Apc7 (ANAPC7) (NM_016238) Human Tagged ORF Clone

CAT#: RG219871

ANAPC7 (GFP-tagged) - Human anaphase promoting complex subunit 7 (ANAPC7), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ANAPC7
• Synonyms: APC7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219871 representing NM_016238
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGTGATAGACCACGTGCGGGACATGGCGGCCGCGGGGCTGCACTCCAACGTGCGGCTCCTCAGCA
  GCTTGTTACTTACAATGAGTAATAACAACCCTGAGTTATTCTCCCCACCTCAGAAGTACCAGCTTTTGGT
  GTATCATGCAGATTCTCTCTTTCATGATAAGGAATATCGGAATGCTGTGAGTAAGTATACCATGGCTTTA
  CAGCAGAAGAAAGCGCTAAGTAAAACTTCAAAAGTGAGACCTTCAACTGGAAATTCTGCATCTACTCCAC
  AAAGTCAGTGTCTTCCATCTGAAATTGAAGTGAAATACAAAATGGCTGAATGTTATACAATGCTAAAACA
  AGATAAAGATGCCATTGCTATACTTGATGGGATCCCTTCAAGACAAAGAACTCCCAAAATAAACATGATG
  CTGGCAAACCTGTACAAGAAGGCTGGTCGGGAGCGCCCTTCAGTCACCAGCTATAAGGAGGTGCTGAGGC
  AGTGCCCATTAGCCCTTGATGCCATTCTAGGCTTGTTGTCCCTTTCTGTAAAAGGGGCAGAGGTGGCATC
  CATGACAATGAATGTGATCCAAACCGTGCCTAACTTGGACTGGCTCTCTGTGTGGATCAAAGCGTATGCT
  TTTGTGCACACTGGTGACAACTCAAGAGCAATCAGTACCATCTGTTCACTAGAGAAAAAATCCTTATTGC
  GAGATAACGTGGACCTATTGGGAAGCTTGGCAGATCTGTACTTCAGAGCTGGAGACAATAAAAACTCTGT
  CCTCAAGTTTGAACAGGCACAGATGTTGGATCTTTATCTGATAAAAGGAATGGATGTATATGGCTACCTA
  CTGGCACGAGAAGGGCGGCTAGAGGATGTTGAGAACCTTGGATGCCGCCTTTTCAATATCTCTGATCAGC
  ATGCAGAACCGTGGGTGGTTTCTGGCTGTCACAGCTTCTATAGCAAACGCTACTCCCGGGCCCTCTATTT
  AGGAGCCAAGGCCATTCAGCTGAACAGTAATAGTGTTCAAGCTCTGCTACTTAAGGGAGCAGCACTTAGG
  AACATGGGCAGAGTCCAAGAAGCAATAATCCACTTTCGGGAGGCCATACGGCTCGCACCTTGTCGCTTAG
  ATTGTTATGAAGGTCTTATCGAATGTTACTTAGCCTCCAACAGTATTCGAGAAGCAATGGTAATGGCTAA
  CAACGTTTACAAAACTCTGGGAGCAAATGCACAGACCCTTACCCTTTTAGCCACCGTTTGTCTTGAAGAC
  CCAGTGACACAGGAGAAAGCCAAAACATTATTAGATAAAGCCCTGACCCAAAGGCCAGATTACATTAAGG
  CTGTGGTGAAAAAAGCAGAACTACTTAGCAGAGAACAGAAATATGAAGATGGAATTGCTTTGCTGAGGAA
  CGCACTGGCTAATCAGAGTGACTGTGTCCTGCATCGGATCCTAGGAGATTTCCTTGTAGCTGTCAATGAG
  TATCAGGAGGCAATGGACCAGTATAGTATAGCACTAAGTTTGGACCCCAATGACCAGAAGTCTCTAGAGG
  GGATGCAGAAGATGGAGAAGGAGGAGAGTCCCACGGATGCCACTCAGGAGGAGGATGTGGACGACATGGA
  AGGGAGTGGGGAAGAAGGGGACCTGGAGGGCAGCGACAGTGAGGCGGCCCAGTGGGCTGACCAGGAGCAG
  TGGTTCGGCATGCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219871 representing NM_016238
  Red=Cloning site Green=Tags(s)
  
  MNVIDHVRDMAAAGLHSNVRLLSSLLLTMSNNNPELFSPPQKYQLLVYHADSLFHDKEYRNAVSKYTMAL
  QQKKALSKTSKVRPSTGNSASTPQSQCLPSEIEVKYKMAECYTMLKQDKDAIAILDGIPSRQRTPKINMM
  LANLYKKAGRERPSVTSYKEVLRQCPLALDAILGLLSLSVKGAEVASMTMNVIQTVPNLDWLSVWIKAYA
  FVHTGDNSRAISTICSLEKKSLLRDNVDLLGSLADLYFRAGDNKNSVLKFEQAQMLDLYLIKGMDVYGYL
  LAREGRLEDVENLGCRLFNISDQHAEPWVVSGCHSFYSKRYSRALYLGAKAIQLNSNSVQALLLKGAALR
  NMGRVQEAIIHFREAIRLAPCRLDCYEGLIECYLASNSIREAMVMANNVYKTLGANAQTLTLLATVCLED
  PVTQEKAKTLLDKALTQRPDYIKAVVKKAELLSREQKYEDGIALLRNALANQSDCVLHRILGDFLVAVNE
  YQEAMDQYSIALSLDPNDQKSLEGMQKMEKEESPTDATQEEDVDDMEGSGEEGDLEGSDSEAAQWADQEQ
  WFGMQ
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016238
• ORF Size: 1695 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_016238.1, NP_057322.1
• RefSeq Size: 1698
• RefSeq ORF: 1800
• Locus ID: 51434
• Domains: TPR
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis
• Gene Summary: This gene encodes a tetratricopeptide repeat containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for proper protein ubiquitination function of APC/C and for the interaction of APC/C with certain transcription coactivators. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008]