Phosphorylase B (PHKB) (NM_000293) Human Untagged Clone

CAT#: SC315172

PHKB (untagged)-Human phosphorylase kinase, beta (PHKB), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PHKB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene SC315172 ORF sequence for NM_000293, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGGGGCGGCGGGACTCACGGCAGAAGTGAGCTGGAAGGTCTTGGAGCGAAGAGCTCGGACCAAGC
  GCTCAGGCTCAGTTTATGAACCTCTTAAAAGCATTAATCTTCCAAGACCTGATAATGAAACTCTCTGGGA
  TAAGTTGGACCATTATTACAGAATTGTCAAGTCAACATTGCTGCTGTATCAAAGTCCAACTACCGGTCTC
  TTTCCCACTAAAACATGCGGTGGTGACCAGAAGGCCAAGATCCAGGACAGCCTATACTGCGCTGCTGGGG
  CCTGGGCTTTGGCTCTTGCATACAGGCGAATTGATGATGACAAGGGAAGGACCCATGAGCTGGAGCACTC
  AGCTATAAAATGCATGAGAGGAATTCTCTACTGCTATATGCGTCAGGCCGATAAGGTCCAGCAGTTTAAG
  CAGGATCCACGCCCAACAACATGTCTTCACTCTGTTTTCAATGTGCATACAGGAGATGAGTTGCTTTCCT
  ATGAGGAATATGGTCATCTTCAGATAAATGCAGTGTCACTTTATCTCCTTTACCTTGTGGAAATGATTTC
  CTCAGGACTCCAGATTATCTACAACACTGATGAGGTCTCTTTTATTCAAAACCTTGTATTTTGTGTGGAA
  AGAGTTTACCGTGTGCCTGACTTTGGTGTCTGGGAAAGAGGAAGCAAATATAATAATGGCAGCACAGAGC
  TACATTCGAGCTCGGTTGGTTTAGCAAAAGCAGCTCTAGAAGCAATTAATGGATTCAACCTTTTTGGCAA
  CCAGGGCTGTTCGTGGTCAGTTATATTTGTGGATCTCGATGCTCACAATCGCAACAGGCAAACTTTGTGC
  TCGCTGTTACCCAGAGAATCAAGATCACATAATACAGATGCTGCCCTGCTCCCCTGCATCAGTTATCCTG
  CATTTGCCCTGGATGATGAAGTTCTTTTTAGCCAGACACTTGATAAAGTGGTTAGAAAATTAAAAGGAAA
  ATATGGATTTAAACGTTTCTTGAGAGATGGGTATAGAACATCATTGGAAGATCCCAACAGATGCTACTAC
  AAGCCAGCTGAAATTAAGCTATTTGATGGCATTGAATGTGAATTTCCCATATTTTTCCTTTATATGATGA
  TTGATGGAGTTTTTAGAGGCAATCCTAAGCAAGTACAGGAATATCAGGATCTTTTGACTCCAGTACTTCA
  TCATACCACAGAAGGATATCCTGTTGTACCAAAGTACTATTATGTGCCAGCTGACTTTGTAGAATATGAA
  AAAAATAACCCTGGTAGTCAAAAACGATTTCCTAGCAACTGTGGCCGTGATGGAAAACTGTTTCTTTGGG
  GACAAGCACTTTATATCATCGCAAAACTCCTGGCTGATGAACTTATTAGTCCTAAAGACATTGATCCTGT
  CCAGCGCTATGTCCCACTAAAGGATCAACGTAACGTGAGCATGAGGTTTTCCAATCAGGGCCCACTGGAA
  AATGACTTGGTAGTTCATGTGGCACTTATAGCAGAAAGCCAAAGACTTCAAGTTTTTCTGAACACATATG
  GTATTCAAACTCAAACTCCTCAACAAGTAGAACCCATTCAGATATGGCCTCAGCAGGAGCTTGTGAAAGC
  TTATTTGCAGCTGGGTATCAATGAAAAGTTAGGACTCTCTGGAAGGCCAGACAGGCCCATTGGCTGCCTC
  GGGACATCAAAGATTTATCGCATTCTAGGAAAGACTGTGGTTTGTTACCCGATTATTTTCGACCTAAGTG
  ATTTCTACATGTCTCAGGATGTTTTCCTGCTGATAGATGACATAAAGAATGCGCTGCAGTTCATTAAACA
  ATATTGGAAAATGCATGGACGTCCACTTTTCCTTGTTCTCATCCGGGAAGACAATATAAGAGGTAGCCGG
  TTCAACCCCATATTAGATATGCTGGCAGCCCTTAAAAAAGGAATAATTGGAGGAGTCAAAGTTCATGTGG
  ATCGTCTACAGACACTAATATCTGGAGCTGTGGTAGAACAACTTGATTTCCTACGAATCAGTGACACAGA
  AGAGCTTCCAGAATTTAAGAGTTTTGAGGAACTAGAACCTCCCAAACATTCAAAAGTCAAACGGCAAAGC
  AGCACCCCTAGTGCTCCTGAACTGGGACAGCAGCCGGATGTCAACATTAGTGAATGGAAGGACAAACCCA
  CCCACGAAATTCTTCAAAAACTGAATGATTGCAGTTGTCTGGCTAGCCAAGCCATCCTGCTGGGTATACT
  GCTCAAAAGAGAAGGCCCCAACTTCATCACAAAGGAAGGTACCGTTTCTGATCACATTGAGAGAGTCTAT
  AGAAGAGCTGGCAGCCAAAAACTTTGGTTGGCGGTGCGCTACGGGGCTGCATTTACCCAGAAATTTTCTT
  CCTCTATAGCCCCACACATTACTACTTTTCTGGTACATGGGAAACAGGTAACTCTGGGTGCCTTTGGGCA
  TGAAGAAGAAGTTATCTCTAATCCTTTGTCTCCAAGAGTGATTCAAAACATCATCTATTATAAGTGTAAC
  ACCCATGATGAGAGGGAAGCGGTCATTCAGCAAGAACTGGTCATCCATATTGGCTGGATCATCTCCAATA
  ACCCTGAGTTATTCAGTGGCATGCTGAAAATACGAATCGGGTGGATCATCCATGCCATGGAGTATGAACT
  TCAGATCCGTGGCGGAGACAAGCCAGCCTTGGACTTGTATCAGCTGTCACCTAGTGAAGTTAAACAGCTT
  CTGCTGGATATTCTGCAGCCTCAACAGAATGGAAGATGTTGGCTGAACAGGCGTCAGATCGATGGGTCTT
  TGAATAGAACTCCCACCGGGTTCTATGACCGAGTGTGGCAGATTCTGGAGCGCACGCCCAATGGGATCAT
  TGTTGCTGGGAAGCATTTGCCTCAGCAACCAACCCTGTCAGATATGACCATGTATGAGATGAATTTCTCT
  CTCCTTGTTGAAGACACGTTGGGAAATATTGACCAGCCACAGTACAGACAGATCGTTGTAGAGTTACTTA
  TGGTTGTATCCATTGTACTGGAAAGAAACCCCGAGCTAGAATTTCAAGACAAAGTAGATCTAGACAGACT
  GGTCAAAGAAGCATTTAATGAATTTCAAAAAGATCAGAGTCGGCTAAAGGAAATTGAAAAACAAGATGAC
  ATGACTTCCTTTTACAACACTCCTCCCCTGGGAAAAAGAGGAACATGCAGCTATTTGACAAAGGCGGTGA
  TGAATCTGCTGCTGGAAGGAGAAGTCAAGCCAAACAATGATGACCCGTGTCTGATTAGCTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_000293
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000293.1, NP_000284.1
• RefSeq Size: 5491 bp
• RefSeq ORF: 3282 bp
• Locus ID: 5257
• Cytogenetics: 16q12.1
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Insulin signaling pathway
• Gene Summary: 'Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The beta subunit is the same in both the muscle and hepatic isoforms, encoded by this gene, which is a member of the phosphorylase b kinase regulatory subunit family. The gamma subunit also includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9B, also known as phosphorylase kinase deficiency of liver and muscle. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. Two pseudogenes have been found on chromosomes 14 and 20, respectively.[provided by RefSeq, Feb 2010]'
  Transcript Variant: This variant (1) encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.