Myosin (MYH4) (NM_017533) Human Untagged Clone

CAT#: SC304475

MYH4 (untagged)-Human myosin, heavy chain 4, skeletal muscle (MYH4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MYH4
• Synonyms: MYH2B; MyHC-2B; MyHC-IIb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_017533, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGTTCTGACTCTGAGATGGCCATTTTTGGGGAGGCTGCTCCTTTCCTCCGAAAGTCTGAAAAGGAGC
  GAATTGAAGCTCAGAACAAGCCTTTTGATGCCAAGACATCAGTCTTTGTGGTGGACCCTAAGGAGTCCTA
  CGTGAAAGCAATAGTGCAGAGCAGGGAAGGGGGGAAGGTGACAGCCAAGACCGAAGCTGGAGCTACTGTA
  ACTGTGAAAGAAGACCAAGTCTTCTCCATGAACCCTCCCAAATATGACAAGATCGAGGACATGGCCATGA
  TGACTCACCTGCATGAGCCTGCTGTGCTGTATAACCTCAAAGAGCGTTACGCAGCCTGGATGATCTACAC
  CTACTCGGGCCTCTTCTGTGTCACCGTCAACCCCTACAAGTGGCTGCCGGTGTACAACCCTGAGGTGGTG
  ACAGCCTACCGAGGCAAAAAGCGCCAGGAGGCCCCACCCCATATCTTCTCCATCTCTGACAATGCCTATC
  AGTTCATGCTAACTGATCGTGAAAACCAGTCAATCTTGATTACTGGAGAATCTGGTGCAGGGAAGACTGT
  GAACACGAAGCGTGTCATCCAGTACTTTGCAACAATTGCAGTTACTGGAGAGAAGAAAAAAGAGGAACCT
  GCCTCTGGCAAAATGCAGGGGACCCTTGAAGATCAAATCATCAGTGCTAACCCCCTACTGGAAGCCTTCG
  GCAATGCCAAGACCGTGAGGAATGACAACTCCTCTCGCTTTGGTAAATTCATCAGGATCCATTTTGGTGC
  CACAGGCAAACTGGCTTCTGCAGATATTGAAACATATCTGCTAGAGAAGTCCCGAGTTACTTTTCAGCTA
  AAGGCTGAAAGAAGCTACCACATATTTTATCAAATCCTGTCCAATAAGAAACCAGAGCTCATTGAAATGC
  TTCTGATCACCACCAACCCATATGACTTCGCATTTGTCAGCCAAGGGGAAATTACTGTGCCCAGCATTGA
  TGACCAGGAAGAGCTGATGGCCACAGATAGTGCTGTGGACATCCTGGGTTTCACTGCTGATGAAAAGGTG
  GCCATTTACAAGCTCACTGGAGCCGTGATGCATTATGGGAACATGAAATTCAAGCAAAAGCAAAGGGAAG
  AGCAGGCAGAGCCAGATGGCACGGAAGTTGCTGACAAAGCTGCTTATCTGACAAGTCTGAACTCTGCTGA
  CCTGCTCAAATCTCTCTGCTATCCCAGAGTCAAGGTCGGCAATGAGTTCGTAACCAAAGGCCAGACTGTG
  CAGCAGGTGTACAATGCAGTGGGTGCTCTGGCCAAAGCCATCTACGAGAAGATGTTCCTGTGGATGGTCA
  CCCGCATCAACCAGCAGCTGGACACCAAGCAGCCCAGGCAGTACTTCATCGGGGTCTTGGACATTGCTGG
  CTTTGAGATCTTTGATTTCAACAGCCTGGAGCAGCTGTGCATCAACTTCACCAACGAGAAACTGCAACAG
  TTTTTCAACCACCACATGTTCGTGCTGGAGCAGGAAGAGTACAAGAAGGAAGGCATCGAGTGGGAGTTCA
  TTGACTTCGGGATGGACCTGGCTGCCTGCATCGAGCTCATCGAGAAGCCTATGGGCATCTTCTCCATCCT
  AGAAGAGGAGTGCATGTTCCCCAAGGCAACAGACACCTCCTTCAAGAACAAGCTGTATGAACAACATCTT
  GGAAAATCCAACAACTTCCAGAAGCCCAAGCCTGCCAAAGGCAAGCCTGAGGCTCACTTCTCACTGGTGC
  ACTATGCCGGCACCGTGGACTACAACATCGCCGGCTGGCTGGACAAAAACAAGGACCCCCTGAATGAGAC
  TGTGGTGGGGCTGTACCAGAAGTCTGCAATGAAGACTCTGGCTTTCCTCTTCTCTGGGGCACAAACTGCT
  GAAGCAGAGGGTGGTGGTGGAAAGAAAGGTGGCAAAAAGAAGGGTTCTTCTTTCCAGACAGTGTCAGCTC
  TTTTCAGGGAGAATTTGAATAAGCTGATGACCAACTTGAGGAGCACTCACCCCCACTTTGTGCGGTGCAT
  CATCCCCAATGAAACTAAAACTCCTGGTGCCATGGAGCATGAGCTTGTCCTGCATCAGCTGAGGTGTAAC
  GGTGTGCTGGAAGGCATCCGCATCTGCAGGAAAGGCTTCCCAAGCAGAATCCTTTATGCAGACTTCAAAC
  AGAGATACAAGGTTCTAAATGCGAGTGCTATCCCAGAGGGTCAGTTCATTGACAGCAAGAAGGCTTCTGA
  GAAACTTCTAGGGTCTATTGAAATTGACCACACCCAGTACAAATTCGGTCATACCAAGGTTTTCTTCAAA
  GCTGGCCTGCTGGGAACTCTAGAGGAAATGCGAGATGAAAAGCTAGCTCAACTCATCACGCGCACTCAAG
  CCATATGCAGAGGGTTCCTGATGAGAGTGGAGTTCAGAAAGATGATGGAGAGGAGAGAGTCCATCTTCTG
  CATTCAGTACAACATCCGTGCTTTCATGAATGTGAAGCACTGGCCCTGGATGAAGCTGTATTTCAAGATC
  AAGCCCCTCCTCAAGAGTGCAGAGACAGAGAAGGAGATGGCCAACATGAAGGAAGAATTTGAGAAAACCA
  AAGAAGAGCTGGCTAAGACAGAGGCAAAAAGGAAAGAACTAGAAGAAAAGATGGTGACGCTAATGCAAGA
  GAAAAATGACTTACAACTCCAAGTTCAAGCTGAAGCAGATGCCTTGGCTGATGCAGAGGAAAGATGTGAT
  CAGTTGATTAAAACCAAAATCCAACTTGAGGCCAAAATCAAAGAGGTAACTGAAAGAGCTGAGGATGAGG
  AAGAGATCAATGCTGAGCTGACAGCCAAGAAGAGGAAACTGGAGGATGAATGTTCAGAGCTCAAGAAAGA
  CATTGATGACCTTGAGCTGACACTGGCCAAGGTTGAGAAGGAGAAACATGCCACAGAGAACAAGGTGAAA
  AACCTCACAGAAGAGATGGCAGGTCTGGATGAAACCATTGCTAAGCTGACCAAGGAGAAGAAGGCTCTCC
  AGGAGGCCCACCAGCAGACCCTGGATGACCTGCAGATGGAGGAGGACAAAGTCAACACCCTGACCAAAGC
  TAAAACCAAGCTAGAACAGCAAGTGGACGATCTTGAAGGATCTCTGGAACAAGAAAAGAAACTTTGCATG
  GACTTAGAAAGAGCCAAGAGAAAACTGGAGGGTGACCTAAAATTGGCCCAAGAATCCACAATGGATACAG
  AAAATGACAAACAGCAACTTAATGAGAAACTCAAAAAGAAAGAGTTTGAAATGAGCAATCTGCAAGGCAA
  GATTGAAGATGAACAAGCCCTTGCAATACAGCTACAAAAGAAGATCAAAGAATTACAGGCCCGCATTGAG
  GAGCTGGAGGAGGAAATCGAGGCAGAGCGGGCCTCCCGGGCCAAAGCAGAGAAGCAGCGCTCTGACCTCT
  CCCGGGAGCTGGAGGAGATCAGTGAGAGGCTGGAAGAAGCCGGTGGGGCCACTTCAGCCCAGATTGAGAT
  GAACAAGAAGCGGGAGGCTGAGTTCCAGAAAATGCGCAGGGACCTGGAAGAGTCCACCCTGCAGCACGAA
  GCCACGGCAGCTGCTCTTCGGAAGAAGCACGCAGATAGTGTGGCTGAGCTTGGGGAGCAGATTGACAGCC
  TTCAGCGGGTCAAGCAGAAGCTGGAGAAGGAAAAGAGTGAGCTGAAGATGGAGATCAATGACCTTGCTAG
  TAACATGGAGACTGTCTCCAAAGCCAAGGCAAACTTTGAGAAAATGTGCCGCACCCTAGAGGACCAGCTT
  AGTGAAATAAAAACAAAGGAAGAAGAGCAACAACGCTTAATAAATGAGTTGTCAGCCCAGAAGGCACGTT
  TACACACAGAATCAGGTGAGTTTTCACGACAGCTAGATGAAAAAGATGCTATGGTTTCTCAGCTATCCCG
  AGGCAAACAAGCATTTACACAACAGATTGAAGAATTAAAGAGGCAGCTAGAAGAGGAGACTAAGGCCAAG
  AGCACTCTGGCCCATGCCCTGCAGTCAGCCCGCCATGACTGTGACCTGCTGCGGGAACAGTATGAGGAGG
  AGCAGGAAGCCAAGGCTGAGCTGCAGAGGGGAATGTCCAAGGCCAACAGTGAGGTTGCCCAGTGGAGGAC
  CAAGTACGAGACGGACGCCATCCAGCGCACAGAGGAGCTGGAGGAGGCCAAGAAGAAGCTAGCCCAGCGT
  CTGCAGGATGCAGAAGAACATGTAGAAGCTGTGAATTCCAAATGTGCTTCTCTTGAAAAGACAAAGCAGA
  GGCTACAGAATGAAGTAGAGGACCTCATGATTGATGTGGAACGATCTAATGCTGCCTGCATAGCTCTCGA
  TAAGAAGCAAAGAAACTTTGACAAGGTTCTGGCAGAATGGAAACAGAAGTATGAGGAAACTCAGGCTGAA
  CTTGAGGCCTCCCAGAAGGAGTCGCGTTCTCTCAGCACTGAGCTGTTCAAGGTGAAGAATGCCTACGAGG
  AATCCCTGGATCATCTTGAAACTCTAAAGCGAGAGAATAAGAACTTACAACAGGAGATTTCTGACCTGAC
  AGAGCAAATTGCAGAGGGTGGAAAGCATATCCATGAACTGGAGAAAGTAAAGAAACAACTTGATCATGAG
  AAGAGTGAACTACAGACTTCCCTAGAGGAAGCAGAGGCATCTCTTGAGCATGAAGAAGGCAAAATTCTTC
  GCATTCAACTTGAGCTAAATCAGGTGAAATCTGAGATTGACCGAAAAATTGCTGAAAAAGATGAAGAACT
  CGATCAGCTAAAGAGGAACCATCTCAGAGTTGTGGAGTCAATGCAGAGTACACTGGATGCTGAGATCAGG
  AGCAGAAATGATGCTCTGAGGATCAAGAAGAAGATGGAGGGAGATCTTAATGAAATGGAAATCCAGCTGA
  ACCATGCCAACCGCCAGGCTGCTGAGGCACTAAGGAATCTTAGAAACACACAAGGAATACTGAAGGACAC
  TCAGCTACATTTGGATGATGCCATCAGAGGCCAAGATGACCTTAAGGAACAACTGGCAATGGTTGAGCGC
  AGAGCTAACCTGATGCAGGCTGAAGTTGAAGAGCTCAGGGCATCCCTGGAACGGACTGAGAGAGGCAGGA
  AAATGGCAGAGCAAGAGCTTCTGGATGCCAGTGAACGTGTGCAACTTCTGCACACTCAGAACACCAGCCT
  GATCAACACCAAGAAGAAGCTGGAAACAGACATTTCCCAAATCCAGGGAGAGATGGAGGACATCGTCCAG
  GAAGCCCGCAATGCAGAGGAGAAGGCCAAGAAGGCCATCACTGATGCTGCCATGATGGCTGAGGAGCTGA
  AGAAGGAACAGGACACCAGCGCCCACCTGGAGCGGATGAAGAAGAACATGGAGCAGACCGTGAAGGATCT
  GCAGCTCCGTCTGGATGAGGCTGAGCAGCTGGCGCTGAAGGGTGGGAAGAAGCAGATCCAGAAACTGGAG
  GCCAGGGTGAGAGAGCTTGAAAGTGAGGTGGAAAGTGAACAGAAGCACAATGTTGAGGCTGTCAAGGGTC
  TTCGCAAACATGAGAGAAGAGTGAAGGAACTCACTTACCAGACTGAGGAGGACCGCAAGAATATTCTCAG
  GCTGCAGGACTTGGTGGACAAATTGCAAACCAAAGTCAAAGCTTACAAGAGACAAGCTGAAGAGGCTGAG
  GAACAATCCAATGTCAACCTTGCCAAGTTCCGCAAGCTCCAGCACGAGCTGGAGGAGGCCAAGGAACGGG
  CTGACATTGCTGAGTCCCAAGTCAACAAGCTGAGAGTGAAGAGTCGGGAGGTTCACACAAAAGTCATAAG
  TGAAGAGTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_017533
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017533.2, NP_060003.2
• RefSeq Size: 6016 bp
• RefSeq ORF: 5820 bp
• Locus ID: 4622
• Cytogenetics: 17p13.1
• Protein Pathways: Tight junction, Viral myocarditis
• Gene Summary: ''