AMPD1 (NM_000036) Human Tagged ORF Clone

CAT#: RG217919

AMPD1 (GFP-tagged) - Human adenosine monophosphate deaminase 1 (AMPD1), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: AMPD1
• Synonyms: MAD; MADA; MMDD
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217919 representing NM_000036
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTCTGTTCAAACTCCCAGCTGAAGAGAAACAAATTGATGATGCAATGCGCAACTTTGCTGAAAAAG
  TGTTTGCCTCTGAAGTCAAAGATGAAGGAGGTCGTCAGGAGATTTCCCCCTTTGATGTGGATGAGATCTG
  TCCGATTTCTCATCATGAGATGCAAGCACACATATTCCATCTGGAGACTCTGTCCACCTCCACAGAAGCC
  AGGAGAAAAAAGCGTTTCCAAGGACGGAAGACTGTTAATTTGTCCATTCCACTAAGTGAAACATCTTCCA
  CCAAACTGTCCCACATTGATGAATACATTTCCTCATCTCCAACCTACCAGACCGTGCCTGATTTTCAGAG
  AGTGCAGATTACTGGTGACTATGCCTCTGGGGTTACAGTTGAAGATTTTGAAATTGTTTGCAAAGGTCTG
  TATCGGGCACTATGCATACGTGAGAAATACATGCAGAAGTCGTTTCAGAGGTTCCCTAAAACCCCTTCCA
  AATACTTGCGGAACATTGATGGTGAGGCTTGGGTAGCAAATGAGAGCTTCTATCCAGTCTTTACTCCTCC
  TGTGAAGAAGGGAGAGGACCCCTTCCGAACAGACAACCTTCCTGAAAACCTGGGCTATCACCTCAAAATG
  AAGGACGGTGTAGTTTACGTCTATCCTAATGAAGCAGCAGTCAGCAAAGATGAGCCTAAGCCACTTCCTT
  ACCCAAATCTGGACACCTTCTTAGACGATATGAATTTTTTACTTGCTTTAATTGCTCAAGGACCTGTTAA
  GACCTATACCCACCGGCGCCTGAAGTTCCTCTCCTCCAAGTTCCAGGTCCATCAGATGCTTAACGAGATG
  GACGAGTTAAAGGAGCTGATAAACAACCCCCACCGAGATTTTTATAACTGCAGGAAGGTGGACACCCATA
  TCCATGCAGCCGCTTGCATGAACCAGAAACATCTGCTGCGTTTTATTAAGAAATCTTACCAAATTGATGC
  TGACAGAGTGGTCTATAGCACCAAAGAGAAGAATCTGACCCTAAAGGAACTTTTTGCTAAATTAAAAATG
  CATCCTTATGACCTGACTGTTGATTCTCTGGATGTTCATGCTGGACGCCAGACCTTCCAGCGTTTTGATA
  AGTTCAATGACAAATATAATCCTGTAGGAGCAAGTGAGCTACGGGACCTCTACTTGAAGACAGACAATTA
  CATTAATGGGGAATATTTTGCCACTATCATCAAGGAGGTAGGTGCGGACCTGGTGGAGGCCAAGTACCAG
  CATGCTGAGCCCCGCCTGTCCATCTATGGCCGCAGTCCTGATGAGTGGAGCAAACTCTCCTCCTGGTTCG
  TCTGCAATCGCATCCACTGCCCCAACATGACATGGATGATCCAGGTTCCCAGGATCTATGATGTGTTCCG
  TTCCAAGAATTTCCTTCCACATTTTGGAAAAATGCTGGAGAATATTTTCATGCCAGTGTTTGAGGCCACC
  ATCAACCCCCAGGCTGACCCAGAACTCAGTGTCTTCCTCAAGCATATCACTGGCTTTGACAGTGTGGATG
  ATGAGTCCAAACACAGTGGCCACATGTTCTCCTCCAAGAGTCCCAAGCCCCAGGAGTGGACATTGGAAAA
  GAATCCATCTTACACTTACTATGCCTACTACATGTATGCAAACATCATGGTGCTCAACAGCCTGAGAAAG
  GAACGAGGCATGAATACGTTTCTGTTCCGACCTCACTGTGGAGAAGCTGGAGCCCTCACCCATCTCATGA
  CAGCATTCATGATAGCAGATGATATCTCTCATGGCCTAAATTTAAAAAAGAGTCCCGTGCTACAGTACTT
  GTTTTTCTTAGCCCAAATTCCCATCGCCATGTCACCACTAAGTAACAATAGCCTATTTCTAGAGTATGCC
  AAAAATCCTTTTTTGGATTTCCTTCAGAAAGGGCTAATGATCTCACTGTCTACAGATGACCCAATGCAAT
  TCCACTTTACCAAGGAGCCCCTAATGGAAGAATATGCTATTGCTGCACAAGTCTTCAAGCTGAGCACCTG
  TGATATGTGCGAAGTGGCAAGGAACAGTGTCTTGCAGTGTGGAATTTCTCATGAGGAGAAAGTAAAGTTT
  CTGGGCGACAATTACCTTGAGGAAGGCCCTGCTGGAAATGATATCCGGAGGACAAATGTAGCCCAAATCC
  GCATGGCCTATCGCTATGAAACCTGGTGTTATGAACTCAATTTAATTGCTGAGGGTCTTAAATCAACAGA
  A
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000036
• ORF Size: 2241 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000036.1, NP_000027.1
• RefSeq Size: 2341 bp
• RefSeq ORF: 2244 bp
• Locus ID: 270
• Cytogenetics: 1p13.2
• Domains: A_deaminase
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Purine metabolism
• Gene Summary: 'Adenosine monophosphate deaminase 1 catalyzes the deamination of AMP to IMP in skeletal muscle and plays an important role in the purine nucleotide cycle. Two other genes have been identified, AMPD2 and AMPD3, for the liver- and erythocyte-specific isoforms, respectively. Deficiency of the muscle-specific enzyme is apparently a common cause of exercise-induced myopathy and probably the most common cause of metabolic myopathy in the human. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Feb 2010]'