MAP3K13 (NM_004721) Human Tagged ORF Clone

CAT#: RG220337

MAP3K13 (GFP-tagged) - Human mitogen-activated protein kinase kinase kinase 13 (MAP3K13), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MAP3K13
• Synonyms: LZK; MEKK13; MLK
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG220337 representing NM_004721
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAACCTTCAGGAGCACCTGAGCTGCTCCTCTTCTCCACACTTACCCTTCAGTGAAAGCAAAACCT
  TCAATGGACTACAAGATGAGCTCACAGCTATGGGGAACCACCCTTCTCCCAAGCTGCTCGAGGACCAGCA
  GGAAAAGGGGATGGTACGAACAGAGCTAATCGAGAGCGTGCACAGCCCCGTCACCACAACAGTGTTGACG
  AGCGTAAGTGAGGATTCCAGGGACCAGTTTGAGAACAGCGTTCTTCAGCTAAGGGAACACGATGAATCAG
  AGACGGCGGTGTCTCAGGGGAACAGCAACACGGTGGACGGAGAGAGCACAAGCGGAACTGAAGACATAAA
  GATTCAGTTCAGCAGGTCAGGCAGTGGCAGTGGTGGGTTTCTTGAAGGACTATTTGGATGCTTAAGGCCT
  GTATGGAATATCATTGGGAAGGCATATTCCACTGATTACAAATTGCAGCAGCAAGATACTTGGGAAGTGC
  CATTTGAGGAGATCTCAGAGCTGCAGTGGCTGGGTAGTGGAGCCCAAGGAGCGGTCTTCTTGGGCAAGTT
  CCGGGCGGAAGAGGTGGCCATCAAGAAAGTGAGAGAACAGAATGAGACGGATATCAAGCATTTGAGGAAG
  TTGAAGCACCCTAACATCATCGCATTCAAGGGTGTTTGTACTCAGGCCCCATGTTATTGTATTATCATGG
  AATACTGTGCCCATGGACAACTCTACGAGGTCTTACGAGCTGGCAGGAAGATCACACCTCGATTGCTAGT
  AGACTGGTCCACAGGAATTGCAAGTGGAATGAATTATTTGCACCTCCATAAAATTATTCATCGTGATCTC
  AAATCACCTAATGTTTTAGTGACCCACACAGATGCGGTAAAAATTTCAGATTTTGGTACATCTAAGGAAC
  TCAGTGACAAAAGTACCAAGATGTCATTTGCTGGCACGGTCGCATGGATGGCGCCAGAGGTGATACGGAA
  TGAACCTGTCTCTGAAAAAGTTGATATATGGTCTTTTGGAGTGGTGCTTTGGGAGCTGCTGACAGGAGAG
  ATCCCTTACAAAGATGTAGATTCTTCAGCCATTATCTGGGGTGTTGGAAGCAACAGCCTCCACCTTCCAG
  TTCCTTCCACTTGCCCTGATGGATTCAAAATCCTTATGAAACAGACGTGGCAGAGTAAACCTCGAAACCG
  ACCTTCTTTTCGGCAGACACTCATGCATTTAGACATTGCCTCTGCAGATGTACTTGCCACCCCACAAGAA
  ACTTACTTCAAGTCTCAGGCTGAATGGAGAGAAGAAGTGAAAAAACATTTTGAGAAGATCAAAAGTGAAG
  GAACTTGTATACACCGGTTAGATGAAGAACTGATTCGAAGGCGCAGAGAAGAGCTCAGGCATGCGCTGGA
  TATTCGTGAACACTATGAGCGGAAGCTTGAGCGGGCGAATAATTTATACATGGAATTGAGTGCCATCATG
  CTGCAGCTAGAAATGCGGGAGAAGGAGCTCATTAAGCGTGAGCAAGCAGTGGAAAAGAAGTATCCTGGGA
  CCTACAAACGACACCCTGTTCGTCCTATCATCCATCCCAATGCCATGGAGAAACTCATGAAAAGGAAAGG
  AGTGCCTCACAAATCTGGGATGCAGACCAAACGGCCAGACTTGTTGAGATCAGAAGGGATCCCCACCACA
  GAAGTGGCTCCCACTGCATCCCCTTTGTCCGGAAGTCCCAAAATGTCCACTTCTAGCAGCAAGAGCCGAT
  ATCGAAGCAAACCACGCCACCGCCGAGGGAATAGCAGAGGCAGCCATAGTGACTTTGCCGCAATCTTGAA
  AAACCAGCCAGCCCAGGAAAATTCACCCCATCCCACTTACCTGCACCAAGCTCAATCCCAATACCCTTCT
  CTTCATCACCATAATTCTCTGCAGCAGCAATACCAGCAGCCCCCTCCTGCCATGTCCCAGAGTCACCATC
  CCAGACTCAATATGCACGGACAGGACATAGCAACCTGCGCCAACAACCTGAGGTATTTCGGCCCAGCAGC
  AGCCCTGCGGAGCCCACTCAGCAACCATGCTCAGAGACAGCTGCCCGGCTCGAGCCCTGACCTCATCTCC
  ACAGCCATGGCTGCAGACTGCTGGAGAAGTTCTGAGCCTGACAAGGGCCAAGCTGGTCCCTGGGGCTGTT
  GCCAGGCTGACGCTTATGACCCCTGCCTTCAGTGCAGGCCAGAACAGTATGGGTCCTTAGACATACCCTC
  TGCTGAGCCAGTGGGGAGGAGCCCTGACCTTTCCAAGTCACCAGCACATAATCCTCTCTTGGAAAACGCC
  CAGAGTTCTGAGAAAACGGAAGAAAATGAATTCAGCGGCTGTAGGTCTGAGTCATCCCTCGGCACCTCTC
  ATCTCGGCACCCCTCCAGCGCTACCTCGAAAAACAAGGCCTCTGCAGAAGAGTGGAGATGACTCCTCAGA
  AGAGGAAGAAGGGGAAGTAGATAGTGAAGTTGAATTTCCACGAAGACAGAGGCCCCATCGCTGTATCAGC
  AGCTGCCAGTCATATTCAACCTTTAGCTCTGAGAATTTCTCTGTGTCTGATGGAGAAGAGGGAAATACCA
  GTGACCACTCAAACAGTCCTGATGAGTTAGCTGATAAACTTGAAGACCGCTTGGCAGAGAAGCTAGACGA
  CCTGCTGTCCCAGACGCCAGAGATTCCCATTGACATATCCTCACACTCGGATGGGCTCTCTGACAAGGAG
  TGTGCCGTGCGCCGTGTGAAGACTCAGATGTCTCTGGGCAAGCTGTGTGTGGAGGAACGTGGCTATGAGA
  ACCCCATGCAGTTTGAAGAATCGGACTGTGACTCTTCAGATGGGGAGTGTTCTGATGCCACAGTTAGGAC
  CAATAAACACTACAGCTCTGCTACCTGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG220337 representing NM_004721
  Red=Cloning site Green=Tags(s)
  
  MANLQEHLSCSSSPHLPFSESKTFNGLQDELTAMGNHPSPKLLEDQQEKGMVRTELIESVHSPVTTTVLT
  SVSEDSRDQFENSVLQLREHDESETAVSQGNSNTVDGESTSGTEDIKIQFSRSGSGSGGFLEGLFGCLRP
  VWNIIGKAYSTDYKLQQQDTWEVPFEEISELQWLGSGAQGAVFLGKFRAEEVAIKKVREQNETDIKHLRK
  LKHPNIIAFKGVCTQAPCYCIIMEYCAHGQLYEVLRAGRKITPRLLVDWSTGIASGMNYLHLHKIIHRDL
  KSPNVLVTHTDAVKISDFGTSKELSDKSTKMSFAGTVAWMAPEVIRNEPVSEKVDIWSFGVVLWELLTGE
  IPYKDVDSSAIIWGVGSNSLHLPVPSTCPDGFKILMKQTWQSKPRNRPSFRQTLMHLDIASADVLATPQE
  TYFKSQAEWREEVKKHFEKIKSEGTCIHRLDEELIRRRREELRHALDIREHYERKLERANNLYMELSAIM
  LQLEMREKELIKREQAVEKKYPGTYKRHPVRPIIHPNAMEKLMKRKGVPHKSGMQTKRPDLLRSEGIPTT
  EVAPTASPLSGSPKMSTSSSKSRYRSKPRHRRGNSRGSHSDFAAILKNQPAQENSPHPTYLHQAQSQYPS
  LHHHNSLQQQYQQPPPAMSQSHHPRLNMHGQDIATCANNLRYFGPAAALRSPLSNHAQRQLPGSSPDLIS
  TAMAADCWRSSEPDKGQAGPWGCCQADAYDPCLQCRPEQYGSLDIPSAEPVGRSPDLSKSPAHNPLLENA
  QSSEKTEENEFSGCRSESSLGTSHLGTPPALPRKTRPLQKSGDDSSEEEEGEVDSEVEFPRRQRPHRCIS
  SCQSYSTFSSENFSVSDGEEGNTSDHSNSPDELADKLEDRLAEKLDDLLSQTPEIPIDISSHSDGLSDKE
  CAVRRVKTQMSLGKLCVEERGYENPMQFEESDCDSSDGECSDATVRTNKHYSSATW
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004721
• ORF Size: 2898 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_004721.3, NP_004712.1
• RefSeq Size: 3568
• RefSeq ORF: 2901
• Locus ID: 9175
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors
• Protein Pathways: MAPK signaling pathway
• Gene Summary: The protein encoded by this gene is a member of serine/threonine protein kinase family. This kinase contains a dual leucine-zipper motif, and has been shown to form dimers/oligomers through its leucine-zipper motif. This kinase can phosphorylate and activate MAPK8/JNK, MAP2K7/MKK7, which suggests a role in the JNK signaling pathway. [provided by RefSeq, Jul 2008]