AMPD1 (NM_000036) Human Untagged Clone

CAT#: SC113553

AMPD1 (untagged)-Human adenosine monophosphate deaminase 1 (AMPD1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AMPD1
• Synonyms: MAD; MADA; MMDD
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000036 edited
  GAATTCGGCACGAGGCAGTCACCCCACAGTCTCCTCTCTCTTCTTTTCTACTGTGCTATC
  CTAGAATCAAGGATTTCAGCAACAATGCCTCTGTTCAAACTCCCAGCTGAAGAGAAACAA
  ATTGATGATGCAATGCGCAACTTTGCTGAAAAAGTGTTTGCCTCTGAAGTCAAAGATGAA
  GGAGGTCGTCAGGAGATTTCCCCCTTTGATGTGGATGAGATCTGTCCGATTTCTCATCAT
  GAGATGCAAGCACACATATTCCATCTGGAGACTCTGTCCACCTCCACAGAAGCCAGGAGA
  AAAAAGCGTTTCCAAGGACGGAAGACTGTTAATTTGTCCATTCCACTAAGTGAAACATCT
  TCCACCAAACTGTCCCACATTGATGAATACATTTCCTCATCTCCAACCTACCAGACCGTG
  CCTGATTTTCAGAGAGTGCAGATTACTGGTGACTATGCCTCTGGGGTTACAGTTGAAGAT
  TTTGAAATTGTTTGCAAAGGTCTGTATCGGGCACTATGCATACGTGAGAAATACATGCAG
  AAGTCGTTTCAGAGGTTCCCTAAAACCCCTTCCAAATACTTGCGGAACATTGATGGTGAG
  GCTTGGGTAGCAAATGAGAGCTTCTATCCAGTCTTTACTCCTCCTGTGAAGAAGGGAGAG
  GACCCCTTCCGAACAGACAACCTTCCTGAAAACCTGGGCTATCACCTCAAAATGAAGGAC
  GGTGTAGTTTACGTCTATCCTAATGAAGCAGCAGTCAGCAAAGATGAGCCTAAGCCACTT
  CCTTACCCAAATCTGGACACCTTCTTAGACGATATGAATTTTTTACTTGCTTTAATTGCT
  CAAGGACCTGTTAAGACCTATACCCACCGGCGCCTGAAGTTCCTCTCCTCCAAGTTCCAG
  GTCCATCAGATGCTTAACGAGATGGACGAGTTAAAGGAGCTGATAAACAACCCCCACCGA
  GATTTTTATAACTGCAGGAAGGTGGACACCCATATCCATGCAGCCGCTTGCATGAACCAG
  AAACATCTGCTGCGTTTTATTAAGAAATCTTACCAAATTGATGCTGACAGAGTGGTCTAT
  AGCACCAAAGAGAAGAATCTGACCCTAAAGGAACTTTTTGCTAAATTAAAAATGCATCCT
  TATGACCTGACTGTTGATTCTCTGGATGTTCATGCTGGACGCCAGACCTTCCAGCGTTTT
  GATAAGTTCAATGACAAATATAATCCTGTAGGAGCAAGTGAGCTACGGGACCTCTACTTG
  AAGACAGACAATTACATTAATGGGGAATATTTTGCCACTATCATCAAGGAGGTAGGTGCG
  GACCTGGTGGAGGCCAAGTACCAGCATGCTGAGCCCCGCCTGTCCATCTATGGCCGCAGT
  CCTGATGAGTGGAGCAAACTCTCCTCCTGGTTCGTCTGCAATCGCATCCACTGCCCCAAC
  ATGACATGGATGATCCAGGTTCCCAGGATCTATGATGTGTTCCGTTCCAAGAATTTCCTT
  CCACATTTTGGAAAAATGCTGGAGAATATTTTCATGCCAGTGTTTGAGGCCACCATCAAC
  CCCCAGGCTGACCCAGAACTCAGTGTCTTCCTCAAGCATATCACTGGCTTTGACAGTGTG
  GATGATGAGTCCAAACACAGTGGCCACATGTTCTCCTCCAAGAGTCCCAAGCCCCAGGAG
  TGGACATTGGAAAAGAATCCATCTTACACTTACTATGCCTACTACATGTATGCAAACATC
  ATGGTGCTCAACAGCCTGAGAAAGGAACGAGGCATGAATACGTTTCTGTTCCGACCTCAC
  TGTGGAGAAGCTGGAGCCCTCACCCATCTCATGACAGCATTCATGATAGCAGATGATATC
  TCTCATGGCCTAAATTTAAAAAAGAGTCCCGTGCTACAGTACTTGTTTTTCTTAGCCCAA
  ATTCCCATCGCCATGTCACCACTAAGTAACAATAGCCTATTTCTAGAGTATGCCAAAAAT
  CCTTTTTTGGATTTCCTTCAGAAAGGGCTAATGATCTCACTGTCTACAGATGACCCAATG
  CAATTCCACTTTACCAAGGAGCCCCTAATGGAAGAATATGCTATTGCTGCACAAGTCTTC
  AAGCTGAGCACCTGTGATATGTGCGAAGTGGCAAGGAACAGTGTCTTGCAGTGTGGAATT
  TCTCATGAGGAGAAAGTAAAGTTTCTGGGCGACAATTACCTTGAGGAAGGCCCTGCTGGA
  AATGATATCCGGAGGACAAATGTAGCCCAAATCCGCATGGCCTATCGCTATGAAACCTGG
  TGTTATGAACTCAATTTAATTGCTGAGGGTCTTAAATCAACAGAATAAAAAAAAGTAAAC
  C
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000036 unedited
  TACTATAGGGCGGCCGCGAATTCGGCACGAGGCAGTCACCCCACAGTCTCCTCTCTCTTC
  TTTTCTACTGTGCTATCCTAGAATCAAGGATTTCAGCAACAATGCCTCTGTTCAAACTCC
  CAGCTGAAGAGAAACAAATTGATGATGCAATGCGCAACTTTGCTGAAAAAGTGTTTGCCT
  CTGAAGTCAAAGATGAAGGAGGTCGTCAGGAGATTTCCCCCTTTGATGTGGATGAGATCT
  GTCCGATTTCTCATCATGAGATGCAAGCACACATATTCCATCTGGAGACTCTGTCCACCT
  CCACAGAAGCCAGGAGAAAAAAGCGTTTCCAAGGACGGAAGACTGTTAATTTGTCCATTC
  CACTAAGTGAAACATCTTCCACCAAACTGTCCCACATTGATGAATACATTTCCTCATCTC
  CAACCTACCAGACCGTGCCTGATTNTCAGAGAGTGCAGATTACTGGTGACTATGCCTCTG
  GGGTTACAGTTGAAGATTNTGAAATTGTTTGCAAAGGTCTGTATCGGGCACTATGCATAC
  GTGAGAAATACATGCAGAAGTCGTTTCAGAGGTTCCCTANNACCCCTTCCAAATACTTGC
  NGAACATTGATGGTGAGGCTTGNGTAGCANATGAGAGCTTCTATCCAGTCTTTACTCCTN
  CTGTGAAGAAGGNNNAGAGACCCCTTNNCCGACAGACACCTTCCTGAANACCTGNGCTAT
  CACCTCAAAATGAANGGACGTGTAGNNTACGTCTATTCTAATGAAGCAGCAGTCAGCAAA
  GATGAGCCTAAGCCACTTTCTTTACCCAATCTGAACCCCTTCTTAGACGATATGAAATTT
  TTACTTGCTTTAANTGCTCAGGCACCTGTTAAGAACTATACCCNACCGNNCGCCTGAGTT
  CCTCTTCTTCAAGTTCCAGTCCATCAGATGCTTAACGAGATGGACGAGTTAAAGGAGCTG
  ATAAACACCCCNACCGAGATTTTNATACTGCAGAAGGTGGNACCCATTCCATGCAGCCGC
  TGCTGACCAGAACTNTGCTGCGTTTATTAGAATCTACAAATGTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000036 unedited
  TGGACCGCGCGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGTTTACTTTTTT
  TTATTCTGTTGATTTAAGACCCTCAGCAATTAAATTGAGTTCATAACACCAGGTTTCATA
  GCGATAGGCCATGCGGATTTGGGCTACATTTGTCCTCCGGATATCATTTCCAGCAGGGCC
  TTCCTCAAGGTAATTGTCGCCCAGAAACTTTACTTTCTCCTCATGAGAAATTCCACACTG
  CAAGACACTGTTCCTTGCCACTTCGCACATATCACAGGTGCTCAGCTTGAAGACTTGTGC
  AGCAATAGCATATTCTTCCATTAGGGGCTCCTTGGTAAAGTGGAATTGCATTGGGTCATC
  TGTAGACAGTGAGATCATTAGCCCTTTCTGAAGGAAATCCAAAAAAGGATTTTTGGCATA
  CTCTAGAAATAGGCTATTGTTACTTAGTGGCGACATGGCGATGGGAATTCGGGCTAAGAA
  AAACAAGTACTGTAGCTACGGGACTCTTTTTTAAATTTAGGCCATGAGAGATATCATCTG
  CTATCATGAATGCTGTCATGAGACGGGTGAGGGCTCCAGCTTCTCCACAGTGAGGTCGGA
  ACAGAAACGTATTCATGCCTTGTTCCTTTCTCAGGCTGTTGAGCACCATGATGTTTGCAT
  ACATGTAGTAGGCATACTAAGTGTCAGATGGCACCTTTTCCAATGTCCACTCCTGGGGCT
  TGGGACTCTTGGAGGAGAACATGCGGCCACTGTGTTTCGACTCATCATTCACCCTGCCCA
  ACCCAGTGACATGCTTTGACGAAGACACTGAGTTCTGGGGCAANCTGGCGGCTGATGGTG
  GCCTCAAACACTGCCATGAAATTATTTTCAGCATTTTCCCAAATTGGAAAGAAATTTTGC
  CACGGACCATCATTAAACCGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000036
• Insert Size: 2430 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000036.1, NP_000027.1
• RefSeq Size: 2341 bp
• RefSeq ORF: 2244 bp
• Locus ID: 270
• Cytogenetics: 1p13.2
• Domains: A_deaminase
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Purine metabolism
• Gene Summary: 'Adenosine monophosphate deaminase 1 catalyzes the deamination of AMP to IMP in skeletal muscle and plays an important role in the purine nucleotide cycle. Two other genes have been identified, AMPD2 and AMPD3, for the liver- and erythocyte-specific isoforms, respectively. Deficiency of the muscle-specific enzyme is apparently a common cause of exercise-induced myopathy and probably the most common cause of metabolic myopathy in the human. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Feb 2010]'
  Transcript Variant: This variant (1) encodes the longer isoform (1).