Phosphorylase B (PHKB) (NM_000293) Human Tagged ORF Clone

CAT#: RG220846

PHKB (GFP-tagged) - Human phosphorylase kinase, beta (PHKB), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PHKB
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG220846 representing NM_000293
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGGGCGGCGGGACTCACGGCAGAAGTGAGCTGGAAGGTCTTGGAGCGAAGAGCTCGGACCAAGC
  GCTCAGGCTCAGTTTATGAACCTCTTAAAAGCATTAATCTTCCAAGACCTGATAATGAAACTCTCTGGGA
  TAAGTTGGACCATTATTACAGAATTGTCAAGTCAACATTGCTGCTGTATCAAAGTCCAACTACCGGTCTC
  TTTCCCACTAAAACATGCGGTGGTGACCAGAAGGCCAAGATCCAGGACAGCCTATACTGCGCTGCTGGGG
  CCTGGGCTTTGGCTCTTGCATACAGGCGAATTGATGATGACAAGGGAAGGACCCATGAGCTGGAGCACTC
  AGCTATAAAATGCATGAGAGGAATTCTCTACTGCTATATGCGTCAGGCCGATAAGGTCCAGCAGTTTAAG
  CAGGATCCACGCCCAACAACATGTCTTCACTCTGTTTTCAATGTGCATACAGGAGATGAGTTGCTTTCCT
  ATGAGGAATATGGTCATCTTCAGATAAATGCAGTGTCACTTTATCTCCTTTACCTTGTGGAAATGATTTC
  CTCAGGACTCCAGATTATCTACAACACTGATGAGGTCTCTTTTATTCAAAACCTTGTATTTTGTGTGGAA
  AGAGTTTACCGTGTGCCTGACTTTGGTGTCTGGGAAAGAGGAAGCAAATATAATAATGGCAGCACAGAGC
  TACATTCGAGCTCGGTTGGTTTAGCAAAAGCAGCTCTAGAAGCAATTAATGGATTCAACCTTTTTGGCAA
  CCAGGGCTGTTCGTGGTCAGTTATATTTGTGGATCTCGATGCTCACAATCGCAACAGGCAAACTTTGTGC
  TCGCTGTTACCCAGAGAATCAAGATCACATAATACAGATGCTGCCCTGCTCCCCTGCATCAGTTATCCTG
  CATTTGCCCTGGATGATGAAGTTCTTTTTAGCCAGACACTTGATAAAGTGGTTAGAAAATTAAAAGGAAA
  ATATGGATTTAAACGTTTCTTGAGAGATGGGTATAGAACATCATTGGAAGATCCCAACAGATGCTACTAC
  AAGCCAGCTGAAATTAAGCTATTTGATGGCATTGAATGTGAATTTCCCATATTTTTCCTTTATATGATGA
  TTGATGGAGTTTTTAGAGGCAATCCTAAGCAAGTACAGGAATATCAGGATCTTTTGACTCCAGTACTTCA
  TCATACCACAGAAGGATATCCTGTTGTACCAAAGTACTATTATGTGCCAGCTGACTTTGTAGAATATGAA
  AAAAATAACCCTGGTAGTCAAAAACGATTTCCTAGCAACTGTGGCCGTGATGGAAAACTGTTTCTTTGGG
  GACAAGCACTTTATATCATCGCAAAACTCCTGGCTGATGAACTTATTAGTCCTAAAGACATTGATCCTGT
  CCAGCGCTATGTCCCACTAAAGGATCAACGTAACGTGAGCATGAGGTTTTCCAATCAGGGCCCACTGGAA
  AATGACTTGGTAGTTCATGTGGCACTTATAGCAGAAAGCCAAAGACTTCAAGTTTTTCTGAACACATATG
  GTATTCAAACTCAAACTCCTCAACAAGTAGAACCCATTCAGATATGGCCTCAGCAGGAGCTTGTGAAAGC
  TTATTTGCAGCTGGGTATCAATGAAAAGTTAGGACTCTCTGGAAGGCCAGACAGGCCCATTGGCTGCCTC
  GGGACATCAAAGATTTATCGCATTCTAGGAAAGACTGTGGTTTGTTACCCGATTATTTTCGACCTAAGTG
  ATTTCTACATGTCTCAGGATGTTTTCCTGCTGATAGATGACATAAAGAATGCGCTGCAGTTCATTAAACA
  ATATTGGAAAATGCATGGACGTCCACTTTTCCTTGTTCTCATCCGGGAAGACAATATAAGAGGTAGCCGG
  TTCAACCCCATATTAGATATGCTGGCAGCCCTTAAAAAAGGAATAATTGGAGGAGTCAAAGTTCATGTGG
  ATCGTCTACAGACACTAATATCTGGAGCTGTGGTAGAACAACTTGATTTCCTACGAATCAGTGACACAGA
  AGAGCTTCCAGAATTTAAGAGTTTTGAGGAACTAGAACCTCCCAAACATTCAAAAGTCAAACGGCAAAGC
  AGCACCCCTAGTGCTCCTGAACTGGGACAGCAGCCGGATGTCAACATTAGTGAATGGAAGGACAAACCCA
  CCCACGAAATTCTTCAAAAACTGAATGATTGCAGTTGTCTGGCTAGCCAAGCCATCCTGCTGGGTATACT
  GCTCAAAAGAGAAGGCCCCAACTTCATCACAAAGGAAGGTACCGTTTCTGATCACATTGAGAGAGTCTAT
  AGAAGAGCTGGCAGCCAAAAACTTTGGTTGGCGGTGCGCTACGGGGCTGCATTTACCCAGAAATTTTCTT
  CCTCTATAGCCCCACACATTACTACTTTTCTGGTACATGGGAAACAGGTAACTCTGGGTGCCTTTGGGCA
  TGAAGAAGAAGTTATCTCTAATCCTTTGTCTCCAAGAGTGATTCAAAACATCATCTATTATAAGTGTAAC
  ACCCATGATGAGAGGGAAGCGGTCATTCAGCAAGAACTGGTCATCCATATTGGCTGGATCATCTCCAATA
  ACCCTGAGTTATTCAGTGGCATGCTGAAAATACGAATCGGGTGGATCATCCATGCCATGGAGTATGAACT
  TCAGATCCGTGGCGGAGACAAGCCAGCCTTGGACTTGTATCAGCTGTCACCTAGTGAAGTTAAACAGCTT
  CTGCTGGATATTCTGCAGCCTCAACAGAATGGAAGATGTTGGCTGAACAGGCGTCAGATCGATGGGTCTT
  TGAATAGAACTCCCACCGGGTTCTATGACCGAGTGTGGCAGATTCTGGAGCGCACGCCCAATGGGATCAT
  TGTTGCTGGGAAGCATTTGCCTCAGCAACCAACCCTGTCAGATATGACCATGTATGAGATGAATTTCTCT
  CTCCTTGTTGAAGACACGTTGGGAAATATTGACCAGCCACAGTACAGACAGATCGTTGTAGAGTTACTTA
  TGGTTGTATCCATTGTACTGGAAAGAAACCCCGAGCTAGAATTTCAAGACAAAGTAGATCTAGACAGACT
  GGTCAAAGAAGCATTTAATGAATTTCAAAAAGATCAGAGTCGGCTAAAGGAAATTGAAAAACAAGATGAC
  ATGACTTCCTTTTACAACACTCCTCCCCTGGGAAAAAGAGGAACATGCAGCTATTTGACAAAGGCGGTGA
  TGAATCTGCTGCTGGAAGGAGAAGTCAAGCCAAACAATGATGACCCGTGTCTGATTAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG220846 representing NM_000293
  Red=Cloning site Green=Tags(s)
  
  MAGAAGLTAEVSWKVLERRARTKRSGSVYEPLKSINLPRPDNETLWDKLDHYYRIVKSTLLLYQSPTTGL
  FPTKTCGGDQKAKIQDSLYCAAGAWALALAYRRIDDDKGRTHELEHSAIKCMRGILYCYMRQADKVQQFK
  QDPRPTTCLHSVFNVHTGDELLSYEEYGHLQINAVSLYLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVE
  RVYRVPDFGVWERGSKYNNGSTELHSSSVGLAKAALEAINGFNLFGNQGCSWSVIFVDLDAHNRNRQTLC
  SLLPRESRSHNTDAALLPCISYPAFALDDEVLFSQTLDKVVRKLKGKYGFKRFLRDGYRTSLEDPNRCYY
  KPAEIKLFDGIECEFPIFFLYMMIDGVFRGNPKQVQEYQDLLTPVLHHTTEGYPVVPKYYYVPADFVEYE
  KNNPGSQKRFPSNCGRDGKLFLWGQALYIIAKLLADELISPKDIDPVQRYVPLKDQRNVSMRFSNQGPLE
  NDLVVHVALIAESQRLQVFLNTYGIQTQTPQQVEPIQIWPQQELVKAYLQLGINEKLGLSGRPDRPIGCL
  GTSKIYRILGKTVVCYPIIFDLSDFYMSQDVFLLIDDIKNALQFIKQYWKMHGRPLFLVLIREDNIRGSR
  FNPILDMLAALKKGIIGGVKVHVDRLQTLISGAVVEQLDFLRISDTEELPEFKSFEELEPPKHSKVKRQS
  STPSAPELGQQPDVNISEWKDKPTHEILQKLNDCSCLASQAILLGILLKREGPNFITKEGTVSDHIERVY
  RRAGSQKLWLAVRYGAAFTQKFSSSIAPHITTFLVHGKQVTLGAFGHEEEVISNPLSPRVIQNIIYYKCN
  THDEREAVIQQELVIHIGWIISNNPELFSGMLKIRIGWIIHAMEYELQIRGGDKPALDLYQLSPSEVKQL
  LLDILQPQQNGRCWLNRRQIDGSLNRTPTGFYDRVWQILERTPNGIIVAGKHLPQQPTLSDMTMYEMNFS
  LLVEDTLGNIDQPQYRQIVVELLMVVSIVLERNPELEFQDKVDLDRLVKEAFNEFQKDQSRLKEIEKQDD
  MTSFYNTPPLGKRGTCSYLTKAVMNLLLEGEVKPNNDDPCLIS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000293
• ORF Size: 3279 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000293.1, NP_000284.1
• RefSeq Size: 4284 bp
• RefSeq ORF: 3282 bp
• Locus ID: 5257
• Cytogenetics: 16q12.1
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Insulin signaling pathway
• Gene Summary: 'Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The beta subunit is the same in both the muscle and hepatic isoforms, encoded by this gene, which is a member of the phosphorylase b kinase regulatory subunit family. The gamma subunit also includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9B, also known as phosphorylase kinase deficiency of liver and muscle. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. Two pseudogenes have been found on chromosomes 14 and 20, respectively.[provided by RefSeq, Feb 2010]'