Separase (ESPL1) (NM_012291) Human Untagged Clone

CAT#: SC125552

ESPL1 (untagged)-Human extra spindle pole bodies homolog 1 (S. cerevisiae) (ESPL1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ESPL1
• Synonyms: ESP1; SEPA
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125552 sequence for NM_012291 edited (data generated by NextGen Sequencing)
  ATGAGGAGCTTCAAAAGAGTCAACTTTGGGACTCTGCTAAGCAGCCAGAAGGAGGCTGAA
  GAGTTGCTGCCCGACTTGAAGGAGTTCCTGTCCAACCCTCCAGCTGGTTTTCCCAGCAGC
  CGATCTGATGCTGAGAGGAGACAAGCTTGTGATGCCATCCTGAGGGCTTGCAACCAGCAG
  CTGACTGCTAAGCTAGCTTGCCCTAGGCATCTGGGGAGCCTGCTGGAGCTGGCAGAGCTG
  GCCTGTGATGGCTACTTAGTGTCTACCCCACAGCGTCCTCCCCTCTACCTGGAACGAATT
  CTCTTTGTCTTACTGCGGAATGCTGCTGCACAAGGAAGCCCAGAGGCCACACTCCGCCTT
  GCTCAGCCCCTCCATGCCTGCTTGGTGCAGTGCTCTCGCGAGGCTGCTCCCCAGGACTAT
  GAGGCCGTGGCTCGGGGCAGCTTTTCTCTGCTTTGGAAGGGGGCAGAAGCCCTGTTGGAA
  CGGCGAGCTGCATTTGCAGCTCGGCTGAAGGCCTTGAGCTTCCTAGTACTCTTGGAGGAT
  GAAAGTACCCCTTGTGAGGTTCCTCACTTTGCTTCTCCAACAGCCTGTCGAGCGGTAGCT
  GCCCATCAGCTATTTGATGCCAGTGGCCATGGTCTAAATGAAGCAGATGCTGATTTCCTA
  GATGACCTGCTCTCCAGGCACGTGATCAGAGCCTTGGTGGGTGAGAGAGGGAGCTCTTCT
  GGGCTTCTTTCTCCCCAGAGGGCCCTCTGCCTCTTGGAGCTCACCTTGGAACACTGCCGT
  CGCTTTTGCTGGAGCCGCCACCATGACAAAGCCATCAGCGCAGTGGAGAAGGCTCACAGT
  TACCTAAGGAACACCAATCTAGCCCCTAGCCTTCAGCTATGTCAGCTGGGGGTTAAGCTG
  CTGCAGGTTGGGGAGGAAGGACCTCAGGCAGTGGCCAAGCTTCTGATCAAGGCATCAGCT
  GTCCTGAGCAAGAGTATGGAGGCACCATCACCCCCACTTCGGGCATTGTATGAGAGCTGC
  CAGTTCTTCCTTTCAGGCCTGGAACGAGGCACCAAGAGGCGCTATAGACTTGATGCCATT
  CTGAGCCTCTTTGCTTTTCTTGGAGGGTACTGCTCTCTTCTGCAGCAGCTGCGGGATGAT
  GGTGTGTATGGGGGCTCCTCCAAGCAACAGCAGTCTTTTCTTCAGATGTACTTTCAGGGA
  CTTCACCTCTACACTGTGGTGGTTTATGACTTTGCCCAAGGCTGTCAGATAGTTGATTTG
  GCTGACCTGACCCAACTAGTGGACAGTTGTAAATCTACCGTTGTCTGGATGCTGGAGGCC
  TTAGAGGGCCTGTCGGGCCAAGAGCTGACGGACCACATGGGGATGACCGCTTCTTACACC
  AGTAATTTGGCCTACAGCTTCTATAGTCACAAGCTCTATGCCGAGGCCTGTGCCATCTCT
  GAGCCGCTCTGTCAGCACCTGGGTTTGGTGAAGCCAGGCACTTATCCCGAGGTGCCTCCT
  GAGAAGTTGCACAGGTGCTTCCGGCTACAAGTAGAGAGTTTGAAGAAACTGGGTAAACAG
  GCCCAGGGCTGCAAGATGGTGATTTTGTGGCTGGCAGCCCTGCAACCCTGTAGCCCTGAA
  CACATGGCTGAGCCAGTCACTTTCTGGGTTCGGGTCAAGATGGATGCGGCCAGGGCTGGA
  GACAAGGAGCTACAGCTAAAGACTCTGCGAGACAGCCTCAGTGGCTGGGACCCGGAGACC
  CTGGCCCTCCTGCTGAGGGAGGAGCTGCAGGCCTACAAGGCGGTGCGGGCCGACACTGGA
  CAGGAACGCTTCAACATCATCTGTGACCTCCTGGAGCTGAGACCCGAGGAGACACCAGCC
  GGGGCCTGGGCACGAGCCACCCACCTGGTAGAACTGGCTCAGGTGCTCTGCTACCACGAC
  TTTACGCAGCAGACCAACTGCTCTGCTCTGGATGCTATCCGGGAAGCCCTGCAGCTTCTG
  GACTCTGTGAGGCCTGAGGCCCAGGCCAGAGATCAGCTTCTGGACGATAAAGCACAGGCC
  TTGCTGTGGCTTTACATCTGTACTCTGGAAGCCAAAATGCAGGAAGGTATCGAGCGGGAT
  CGGAGAGCCCAGGCCCCTGGTAACTTGGAGGAATTTGAAGTCAATGACCTGAACTATGAA
  GATAAACTCCAGGAAGATCGTTTCCTATACAGTAACATTGCCTTCAACCTGGCTGCAGAT
  GCTGCTCAGTCCAAATGCCTGGACCAAGCCCTGGCCCTGTGGAAGGAGCTGCTTACAAAG
  GGGCAGGCCCCAGCTGTACGGTGTCTCCAGCAGACAGCAGCCTCACTGCAGATCCTAGCA
  GCCCTCTACCAGCTGGTGGCAAAGCCCATGCAGGCTCTGGAGGTCCTCCTGCTGCTACGG
  ATTGTCTCTGAGAGACTGAAGGACCACTCGAAGGCAGCTGGCTCCTCCTGCCACATCACC
  CAGCTCCTCCTGACCCTCGGCTGTCCCAGCTATGCCCAGTTACACCTGGAAGAGGCAGCA
  TCGAGCCTGAAGCATCTCGATCAGACTACTGACACATACCTGCTCCTTTCCCTGACCTGT
  GATCTGCTTCGAAGTCAACTCTACTGGACTCACCAGAAGGTGACCAAGGGTGTCTCTCTG
  CTGCTGTCTGTGCTTCGGGATCCTGCCCTCCAGAAGTCCTCCAAGGCTTGGTACTTGCTG
  CGTGTCCAGGTCCTGCAGCTGGTGGCAGCTTACCTTAGCCTCCCGTCAAACAACCTCTCA
  CACTCCCTGTGGGAGCAGCTCTGTGCCCAAGGCTGGCAGACACCTGAGATAGCTCTCATA
  GACTCCCATAAGCTCCTCCGAAGCATCATCCTCCTGCTGATGGGCAGTGACATTCTCTCA
  ACTCAGAAAGCAGCTGTGGAGACATCGTTTTTGGACTATGGTGAAAATCTGGTACAAAAA
  TGGCAGGTTCTTTCAGAGGTGCTGAGCTGCTCAGAGAAGCTGGTCTGCCACCTGGGCCGC
  CTGGGTAGTGTGAGTGAAGCCAAGGCCTTTTGCTTGGAGGCCCTAAAACTTACAACAAAG
  CTGCAGATACCACGCCAGTGTGCCCTGTTCCTGGTGCTGAAGGGCGAGCTGGAGCTGGCC
  CGCAATGACATTGATCTCTGTCAGTCGGACCTGCAGCAGGTTCTGTTCTTGCTTGAGTCT
  TGCACAGAGTTTGGTGGGGTGACTCAGCACCTGGACTCTGTGAAGAAGGTCCACCTGCAG
  AAGGGGAAGCAGCAGGCCCAGGTCCCCTGTCCTCCACAGCTCCCAGAGGAGGAGCTCTTC
  CTAAGAGGCCCTGCTCTAGAGCTGGTGGCCACTGTGGCCAAGGAGCCTGGCCCCATAGCA
  CCTTCTACAAACTCCTCCCCAGTCTTGAAAACCAAGCCCCAGCCCATACCCAACTTCCTG
  TCCCATTCACCCACCTGTGACTGCTCGCTCTGCGCCAGCCCTGTCCTCACAGCAGTCTGT
  CTGCGCTGGGTATTGGTCACGGCAGGGGTGAGGCTGGCCATGGGCCACCAAGCCCAGGGT
  CTGGATCTGCTGCAGGTCGTGCTGAAGGGCTGTCCTGAAGCCGCTGAGCGCCTCACCCAA
  GCTCTCCAAGCTTCCCTGAATCATAAAACACCCCCCTCCTTGGTTCCAAGCCTCTTGGAT
  GAGATCTTGGCTCAAGCATACACACTGTTGGCACTGGAGGGCCTGAACCAGCCATCAAAC
  GAGAGCCTGCAGAAGGTTCTACAGTCAGGGCTGAAGTTTGTAGCAGCACGGATACCCCAC
  CTAGAGCCCTGGCGAGCCAGCCTGCTCTTGATTTGGGCCCTCACAAAACTAGGTGGCCTC
  AGCTGCTGTACTACCCAACTTTTTGCAAGCTCCTGGGGCTGGCAGCCACCATTAATAAAA
  AGTGTCCCTGGCTCAGAGCCCTCTAAGACTCAGGGCCAAAAACGTTCTGGACGAGGGCGC
  CAAAAGTTAGCCTCTGCTCCCCTGCGCCTCAATAATACCTCTCAGAAAGGTCTGGAAGGT
  AGAGGACTGCCCTGCACACCTAAACCCCCAGACCGGATCAGGCAAGCTGGCCCTCATGTC
  CCCTTCACGGTGTTTGAGGAAGTCTGCCCTACAGAGAGCAAGCCTGAAGTACCCCAGGCC
  CCCAGGGTACAACAGAGAGTCCAGACGCGCCTCAAGGTGAACTTCAGTGATGACAGTGAC
  TTGGAAGACCCTGTCTCAGCTGAGGCCTGGCTGGCAGAGGAGCCTAAGAGACGGGGCACT
  GCTTCCCGGGGCCGGGGGCGAGCAAGGAAGGGCCTGAGCCTAATGACGGATGCCGTGGTT
  GCCCCAGGTAGTGCCCCTGGGAACCCTGGCCTGAATGGCAGGAGCCGGAGGGCCAAGAAG
  GTGGCATCAAGACATTGTGAGGAGCGGCGTCCCCAGAGGGCCAGTGACCAGGCCAGGCCT
  GGCCCTGAGATCATGAGGACCATCCCTGAGGAAGAACTGACTGACAACTGGAGAAAAATG
  AGCTTTGAGATCCTCAGGGGCTCTGACGGGGAAGACTCAGCCTCAGGTGGAAAGACTCCA
  GCTCCGGGCCCTGAGGCAGCTTCTGGAGAATGGGAGCTGCTGAGGCTGGATTCCAGCAAG
  AAGAAGCTGCCCAGCCCATGCCCAGACAAGGAGAGTGACAAGGACCTTGGTCCTCGGCTC
  CGGCTCCCCTCAGCCCCCGTAGCCACTGGTCTTTCTACCCTGGACTCCATCTGTGACTCC
  CTGAGTGTTGCTTTCCGGGGCATTAGTCACTGTCCTCCTAGTGGGCTCTATGCCCACCTC
  TGCCGCTTCCTGGCCTTGTGCCTGGGCCACCGGGATCCTTATGCCACTGCTTTCCTTGTC
  ACCGAGTCTGTCTCCATCACCTGTCGCCACCAGCTGCTCACCCACCTCCACAGACAGCTC
  AGCAAGGCCCAGAAGCACCGAGGATCACTTGAAATAGCAGACCAGCTGCAGGGGCTGAGC
  CTTCAGGAGATGCCTGGAGATGTCCCCCTGGCCCGCATCCAGCGCCTCTTTTCCTTCAGG
  GCTTTGGAATCTGGCCACTTCCCCCAGCCTGAAAAGGAGAGTTTCCAGGAGCGCCTGGCT
  CTGATCCCCAGTGGGGTGACTGTGTGTGTGTTGGCCCTGGCCACCCTCCAGCCCGGAACC
  GTGGGCAACACCCTCCTGCTGACCCGGCTGGAAAAGGACAGTCCCCCAGTCAGTGTGCAG
  ATTCCCACTGGCCAGAACAAGCTTCATCTGCGTTCAGTCCTGAATGAGTTTGATGCCATC
  CAGAAGGCACAGAAAGAGAACAGCAGCTGTACTGACAAGCGAGAATGGTGGACAGGGCGG
  CTGGCACTGGACCACAGGATGGAGGTTCTCATCGCTTCCCTAGAGAAGTCTGTGCTGGGC
  TGCTGGAAGGGGCTGCTGCTGCCGTCCAGTGAGGAGCCCGGCCCTGCCCAGGAGGCCTCC
  CGCCTACAGGAGCTGCTACAGGACTGTGGCTGGAAATATCCTGACCGCACTCTGCTGAAA
  ATCATGCTCAGTGGTGCCGGTGCCCTCACCCCTCAGGACATTCAGGCCCTGGCCTACGGG
  CTGTGCCCAACCCAGCCAGAGCGAGCCCAGGAGCTCCTGAATGAGGCAGTAGGACGTCTA
  CAGGGCCTGACAGTACCAAGCAATAGCCACCTTGTCTTGGTCCTAGACAAGGACTTGCAG
  AAGCTGCCGTGGGAAAGCATGCCCAGCCTCCAAGCACTGCCTGTCACCCGGCTGCCCTCC
  TTCCGCTTCCTACTCAGCTACTCCATCATCAAAGAGTATGGGGCCTCGCCAGTGCTGAGT
  CAAGGGGTGGATCCACGAAGTACCTTCTATGTCCTGAACCCTCACAATAACCTGTCAAGC
  ACAGAGGAGCAATTTCGAGCCAATTTCAGCAGTGAAGCTGGCTGGAGAGGAGTGGTTGGG
  GAGGTGCCAAGACCTGAACAGGTGCAGGAAGCCCTGACAAAGCATGATTTGTATATCTAT
  GCAGGGCATGGGGCTGGTGCCCGCTTCCTTGATGGGCAGGCTGTCCTGCGGCTGAGCTGT
  CGGGCAGTGGCCCTGCTGTTTGGCTGTAGCAGTGCGGCCCTGGCTGTGCATGGAAACCTG
  GAGGGGGCTGGCATCGTGCTCAAGTACATCATGGCTGGTTGCCCCTTGTTTCTGGGTAAT
  CTCTGGGATGTGACTGACCGCGACATTGACCGCTACACGGAAGCTCTGCTGCAAGGCTGG
  CTTGGAGCAGGCCCAGGGGCCCCCCTTCTCTACTATGTAAACCAGGCCCGCCAAGCTCCC
  CGACTCAAGTATCTTATTGGGGCTGCACCTATAGCCTATGGCTTGCCTGTCTCTCTGCGG
  TAA
  
  Clone variation with respect to NM_012291.4
  74 c=>a;1842 c=>a;4304 a=>t;4551 g=>a;6110 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012291 unedited
  NNCCGTCTTTCCCCCGCCCGTTGNCGCATAGGGCGGTAGGCGTGTACGGTGGGAGGTCTA
  TATAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGC
  GGCCGCGAATTCGGCACGAGGGGAGTACTGGTCAGGCGGTTAAGTCCTGTACCTAGGAAA
  GAGGGCGAGCTCTGGGGCGGTAAGGCCGGAAGGGACTCGTGAGCGTGGCTCTCCGGTGTC
  ATGAGGAGCTTCAAAAGAGTCAACTTTGGGACTCTGCTAAGCAGCCAGAAGGAGGCTGAA
  GAGTTGCTGCCCGACTTGAAGGAGTTCCTGTCCAACCCTCCAGCTGGTTTTCCCAGCAGC
  CGATCTGATGCTGAGAGGAGACAAGCTTGTGATGCCATCCTGAGGGCTTGCAACCAGCAG
  CTGACTGCTAAGCTAGCTTGCCCTANGCATCTGGNGAGCCTGCTGGAGCTGGCAGAGCTG
  GCCTGTGATGGCTACTTAGTGTCTACCCCACAGCGTCCTCCCCTCTACCTGGAACGAATT
  CTCTTTGTCTTACTGGCGAATGCTGCTGCACAGGAAGCCCCAGAGCACACNTCGNCTTGG
  CTCAGCCCTNCATGCCTGCTTGGTGCAGTGCTCTCGCGAGGCTGCNTCCCAGGACTATGA
  GGCCGTGGGCTCGGGCAGCTTTTTCTCTGCTTTTGAAGGGNGGCAGAAGCCCTGGTGGAA
  ACGCAAGCTGCATTTGCAGCTNCGCTGAAGGCCTTGAGCTTCTAGTACTCTTGGAGATGA
  AAATACCCCTTGGGAGGTTCCTTACTTTGCTTTCTCACAGCTTGTCAAGCGGTAGCTGCC
  CATCAGCTATTTGATGCCAGTGGCCATGGTCTAAATGAAGCANATGCTGATTTNCTAGAT
  GN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_012291
• ORF Size: 6363 bp
• Insert Size: 7000
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_012291.3, NP_036423.3
• RefSeq Size: 6552
• RefSeq ORF: 6363
• Locus ID: 9700
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Cell cycle, Oocyte meiosis
• Gene Summary: Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]). [supplied by OMIM, Nov 2010]