Plakophilin 2 (PKP2) (NM_004572) Human Tagged ORF Clone

CAT#: RG218592

PKP2 (GFP-tagged) - Human plakophilin 2 (PKP2), transcript variant 2b

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PKP2
• Synonyms: ARVD9
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG218592 representing NM_004572
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCCCCCGGCGCCCCAGCTGAGTACGGCTACATCCGGACCGTCCTGGGCCAGCAGATCCTGGGAC
  AACTGGACAGCTCCAGCCTGGCGCTGCCCTCCGAGGCCAAGCTGAAGCTGGCGGGGAGCAGCGGCCGCGG
  CGGCCAGACAGTCAAGAGCCTGCGGATCCAGGAGCAGGTGCAGCAGACCCTCGCCCGGAAGGGCCGCAGC
  TCCGTGGGCAACGGAAATCTTCACCGAACCAGCAGTGTTCCTGAGTATGTCTACAACCTACACTTGGTTG
  AAAATGATTTTGTTGGAGGCCGTTCCCCTGTTCCTAAAACCTATGACATGCTAAAGGCTGGCACAACTGC
  CACTTATGAAGGTCGCTGGGGAAGAGGAACAGCACAGTACAGCTCCCAGAAGTCCGTGGAAGAAAGGTCC
  TTGAGGCATCCTCTGAGGAGACTGGAGATTTCTCCTGACAGCAGCCCGGAGAGGGCTCACTACACGCACA
  GCGATTACCAGTACAGCCAGAGAAGCCAGGCTGGGCACACCCTGCACCACCAAGAAAGCAGGCGGGCCGC
  CCTCCTAGTGCCACCGAGATATGCTCGTTCCGAGATCGTGGGGGTCAGCCGTGCTGGCACCACAAGCAGG
  CAGCGCCACTTTGACACATACCACAGACAGTACCAGCATGGCTCTGTTAGCGACACCGTTTTTGACAGCA
  TCCCTGCCAACCCGGCCCTGCTCACGTACCCCAGGCCAGGGACCAGCCGCAGCATGGGCAACCTCTTGGA
  GAAGGAGAACTACCTGACGGCAGGGCTCACTGTCGGGCAGGTCAGGCCGCTGGTGCCCCTGCAGCCCGTC
  ACTCAGAACAGGGCTTCCAGGTCCTCCTGGCATCAGAGCTCCTTCCACAGCACCCGCACGCTGAGGGAAG
  CTGGGCCCAGTGTCGCCGTGGATTCCAGCGGGAGGAGAGCGCACTTGACTGTCGGCCAGGCGGCCGCAGG
  GGGAAGTGGGAATCTGCTCACTGAGAGAAGCACTTTCACTGACTCCCAGCTGGGGAATGCAGACATGGAG
  ATGACTCTGGAGCGAGCAGTGAGTATGCTCGAGGCAGACCACATGCCGCCATCCAGGATTTCTGCTGCAG
  CTACTTTCATACAGCACGAGTGCTTCCAGAAATCTGAAGCTCGGAAGAGGGTTAACCAGCTTCGTGGCAT
  CCTCAAGCTTCTGCAGCTCCTAAAAGTTCAGAATGAAGACGTTCAGCGAGCTGTGTGTGGGGCCTTGAGA
  AACTTAGTATTTGAAGACAATGACAACAAATTGGAGGTGGCTGAACTAAATGGGGTACCTCGGCTGCTCC
  AGGTGCTGAAGCAAACCAGAGACTTGGAGACTAAAAAACAAATAACAGACCATACAGTCAATTTAAGAAG
  TAGGAATGGCTGGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGCGGGCGG
  ATCACGAGGTCAGGAGTTCGAGACCAGCCTGACCAACATGGTTTGCTGTGGAATTTGTCATCTAATGACA
  AACTCAAGAATCTCATGATAACAGAAGCATTGCTTACGCTGACGGAGAATATCATCATCCCCTTTTCTGG
  GTGGCCTGAAGGAGACTACCCAAAAGCAAATGGTTTGCTCGATTTTGACATATTCTACAACGTCACTGGA
  TGCCTAAGAAACATGAGTTCTGCTGGCGCTGATGGGAGAAAAGCGATGAGAAGATGTGACGGACTCATTG
  ACTCACTGGTCCATTATGTCAGAGGAACCATTGCAGATTACCAGCCAGATGACAAGGCCACGGAGAATTG
  TGTGTGCATTCTTCATAACCTCTCCTACCAGCTGGAGGCAGAGCTCCCAGAGAAATATTCCCAGAATATC
  TATATTCAAAACCGGAATATCCAGACTGACAACAACAAAAGTATTGGATGTTTTGGCAGTCGAAGCAGGA
  AAGTAAAAGAGCAATACCAGGACGTGCCGATGCCGGAGGAAAAGAGCAACCCCAAGGGCGTGGAGTGGCT
  GTGGCATTCCATTGTTATAAGGATGTATCTGTCCTTGATCGCCAAAAGTGTCCGCAACTACACACAAGAA
  GCATCCTTAGGAGCTCTGCAGAACCTCACGGCCGGAAGTGGACCAATGCCGACATCAGTGGCTCAGACAG
  TTGTCCAGAAGGAAAGTGGCCTGCAGCACACCCGAAAGATGCTGCATGTTGGTGACCCAAGTGTGAAAAA
  GACAGCCATCTCGCTGCTGAGGAATCTGTCCCGGAATCTTTCTCTGCAGAATGAAATTGCCAAAGAAACT
  CTCCCTGATTTGGTTTCCATCATTCCTGACACAGTCCCGAGTACTGACCTTCTCATTGAAACTACAGCCT
  CTGCCTGTTACACATTGAACAACATAATCCAAAACAGTTACCAGAATGCACGCGACCTTCTAAACACCGG
  GGGCATCCAGAAAATTATGGCCATTAGTGCAGGCGATGCCTATGCCTCCAACAAAGCAAGTAAAGCTGCT
  TCCGTCCTTCTGTATTCTCTGTGGGCACACACGGAACTGCATCATGCCTACAAGAAGGCTCAGTTTAAGA
  AGACAGATTTTGTCAACAGCCGGACTGCCAAAGCCTACCACTCCCTTAAAGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG218592 representing NM_004572
  Red=Cloning site Green=Tags(s)
  
  MAAPGAPAEYGYIRTVLGQQILGQLDSSSLALPSEAKLKLAGSSGRGGQTVKSLRIQEQVQQTLARKGRS
  SVGNGNLHRTSSVPEYVYNLHLVENDFVGGRSPVPKTYDMLKAGTTATYEGRWGRGTAQYSSQKSVEERS
  LRHPLRRLEISPDSSPERAHYTHSDYQYSQRSQAGHTLHHQESRRAALLVPPRYARSEIVGVSRAGTTSR
  QRHFDTYHRQYQHGSVSDTVFDSIPANPALLTYPRPGTSRSMGNLLEKENYLTAGLTVGQVRPLVPLQPV
  TQNRASRSSWHQSSFHSTRTLREAGPSVAVDSSGRRAHLTVGQAAAGGSGNLLTERSTFTDSQLGNADME
  MTLERAVSMLEADHMPPSRISAAATFIQHECFQKSEARKRVNQLRGILKLLQLLKVQNEDVQRAVCGALR
  NLVFEDNDNKLEVAELNGVPRLLQVLKQTRDLETKKQITDHTVNLRSRNGWPGAVAHACNPSTLGGQGGR
  ITRSGVRDQPDQHGLLWNLSSNDKLKNLMITEALLTLTENIIIPFSGWPEGDYPKANGLLDFDIFYNVTG
  CLRNMSSAGADGRKAMRRCDGLIDSLVHYVRGTIADYQPDDKATENCVCILHNLSYQLEAELPEKYSQNI
  YIQNRNIQTDNNKSIGCFGSRSRKVKEQYQDVPMPEEKSNPKGVEWLWHSIVIRMYLSLIAKSVRNYTQE
  ASLGALQNLTAGSGPMPTSVAQTVVQKESGLQHTRKMLHVGDPSVKKTAISLLRNLSRNLSLQNEIAKET
  LPDLVSIIPDTVPSTDLLIETTASACYTLNNIIQNSYQNARDLLNTGGIQKIMAISAGDAYASNKASKAA
  SVLLYSLWAHTELHHAYKKAQFKKTDFVNSRTAKAYHSLKD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004572
• ORF Size: 2643 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004572.2, NP_004563.1
• RefSeq Size: 4431 bp
• RefSeq ORF: 2646 bp
• Locus ID: 5318
• Cytogenetics: 12p11.21
• Domains: Armadillo_seg
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC)
• Gene Summary: 'This gene encodes a member of the arm-repeat (armadillo) and plakophilin gene families. Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the cytoskeleton. This gene product may regulate the signaling activity of beta-catenin. Two alternately spliced transcripts encoding two protein isoforms have been identified. A processed pseudogene with high similarity to this locus has been mapped to chromosome 12p13. [provided by RefSeq, Jul 2008]'