KIR2.3 (KCNJ4) (NM_152868) Human Tagged ORF Clone

CAT#: RG223261

KCNJ4 (GFP-tagged) - Human potassium inwardly-rectifying channel, subfamily J, member 4 (KCNJ4), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: KCNJ4
• Synonyms: HIR; HIRK2; HRK1; IRK-3; IRK3; Kir2.3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG223261 representing NM_152868
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACGGACACAGCCGCAACGGCCAGGCCCACGTGCCCCGGCGGAAGCGCCGCAACCGCTTCGTCAAGA
  AGAACGGCCAATGCAACGTGTACTTCGCCAACCTGAGCAACAAGTCGCAGCGCTACATGGCGGACATCTT
  CACCACCTGCGTGGACACGCGCTGGCGCTACATGCTCATGATCTTCTCCGCGGCCTTCCTTGTCTCCTGG
  CTCTTTTTCGGCCTCCTCTTCTGGTGTATCGCCTTCTTCCACGGTGACCTGGAGGCCAGCCCAGGGGTGC
  CTGCGGCGGGGGGCCCGGCGGCGGGTGGTGGCGGAGCAGCCCCGGTGGCCCCCAAGCCCTGCATCATGCA
  CGTGAACGGCTTCCTGGGTGCCTTCCTGTTCTCGGTGGAGACGCAGACGACCATCGGCTATGGGTTCCGG
  TGCGTGACAGAGGAGTGCCCGCTGGCAGTCATCGCTGTGGTGGTCCAGTCCATCGTGGGCTGCGTCATCG
  ACTCCTTCATGATTGGCACCATCATGGCCAAGATGGCGCGGCCCAAGAAGCGGGCGCAGACGTTGCTGTT
  CAGCCACCACGCGGTCATTTCGGTGCGCGACGGCAAGCTCTGCCTCATGTGGCGCGTGGGCAACCTGCGC
  AAGAGCCACATTGTGGAGGCCCACGTGCGGGCCCAGCTCATCAAGCCCTACATGACCCAGGAGGGCGAGT
  ACCTGCCCCTGGACCAGCGGGACCTCAACGTGGGCTATGACATCGGCCTGGACCGCATCTTCCTGGTGTC
  GCCCATCATCATTGTCCACGAGATCGACGAGGACAGCCCGCTTTATGGCATGGGCAAGGAGGAGCTGGAG
  TCGGAGGACTTTGAGATCGTGGTCATCCTGGAGGGCATGGTGGAGGCCACGGCCATGACCACCCAGGCCC
  GCAGCTCCTACCTGGCCAGCGAGATCCTGTGGGGCCACCGCTTTGAGCCTGTGGTCTTCGAGGAGAAGAG
  CCACTACAAGGTGGACTACTCACGTTTTCACAAGACCTACGAGGTGGCCGGCACGCCCTGCTGCTCGGCC
  CGGGAGCTGCAGGAGAGTAAGATCACCGTGCTGCCCGCCCCACCGCCCCCTCCCAGTGCCTTCTGCTACG
  AGAACGAGCTGGCCCTTATGAGCCAGGAGGAAGAGGAGATGGAGGAGGAGGCAGCTGCGGCGGCCGCGGT
  GGCCGCAGGCCTGGGCCTGGAGGCGGGTTCCAAGGAGGAGGCGGGCATCATCCGGATGCTGGAGTTCGGC
  AGCCACCTGGACCTGGAGCGCATGCAGGCTTCCCTCCCGCTGGACAACATCTCCTACCGCAGGGAGTCTG
  CCATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG223261 representing NM_152868
  Red=Cloning site Green=Tags(s)
  
  MHGHSRNGQAHVPRRKRRNRFVKKNGQCNVYFANLSNKSQRYMADIFTTCVDTRWRYMLMIFSAAFLVSW
  LFFGLLFWCIAFFHGDLEASPGVPAAGGPAAGGGGAAPVAPKPCIMHVNGFLGAFLFSVETQTTIGYGFR
  CVTEECPLAVIAVVVQSIVGCVIDSFMIGTIMAKMARPKKRAQTLLFSHHAVISVRDGKLCLMWRVGNLR
  KSHIVEAHVRAQLIKPYMTQEGEYLPLDQRDLNVGYDIGLDRIFLVSPIIIVHEIDEDSPLYGMGKEELE
  SEDFEIVVILEGMVEATAMTTQARSSYLASEILWGHRFEPVVFEEKSHYKVDYSRFHKTYEVAGTPCCSA
  RELQESKITVLPAPPPPPSAFCYENELALMSQEEEEMEEEAAAAAAVAAGLGLEAGSKEEAGIIRMLEFG
  SHLDLERMQASLPLDNISYRRESAI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152868
• ORF Size: 1335 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152868.1, NP_690607.1
• RefSeq Size: 2063 bp
• RefSeq ORF: 1338 bp
• Locus ID: 3761
• Cytogenetics: 22q13.1
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Gene Summary: 'Several different potassium channels are known to be involved with electrical signaling in the nervous system. One class is activated by depolarization whereas a second class is not. The latter are referred to as inwardly rectifying K+ channels, and they have a greater tendency to allow potassium to flow into the cell rather than out of it. This asymmetry in potassium ion conductance plays a key role in the excitability of muscle cells and neurons. The protein encoded by this gene is an integral membrane protein and member of the inward rectifier potassium channel family. The encoded protein has a small unitary conductance compared to other members of this protein family. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]'