PLAGL1 (NM_006718) Human Untagged Clone

CAT#: SC115928

PLAGL1 (untagged)-Human pleiomorphic adenoma gene-like 1 (PLAGL1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PLAGL1
• Synonyms: LOT1; ZAC; ZAC1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_006718, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCACGTTCCCCTGCCAGTTATGTGGCAAGACGTTCCTCACCCTGGAGAAGTTCACGATTCACAATT
  ATTCCCACTCCAGGGAGCGGCCGTACAAGTGTGTGCAGCCTGACTGTGGCAAAGCCTTTGTTTCCAGATA
  TAAATTGATGAGGCATATGGCTACCCATTCTCCCCAGAAATCTCACCAGTGTGCTCACTGTGAGAAGACG
  TTCAACCGGAAAGACCACCTGAAAAACCACCTCCAGACCCACGACCCCAACAAAATGGCCTTTGGGTGTG
  AGGAGTGTGGGAAGAAGTACAACACCATGCTGGGCTATAAGAGGCACCTGGCCCTCCATGCGGCCAGCAG
  TGGGGACCTCACCTGTGGGGTCTGTGCCCTGGAGCTAGGGAGCACCGAGGTGCTACTGGACCACCTCAAA
  GCCCATGCGGAAGAGAAGCCCCCTAGCGGAACCAAGGAAAAGAAGCACCAGTGCGACCACTGTGAAAGAT
  GCTTCTACACCCGGAAGGATGTGCGACGCCACCTGGTGGTCCACACAGGATGCAAGGACTTCCTGTGCCA
  GTTCTGTGCCCAGAGATTTGGGCGCAAGGATCACCTCACCCGGCATACCAAGAAGACCCACTCACAGGAG
  CTGATGAAAGAGAGCTTGCAGACCGGAGACCTTCTGAGCACCTTCCACACCATCTCGCCTTCATTCCAAC
  TGAAGGCTGCTGCCTTGCCTCCTTTCCCTTTAGGAGCTTCTGCCCAGAACGGGCTTGCAAGTAGCTTGCC
  AGCTGAGGTCCATAGCCTCACCCTCAGTCCCCCAGAACAAGCCGCCCAGCCTATGCAGCCGCTGCCAGAG
  TCCCTGGCCTCCCTCCACCCCTCGGTATCCCCTGGCTCTCCTCCGCCACCCCTTCCCAATCACAAGTACA
  ACACCACTTCTACCTCATACTCCCCACTTGCAAGCCTGCCCCTCAAAGCAGATACTAAAGGTTTTTGCAA
  TATCAGTTTGTTTGAGGACTTGCCTCTGCAAGAGCCTCAGTCACCTCAAAAGCTCAACCCAGGTTTTGAT
  CTGGCTAAGGGAAATGCTGGTAAAGTAAACCTGCCCAAGGAGCTGCCTGCAGATGCTGTGAACCTAACAA
  TACCTGCCTCTCTGGACCTGTCCCCCCTGTTGGGCTTCTGGCAGCTGCCCCCTCCTGCTACCCAAAATAC
  CTTTGGGAATAGCACTCTTGCCCTGGGGCCTGGGGAATCTTTGCCCCACAGGTTAAGCTGTCTGGGGCAG
  CAGCAGCAAGAACCCCCACTTGCCATGGGCACTGTGAGCCTGGGCCAGCTCCCCCTGCCCCCCATCCCTC
  ATGTGTTCTCAGCTGGCACTGGCTCTGCCATCCTGCCTCATTTCCATCATGCATTCAGATAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006718 unedited
  ATTCGGCACGAGGCAGATTACCTTTCTGCTCCTGAATCGCACCTGTGCCTCAGACTTTCT
  CCCCTCAGCTTGAGACTGCATGTAAACTGGGATGTGTGAAAGCAGGAAGCAAAGCTAGTG
  ACAGCTGAGAGGTCCATGTCTGGGTAGAACCAGGCCCACGATGCTGCCTCTCCCGTGGTC
  TGGAGTTCAGCTGCAGGGACTCTGCTGATGTGCCCAGCACCATCGTTCTGTTTGTGCTTA
  AATGGCACAGCATTTGGTCAGCACATCTGAAAAGGAAGGTGTGAGAAGCAAAGCCCATGG
  CCACGTTCCCCTGCCAGTTATGTGGCAAGACGTTCCTCACCCTGGAGAAGTTCACGATTC
  ACAATTATTCCCACTCCAGGGAGCGGCCGTACAAGTGTGTGCAGCCTGACTGTGGCAAAG
  CCTTTGTTTCCAGATATAAATTGATGAGGCATATGGCTACCCATTCTCCCCAGAAATCTC
  ACCAGTGTGCTCACTGTGAGAAGACGTTCAACCGGAAAGACCACCTGAAAAACCACCTCC
  AGACCCACGACCCCAACAAAATGGCCTTTGGGTGTGAGGAGTGTGGGAAGAAGTACAACA
  CCATGCTGGGCTATAAGAAGCACCTGGCCCTCCATGCGGNCAGCAGTGGGGACCTCACCT
  GTGGGGTCTGTGCCCTGGAGCTAGGGAGCACCGAAGTGCTACTGGACCACCTCAAGCCAT
  GCGGAAGAGAGCCCCCTAGCGGAACCAAGAAAGAAGCACCGTGCGACCACTGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006718 unedited
  CGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCTGTCAGATGTAACTGACA
  ACCAGTTTAGTCTTTCTTTAAGATAAATGAAGTGATAACTCACATTGCAGCATCTTGTGG
  AAAATATTCAAACTACATTTAAAATGCTTCTGTAAAATGGAGAACTCTATTGCTCCACAA
  GAAAATGTATATGTAAAACTACAGGTTGGCTATGGTAATAGTAACTAAACTACATCCAAC
  CCTGAAGGTAGAAAAATCCCTGAAAAGAAATACACAGCCTTACCATAGTATATCATATAA
  AGGCCATCTTCTTATTTTGAAAATTCTTCCAGATTTGGAGAAATCCAAACCTTTCCATAG
  TTCCCTTACTACGCACTGCCAATCCAAGTTATTCACTACCCATTTTGATCACGCGTTATT
  CATCATCCCCCTCACGCCTATACCTCATCACCTCTCTCCCCACCTCCCCCCCGCAGTTCC
  TCTATTTCTTTCCCCCCCCCCCCCCCACTTTTCCCCCCTCCCCTTTTTTTTCACACCTTA
  ACTATCTTTCCATCCCCTTTTTTCCCCCCATACTCCCAGTTTCCAATCCCCTTCCCCCTC
  CTCCCCTTTTACCCCCCATAATTCTTCCCCTCTCCGCCCTCTACTTCCATCCCGCCCCCC
  CCCTTTTTTTTTCCCCATTCCATCCCGCTCATTCTTCTTCCTCCCCTATTCCTTCTCCCT
  CTCCCCCCCCCAACCCCCCCCTTTATCCCACCACTTTTTATTAATCTCCATCATTCTCCT
  ACATATCCTCCTCCTTCCCACCTCCATTCACCCCTCTCCTTTTTCCCCCCCCTTTTTCCC
  CCTACTCGTCCCACTCTTTTCTCTCCATTTCCGTTACCTTCTCCCTTCATTTCCCCCCCC
  ACTTTTTTCCCCTTCCCTCTTTTTTCTCCATCATTCCCGTGCATCCCTTACCTATTCCTC
  CGTCCACCCCTCACACCATTTCCCCTTTCCACTCCCTTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006718
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006718.2, NP_006709.2
• RefSeq Size: 3411 bp
• RefSeq ORF: 3411 bp
• Locus ID: 5325
• Cytogenetics: 6q24.2
• Domains: zf-C2H2
• Protein Families: Transcription Factors
• Gene Summary: 'This gene encodes a C2H2 zinc finger protein that functions as a suppressor of cell growth. This gene is often deleted or methylated and silenced in cancer cells. In addition, overexpression of this gene during fetal development is thought to be the causal factor for transient neonatal diabetes mellitus (TNDM). Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding two different protein isoforms. The P1 downstream promoter of this gene is imprinted, with preferential expression from the paternal allele in many tissues. [provided by RefSeq, Nov 2015]'
  Transcript Variant: This variant (2) initiates from the P1 promoter and encodes the longer isoform (2). Variants 2, 3, 4, 5, 6, 10, 12, 15, 16, 17, 18, 19, 20, 22, 23, 25, 27, and 28 all encode the same isoform (2).