ALDH5A1 (NM_170740) Human Tagged ORF Clone

CAT#: RG217303

ALDH5A1 (GFP-tagged) - Human aldehyde dehydrogenase 5 family, member A1 (ALDH5A1), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ALDH5A1
• Synonyms: SSADH; SSDH
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217303 representing NM_170740
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACCTGCATTTGGCTGCGGAGCTGTGGGGCCCGGCGCCTCGGGTCGACGTTTCCAGGCTGCCGCC
  TCCGCCCCCGCGCCGGCGGCCTGGTCCCTGCCTCCGGGCCTGCGCCCGGCCCGGCCCAGCTCCGCTGCTA
  CGCTGGGCGCCTGGCGGGCCTCTCTGCGGCGCTGCTGCGCACCGACAGCTTCGTGGGCGGCCGCTGGCTC
  CCGGCCGCCGCCACCTTCCCCGTGCAAGACCCGGCCAGCGGCGCCGCTCTGGGCATGGTAGCCGACTGCG
  GGGTGCGAGAGGCCCGCGCCGCCGTGCGCGCTGCCTACGAGGCTTTCTGCCGCTGGAGGGAGGTCTCCGC
  CAAGGAGAGGAGTTCATTACTTCGGAAGTGGTACAATTTAATGATACAAAATAAGGATGACCTTGCCAGA
  ATAATCACAGCTGAAAGTGGAAAGCCACTGAAGGAGGCACATGGAGAAATTCTCTATTCCGCCTTTTTCC
  TAGAGTGGTTCTCTGAGGAAGCCCGCCGTGTTTACGGAGACATTATCCACACCCCGGCAAAGGACAGGCG
  GGCCCTGGTCCTCAAGCAGCCCATAGGCGTGGCTGCAGTCATCACCCCGTGGAATTTCCCCAGTGCCATG
  ATCACCCGGAAGGTGGGGGCCGCCCTGGCAGCCGGCTGTACTGTCGTGGTGAAGCCTGCCGAAGACACGC
  CCTTCTCCGCCCTGGCCCTGGCTGAGGTAAATCAGGGATTTCTTCTTGATTTGGATCCATTGTTGCTTGC
  AAGCCAGGCTGGGATTCCTTCAGGTGTATACAATGTTATTCCCTGTTCTCGAAAGAATGCCAAGGAAGTA
  GGGGAGGCAATTTGTACTGATCCTCTGGTGTCCAAAATTTCCTTTACTGGTTCAACAACTACAGGAAAGA
  TCCTGTTGCACCACGCAGCAAACTCTGTGAAAAGGGTCTCTATGGAGCTGGGCGGCCTTGCTCCATTTAT
  AGTATTTGACAGTGCCAACGTGGACCAGGCTGTAGCAGGGGCCATGGCATCTAAATTTAGGAACACTGGA
  CAGACTTGTGTTTGCTCAAACCAATTCTTGGTGCAAAGGGGCATCCATGATGCCTTTGTAAAAGCATTCG
  CCGAGGCCATGAAGAAGAACCTGCGCGTAGGTAATGGATTTGAGGAAGGAACTACTCAGGGCCCATTAAT
  TAATGAAAAAGCGGTAGAAAAGGTGGAGAAACAGGTGAATGATGCCGTTTCTAAAGGTGCCACCGTTGTG
  ACAGGTGGAAAACGACACCAACTTGGAAAAAATTTCTTTGAGCCTACCCTGCTGTGCAATGTCACCCAGG
  ACATGCTGTGCACTCATGAAGAGACTTTCGGGCCTCTGGCACCAGTTATCAAGTTCGATACAGAGGAGGA
  GGCTATAGCAATCGCTAACGCAGCTGATGTTGGGTTAGCAGGTTATTTTTACTCTCAAGACCCAGCCCAG
  ATCTGGAGAGTGGCAGAGCAGCTGGAAGTGGGCATGGTTGGCGTCAACGAAGGATTAATTTCCTCTGTGG
  AGTGCCCTTTTGGTGGAGTGAAGCAGTCCGGCCTTGGGCGAGAGGGGTCCAAGTATGGCATTGATGAGTA
  TCTGGAACTCAAGTATGTGTGTTACGGGGGCTTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG217303 representing NM_170740
  Red=Cloning site Green=Tags(s)
  
  MATCIWLRSCGARRLGSTFPGCRLRPRAGGLVPASGPAPGPAQLRCYAGRLAGLSAALLRTDSFVGGRWL
  PAAATFPVQDPASGAALGMVADCGVREARAAVRAAYEAFCRWREVSAKERSSLLRKWYNLMIQNKDDLAR
  IITAESGKPLKEAHGEILYSAFFLEWFSEEARRVYGDIIHTPAKDRRALVLKQPIGVAAVITPWNFPSAM
  ITRKVGAALAAGCTVVVKPAEDTPFSALALAEVNQGFLLDLDPLLLASQAGIPSGVYNVIPCSRKNAKEV
  GEAICTDPLVSKISFTGSTTTGKILLHHAANSVKRVSMELGGLAPFIVFDSANVDQAVAGAMASKFRNTG
  QTCVCSNQFLVQRGIHDAFVKAFAEAMKKNLRVGNGFEEGTTQGPLINEKAVEKVEKQVNDAVSKGATVV
  TGGKRHQLGKNFFEPTLLCNVTQDMLCTHEETFGPLAPVIKFDTEEEAIAIANAADVGLAGYFYSQDPAQ
  IWRVAEQLEVGMVGVNEGLISSVECPFGGVKQSGLGREGSKYGIDEYLELKYVCYGGL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_170740
• ORF Size: 1644 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_170740.1, NP_733936.1
• RefSeq Size: 5170
• RefSeq ORF: 1647
• Locus ID: 7915
• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Butanoate metabolism, Metabolic pathways
• Gene Summary: This protein belongs to the aldehyde dehydrogenase family of proteins. This gene encodes a mitochondrial NAD(+)-dependent succinic semialdehyde dehydrogenase. A deficiency of this enzyme, known as 4-hydroxybutyricaciduria, is a rare inborn error in the metabolism of the neurotransmitter 4-aminobutyric acid (GABA). In response to the defect, physiologic fluids from patients accumulate GHB, a compound with numerous neuromodulatory properties. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]