MAP3K13 (NM_004721) Human Untagged Clone

CAT#: SC323617

MAP3K13 (untagged)-Kinase deficient mutant (K195M) of Human mitogen-activated protein kinase kinase kinase 13 (MAP3K13)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAP3K13
• Synonyms: LZK; MEKK13; MLK
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323617 sequence for NM_004721 edited (data generated by NextGen Sequencing)
  ATGGCCAACCTTCAGGAGCACCTGAGCTGCTCCTCTTCTCCACACTTACCCTTCAGTGAA
  AGCAAAACCTTCAATGGACTACAAGATGAGCTCACAGCTATGGGGAACCACCCTTCTCCC
  AAGCTGCTCGAGGACCAGCAGGAAAAGGGGATGGTACGAACAGAGCTAATCGAGAGCGTG
  CACAGCCCCGTCACCACAACAGTGTTGACGAGCGTAAGTGAGGATTCCAGGGACCAGTTT
  GAGAACAGCGTTCTTCAGCTAAGGGAACACGATGAATCAGAGACGGCGGTGTCTCAGGGG
  AACAGCAACACGGTGGACGGAGAGAGCACAAGCGGAACTGAAGACATAAAGATTCAGTTC
  AGCAGGTCAGGCAGTGGCAGTGGTGGGTTTCTTGAAGGACTATTTGGATGCTTAAGGCCT
  GTATGGAATATCATTGGGAAGGCATATTCCACTGATTACAAATTGCAGCAGCAAGATACT
  TGGGAAGTGCCATTTGAGGAGATCTCAGAGCTGCAGTGGCTGGGTAGTGGAGCCCAAGGA
  GCGGTCTTCTTGGGCAAGTTCCGGGCGGAAGAGGTGGCCATCATGAAAGTGAGAGAACAG
  AATGAGACGGATATCAAGCATTTGAGGAAGTTGAAGCACCCTAACATCATCGCATTCAAG
  GGTGTTTGTACTCAGGCCCCATGTTATTGTATTATCATGGAATACTGTGCCCATGGACAA
  CTCTACGAGGTCTTACGAGCTGGCAGGAAGATCACACCTCGATTGCTAGTAGACTGGTCC
  ACAGGAATTGCAAGTGGAATGAATTATTTGCACCTCCATAAAATTATTCATCGTGATCTC
  AAATCACCTAATGTTTTAGTGACCCACACAGATGCGGTAAAAATTTCAGATTTTGGTACA
  TCTAAGGAACTCAGTGACAAAAGTACCAAGATGTCATTTGCTGGCACGGTCGCATGGATG
  GCGCCAGAGGTGATACGGAATGAACCTGTCTCTGAAAAAGTTGATATATGGTCTTTTGGA
  GTGGTGCTTTGGGAGCTGCTGACAGGAGAGATCCCTTACAAAGATGTAGATTCTTCAGCC
  ATTATCTGGGGTGTTGGAAGCAACAGCCTCCACCTTCCAGTTCCTTCCACTTGCCCTGAT
  GGATTCAAAATCCTTATGAAACAGACGTGGCAGAGTAAACCTCGAAACCGACCTTCTTTT
  CGGCAGACACTCATGCATTTAGACATTGCCTCTGCAGATGTACTTGCCACCCCACAAGAA
  ACTTACTTCAAGTCTCAGGCTGAATGGAGAGAAGAAGTGAAAAAACATTTTGAGAAGATC
  AAAAGTGAAGGAACTTGTATACACCGGTTAGATGAAGAACTGATTCGAAGGCGCAGAGAA
  GAGCTCAGGCATGCGCTGGATATTCGTGAACACTATGAGCGGAAGCTTGAGCGGGCGAAT
  AATTTATACATGGAATTGAGTGCCATCATGCTGCAGCTAGAAATGCGGGAGAAGGAGCTC
  ATTAAGCGTGAGCAAGCAGTGGAAAAGAAGTATCCTGGGACCTACAAACGACACCCTGTT
  CGTCCTATCATCCATCCCAATGCCATGGAGAAACTCATGAAAAGGAAAGGAGTGCCTCAC
  AAATCTGGGATGCAGACCAAACGGCCAGACTTGTTGAGATCAGAAGGGATCCCCACCACA
  GAAGTGGCTCCCACTGCATCCCCTTTGTCCGGAAGTCCCAAAATGTCCACTTCTAGCAGC
  AAGAGCCGATATCGAAGCAAACCACGCCACCGCCGAGGGAATAGCAGAGGCAGCCATAGT
  GACTTTGCCGCAATCTTGAAAAACCAGCCAGCCCAGGAAAATTCACCCCATCCCACTTAC
  CTGCACCAAGCTCAATCCCAATACCCTTCTCTTCATCACCATAATTCTCTGCAGCAGCAA
  TACCAGCAGCCCCCTCCTGCCATGTCCCAGAGTCACCATCCCAGACTCAATATGCACGGA
  CAGGACATAGCAACCTGCGCCAACAACCTGAGGTATTTCGGCCCAGCAGCAGCCCTGCGG
  AGCCCACTCAGCAACCATGCTCAGAGACAGCTGCCCGGCTCGAGCCCTGACCTCATCTCC
  ACAGCCATGGCTGCAGACTGCTGGAGAAGTTCTGAGCCTGACAAGGGCCAAGCTGGTCCC
  TGGGGCTGTTGCCAGGCTGACGCTTATGACCCCTGCCTTCAGTGCAGGCCAGAACAGTAT
  GGGTCCTTAGACATACCCTCTGCTGAGCCAGTGGGGAGGAGCCCTGACCTTTCCAAGTCA
  CCAGCACATAATCCTCTCTTGGAAAACGCCCAGAGTTCTGAGAAAACGGAAGAAAATGAA
  TTCAGCGGCTGTAGGTCTGAGTCATCCCTCGGCACCTCTCATCTCGGCACCCCTCCAGCG
  CTACCTCGAAAAACAAGGCCTCTGCAGAAGAGTGGAGATGACTCCTCAGAAGAGGAAGAA
  GGGGAAGTAGATAGTGAAGTTGAATTTCCACGAAGACAGAGGCCCCATCGCTGTATCAGC
  AGCTGCCAGTCATATTCAACCTTTAGCTCTGAGAATTTCTCTGTGTCTGATGGAGAAGAG
  GGAAATACCAGTGACCACTCAAACAGTCCTGATGAGTTAGCTGATAAACTTGAAGACCGC
  TTGGCAGAGAAGCTAGACGACCTGCTGTCCCAGACGCCAGAGATTCCCATTGACATATCC
  TCACACTCGGATGGGCTCTCTGACAAGGAGTGTGCCGTGCGCCGTGTGAAGACTCAGATG
  TCTCTGGGCAAGCTGTGTGTGGAGGAACGTGGCTATGAGAACCCCATGCAGTTTGAAGAA
  TCGGACTGTGACTCTTCAGATGGGGAGTGTTCTGATGCCACAGTTAGGACCAATAAACAC
  TACAGCTCTGCTACCTGGTAA
  
  Clone variation with respect to NM_004721.4
  10 t=>c;584 a=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_004721 unedited
  ACGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAC
  CGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGATGGAACAGCATGTG
  TAGGAATTCTTCGTTGTTGTTTTGGAGCCCTCTCTTAAGTCAGAACTCTGTCCCAAAAATCTTCTGAGTG
  TCATCTCAGGACTTTGGTTATACTCATGGCACGATGGCCAACCTTCAGGAGCACCTGAGCTGCTCCTCTT
  CTCCACACTTACCCTTCAGTGAAAGCAAAACCTTCAATGGACTACAAAGATGAGCTCACAGCTATGGGGG
  AACCACCCCTTCTCCCAAGCTGCTCGAGGACCAGCAGGAAAAGGGGGATGGTACGAACAGGAGCTAATCG
  AGAGAGCGTGGCACAGCCCCGTCACCCCCAAACGTTGTTGACGAGCGTAGTTGAGGATTCCAGGGACCAG
  GTTTGAAAACCAGCGTCCTTCGGCTAAGGGAACACGAATAAATAAAAACCGGCGTGGCCTCAGGGAAACA
  GCACACACGTTGGACGGGAGAGACCACACGGGAACGAGAACCTTAGAGATTCATTCACACAGGTCAGGGC
  TGTGGCATGTGTGGTTTTTTAGAGAGACTATGGGTGCGCTTAGGCCCTGTAGGAATATCATTGAAGGCAT
  TTCCCTGTATACAATGTGCAGCACAGACTCTGAAGTCCTTTGAGATATTCTAACCCAGCGTGAAAGAAAA
  CCCAGAGACGCTCTTGGCAATTCGGCGAAAGTGCCCTTTGAATGGGAACCAAGTAGCCGAATCGCGTAGG
  GAGTTGACTCTACCTCGCTAAGGTGTTACTAGCCAGGTA
  
  Kinase domain sequence

  >SC323617 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  GAYTAGAGCTGCAGTGGCTGGGTAGTGGAGCCCAAGGAGCGGTCTTCTTGGGCAAGTTCCGGGCGGAAGA
  GGTGGCCATCATGAAAGTGAGAGAACAGAATGAGACGGATATCAAGCATTTGAGGAAGTTGAAGCACCCT
  AACATCATCGCATTCAAGGGTGTTTGTACTCAGGCCCCATGTTATTGTATTATCATGGAATACTGTGCCC
  ATGGACAACTCTACGAGGTCTTACGAGCTGGCAGGAAGATCACAC
  
• Restriction Sites: Please inquire
• ACCN: NM_004721
• ORF Size: 2901 bp
• Insert Size: 3600
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_004721.3, NP_004712.1
• RefSeq Size: 3568
• RefSeq ORF: 2901
• Locus ID: 9175
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors
• Protein Pathways: MAPK signaling pathway
• Gene Summary: The protein encoded by this gene is a member of serine/threonine protein kinase family. This kinase contains a dual leucine-zipper motif, and has been shown to form dimers/oligomers through its leucine-zipper motif. This kinase can phosphorylate and activate MAPK8/JNK, MAP2K7/MKK7, which suggests a role in the JNK signaling pathway. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longest transcript. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.