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Myosin (MYH4) (NM_017533) Human Tagged ORF Clone
CAT#: RG220101
- TrueORF®
MYH4 (GFP-tagged) - Human myosin, heavy chain 4, skeletal muscle (MYH4)
"NM_017533" in other vectors (2)
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Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Symbol | MYH4 |
| Synonyms | MYH2B; MyHC-2B; MyHC-IIb |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG220101 representing NM_017533
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTCTGACTCTGAGATGGCCATTTTTGGGGAGGCTGCTCCTTTCCTCCGAAAGTCTGAAAAGGAGC GAATTGAAGCTCAGAACAAGCCTTTTGATGCCAAGACATCAGTCTTTGTGGTGGACCCTAAGGAGTCCTA CGTGAAAGCAATAGTGCAGAGCAGGGAAGGGGGGAAGGTGACAGCCAAGACCGAAGCTGGAGCTACTGTA ACTGTGAAAGAAGACCAAGTCTTCTCCATGAACCCTCCCAAATATGACAAGATCGAGGACATGGCCATGA TGACTCACCTGCATGAGCCTGCTGTGCTGTATAACCTCAAAGAGCGTTACGCAGCCTGGATGATCTACAC CTACTCGGGCCTCTTCTGTGTCACCGTCAACCCCTACAAGTGGCTGCCGGTGTACAACCCTGAGGTGGTG ACAGCCTACCGAGGCAAAAAGCGCCAGGAGGCCCCACCCCATATCTTCTCCATCTCTGACAATGCCTATC AGTTCATGCTAACTGATCGTGAAAACCAGTCAATCTTGATTACTGGAGAATCTGGTGCAGGGAAGACTGT GAACACGAAGCGTGTCATCCAGTACTTTGCAACAATTGCAGTTACTGGAGAGAAGAAAAAAGAGGAACCT GCCTCTGGCAAAATGCAGGGGACCCTTGAAGATCAAATCATCAGTGCTAACCCCCTACTGGAAGCCTTCG GCAATGCCAAGACCGTGAGGAATGACAACTCCTCTCGCTTTGGTAAATTCATCAGGATCCATTTTGGTGC CACAGGCAAACTGGCTTCTGCAGATATTGAAACATATCTGCTAGAGAAGTCCCGAGTTACTTTTCAGCTA AAGGCTGAAAGAAGCTACCACATATTTTATCAAATCCTGTCCAATAAGAAACCAGAGCTCATTGAAATGC TTCTGATCACCACCAACCCATATGACTTCGCATTTGTCAGCCAAGGGGAAATTACTGTGCCCAGCATTGA TGACCAGGAAGAGCTGATGGCCACAGATAGTGCTGTGGACATCCTGGGTTTCACTGCTGATGAAAAGGTG GCCATTTACAAGCTCACTGGAGCCGTGATGCATTATGGGAACATGAAATTCAAGCAAAAGCAAAGGGAAG AGCAGGCAGAGCCAGATGGCACGGAAGTTGCTGACAAAGCTGCTTATCTGACAAGTCTGAACTCTGCTGA CCTGCTCAAATCTCTCTGCTATCCCAGAGTCAAGGTCGGCAATGAGTTCGTAACCAAAGGCCAGACTGTG CAGCAGGTGTACAACGCAGTGGGTGCTCTGGCCAAAGCCATCTACGAGAAGATGTTCCTGTGGATGGTCA CCCGCATCAACCAGCAGCTGGACACCAAGCAGCCCAGGCAGTACTTCATCGGGGTCTTGGACATTGCTGG CTTTGAGATCTTTGATTTCAACAGCCTGGAGCAGCTGTGCATCAACTTCACCAACGAGAAACTGCAACAG TTTTTCAACCACCACATGTTCGTGCTGGAGCAGGAAGAGTACAAGAAGGAAGGCATCGAGTGGGAGTTCA TTGACTTCGGGATGGACCTGGCTGCCTGCATCGAGCTCATCGAGAAGCCTATGGGCATCTTCTCCATCCT AGAAGAGGAGTGCATGTTCCCCAAGGCAACAGACACCTCCTTCAAGAACAAGCTGTATGAACAACATCTT GGAAAATCCAACAACTTCCAGAAGCCCAAGCCTGCCAAAGGCAAGCCTGAGGCTCACTTCTCACTGGTGC ACTATGCCGGCACCGTGGACTACAACATCGCCGGCTGGCTGGACAAAAACAAGGACCCCCTGAATGAGAC TGTGGTGGGGCTGTACCAGAAGTCTGCAATGAAGACTCTGGCTTTCCTCTTCTCTGGGGCACAAACTGCT GAAGCAGAGGGTGGTGGTGGAAAGAAAGGTGGCAAAAAGAAGGGTTCTTCTTTCCAGACAGTGTCAGCTC TTTTCAGGGAGAATTTGAATAAGCTGATGACCAACTTGAGGAGCACTCACCCCCACTTTGTGCGGTGCAT CATCCCCAATGAAACTAAAACTCCTGGTGCCATGGAGCATGAGCTTGTCCTGCATCAGCTGAGGTGTAAC GGTGTGCTGGAAGGCATCCGCATCTGCAGGAAAGGCTTCCCAAGCAGAATCCTTTATGCAGACTTCAAAC AGAGATACAAGGTTCTAAATGCGAGTGCTATCCCAGAGGGTCAGTTCATTGACAGCAAGAAGGCTTCTGA GAAACTTCTAGGGTCTATTGAAATTGACCACACCCAGTACAAATTCGGTCATACCAAGGTTTTCTTCAAA GCTGGCCTGCTGGGAACTCTAGAAGAAATGCGAGATGAAAAGCTAGCTCAACTCATCACGCGCACTCAAG CCATATGCAGGGGGTTCCTGATGAGAGTGGAGTTCAGAAAGATGATGGAGAGGAGAGAGTCCATCTTCTG CATTCAGTACAACATCCGTGCTTTCATGAATGTGAAGCACTGGCCCTGGATGAAGCTGTATTTCAAGATC AAGCCCCTCCTCAAGAGTGCAGAGACAGAGAAGGAGATGGCCAACATGAAGGAAGAATTTGAGAAAACCA AAGAAGAGCTGGCTAAGACAGAGGCAAAAAGGAAAGAACTAGAAGAAAAGATGGTGACGCTAATGCAAGA GAAAAATGACTTACAACTCCAAGTTCAAGCTGAAGCAGATGCCTTGGCTGATGCAGAGGAAAGATGTGAT CAGTTGATTAAAACCAAAATCCAACTTGAGGCCAAAATCAAAGAGGTAACTGAAAGAGCTGAGGATGAGG AAGAGATCAATGCTGAGCTGACAGCCAAGAAGAGGAAACTGGAGGATGAATGTTCAGAGCTCAAGAAAGA CATTGATGACCTTGAGCTGACACTGGCCAAGGTTGAGAAGGAGAAACATGCCACAGAGAACAAGGTGAAA AACCTCACAGAAGAGATGGCAGGTCTGGATGAAACCATTGCTAAGCTGACCAAGGAGAAGAAGGCTCTCC AGGAGGCCCACCAGCAGACCCTGGATGACCTGCAGATGGAGGAGGACAAAGTCAACACCCTGACCAAAGC TAAAACCAAGCTAGAACAGCAAGTGGACGATCTTGAAGGATCTCTGGAACAAGAAAAGAAACTTTGCATG GACTTAGAAAGAGCCAAGAGAAAACTGGAGGGTGACCTAAAATTGGCCCAAGAATCCACAATGGATACAG AAAATGACAAACAGCAACTTAATGAGAAACTCAAAAAGAAAGAGTTTGAAATGAGCAATCTGCAAGGCAA GATTGAAGATGAACAAGCCCTTGCAATGCAGCTACAAAAGAAGATCAAAGAATTACAGGCCCGCATTGAG GAGCTGGAGGAGGAAATCGAGGCAGAGCGGGCCTCCCGGGCCAAAGCAGAAAAGCAGCGCTCTGACCTCT CCCGGGAGCTGGAGGAGATCAGTGAGAGGCTGGAAGAAGCCGGTGGGGCCACTTCAGCCCAGATTGAGTT GAACAAGAAGCGGGAGGCTGAGTTCCAGAAAATGCGCAGGGACCTGGAAGAGTCCACCCTGCAGCACGAA GCCACGGCAGCTGCTCTTCGGAAGAAGCACGCAGATAGTGTGGCTGAGCTTGGGAAGCAGATCGACAGCC TTCAGCGGGTCAAGCAGAAGCTGGAGAAGGAAAAGAGTGAGCTGAAGATGGAGATCAATGACCTTGCTAG TAACATGGAGACTGTCTCCAAAGCCAAGGCAAACTTTGAGAAAATGTGCCGCACCCTAGAGGACCAGCTT AGTGAAATAAAAACAAAGGAAGAAGAGCAGCAACGCTTAATAAATGAGTTGTCAGCCCAGAAGGCACGTT TACACACAGAATCAGGTGAGTTTTCACGACAGCTAGATGAAAAAGATGCTATGGTTTCTCAGCTATCCCG AGGCAAACAAGCATTTACACAACAGATTGAAGAATTAAAGAGGCAGCTAGAAGAGGAGACTAAGGCCAAG AGCACTCTGGCCCATGCCCTGCAGTCAGCCCGCCATGACTGTGACCTGCTGCGGGAACAGTATGAGGAGG AGCAGGAAGCCAAGGCTGAGCTGCAGAGGGGAATGTCCAAGGCCAACAGTGAGGTTGCCCAGTGGAGGAC CAAGTACGAGACGGACGCCATCCAGCGCACAGAGGAGCTGGAGGAGGCCAAGAAGAAGCTAGCCCAGCGT CTGCAGGATGCAGAAGAACATGTAGAAGCTGTGAATTCCAAATGTGCTTCTCTTGAAAAGACAAAGCAGA GGCTACAGAATGAAGTAGAGGACCTCATGATTGATGTGGAACGATCTAATGCTGCCTGCATAGCTCTCGA TAAGAAGCAAAGAAACTTTGACAAGGTTCTGGCAGAATGGAAACAGAAGTATGAGGAAACTCAGGCTGAA CTTGAGGCCTCCCAGAAGGAGTCGCGTTCTCTCAGCACTGAGCTGTTCAAGGTGAAGAATGCCTACGAGG AATCCCTGGATCATCTTGAAACTCTAAAGCGAGAGAATAAGAACTTACAACAGGAGATTTCTGACCTGAC AGAGCAAATTGCAGAGGGTGGAAAGCATATCCATGAACTGGAGAAAGTAAAGAAACAACTTGATCATGAG AAGAGTGAACTACAGACTTCCCTAGAGGAAGCAGAGGCATCTCTTGAGCATGAAGAAGGCAAAATTCTTC GCATTCAACTTGAGCTAAATCAGGTGAAATCTGAGATTGACCGAAAAATTGCTGAAAAAGATGAAGAACT CGATCAGCTAAAGAGGAACCATCTCAGAGTTGTGGAGTCAATGCAGAGTACACTGGATGCTGAGATCAGG AGCAGAAATGATGCTCTGAGGATCAAGAAGAAGATGGAGGGAGATCTTAATGAAATGGAAATCCAGCTGA ACCATGCCAACCGCCAGGCTGCTGAGGCACTAAGGAATCTTAGAAACACACAAGGAATACTGAAGGACAC TCAGCTACATTTGGATGATGCCATCAGAGGCCAAGATGACCTTAAGGAACAATTGGCAATGGTTGAGCGC AGAGCTAACCTGATGCAGGCTGAAGTTGAAGAGCTCAGGGCATCCCTGGAACGGACTGAGAGAGGCAGGA AAATGGCAGAGCAAGAGCTTCTGGATGCCAGTGAACGTGTGCAACTTCTGCACACTCAGAACACCAGCCT GATCAACACCAAGAAGAAGCTGGAAACAGACATTTCCCAAATCCAGGGAGAGATGGAGGACATCGTCCAG GAAGCCCGCAATGCAGAGGAGAAGGCCAAGAAGGCCATCACTGATGCTGCCATGATGGCTGAGGAGCTGA AGAAGGAACAGGACACCAGCGCCCACCTGGAGCGGATGAAGAAGAACATGGAGCAGACCGTGAAGGATCT GCAGCTCCGTCTGGGTGAGGCTGAGCAGCTGGCGCTGAAGGGTGGGAAGAAGCAGATCCAGAAACTGGAG GCCAGGGTGAGAGAGCTTGAAAGTGAGGTGGAAAGTGAACAGAAGCACAATGTTGAGGCTGTCAAGGGTC TTCGCAAACATGAGAGAAGAGTGAAGGAACTCACTTACCAGACTGAGGAGGACCGCAAGAATATTCTCAG GCTGCAGGACTTGGTGGACAAATTGCAAACCAAAGTCAAAGCTTACAAGAGACAAGCTGAAGAGGCTGAG GAACAATCCAATGTCAACCTTGCCAAGTTCCGCAAGCTCCAGCACGAGCTGGAGGAGGCCGAGGAACGGG CTGACATTGCTGAGTCCCAAGTCAACAAGCTGAGAGTGAAGAGTCGGGAGGTTCACACAAAAGTCATAAG TGAAGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG220101 representing NM_017533
Red=Cloning site Green=Tags(s) MSSDSEMAIFGEAAPFLRKSEKERIEAQNKPFDAKTSVFVVDPKESYVKAIVQSREGGKVTAKTEAGATV TVKEDQVFSMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPEVV TAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEP ASGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVTFQL KAERSYHIFYQILSNKKPELIEMLLITTNPYDFAFVSQGEITVPSIDDQEELMATDSAVDILGFTADEKV AIYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKAAYLTSLNSADLLKSLCYPRVKVGNEFVTKGQTV QQVYNAVGALAKAIYEKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQ FFNHHMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYEQHL GKSNNFQKPKPAKGKPEAHFSLVHYAGTVDYNIAGWLDKNKDPLNETVVGLYQKSAMKTLAFLFSGAQTA EAEGGGGKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCN GVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLGSIEIDHTQYKFGHTKVFFK AGLLGTLEEMRDEKLAQLITRTQAICRGFLMRVEFRKMMERRESIFCIQYNIRAFMNVKHWPWMKLYFKI KPLLKSAETEKEMANMKEEFEKTKEELAKTEAKRKELEEKMVTLMQEKNDLQLQVQAEADALADAEERCD QLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVK NLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQMEEDKVNTLTKAKTKLEQQVDDLEGSLEQEKKLCM DLERAKRKLEGDLKLAQESTMDTENDKQQLNEKLKKKEFEMSNLQGKIEDEQALAMQLQKKIKELQARIE ELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIELNKKREAEFQKMRRDLEESTLQHE ATAAALRKKHADSVAELGKQIDSLQRVKQKLEKEKSELKMEINDLASNMETVSKAKANFEKMCRTLEDQL SEIKTKEEEQQRLINELSAQKARLHTESGEFSRQLDEKDAMVSQLSRGKQAFTQQIEELKRQLEEETKAK STLAHALQSARHDCDLLREQYEEEQEAKAELQRGMSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQR LQDAEEHVEAVNSKCASLEKTKQRLQNEVEDLMIDVERSNAACIALDKKQRNFDKVLAEWKQKYEETQAE LEASQKESRSLSTELFKVKNAYEESLDHLETLKRENKNLQQEISDLTEQIAEGGKHIHELEKVKKQLDHE KSELQTSLEEAEASLEHEEGKILRIQLELNQVKSEIDRKIAEKDEELDQLKRNHLRVVESMQSTLDAEIR SRNDALRIKKKMEGDLNEMEIQLNHANRQAAEALRNLRNTQGILKDTQLHLDDAIRGQDDLKEQLAMVER RANLMQAEVEELRASLERTERGRKMAEQELLDASERVQLLHTQNTSLINTKKKLETDISQIQGEMEDIVQ EARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTVKDLQLRLGEAEQLALKGGKKQIQKLE ARVRELESEVESEQKHNVEAVKGLRKHERRVKELTYQTEEDRKNILRLQDLVDKLQTKVKAYKRQAEEAE EQSNVNLAKFRKLQHELEEAEERADIAESQVNKLRVKSREVHTKVISEE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017533 |
| ORF Size | 5817 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017533.1, NP_060003.1 |
| RefSeq Size | 6016 bp |
| RefSeq ORF | 5820 bp |
| Locus ID | 4622 |
| Cytogenetics | 17p13.1 |
| Protein Pathways | Tight junction, Viral myocarditis |
| Gene Summary | '' |
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