Plakophilin 2 (PKP2) (NM_004572) Human Untagged Clone

CAT#: SC117285

PKP2 (untagged)-Human plakophilin 2 (PKP2), transcript variant 2b


  "NM_004572" in other vectors (4)

Reconstitution Protocol

USD 1,790.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PKP2
Synonyms ARVD9
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_004572, the custom clone sequence may differ by one or more nucleotides


ATGGCAGCCCCCGGCGCCCCAGCTGAGTACGGCTACATCCGGACCGTCCTGGGCCAGCAGATCCTGGGAC
AACTGGACAGCTCCAGCCTGGCGCTGCCCTCCGAGGCCAAGCTGAAGCTGGCGGGGAGCAGCGGCCGCGG
CGGCCAGACAGTCAAGAGCCTGCGGATCCAGGAGCAGGTGCAGCAGACCCTCGCCCGGAAGGGCCGCAGC
TCCGTGGGCAACGGAAATCTTCACCGAACCAGCAGTGTTCCTGAGTATGTCTACAACCTACACTTGGTTG
AAAATGATTTTGTTGGAGGCCGTTCCCCTGTTCCTAAAACCTATGACATGCTAAAGGCTGGCACAACTGC
CACTTATGAAGGTCGCTGGGGAAGAGGAACAGCACAGTACAGCTCCCAGAAGTCCGTGGAAGAAAGGTCC
TTGAGGCATCCTCTGAGGAGACTGGAGATTTCTCCTGACAGCAGCCCGGAGAGGGCTCACTACACGCACA
GCGATTACCAGTACAGCCAGAGAAGCCAGGCTGGGCACACCCTGCACCACCAAGAAAGCAGGCGGGCCGC
CCTCCTAGTGCCACCGAGATATGCTCGTTCCGAGATCGTGGGGGTCAGCCGTGCTGGCACCACAAGCAGG
CAGCGCCACTTTGACACATACCACAGACAGTACCAGCATGGCTCTGTTAGCGACACCGTTTTTGACAGCA
TCCCTGCCAACCCGGCCCTGCTCACGTACCCCAGGCCAGGGACCAGCCGCAGCATGGGCAACCTCTTGGA
GAAGGAGAACTACCTGACGGCAGGGCTCACTGTCGGGCAGGTCAGGCCGCTGGTGCCCCTGCAGCCCGTC
ACTCAGAACAGGGCTTCCAGGTCCTCCTGGCATCAGAGCTCCTTCCACAGCACCCGCACGCTGAGGGAAG
CTGGGCCCAGTGTCGCCGTGGATTCCAGCGGGAGGAGAGCGCACTTGACTGTCGGCCAGGCGGCCGCAGG
GGGAAGTGGGAATCTGCTCACTGAGAGAAGCACTTTCACTGACTCCCAGCTGGGGAATGCAGACATGGAG
ATGACTCTGGAGCGAGCAGTGAGTATGCTCGAGGCAGACCACATGCTGCCATCCAGGATTTCTGCTGCAG
CTACTTTCATACAGCACGAGTGCTTCCAGAAATCTGAAGCTCGGAAGAGGGTTAACCAGCTTCGTGGCAT
CCTCAAGCTTCTGCAGCTCCTAAAAGTTCAGAATGAAGACGTTCAGCGAGCTGTGTGTGGGGCCTTGAGA
AACTTAGTATTTGAAGACAATGACAACAAATTGGAGGTGGCTGAACTAAATGGGGTACCTCGGCTGCTCC
AGGTGCTGAAGCAAACCAGAGACTTGGAGACTAAAAAACAAATAACAGACCATACAGTCAATTTAAGAAG
TAGGAATGGCTGGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGCGGGCGG
ATCACGAGGTCAGGAGTTCGAGACCAGCCTGACCAACATGGTTTGCTGTGGAATTTGTCATCTAATGACA
AACTCAAGAATCTCATGATAACAGAAGCATTGCTTACGCTGACGGAGAATATCATCATCCCCTTTTCTGG
GTGGCCTGAAGGAGACTACCCAAAAGCAAATGGTTTGCTCGATTTTGACATATTCTACAACGTCACTGGA
TGCCTAAGAAACATGAGTTCTGCTGGCGCTGATGGGAGAAAAGCGATGAGAAGATGTGACGGACTCATTG
ACTCACTGGTCCATTATGTCAGAGGAACCATTGCAGATTACCAGCCAGATGACAAGGCCACGGAGAATTG
TGTGTGCATTCTTCATAACCTCTCCTACCAGCTGGAGGCAGAGCTCCCAGAGAAATATTCCCAGAATATC
TATATTCAAAACCGGAATATCCAGACTGACAACAACAAAAGTATTGGATGTTTTGGCAGTCGAAGCAGGA
AAGTAAAAGAGCAATACCAGGACGTGCCGATGCCGGAGGAAAAGAGCAACCCCAAGGGCGTGGAGTGGCT
GTGGCATTCCATTGTTATAAGGATGTATCTGTCCTTGATCGCCAAAAGTGTCCGCAACTACACACAAGAA
GCATCCTTAGGAGCTCTGCAGAACCTCACGGCCGGAAGTGGACCAATGCCGACATCAGTGGCTCAGACAG
TTGTCCAGAAGGAAAGTGGCCTGCAGCACACCCGAAAGATGCTGCATGTTGGTGACCCAAGTGTGAAAAA
GACAGCCATCTCGCTGCTGAGGAATCTGTCCCGGAATCTTTCTCTGCAGAATGAAATTGCCAAAGAAACT
CTCCCTGATTTGGTTTCCATCATTCCTGACACAGTCCCGAGTACTGACCTTCTCATTGAAACTACAGCCT
CTGCCTGTTACACATTGAACAACATAATCCAAAACAGTTACCAGAATGCACGCGACCTTCTAAACACCGG
GGGCATCCAGAAAATTATGGCCATTAGTGCAGGCGATGCCTATGCCTCCAACAAAGCAAGTAAAGCTGCT
TCCGTCCTTCTGTATTCTCTGTGGGCACACACGGAACTGCATCATGCCTACAAGAAGGCTCAGTTTAAGA
AGACAGATTTTGTCAACAGCCGGACTGCCAAAGCCTACCACTCCCTTAAAGACTGA


>OriGene 5' read for NM_004572 unedited
NTTTTTCGNAATTATGTATACGAACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCAG
CTCGGTCGCCCCCACCGGCCCCATGGCAGCCCCCGGCGCCCCAGCTGAGTACGGCTACAT
CCGGACCGTCCTGGGCCAGCAGATCCTGGGACAACTGGACAGCTCCAGCCTGGCGCTGCC
CTCCGAGGCCAAGCTGAAGCTGGCGGGGAGCAGCGGCCGCGGCGGCCAGACAGTCAAGAG
CCTGCGGATCCAGGAGCAGGTGCAGCAGACCCTCGCCCGGAAGGGCCGCAGCTCCGTGGG
CAACGGAAATCTTCACCGAACCAGCAGTGTTCCTGAGTATGTCTACAACCTACACTTGGT
TGAAAATGATTTTGTTGGAGGCCGTTCCCCTGTTCCTAAAACCTATGACATGCTAAAGGC
TGGCACAACTGCCACTTATGAAGGTCGCTGGGGAAGAGGAACAGCACAGTACAGCTCCCA
GAAGTCCGTGGAAGAAAGGTCCTTGAGGCATCCTCTGAGGAGACTGGAGATTTCTCCTGA
CAGCAGCCCGGAGAGGGCTCACTACACGCACAGCGATTACCAGTACAGCCAGAGAAGCCA
GGCTGGGCACACCCTGCACCACCAAGAAAGCAGGCGGGCCGCCCTCCTAGTGCCACCGAG
ATATGCTCGTTCCGAGATCGTGGNGGTCAGCCGTGCTGGCACCACAAGCAGGCAGCGCCA
CTNTGACACATACCACAGACAGTACCAGCATGGCTCTGTTAGCGACACCGTTTTTGACAG
CATCCCTGCCAACCCGGCCCTGCTCACGTACCCCCAGCCAGGGACCAGCCGCAGCATGGG
CAACCTCTGGNAGAAGGAGAACTACCTCGACGCAGGNCTCACTGTCGGCCCAGTCAGCCC
GCTGGTGCCCCTGCAGCCGTCACTCAGAACAGGCTTTCCAGTCCTCCTGGCATAAC
>OriGene 3' read for NM_004572 unedited
TACCTAGCTTCNNGTCNAGGTCGAGTTTATTACTTTTTTTTTTAAATTATCGAATGCTTT
ATTAATGCCTTAAAAAACACAAAAGTTCTGAACACACATTTATTATCTGGAGGAATAAAT
GCACTCCAAATGCTTATCATCAGAACTCTGGAAAAAGAATATAAATTCTCTCCAGTATTT
TAAAAATTGGTATCTAAATAATTGCGGTGGGAACTAGGTTAGCAACCTAAGTTGGCTTCA
TTCAGCTTTTAAAATTGAAAAAAAATTGCTTCAAATATAATAAAGACTTAAGTACACATT
CAAAATTTATTTTCTGTTACAGTTTTCTTGCCAAAAGGAGTTGAAGAATTTTTTTGCTTA
TCTTACTTAGGACAAATGTTGTCAGATTAAAAAAAAAAATAACAGAATAGTAGTTGTAAA
GACTAACTCTATATGAATGGTTCTAATCATAGCGCAGTTAAAACAACATTTAATGTACAT
TCATTTCGCTGGATGATGTCACCCATTTAAAATATCACGATTTCCCATATCATCGCTGGA
TGGTCTTGACACATTCTTACATCTTTTTCAACATGACTTCTTCCACTTTCCTTCTCTAGA
GTTTTCTTGAGTTAAAATACTGGTCTTGCAGAAAACATTTGCCATCCAGCGGCCCCGCGA
TATTTGTTAACCGGCGGGGCAAGGCTGCGCTGGCTGCCCATGCCCCTCATTATCCTGAAC
CCGCTTTCCCCCGCCCCACGCTGGCCCCCCTCCCTGTTCCCGGGTCTCCCTCCTTTTCCC
CCTCCCATTCGATTCTTCTTTTTCCTATTCCCGGCCTCGCCCCCCGACCCCCCACCCGCC
TCCGTCTTATCCCCCGCCCGCGACCATTATGCCCCCACGTTTCTTTCCCGCCTCCCCTTC
CCCCAATTTGTCTTTTTTTTTCTGCCCCCCCCCCGTCCCTTTTCCGCCCCTTTTTGTTCA
TTCCCCCCCTCAATCCACACCTAA
Restriction Sites NotI-NotI     
ACCN NM_004572
Insert Size 4230 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004572.2, NP_004563.1
RefSeq Size 4439 bp
RefSeq ORF 2646 bp
Locus ID 5318
Cytogenetics 12p11.21
Domains Armadillo_seg
Protein Pathways Arrhythmogenic right ventricular cardiomyopathy (ARVC)
Gene Summary 'This gene encodes a member of the arm-repeat (armadillo) and plakophilin gene families. Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the cytoskeleton. This gene product may regulate the signaling activity of beta-catenin. Two alternately spliced transcripts encoding two protein isoforms have been identified. A processed pseudogene with high similarity to this locus has been mapped to chromosome 12p13. [provided by RefSeq, Jul 2008]'
Transcript Variant: This transcript variant (2b) represents the longer transcript variant and encodes the longer isoform.

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