTGTGCACATGTCGGGAAACAGGCC
  ATCCGTGTGGTCTCCACTGGCAAGGAAGGTGTGATTTACAATGGCCTCAGTGACTGGCTGTATGAAGAGG
  AGATTTTGAAGACACACATCGCACACTGGTGGTCTCCGGATGGCACGAGACTCGCCTACGCCGCCATCAA
  TGATTCCCGTGTCCCCATCATGGAGCTCCCAACTTACACCGGCTCCATCTACCCCACCGTGAAGCCCTAC
  CACTATCCCAAGGCTGGAAGTGAGAACCCCAGCATTTCCCTACACGTTATTGGCTTAAATGGACCCACCC
  ATGATCTGGAGATGATGCCGCCTGATGATCCACGGATGAGGGAGTACTACATCACCATGGTGAAGTGGGC
  CACCAGCACCAAGGTCGCCGTGACCTGGCTGAACCGGGCGCAGAACGTGTCCATCCTCACCCTCTGCGAC
  GCCACCACGGGGGTCTGCACGAAGAAACACGAGGATGAAAGTGAGGCCTGGCTCCACAGACAGAATGAAG
  AACCTGTGTTCTCCAAGGATGGCCGAAAGTTTTTCTTCATCAGAGCCATCCCCCAGGGAGGACGAGGGAA
  ATTCTATCACATCACGGTGTCCTCGTCCCAGCCCAACAGCAGCAACGACAACATCCAGTCCATCACCTCC
  GGGGACTGGGACGTGACCAAGATCCTAGCCTACGATGAGAAGGGGAATAAGATCTACTTCCTGAGCACGG
  AGGACCTGCCTCGGAGACGACAACTCTACAGTGCCAACACGGTGGGCAACTTCAACAGGCAGTGCCTCTC
  CTGTGACCTGGTTGAGAACTGCACCTACTTCAGCGCTTCCTTCAGCCATAGCATGGACTTCTTCCTGCTC
  AAGTGCGAAGGTCCTGGTGTTCCTATGGTGACGGTGCACAACACAACAGATAAGAAAAAAATGTTTGACC
  TAGAAACAAATGAACATGTCAAGAAGGCCATAAATGACCGACAGATGCCTAAAGTGGAATACAGGGACAT
  TGAGATTGATGATTACAACCTGCCCATGCAGATACTGAAGCCAGCAACCTTCACCGACACCACCCACTAC
  CCTCTGCTCCTGGTGGTGGATGGCACCCCGGGCAGCCAGAGTGTAGCTGAGAAGTTCGAGGTGAGCTGGG
  AGACGGTGATGGTGAGCAGCCACGGCGCGGTGGTGGTAAAGTGTGACGGCCGTGGCAGCGGCTTCCAAGG
  GACCAAGCTCCTGCACGAAGTGAGGCGGCGGCTGGGCTTGCTGGAGGAGAAGGACCAGATGGAGGCCGTG
  CGGACGATGCTGAAGGAGCAGTACATTGACAGGACGCGCGTGGCCGTGTTTGGGAAGGATTACGGTGGCT
  ACCTGAGCACCTACATCCTCCCAGCAAAGGGAGAAAATCAAGGCCAGACATTCACCTGCGGCTCTGCTCT
  CTCTCCAATAACAGACTTCAAACTCTATGCCTCTGCGTTTTCCGAGAGGTACTTGGGCCTCCATGGACTT
  GACAACAGAGCATACGAGATGACCAAGGTAGCCCATCGAGTCTCCGCGCTGGAAGAACAGCAGTTCCTGA
  TCATTCATCCCACTGCCGATGAAAAAATTCATTTCCAGCACACAGCAGAACTCATTACACAACTAATTAG
  GGGAAAGGCTAATTACAGCTTACAGATTTACCCGGACGAAAGCCATTACTTTACCAGCTCCAGCCTCAAA
  CAGCATCTGTACCGGTCCATCATCAACTTCTTCGTGGAATGCTTCAGGATCCAGGACAAACTGCCGACAG
  TCACAGCGAAAGAGGACGAGGAGGAGGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG216875 representing NM_001936
  Red=Cloning site Green=Tags(s)
  
  MTTAKEPSASGKSVQQQEQELVGSNPPQRNWKGIAIALLVILVICSLIVTSVILLTPAEDNSLSQKKKVT
  VEDLFSEDFKIHDPEAKWISDTEFIYREQKGTVRLWNVETNTSTVLIEGKKIESLRAIRYEISPDREYAL
  FSYNVEPIYQHSYTGYYVLSKIPHGDPQSLDPPEVSNAKLQYAGWGPKGQQLIFIFENNIYYCAHVGKQA
  IRVVSTGKEGVIYNGLSDWLYEEEILKTHIAHWWSPDGTRLAYAAINDSRVPIMELPTYTGSIYPTVKPY
  HYPKAGSENPSISLHVIGLNGPTHDLEMMPPDDPRMREYYITMVKWATSTKVAVTWLNRAQNVSILTLCD
  ATTGVCTKKHEDESEAWLHRQNEEPVFSKDGRKFFFIRAIPQGGRGKFYHITVSSSQPNSSNDNIQSITS
  GDWDVTKILAYDEKGNKIYFLSTEDLPRRRQLYSANTVGNFNRQCLSCDLVENCTYFSASFSHSMDFFLL
  KCEGPGVPMVTVHNTTDKKKMFDLETNEHVKKAINDRQMPKVEYRDIEIDDYNLPMQILKPATFTDTTHY
  PLLLVVDGTPGSQSVAEKFEVSWETVMVSSHGAVVVKCDGRGSGFQGTKLLHEVRRRLGLLEEKDQMEAV
  RTMLKEQYIDRTRVAVFGKDYGGYLSTYILPAKGENQGQTFTCGSALSPITDFKLYASAFSERYLGLHGL
  DNRAYEMTKVAHRVSALEEQQFLIIHPTADEKIHFQHTAELITQLIRGKANYSLQIYPDESHYFTSSSLK
  QHLYRSIINFFVECFRIQDKLPTVTAKEDEEED
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001936
• ORF Size: 2409 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001936.2, NP_001927.2
• RefSeq Size: 3560 bp
• RefSeq ORF: 2412 bp
• Locus ID: 1804
• Cytogenetics: 7q36.2
• Domains: Peptidase_S9, DPPIV_N_term
• Protein Families: Druggable Genome, Protease, Transmembrane
• Gene Summary: 'This gene encodes a single-pass type II membrane protein that is a member of the peptidase S9B family of serine proteases. This protein has no detectable protease activity, most likely due to the absence of the conserved serine residue normally present in the catalytic domain of serine proteases. However, it does bind specific voltage-gated potassium channels and alters their expression and biophysical properties. Variations in this gene may be associated with susceptibility to amyotrophic lateral sclerosis and with idiopathic ventricular fibrillation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]'