Separase (ESPL1) (NM_012291) Human Tagged ORF Clone

CAT#: RG217230

ESPL1 (GFP-tagged) - Human extra spindle pole bodies homolog 1 (S. cerevisiae) (ESPL1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ESPL1
• Synonyms: ESP1; SEPA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217230 representing NM_012291
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGAGCTTCAAAAGAGTCAACTTTGGGACTCTGCTAAGCAGCCAGAAGGAGGCTGAAGAGTTGCTGC
  CCGCCTTGAAGGAGTTCCTGTCCAACCCTCCAGCTGGTTTTCCCAGCAGCCGATCTGATGCTGAGAGGAG
  ACAAGCTTGTGATGCCATCCTGAGGGCTTGCAACCAGCAGCTGACTGCTAAGCTAGCTTGCCCTAGGCAT
  CTGGGGAGCCTGCTGGAGCTGGCAGAGCTGGCCTGTGATGGCTACTTAGTGTCTACCCCACAGCGTCCTC
  CCCTCTACCTGGAACGAATTCTCTTTGTCTTACTGCGGAATGCTGCTGCACAAGGAAGCCCAGAGGCCAC
  ACTCCGCCTTGCTCAGCCCCTCCATGCCTGCTTGGTGCAGTGCTCTCGCGAGGCTGCTCCCCAGGACTAT
  GAGGCCGTGGCTCGGGGCAGCTTTTCTCTGCTTTGGAAGGGGGCAGAAGCCCTGTTGGAACGGCGAGCTG
  CATTTGCAGCTCGGCTGAAGGCCTTGAGCTTCCTAGTACTCTTGGAGGATGAAAGTACCCCTTGTGAGGT
  TCCTCACTTTGCTTCTCCAACAGCCTGTCGAGCGGTAGCTGCCCATCAGCTATTTGATGCCAGTGGCCAT
  GGTCTAAATGAAGCAGATGCTGATTTCCTAGATGACCTGCTCTCCAGGCACGTGATCAGAGCCTTGGTGG
  GTGAGAGAGGGAGCTCTTCTGGGCTTCTTTCTCCCCAGAGGGCCCTCTGCCTCTTGGAGCTCACCTTGGA
  ACACTGCCGTCGCTTTTGCTGGAGCCGCCACCATGACAAAGCCATCAGCGCAGTGGAGAAGGCTCACAGT
  TACCTAAGGAACACCAATCTAGCCCCTAGCCTTCAGCTATGTCAGCTGGGGGTTAAGCTGCTGCAGGTTG
  GGGAGGAAGGACCTCAGGCAGTGGCCAAGCTTCTGATCAAGGCATCAGCTGTCCTGAGCAAGAGTATGGA
  GGCACCATCACCCCCACTTCGGGCATTGTATGAGAGCTGCCAGTTCTTCCTTTCAGGCCTGGAACGAGGC
  ACCAAGAGGCGCTATAGACTTGATGCCATTCTGAGCCTCTTTGCTTTTCTTGGAGGGTACTGCTCTCTTC
  TGCAGCAGCTGCGGGATGATGGTGTGTATGGGGGCTCCTCCAAGCAACAGCAGTCTTTTCTTCAGATGTA
  CTTTCAGGGACTTCACCTCTACACTGTGGTGGTTTATGACTTTGCCCAAGGCTGTCAGATAGTTGATTTG
  GCTGACCTGACCCAACTAGTGGACAGTTGTAAATCTACCGTTGTCTGGATGCTGGAGGCCTTAGAGGGCC
  TGTCGGGCCAAGAGCTGACGGACCACATGGGGATGACCGCTTCTTACACCAGTAATTTGGCCTACAGCTT
  CTATAGTCACAAGCTCTATGCCGAGGCCTGTGCCATCTCTGAGCCGCTCTGTCAGCACCTGGGTTTGGTG
  AAGCCAGGCACTTATCCCGAGGTGCCTCCTGAGAAGTTGCACAGGTGCTTCCGGCTACAAGTAGAGAGTT
  TGAAGAAACTGGGTAAACAGGCCCAGGGCTGCAAGATGGTGATTTTGTGGCTGGCAGCCCTGCAACCCTG
  TAGCCCTGAACACATGGCTGAGCCAGTCACTTTCTGGGTTCGGGTCAAGATGGATGCGGCCAGGGCTGGA
  GACAAGGAGCTACAGCTAAAGACTCTGCGAGACAGCCTCAGTGGCTGGGACCCGGAGACCCTGGCCCTCC
  TGCTGAGGGAGGAGCTGCAGGCCTACAAGGCGGTGCGGGCCGACACTGGACAGGAACGCTTCAACATCAT
  CTGTGACCTCCTGGAGCTGAGCCCCGAGGAGACACCAGCCGGGGCCTGGGCACGAGCCACCCACCTGGTA
  GAACTGGCTCAGGTGCTCTGCTACCACGACTTTACGCAGCAGACCAACTGCTCTGCTCTGGATGCTATCC
  GGGAAGCCCTGCAGCTTCTGGACTCTGTGAGGCCTGAGGCCCAGGCCAGAGATCAGCTTCTGGACGATAA
  AGCACAGGCCTTGCTGTGGCTTTACATCTGTACTCTGGAAGCCAAAATGCAGGAAGGTATCGAGCGGGAT
  CGGAGAGCCCAGGCCCCTGGTAACTTGGAGGAATTTGAAGTCAATGACCTGAACTATGAAGATAAACTCC
  AGGAAGATCGTTTCCTATACAGTAACATTGCCTTCAACCTGGCTGCAGATGCTGCTCAGTCCAAATGCCT
  GGACCAAGCCCTGGCCCTGTGGAAGGAGCTGCTTACAAAGGGGCAGGCCCCAGCTGTACGGTGTCTCCAG
  CAGACAGCAGCCTCACTGCAGATCCTAGCAGCCCTCTACCAGCTGGTGGCAAAGCCCATGCAGGCTCTGG
  AGGTCCTCCTGCTGCTACGGATTGTCTCTGAGAGACTGAAGGACCACTCGAAGGCAGCTGGCTCCTCCTG
  CCACATCACCCAGCTCCTCCTGACCCTCGGCTGTCCCAGCTATGCCCAGTTACACCTGGAAGAGGCAGCA
  TCGAGCCTGAAGCATCTCGATCAGACTACTGACACATACCTGCTCCTTTCCCTGACCTGTGATCTGCTTC
  GAAGTCAACTCTACTGGACTCACCAGAAGGTGACCAAGGGTGTCTCTCTGCTGCTGTCTGTGCTTCGGGA
  TCCTGCCCTCCAGAAGTCCTCCAAGGCTTGGTACTTGCTGCGTGTCCAGGTCCTGCAGCTGGTGGCAGCT
  TACCTTAGCCTCCCGTCAAACAACCTCTCACACTCCCTGTGGGAGCAGCTCTGTGCCCAAGGCTGGCAGA
  CACCTGAGATAGCTCTCATAGACTCCCATAAGCTCCTCCGAAGCATCATCCTCCTGCTGATGGGCAGTGA
  CATTCTCTCAACTCAGAAAGCAGCTGTGGAGACATCGTTTTTGGACTATGGTGAAAATCTGGTACAAAAA
  TGGCAGGTTCTTTCAGAGGTGCTGAGCTGCTCAGAGAAGCTGGTCTGCCACCTGGGCCGCCTGGGTAGTG
  TGAGTGAAGCCAAGGCCTTTTGCTTGGAGGCCCTAAAACTTACAACAAAGCTGCAGATACCACGCCAGTG
  TGCCCTGTTCCTGGTGCTGAAGGGCGAGCTGGAGCTGGCCCGCAATGACATTGATCTCTGTCAGTCGGAC
  CTGCAGCAGGTTCTGTTCTTGCTTGAGTCTTGCACAGAGTTTGGTGGGGTGACTCAGCACCTGGACTCTG
  TGAAGAAGGTCCACCTGCAGAAGGGGAAGCAGCAGGCCCAGGTCCCCTGTCCTCCACAGCTCCCAGAGGA
  GGAGCTCTTCCTAAGAGGCCCTGCTCTAGAGCTGGTGGCCACTGTGGCCAAGGAGCCTGGCCCCATAGCA
  CCTTCTACAAACTCCTCCCCAGTCTTGAAAACCAAGCCCCAGCCCATACCCAACTTCCTGTCCCATTCAC
  CCACCTGTGACTGCTCGCTCTGCGCCAGCCCTGTCCTCACAGCAGTCTGTCTGCGCTGGGTATTGGTCAC
  GGCAGGGGTGAGGCTGGCCATGGGCCACCAAGCCCAGGGTCTGGATCTGCTGCAGGTCGTGCTGAAGGGC
  TGTCCTGAAGCCGCTGAGCGCCTCACCCAAGCTCTCCAAGCTTCCCTGAATCATAAAACACCCCCCTCCT
  TGGTTCCAAGCCTCTTGGATGAGATCTTGGCTCAAGCATACACACTGTTGGCACTGGAGGGCCTGAACCA
  GCCATCAAACGAGAGCCTGCAGAAGGTTCTACAGTCAGGGCTGAAGTTTGTAGCAGCACGGATACCCCAC
  CTAGAGCCCTGGCGAGCCAGCCTGCTCTTGATTTGGGCCCTCACAAAACTAGGTGGCCTCAGCTGCTGTA
  CTACCCAACTTTTTGCAAGCTCCTGGGGCTGGCAGCCACCATTAATAAAAAGTGTCCCTGGCTCAGAGCC
  CTCTAAGACTCAGGGCCAAAAACGTTCTGGACGAGGGCGCCAAAAGTTAGCCTCTGCTCCCCTGCGCCTC
  AATAATACCTCTCAGAAAGGTCTGGAAGGTAGAGGACTGCCCTGCACACCTAAACCCCCAGACCGGATCA
  GGCAAGCTGGCCCTCATGTCCCCTTCACGGTGTTTGAGGAAGTCTGCCCTACAGAGAGCAAGCCTGAAGT
  ACCCCAGGCCCCCAGGGTACAACAGAGAGTCCAGACGCGCCTCAAGGTGAACTTCAGTGATGACAGTGAC
  TTGGAAGACCCTGTCTCAGCTGAGGCCTGGCTGGCAGAGGAGCCTAAGAGACGGGGCACTGCTTCCCGGG
  GCCGGGGGCGAGCAAGGAAGGGCCTGAGCCTAAAGACGGATGCCGTGGTTGCCCCAGGTAGTGCCCCTGG
  GAACCCTGGCCTGAATGGCAGGAGCCGGAGGGCCAAGAAGGTGGCATCAAGACATTGTGAGGAGCGGCGT
  CCCCAGAGGGCCAGTGACCAGGCCAGGCCTGGCCCTGAGATCATGAGGACCATCCCTGAGGAAGAACTGA
  CTGACAACTGGAGAAAAATGAGCTTTGAGATCCTCAGGGGCTCTGACGGGGAAGACTCAGCCTCAGGTGG
  GAAGACTCCAGCTCCGGGCCCTGAGGCAGCTTCTGGAGAATGGGAGCTGCTGAGGCTGGATTCCAGCAAG
  AAGAAGCTGCCCAGCCCATGCCCAGACAAGGAGAGTGACAAGGACCTTGGTCCTCGGCTCCGGCTCCCCT
  CAGCCCCCGTAGCCACTGGTCTTTCTACCCTGGACTCCATCTGTGACTCCCTGAGTGTTGCTTTCCGGGG
  CATTAGTCACTGTCCTCCTAGTGGGCTCTATGCCCACCTCTGCCGCTTCCTGGCCTTGTGCCTGGGCCAC
  CGGGATCCTTATGCCACTGCTTTCCTTGTCACCGAGTCTGTCTCCATCACCTGTCGCCACCAGCTGCTCA
  CCCACCTCCACAGACAGCTCAGCAAGGCCCAGAAGCACCGAGGATCACTTGAAATAGCAGACCAGCTGCA
  GGGGCTGAGCCTTCAGGAGATGCCTGGAGATGTCCCCCTGGCCCGCATCCAGCGCCTCTTTTCCTTCAGG
  GCTTTGGAATCTGGCCACTTCCCCCAGCCTGAAAAGGAGAGTTTCCAGGAGCGCCTGGCTCTGATCCCCA
  GTGGGGTGACTGTGTGTGTGTTGGCCCTGGCCACCCTCCAGCCCGGAACCGTGGGCAACACCCTCCTGCT
  GACCCGGCTGGAAAAGGACAGTCCCCCAGTCAGTGTGCAGATTCCCACTGGCCAGAACAAGCTTCATCTG
  CGTTCAGTCCTGAATGAGTTTGATGCCATCCAGAAGGCACAGAAAGAGAACAGCAGCTGTACTGACAAGC
  GAGAATGGTGGACAGGGCGGCTGGCACTGGACCACAGGATGGAGGTTCTCATCGCTTCCCTAGAGAAGTC
  TGTGCTGGGCTGCTGGAAGGGGCTGCTGCTGCCGTCCAGTGAGGAGCCCGGCCCTGCCCAGGAGGCCTCC
  CGCCTACAGGAGCTGCTACAGGACTGTGGCTGGAAATATCCTGACCGCACTCTGCTGAAAATCATGCTCA
  GTGGTGCCGGTGCCCTCACCCCTCAGGACATTCAGGCCCTGGCCTACGGGCTGTGCCCAACCCAGCCAGA
  GCGAGCCCAGGAGCTCCTGAATGAGGCAGTAGGACGTCTACAGGGCCTGACAGTACCAAGCAATAGCCAC
  CTTGTCTTGGTCCTAGACAAGGACTTGCAGAAGCTGCCGTGGGAAAGCATGCCCAGCCTCCAAGCACTGC
  CTGTCACCCGGCTGCCCTCCTTCCGCTTCCTACTCAGCTACTCCATCATCAAAGAGTATGGGGCCTCGCC
  AGTGCTGAGTCAAGGGGTGGATCCACGAAGTACCTTCTATGTCCTGAACCCTCACAATAACCTGTCAAGC
  ACAGAGGAGCAATTTCGAGCCAATTTCAGCAGTGAAGCTGGCTGGAGAGGAGTGGTTGGGGAGGTGCCAA
  GACCTGAACAGGTGCAGGAAGCCCTGACAAAGCATGATTTGTATATCTATGCAGGGCATGGGGCTGGTGC
  CCGCTTCCTTGATGGGCAGGCTGTCCTGCGGCTGAGCTGTCGGGCAGTGGCCCTGCTGTTTGGCTGTAGC
  AGTGCGGCCCTGGCTGTGCGTGGAAACCTGGAGGGGGCTGGCATCGTGCTCAAGTACATCATGGCTGGTT
  GCCCCTTGTTTCTGGGTAATCTCTGGGATGTGACTGACCGCGACATTGACCGCTACACGGAAGCTCTGCT
  GCAAGGCTGGCTTGGAGCAGGCCCAGGGGCCCCCCTTCTCTACTATGTAAACCAGGCCCGCCAAGCTCCC
  CGACTCAAGTATCTTATTGGGGCTGCACCTATAGCCTATGGCTTGCCTGTCTCTCTGCGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG217230 representing NM_012291
  Red=Cloning site Green=Tags(s)
  
  MRSFKRVNFGTLLSSQKEAEELLPALKEFLSNPPAGFPSSRSDAERRQACDAILRACNQQLTAKLACPRH
  LGSLLELAELACDGYLVSTPQRPPLYLERILFVLLRNAAAQGSPEATLRLAQPLHACLVQCSREAAPQDY
  EAVARGSFSLLWKGAEALLERRAAFAARLKALSFLVLLEDESTPCEVPHFASPTACRAVAAHQLFDASGH
  GLNEADADFLDDLLSRHVIRALVGERGSSSGLLSPQRALCLLELTLEHCRRFCWSRHHDKAISAVEKAHS
  YLRNTNLAPSLQLCQLGVKLLQVGEEGPQAVAKLLIKASAVLSKSMEAPSPPLRALYESCQFFLSGLERG
  TKRRYRLDAILSLFAFLGGYCSLLQQLRDDGVYGGSSKQQQSFLQMYFQGLHLYTVVVYDFAQGCQIVDL
  ADLTQLVDSCKSTVVWMLEALEGLSGQELTDHMGMTASYTSNLAYSFYSHKLYAEACAISEPLCQHLGLV
  KPGTYPEVPPEKLHRCFRLQVESLKKLGKQAQGCKMVILWLAALQPCSPEHMAEPVTFWVRVKMDAARAG
  DKELQLKTLRDSLSGWDPETLALLLREELQAYKAVRADTGQERFNIICDLLELSPEETPAGAWARATHLV
  ELAQVLCYHDFTQQTNCSALDAIREALQLLDSVRPEAQARDQLLDDKAQALLWLYICTLEAKMQEGIERD
  RRAQAPGNLEEFEVNDLNYEDKLQEDRFLYSNIAFNLAADAAQSKCLDQALALWKELLTKGQAPAVRCLQ
  QTAASLQILAALYQLVAKPMQALEVLLLLRIVSERLKDHSKAAGSSCHITQLLLTLGCPSYAQLHLEEAA
  SSLKHLDQTTDTYLLLSLTCDLLRSQLYWTHQKVTKGVSLLLSVLRDPALQKSSKAWYLLRVQVLQLVAA
  YLSLPSNNLSHSLWEQLCAQGWQTPEIALIDSHKLLRSIILLLMGSDILSTQKAAVETSFLDYGENLVQK
  WQVLSEVLSCSEKLVCHLGRLGSVSEAKAFCLEALKLTTKLQIPRQCALFLVLKGELELARNDIDLCQSD
  LQQVLFLLESCTEFGGVTQHLDSVKKVHLQKGKQQAQVPCPPQLPEEELFLRGPALELVATVAKEPGPIA
  PSTNSSPVLKTKPQPIPNFLSHSPTCDCSLCASPVLTAVCLRWVLVTAGVRLAMGHQAQGLDLLQVVLKG
  CPEAAERLTQALQASLNHKTPPSLVPSLLDEILAQAYTLLALEGLNQPSNESLQKVLQSGLKFVAARIPH
  LEPWRASLLLIWALTKLGGLSCCTTQLFASSWGWQPPLIKSVPGSEPSKTQGQKRSGRGRQKLASAPLRL
  NNTSQKGLEGRGLPCTPKPPDRIRQAGPHVPFTVFEEVCPTESKPEVPQAPRVQQRVQTRLKVNFSDDSD
  LEDPVSAEAWLAEEPKRRGTASRGRGRARKGLSLKTDAVVAPGSAPGNPGLNGRSRRAKKVASRHCEERR
  PQRASDQARPGPEIMRTIPEEELTDNWRKMSFEILRGSDGEDSASGGKTPAPGPEAASGEWELLRLDSSK
  KKLPSPCPDKESDKDLGPRLRLPSAPVATGLSTLDSICDSLSVAFRGISHCPPSGLYAHLCRFLALCLGH
  RDPYATAFLVTESVSITCRHQLLTHLHRQLSKAQKHRGSLEIADQLQGLSLQEMPGDVPLARIQRLFSFR
  ALESGHFPQPEKESFQERLALIPSGVTVCVLALATLQPGTVGNTLLLTRLEKDSPPVSVQIPTGQNKLHL
  RSVLNEFDAIQKAQKENSSCTDKREWWTGRLALDHRMEVLIASLEKSVLGCWKGLLLPSSEEPGPAQEAS
  RLQELLQDCGWKYPDRTLLKIMLSGAGALTPQDIQALAYGLCPTQPERAQELLNEAVGRLQGLTVPSNSH
  LVLVLDKDLQKLPWESMPSLQALPVTRLPSFRFLLSYSIIKEYGASPVLSQGVDPRSTFYVLNPHNNLSS
  TEEQFRANFSSEAGWRGVVGEVPRPEQVQEALTKHDLYIYAGHGAGARFLDGQAVLRLSCRAVALLFGCS
  SAALAVRGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLGAGPGAPLLYYVNQARQAP
  RLKYLIGAAPIAYGLPVSLR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012291
• ORF Size: 6360 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012291.4, NP_036423.4
• RefSeq Size: 6641
• RefSeq ORF: 6363
• Locus ID: 9700
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Cell cycle, Oocyte meiosis
• Gene Summary: Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]). [supplied by OMIM, Nov 2010]