FBXO11 (NM_025133) Human Tagged ORF Clone

CAT#: RG222041

FBXO11 (GFP-tagged) - Human F-box protein 11 (FBXO11), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: FBXO11
• Synonyms: FBX11; IDDFBA; PRMT9; UBR6; UG063H01; VIT1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222041 representing NM_025133
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTGCAGAAGAATCAGGTCCTGGTGCACAAAATAGTCCATACCAACTTCGTAGAAAAACTCTTTTGC
  CGAAAAGAACAGCGTGTCCCACAAAGAACAGTATGGAGGGCGCCTCAACTTCAACTACAGAAAACTTTGG
  TCATCGTGCAAAACGTGCAAGAGTGTCTGGAAAATCACAAGATCTATCAGCAGCACCTGCTGAACAGTAT
  CTTCAGGAGAAACTGCCAGATGAAGTGGTTCTAAAAATCTTCTCTTACTTGCTGGAACAGGATCTTTGTA
  GAGCAGCTTGTGTATGTAAACGCTTCAGTGAACTTGCTAATGATCCAATTTTGTGGAAACGATTATATAT
  GGAAGTATTTGAATATACTCGCCCTATGATGCATCCTGAACCTGGAAAATTCTACCAGATTAATCCAGAA
  GAGTATGAACATCCAAATCCCTGGAAAGAGAGTTTCCAGCAGTTGTATAAAGGTGCACATGTAAAGCCAG
  GATTTGCTGAACATTTCTACAGTAACCCTGCAAGATATAAAGGAAGAGAAAATATGTTGTATTATGATAC
  TATTGAAGATGCCCTTGGTGGGGTACAAGAGGCTCATTTTGATGGACTTATCTTTGTTCATTCTGGAATA
  TATACTGATGAATGGATATATATTGAATCTCCAATCACCATGATTGGTGCAGCACCTGGGAAAGTGGCAG
  ACAAAGTTATAATTGAAAACACTAGAGATTCAACCTTCGTTTTTATGGAAGGCTCTGAAGATGCTTATGT
  TGGATATATGACAATAAGGTTTAACCCTGATGACAAATCTGCACAACACCACAATGCACACCACTGCTTA
  GAGATTACAGTAAATTGTAGCCCTATTATTGATCACTGTATCATCCGAAGTACATGTACAGTTGGTTCTG
  CAGTATGTGTTAGTGGTCAAGGAGCATGTCCCACCATCAAGCACTGTAACATCAGTGACTGTGAAAATGT
  TGGACTATATATAACAGATCATGCACAGGGAATATATGAGGATAATGAAATTTCCAATAATGCGTTAGCT
  GGGATTTGGGTTAAAAATCATGGAAACCCAATTATTAGACGGAATCATATTCATCATGGACGTGATGTTG
  GTGTGTTCACATTTGATCATGGCATGGGTTACTTTGAAAGTTGCAATATACACAGAAATAGGATAGCAGG
  CTTTGAAGTAAAAGCCTATGCTAACCCTACAGTGGTTCGATGTGAAATTCACCATGGGCAGACTGGAGGA
  ATATATGTCCATGAAAAAGGAAGAGGACAATTCATAGAGAATAAAATCTATGCAAACAACTTTGCAGGTG
  TATGGATTACCTCAAATAGTGACCCAACAATAAGGGGAAATTCTATATTTAATGGAAATCAAGGAGGAGT
  TTACATCTTTGGTGATGGACGAGGCCTTATTGAAGGAAATGACATTTATGGCAATGCATTAGCAGGAATT
  CAAATTAGGACAAACAGTTGTCCAATTGTTCGGCATAACAAAATTCATGATGGCCAGCATGGTGGGATTT
  ATGTGCATGAAAAGGGACAAGGAGTAATAGAAGAGAATGAAGTTTATAGTAACACTCTAGCTGGAGTCTG
  GGTGACAACTGGCAGCACTCCAGTACTGAGAAGAAACCGGATACACAGTGGCAAGCAGGTTGGTGTTTAT
  TTTTATGACAATGGACATGGAGTGCTAGAAGACAATGATATCTATAATCATATGTATTCAGGGGTTCAGA
  TAAGGACTGGAAGCAACCCCAAAATTAGACGCAACAAAATCTGGGGAGGACAGAATGGTGGAATTCTAGT
  TTATAATTCTGGTCTAGGCTGTATAGAAGACAATGAAATATTTGACAATGCAATGGCTGGAGTCTGGATT
  AAGACAGATAGTAATCCTACACTAAGAAGAAATAAAATCCATGATGGAAGAGATGGTGGCATCTGTATAT
  TTAATGGGGGTCGAGGTCTCCTTGAAGAAAATGATATTTTCAGGAATGCTCAAGCAGGTGTTCTCATCAG
  CACTAATAGTCATCCAATCTTAAGGAAAAACAGAATATTTGATGGATTTGCCGCAGGTATTGAAATTACA
  AATCACGCAACTGCAACACTAGAAGGCAATCAGATTTTTAACAACCGGTTTGGAGGCTTATTTTTAGCAT
  CTGGTGTTAATGTGACAATGAAAGATAACAAAATAATGAACAATCAAGATGCCATAGAAAAGGCTGTTAG
  TAGAGGCCAATGTTTATATAAAATATCAAGTTATACCAGCTATCCCATGCATGATTTCTACAGATGTCAT
  ACTTGTAACACCACAGATCGAAATGCCATATGTGTGAACTGCATTAAGAAGTGCCATCAGGGACATGATG
  TAGAGTTTATTAGACATGATAGGTTTTTCTGTGACTGTGGTGCTGGAACACTGTCTAATCCTTGTACATT
  AGCTGGTGAGCCTACACATGATACAGATACACTATATGACTCTGCTCCACCTATAGAATCTAATACATTG
  CAGCACAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222041 representing NM_025133
  Red=Cloning site Green=Tags(s)
  
  MVAEESGPGAQNSPYQLRRKTLLPKRTACPTKNSMEGASTSTTENFGHRAKRARVSGKSQDLSAAPAEQY
  LQEKLPDEVVLKIFSYLLEQDLCRAACVCKRFSELANDPILWKRLYMEVFEYTRPMMHPEPGKFYQINPE
  EYEHPNPWKESFQQLYKGAHVKPGFAEHFYSNPARYKGRENMLYYDTIEDALGGVQEAHFDGLIFVHSGI
  YTDEWIYIESPITMIGAAPGKVADKVIIENTRDSTFVFMEGSEDAYVGYMTIRFNPDDKSAQHHNAHHCL
  EITVNCSPIIDHCIIRSTCTVGSAVCVSGQGACPTIKHCNISDCENVGLYITDHAQGIYEDNEISNNALA
  GIWVKNHGNPIIRRNHIHHGRDVGVFTFDHGMGYFESCNIHRNRIAGFEVKAYANPTVVRCEIHHGQTGG
  IYVHEKGRGQFIENKIYANNFAGVWITSNSDPTIRGNSIFNGNQGGVYIFGDGRGLIEGNDIYGNALAGI
  QIRTNSCPIVRHNKIHDGQHGGIYVHEKGQGVIEENEVYSNTLAGVWVTTGSTPVLRRNRIHSGKQVGVY
  FYDNGHGVLEDNDIYNHMYSGVQIRTGSNPKIRRNKIWGGQNGGILVYNSGLGCIEDNEIFDNAMAGVWI
  KTDSNPTLRRNKIHDGRDGGICIFNGGRGLLEENDIFRNAQAGVLISTNSHPILRKNRIFDGFAAGIEIT
  NHATATLEGNQIFNNRFGGLFLASGVNVTMKDNKIMNNQDAIEKAVSRGQCLYKISSYTSYPMHDFYRCH
  TCNTTDRNAICVNCIKKCHQGHDVEFIRHDRFFCDCGAGTLSNPCTLAGEPTHDTDTLYDSAPPIESNTL
  QHN
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025133
• ORF Size: 2529 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_025133.3, NP_079409.3
• RefSeq Size: 3951
• RefSeq ORF: 2532
• Locus ID: 80204
• Domains: PbH1, CASH
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. It can function as an arginine methyltransferase that symmetrically dimethylates arginine residues, and it acts as an adaptor protein to mediate the neddylation of p53, which leads to the suppression of p53 function. This gene is known to be down-regulated in melanocytes from patients with vitiligo, a skin disorder that results in depigmentation. Polymorphisms in this gene are associated with chronic otitis media with effusion and recurrent otitis media (COME/ROM), a hearing loss disorder, and the knockout of the homologous mouse gene results in the deaf mouse mutant Jeff (Jf), a single gene model of otitis media. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jun 2010]