MARK1 (NM_018650) Human Untagged Clone

CAT#: SC323553

MARK1 (untagged)-Kinase deficient mutant (K89M) of Human MAP/microtubule affinity-regulating kinase 1 (MARK1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MARK1
• Synonyms: MARK; Par-1c; Par1c
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_018650, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCGGCCCGGACGCCATTGCCGACGGTGAACGAGCGGGACACGGAAAATCATACATCTGTGGATGGAT
  ATACTGAACCACACATCCAGCCTACCAAGTCGAGTAGCAGACAGAACATCCCCCGGTGTAGAAACTCCAT
  TACGTCAGCAACAGATGAACAGCCTCACATTGGAAATTACCGTTTACAAAAAACAATAGGGAAGGGAAAT
  TTTGCCAAAGTCAAATTGGCAAGACACGTTCTAACTGGTAGAGAGGTTGCTGTGAAAATAATAGACAAAA
  CTCAGCTAAATCCTACCAGTCTACAAAAGTTATTTCGAGAAGTACGAATAATGAAGATACTGAATCATCC
  TAATATAGTAAAATTGTTTGAAGTTATTGAAACAGAGAAGACTCTCTATTTAGTCATGGAATACGCGAGT
  GGGGGTGAAGTATTTGATTACTTAGTTGCCCATGGAAGAATGAAAGAGAAAGAGGCCCGTGCAAAATTTA
  GGCAGATTGTATCTGCTGTACAGTATTGTCATCAAAAGTACATTGTTCACCGTGATCTTAAGGCTGAAAA
  CCTTCTCCTTGATGGTGATATGAATATTAAAATTGCTGACTTTGGTTTTAGTAATGAATTTACAGTTGGG
  AACAAATTGGACACATTTTGTGGAAGCCCACCCTATGCTGCTCCCGAGCTTTTCCAAGGAAAGAAGTATG
  ATGGGCCTGAAGTGGATGTGTGGAGTCTGGGCGTCATTCTCTATACATTAGTCAGTGGCTCCTTGCCTTT
  CGATGGCCAGAATTTAAAGGAACTGCGAGAGCGAGTTTTACGAGGGAAGTACCGTATTCCCTTCTATATG
  TCCACAGACTGTGAAAATCTTCTGAAGAAATTATTAGTCCTGAATCCAATAAAGAGAGGCAGCTTGGAAC
  AAATAATGAAAGATCGATGGATGAATGTTGGTCATGAAGAGGAAGAACTAAAGCCATATACTGAGCCTGA
  TCCGGATTTCAATGACACAAAAAGAATAGACATTATGGTCACCATGGGCTTTGCACGAGATGAAATAAAT
  GATGCCTTAATAAATCAGAAGTATGATGAAGTTATGGCTACTTATATTCTTCTAGGTAGAAAACCACCTG
  AATTTGAAGGTGGTGAATCGTTATCCAGTGGAAACTTGTGTCAGAGGTCCCGGCCCAGTAGTGACTTAAA
  CAACAGCACTCTTCAGTCCCCTGCTCACCTGAAGGTCCAGAGAAGTATCTCAGCAAATCAGAAGCAGCGG
  CGTTTCAGTGATCATGCTGGTCCATCCATTCCTCCTGCTGTATCATATACCAAAAGACCTCAGGCTAACA
  GTGTGGAAAGTGAACAGAAAGAGGAGTGGGACAAAGATGTGGCTCGAAAACTTGGCAGCACAACAGTTGG
  ATCAAAAAGCGAGATGACTGCAAGCCCTCTTGTAGGGCCAGAGAGGAAAAAATCTTCAACTATTCCAAGT
  AACAATGTGTATTCTGGAGGTAGCATGGCAAGAAGGAATACATATGTCTGTGAAAGGACCACAGATCGAT
  ACGTAGCATTGCAGAATGGAAAAGACAGCAGCCTTACGGAGATGTCTGTGAGTAGCATATCTTCTGCAGG
  CTCTTCTGTGGCCTCTGCTGTCCCCTCAGCACGACCCCGCCACCAGAAGTCCATGTCCACTTCTGGTCAT
  CCTATTAAAGTCACACTGCCAACCATTAAAGACGGCTCTGAAGCTTACCGGCCTGGTACAACCCAGAGAG
  TGCCTGCTGCTTCCCCATCTGCTCACAGTATTAGTACTGCGACTCCAGACCGGACCCGTTTTCCCCGAGG
  GAGCTCAAGCCGAAGCACTTTCCATGGTGAACAGCTCCGGGAGCGACGCAGCGTTGCTTATAATGGGCCA
  CCTGCTTCACCATCCCATGAAACGGGTGCATTTGCACATGCCAGAAGGGGAACGTCAACTGGTATAATAA
  GCAAAATCACATCCAAATTTGTTCGCAGGGATCCAAGTGAAGGCGAAGCCAGTGGCAGAACCGACACCTC
  AAGAAGTACATCAGGGGAACCAAAAGAAAGAGACAAGGAAGAGGGTAAAGATTCTAAGCCGCGTTCTTTG
  CGGTTCACATGGAGTATGAAGACCACTAGTTCAATGGACCCTAATGACATGATGAGAGAAATCCGAAAAG
  TGTTAGATGCAAATAACTGTGATTATGAGCAAAAAGAGAGATTTTTGCTTTTCTGTGTCCATGGAGACGC
  TAGACAGGATAGCCTCGTGCAGTGGGAGATGGAAGTCTGCAAGTTGCCACGACTGTCACTTAATGGGGTT
  CGCTTCAAGCGAATATCTGGGACATCTATTGCCTTTAAGAACATTGCATCAAAAATAGCAAATGAGCTTA
  AGCTGTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_018650 unedited
  CCCGCCGTTGAGCAATGGGCGGTAGGCGATGTACGGTGGGAGGCTCTATATAAGCAGAGCTCGTTTAGTG
  AACCGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCAGCGCCGGGCA
  ACCGCCTCGCCCGAAGCCCTCCCTCGTTACTGTCCGCATACCCCGGCGGCGCCGCCGCGGGAAGCGGCTC
  CCCCTCCTCTTCCTCCGCGTCCTCTTCCCTCTTTCCCCCGCCGGGGCCGCTTGTTGCACCGCCCCGCGGC
  CTGCGGGAGCCGCTCGCCCCGGCCTTGTGCTCGCGTCCGCACCCCTTTCCTGTCGCCCCCCCGGGGCCCC
  GCACCACAGCCCGGCCGGCGAGACCCCCGGCCAGACCCCGCTGCCCGCACAAAATGTCGGCCCGGACGCC
  ATTGCCGACGGTGAACGAGCGGGACACGGAAATCATACATCTGTGGATGGATATACTGAACCACACATCC
  AGCCTACCAAGTCGAGTAGCAGACGACATCCCCCGGTGTAGAACTCATTACGTCAGCAACAGATGACAGC
  CTCCACATTGAATTACGGTTACCAAAAACAATAGGAGGAATTGGCCAAGTCAATTGGCAAGACCGTTCTA
  CTTGTAGAAGGTGCGCTGAGATGTATTATGACACAAACTTCGCTAATTCCCACCAGTGCTCTACAAGAGA
  AGTATCTCACCTGTGTTTCCTTACACGGTTAATTCCGAGATGTCGCAGATATTAGAGATACTCGGAACTC
  CTCTAATAGTGTAAATGTGTTGTAGATTTGGACACAGAGAACCCTCTAATACTCGGATATCCGGAATGTG
  GGGTGAGGATTTGATGACTCAATTGCCCTGAGAATGAGAGAAAGGCGCCGCTGCGCAATATGCGCATAGT
  TAGCCTGTCAGACAATGTAGTCCAAGTACTGTGACCCGATCTAGCTGAACCTCTCTCTATAGGGATGAAA
  TAATTTCGCGGCATGTGTTAAGAGTAACTG
  
  Kinase domain sequence

  >SC323553 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  AYAGAACATAGGGAGGGAATTTTGCCAAAGTCAAATTGGCAAGACACGTTCTAACTGGTAGAGAGGTTGC
  TGTGATGATAATAGACAAAACTCAGCTAAATCCTACCAGTCTACAAAAGAAATATATCTACCTGTTTTCT
  TTAACAGTTATTTCGAGAAGTACGAATAATGAAGATACTGAATCATCCTAATATAGTAAAATTGTTTGAA
  GTTATTGAAACAGAGAAGACTCTCTATTTAGTCATGGAATACGCG
  
• Restriction Sites: Please inquire
• ACCN: NM_018650
• Insert Size: 3180 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018650.2, NP_061120.2
• RefSeq Size: 4638 bp
• RefSeq ORF: 2277 bp
• Locus ID: 4139
• Cytogenetics: 1q41
• Domains: UBA, pkinase, TyrKc, KA1, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: ''
  Transcript Variant: This variant (2) uses an alternate in-frame splice site compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.