PLAGL1 (NM_006718) Human Tagged ORF Clone

CAT#: RG223260

PLAGL1 (GFP-tagged) - Human pleiomorphic adenoma gene-like 1 (PLAGL1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PLAGL1
• Synonyms: LOT1; ZAC; ZAC1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG223260 representing NM_006718
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACGTTCCCCTGCCAGTTATGTGGCAAGACGTTCCTCACCCTGGAGAAGTTCACGATTCACAATT
  ATTCCCACTCCAGGGAGCGGCCGTACAAGTGTGTGCAGCCTGACTGTGGCAAAGCCTTTGTTTCCAGATA
  TAAATTGATGAGGCATATGGCTACCCATTCTCCCCAGAAATCTCACCAGTGTGCTCACTGTGAGAAGACG
  TTCAACCGGAAAGACCACCTGAAAAACCACCTCCAGACCCACGACCCCAACAAAATGGCCTTTGGGTGTG
  AGGAGTGTGGGAAGAAGTACAACACCATGCTGGGCTATAAGAGGCACCTGGCCCTCCATGCGGCCAGCAG
  TGGGGACCTCACCTGTGGGGTCTGTGCCCTGGAGCTAGGGAGCACCGAGGTGCTACTGGACCACCTCAAA
  GCCCATGCGGAAGAGAAGCCCCCTAGCGGAACCAAGGAAAAGAAGCACCAGTGCGACCACTGTGAAAGAT
  GCTTCTACACCCGGAAGGATGTGCGACGCCACCTGGTGGTCCACACAGGATGCAAGGACTTCCTGTGCCA
  GTTCTGTGCCCAGAGATTTGGGCGCAAGGATCACCTCACCCGGCATACCAAGAAGACCCACTCACAGGAG
  CTGATGAAAGAGAGCTTGCAGACCGGAGACCTTCTGAGCACCTTCCACACCATCTCGCCTTCATTCCAAC
  TGAAGGCTGCTGCCTTGCCTCCTTTCCCTTTAGGAGCTTCTGCCCAGAACGGGCTTGCAAGTAGCTTGCC
  AGCTGAGGTCCATAGCCTCACCCTCAGTCCCCCAGAACAAGCCGCCCAGCCTATGCAGCCGCTGCCAGAG
  TCCCTGGCCTCCCTCCACCCCTCGGTATCCCCTGGCTCTCCTCCGCCACCCCTTCCCAATCACAAGTACA
  ACACCACTTCTACCTCATACTCCCCACTTGCAAGCCTGCCCCTCAAAGCAGATACTAAAGGTTTTTGCAA
  TATCAGTTTGTTTGAGGACTTGCCTCTGCAAGAGCCTCAGTCACCTCAAAAGCTCAACCCAGGTTTTGAT
  CTGGCTAAGGGAAATGCTGGTAAAGTAAACCTGCCCAAGGAGCTGCCTGCAGATGCTGTGAACCTAACAA
  TACCTGCCTCTCTGGACCTGTCCCCCCTGTTGGGCTTCTGGCAGCTGCCCCCTCCTGCTACCCAAAATAC
  CTTTGGGAATAGCACTCTTGCCCTGGGGCCTGGGGAATCTTTGCCCCACAGGTTAAGCTGTCTGGGGCAG
  CAGCAGCAAGAACCCCCACTTGCCATGGGCACTGTGAGCCTGGGCCAGCTCCCCCTGCCCCCCATCCCTC
  ATGTGTTCTCAGCTGGCACTGGCTCTGCCATCCTGCCTCATTTCCATCATGCATTCAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG223260 representing NM_006718
  Red=Cloning site Green=Tags(s)
  
  MATFPCQLCGKTFLTLEKFTIHNYSHSRERPYKCVQPDCGKAFVSRYKLMRHMATHSPQKSHQCAHCEKT
  FNRKDHLKNHLQTHDPNKMAFGCEECGKKYNTMLGYKRHLALHAASSGDLTCGVCALELGSTEVLLDHLK
  AHAEEKPPSGTKEKKHQCDHCERCFYTRKDVRRHLVVHTGCKDFLCQFCAQRFGRKDHLTRHTKKTHSQE
  LMKESLQTGDLLSTFHTISPSFQLKAAALPPFPLGASAQNGLASSLPAEVHSLTLSPPEQAAQPMQPLPE
  SLASLHPSVSPGSPPPPLPNHKYNTTSTSYSPLASLPLKADTKGFCNISLFEDLPLQEPQSPQKLNPGFD
  LAKGNAGKVNLPKELPADAVNLTIPASLDLSPLLGFWQLPPPATQNTFGNSTLALGPGESLPHRLSCLGQ
  QQQEPPLAMGTVSLGQLPLPPIPHVFSAGTGSAILPHFHHAFR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006718
• ORF Size: 1389 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006718.3, NP_006709.2
• RefSeq Size: 3411 bp
• RefSeq ORF: 1392 bp
• Locus ID: 5325
• Cytogenetics: 6q24.2
• Domains: zf-C2H2
• Protein Families: Transcription Factors
• Gene Summary: 'This gene encodes a C2H2 zinc finger protein that functions as a suppressor of cell growth. This gene is often deleted or methylated and silenced in cancer cells. In addition, overexpression of this gene during fetal development is thought to be the causal factor for transient neonatal diabetes mellitus (TNDM). Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding two different protein isoforms. The P1 downstream promoter of this gene is imprinted, with preferential expression from the paternal allele in many tissues. [provided by RefSeq, Nov 2015]'