MARK1 (NM_018650) Human Tagged ORF Clone

CAT#: RG221556

MARK1 (GFP-tagged) - Human MAP/microtubule affinity-regulating kinase 1 (MARK1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MARK1
• Synonyms: MARK; Par-1c; Par1c
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221556 representing NM_018650
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGCCCGGACGCCATTGCCGACGGTGAACGAGCGGGACACGGAAAATCATACATCTGTGGATGGAT
  ATACTGAACCACACATCCAGCCTACCAAGTCGAGTAGCAGACAGAACATCCCCCGGTGTAGAAACTCCAT
  TACGTCAGCAACAGATGAACAGCCTCACATTGGAAATTACCGTTTACAAAAAACAATAGGGAAGGGAAAT
  TTTGCCAAAGTCAAATTGGCAAGACACGTTCTAACTGGTAGAGAGGTTGCTGTGAAAATAATAGACAAAA
  CTCAGCTAAATCCTACCAGTCTACAAAAGTTATTTCGAGAAGTACGAATAATGAAGATACTGAATCATCC
  TAATATAGTAAAATTGTTTGAAGTTATTGAAACAGAGAAGACTCTCTATTTAGTCATGGAATACGCGAGT
  GGGGGTGAAGTATTTGATTACTTAGTTGCCCATGGAAGAATGAAAGAGAAAGAGGCCCGTGCAAAATTTA
  GGCAGATTGTATCTGCTGTACAGTATTGTCATCAAAAGTACATTGTTCACCGTGATCTTAAGGCTGAAAA
  CCTTCTCCTTGATGGTGATATGAATATTAAAATTGCTGACTTTGGTTTTAGTAATGAATTTACAGTTGGG
  AACAAATTGGACACATTTTGTGGAAGCCCACCCTATGCTGCTCCCGAGCTTTTCCAAGGAAAGAAGTATG
  ATGGGCCTGAAGTGGATGTGTGGAGTCTGGGCGTCATTCTCTATACATTAGTCAGTGGCTCCTTGCCTTT
  CGATGGCCAGAATTTAAAGGAACTGCGAGAGCGAGTTTTACGAGGGAAGTACCGTATTCCCTTCTATATG
  TCCACAGACTGTGAAAATCTTCTGAAGAAATTATTAGTCCTGAATCCAATAAAGAGAGGCAGCTTGGAAC
  AAATAATGAAAGATCGATGGATGAATGTTGGTCATGAAGAGGAAGAACTAAAGCCATATACTGAGCCTGA
  TCCGGATTTCAATGACACAAAAAGAATAGACATTATGGTCACCATGGGCTTTGCACGAGATGAAATAAAT
  GATGCCTTAATAAATCAGAAGTATGATGAAGTTATGGCTACTTATATTCTTCTAGGTAGAAAACCACCTG
  AATTTGAAGGTGGTGAATCGTTATCCAGTGGAAACTTGTGTCAGAGGTCCCGGCCCAGTAGTGACTTAAA
  CAACAGCACTCTTCAGTCCCCTGCTCACCTGAAGGTCCAGAGAAGTATCTCAGCAAATCAGAAGCAGCGG
  CGTTTCAGTGATCATGCTGGTCCATCCATTCCTCCTGCTGTATCATATACCAAAAGACCTCAGGCTAACA
  GTGTGGAAAGTGAACAGAAAGAGGAGTGGGACAAAGATGTGGCTCGAAAACTTGGCAGCACAACAGTTGG
  ATCAAAAAGCGAGATGACTGCAAGCCCTCTTGTAGGGCCAGAGAGGAAAAAATCTTCAACTATTCCAAGT
  AACAATGTGTATTCTGGAGGTAGCATGGCAAGAAGGAATACATATGTCTGTGAAAGGACCACAGATCGAT
  ACGTAGCATTGCAGAATGGAAAAGACAGCAGCCTTACGGAGATGTCTGTGAGTAGCATATCTTCTGCAGG
  CTCTTCTGTGGCCTCTGCTGTCCCCTCAGCACGACCCCGCCACCAGAAGTCCATGTCCACTTCTGGTCAT
  CCTATTAAAGTCACACTGCCAACCATTAAAGACGGCTCTGAAGCTTACCGGCCTGGTACAACCCAGAGAG
  TGCCTGCTGCTTCCCCATCTGCTCACAGTATTAGTACTGCGACTCCAGACCGGACCCGTTTTCCCCGAGG
  GAGCTCAAGCCGAAGCACTTTCCATGGTGAACAGCTCCGGGAGCGACGCAGCGTTGCTTATAATGGGCCA
  CCTGCTTCACCATCCCATGAAACGGGTGCATTTGCACATGCCAGAAGGGGAACGTCAACTGGTATAATAA
  GCAAAATCACATCCAAATTTGTTCGCAGGGATCCAAGTGAAGGCGAAGCCAGTGGCAGAACCGACACCTC
  AAGAAGTACATCAGGGGAACCAAAAGAAAGAGACAAGGAAGAGGGTAAAGATTCTAAGCCGCGTTCTTTG
  CGGTTCACATGGAGTATGAAGACCACTAGTTCAATGGACCCTAATGACATGATGAGAGAAATCCGAAAAG
  TGTTAGATGCAAATAACTGTGATTATGAGCAAAAAGAGAGATTTTTGCTTTTCTGTGTCCATGGAGACGC
  TAGACAGGATAGCCTCGTGCAGTGGGAGATGGAAGTCTGCAAGTTGCCACGACTGTCACTTAATGGGGTT
  CGCTTCAAGCGAATATCTGGGACATCTATTGCCTTTAAGAACATTGCATCAAAAATAGCAAATGAGCTTA
  AGCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221556 representing NM_018650
  Red=Cloning site Green=Tags(s)
  
  MSARTPLPTVNERDTENHTSVDGYTEPHIQPTKSSSRQNIPRCRNSITSATDEQPHIGNYRLQKTIGKGN
  FAKVKLARHVLTGREVAVKIIDKTQLNPTSLQKLFREVRIMKILNHPNIVKLFEVIETEKTLYLVMEYAS
  GGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKYIVHRDLKAENLLLDGDMNIKIADFGFSNEFTVG
  NKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYM
  STDCENLLKKLLVLNPIKRGSLEQIMKDRWMNVGHEEEELKPYTEPDPDFNDTKRIDIMVTMGFARDEIN
  DALINQKYDEVMATYILLGRKPPEFEGGESLSSGNLCQRSRPSSDLNNSTLQSPAHLKVQRSISANQKQR
  RFSDHAGPSIPPAVSYTKRPQANSVESEQKEEWDKDVARKLGSTTVGSKSEMTASPLVGPERKKSSTIPS
  NNVYSGGSMARRNTYVCERTTDRYVALQNGKDSSLTEMSVSSISSAGSSVASAVPSARPRHQKSMSTSGH
  PIKVTLPTIKDGSEAYRPGTTQRVPAASPSAHSISTATPDRTRFPRGSSSRSTFHGEQLRERRSVAYNGP
  PASPSHETGAFAHARRGTSTGIISKITSKFVRRDPSEGEASGRTDTSRSTSGEPKERDKEEGKDSKPRSL
  RFTWSMKTTSSMDPNDMMREIRKVLDANNCDYEQKERFLLFCVHGDARQDSLVQWEMEVCKLPRLSLNGV
  RFKRISGTSIAFKNIASKIANELKL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018650
• ORF Size: 2385 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018650.3, NP_061120.3
• RefSeq Size: 5293 bp
• RefSeq ORF: 2388 bp
• Locus ID: 4139
• Cytogenetics: 1q41
• Domains: UBA, pkinase, TyrKc, KA1, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: ''