MARK1 (NM_018650) Human Untagged Clone

CAT#: SC113408

MARK1 (untagged)-Human MAP/microtubule affinity-regulating kinase 1 (MARK1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MARK1
• Synonyms: MARK; Par-1c; Par1c
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_018650 edited
  ATGTCGGCCCGGACGCCATTGCCGACGGTGAACGAGCGGGACACGGAAAATCATACATCT
  GTGGATGGATATACTGAACCACACATCCAGCCTACCAAGTCGAGTAGCAGACAGAACATC
  CCCCGGTGTAGAAACTCCATTACGTCAGCAACAGATGAACAGCCTCACATTGGAAATTAC
  CGTTTACAAAAAACAATAGGGAAGGGAAATTTTGCCAAAGTCAAATTGGCAAGACACGTT
  CTAACTGGTAGAGAGGTTGCTGTGAAAATAATAGACAAAACTCAGCTAAATCCTACCAGT
  CTACAAAAGAAATATATCTACCTGTTTTCTTTAACAGTTATTTCGAGAAGTACGAATAAT
  GAAGATACTGAATCATCCTAATATAGTAAAATTGTTTGAAGTTATTGAAACAGAGAAGAC
  TCTCTATTTAGTCATGGAATACGCGAGTGGGGGTGAAGTATTTGATTACTTAGTTGCCCA
  TGGAAGAATGAAAGAGAAAGAGGCCCGTGCAAAATTTAGGCAGATTGTATCTGCTGTACA
  GTATTGTCATCAAAAGTACATTGTTCACCGTGATCTTAAGGCTGAAAACCTTCTCCTTGA
  TGGTGATATGAATATTAAAATTGCTGACTTTGGTTTTAGTAATGAATTTACAGTTGGGAA
  CAAATTGGACACATTTTGTGGAAGCCCACCCTATGCTGCTCCCGAGCTTTTCCAAGGAAA
  GAAGTATGATGGGCCTGAAGTGGATGTGTGGAGTCTGGGCGTCATTCTCTATACATTAGT
  CAGTGGCTCCTTGCCTTTCGATGGCCAGAATTTAAAGGAACTGCGAGAGCGAGTTTTACG
  AGGGAAGTACCGTATTCCCTTCTATATGTCCACAGACTGTGAAAATCTTCTGAAGAAATT
  ATTAGTCCTGAATCCAATAAAGAGAGGCAGCTTGGAACAAATAATGAAAGATCGATGGAT
  GAATGTTGGTCATGAAGAGGAAGAACTAAAGCCATATACTGAGCCTGATCCGGATTTCAA
  TGACACAAAAAGAATAGACATTATGGTCACCATGGGCTTTGCACGAGATGAAATAAATGA
  TGCCTTAATAAATCAGAAGTATGATGAAGTTATGGCTACTTATATTCTTCTAGGTAGAAA
  ACCACCTGAATTTGAAGGTGGTGAATCGTTATCCAGTGGAAACTTGTGTCAGAGGTCCCG
  GCCCAGTAGTGACTTAAACAACAGCACTCTTCAGTCCCCTGCTCACCTGAAGGTCCAGAG
  AAGTATCTCAGCAAATCAGAAGCAGCGGCGTTTCAGTGATCATGCTGGTCCATCCATTCC
  TCCTGCTGTATCATATACCAAAAGACCTCAGGCTAACAGTGTGGAAAGTGAACAGAAAGA
  GGAGTGGGACAAAGATGTGGCTCGAAAACTTGGCAGCACAACAGTTGGATCAAAAAGCGA
  GATGACTGCAAGCCCTCTTGTAGGGCCAGAGAGGAAAAAATCTTCAACTATTCCAAGTAA
  CAATGTGTATTCTGGAGGTAGCATGGCAAGAAGGAATACATATGTCTGTGAAAGGACCAC
  AGATCGATACGTAGCATTGCAGAATGGAAAAGACAGCAGCCTTACGGAGATGTCTGTGAG
  TAGCATATCTTCTGCAGGCTCTTCTGTGGCCTCTGCTGTCCCCTCAGCACGACCCCGCCA
  CCAGAAGTCCATGTCCACTTCTGGTCATCCTATTAAAGTCACACTGCCAACCATTAAAGA
  CGGCTCTGAAGCTTACCGGCCTGGTACAACCCAGAGAGTGCCTGCTGCTTCCCCATCTGC
  TCACAGTATTAGTACTGCGACTCCAGACCGGACCCGTTTTCCCCGAGGGAGCTCAAGCCG
  AAGCACTTTCCATGGTGAACAGCTCCGGGAGCGACGCAGCGTTGCTTATAATGGGCCACC
  TGCTTCACCATCCCATGAAACGGGTGCATTTGCACATGCCAGAAGGGGAACGTCAACTGG
  TATAATAAGCAAAATCACATCCAAATTTGTTCGCAGGGATCCAAGTGAAGGCGAAGCCAG
  TGGCAGAACCGACACCTCAAGAAGTACATCAGGGGAACCAAAAGAAAGAGACAAGGAAGA
  GGGTAAAGATTCTAAGCCGCGTTCTTTGCGGTTCACATGGAGTATGAAGACCACTAGTTC
  AATGGACCCTAATGACATGATGAGAGAAATCCGAAAAGTGTTAGATGCAAATAACTGTGA
  TTATGAGCAAAAAGAGAGATTTTTGCTTTTCTGTGTCCATGGAGACGCTAGACAGGATAG
  CCTCGTGCAGTGGGAGATGGAAGTCTGCAAGTTGCCACGACTGTCACTTAATGGGGTTCG
  CTTCAAGCGAATATCTGGGACATCTATTGCCTTTAAGAACATTGCATCAAAAATAGCAAA
  TGAGCTTAAGCTGTAAAGAAGTCCAAATTTACAGGTTCAGGGAAGATACATACATATATG
  AGGTACAGTTTTTGAATGTACTGGTAATGCCTAATGTGGTCTGCCTGTGAATCTCCCCAT
  GTAGAATTTGCCCTTAATGCAATAAGGTTATACATAGTTATGAACTGTAAAATTAAAGTC
  AGTATGAACTATAATAAATATCTGTAGCTTAAAAAGTAGGTTCACATGTACAGGTAAGTA
  TATTGTGTATTTCTGTTCATTTTCTGTTCATAGAGTTGTATAATAAAACATGATTGCTTA
  AAAACTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCC
  GCGGTCATAGCAGTTTCCTGAACAGATCCCGGGTGGCATCC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018650 unedited
  GGTTCAAATATTTGTATACGACTCACTATAGGGGGCGGCCGCGCAATTCGGCACGAGGCA
  GCGCCGGGCAACCGCCTCGCCCGAAGCCCTCCCTCGTTACTGTCCGCATACCCCGGCGGC
  GCCGCCGCGGGAAGCGGCTCCCCCTCCTCTTCCTCCGCGTCCTCTTCCCTCTTTCCCCCG
  CCGGGGCCGCTTGTTGCACCGCCCCGCGGCCTGCGGGAGCCGCTCGCCCCGGCCTTGTGC
  TCGCGTCCGCACCCCTTTCCTGTCGCCCCCCGGGGCCCGCACCACAGCCCGGCCGGCGAG
  ACCCCGGCCAGACCCCGCTGCCCGCACAAAATGTCGGCCCGGACGCCATTGCCGACGGTG
  AACGAGCGGGACACGGAAAATCATACATCTGTGGATGGATATACTGAACCACACATCCAG
  CCTACCAAGTCGCGTAGCAGACAGAACATCCCCCGGTGTAGAAACTCCATTACGTCAGCA
  ACAGATGAACAGCCTCACATTGGAAATTACCGTTTACAAAAAACAATAGGGAAGGGAAAT
  TTTGCCAAAGTCAAATTGGCAAGACACGTTCTAACTGGTAGAGAGGTTGCTGTGAAAATA
  ATAGACCAAACTCAGCTAAATCCTACCAGTCTACANAAGAAATATATCTACCTGTTTTCT
  TTAACAGTTATTTCGAGAAGTACGAATAATGAAGATACTGAATCATCCTAATATAGTAAA
  ATTTGTCTGAAGTTATTGAAACAGAGAAGACTCTCTATCTAGTCATGCAATACGCGAGTG
  GGGGTGAAGTATTTGATTACTTAGTTGCCCATGGAAGATTGAAAGAGAAAGAAGCCCGTG
  CAAAATTAGGCAGATTTATCTGCTGCCCAGATTGTCATCAAAATACATGGTCACCGTGAT
  CTTAGGCTGAAACCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_018650 unedited
  CGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACAAGT
  TTTTAAGCAATCATGTTTTATTATACAACTCTATGAACAGAAAATGAACAGAAATACACA
  ATATACTTACCTGTACATGTGAACCTACTTTTTAAGCTACAGATATTTATTATAGTTCAT
  ACTGACTTTAATTTTACAGTTCATAACTATGTATAACCTTATTGCATTAAGGGCAAATTC
  TACATGGGGAGATTCACAGGCAGACCACATTAGGCATTACCAGTACATTCAAAAACTGTA
  CCTCATATATGTATGTATCTTCCCTGAACCTGTAAATTTGGACTTCTTTACAGCTTAAGC
  TCATTTGCTATTTTTGATGCAATGTTCTTAAAGGCAATAGATGTCCCAGATATTCGCTTG
  AAGCGAACCCCATTAAGTGACAGTCGTGGCAACTTGCAGACTTCCATCTCCCACTGCACG
  AGGCTATCCTGTCTAGCGTCTCCATGGACACAGAAAAGCAAAAATCTCTCTTTTTGCTCA
  TAATCACAGTTATTTGCATCTAACACTTTTCGGATTTCTCTCATCATGTCATTAGGGGCC
  ATTGAACTAGTGGTCTTCATACTCCATGTGAAACCGCAAGAACGCGGCTTAAAATCTTTA
  CCCTCTTCCTTGGCTCTTTCTTTGGGNTCCCCTGATGTACTTCTTGGAGGGGTCGGNNTC
  TGCCACTGGCTTCGCCTTCACTTGGGATCCCTGCGAAAAATTGGGATGTGATTTTGCTTA
  TATACCAATTGACGGTTCCCTTCTTGGATGTGCAAATGCCACCCGTTCATGGGAGGGGAA
  ACAGGTGGCCCCTTATAGCCACGCTGGGTCGCTTCGGACCTGTTACATTGAAAGGGCTTC
  GTTTGACCCCTCGGGAAACGGGTCCGTCTGATTCCAGACTAAACGGGACAAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_018650
• Insert Size: 3180 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018650.2, NP_061120.2
• RefSeq Size: 4638 bp
• RefSeq ORF: 2277 bp
• Locus ID: 4139
• Cytogenetics: 1q41
• Domains: UBA, pkinase, TyrKc, KA1, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: ''
  Transcript Variant: This variant (2) uses an alternate in-frame splice site compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.