Bcl2 Binding component 3 (BBC3) (NM_001127242) Human Tagged ORF Clone

CAT#: RG225019

BBC3 (GFP-tagged) - Human BCL2 binding component 3 (BBC3), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: BBC3
• Synonyms: JFY-1; JFY1; PUMA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG225019 representing NM_001127242
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAATTTGGCATGGGGTCTGCCCAGGCATGTCCATGCCAGGTGCCCAGGGCTGCTTCCACGACGTGGG
  TCCCCTGCCAGATTTGTGAGACAAGAGGAGCAGCAGCGGCACCGCCCCTCACCCTGGAGGGTCCTGTACA
  ATCTCATCATGGGACTCCTGCCCTTACCCAGGGGCCACAGAGCCCCCGAGATGGAGCCCAATTAGGTGCC
  TGCACCCGCCCGGTGGACGTCAGGGACTCGGGGGGCAGGCCCCTCCCACCTCCTGACACCCTGGCCAGCG
  CGGGGGACTTTCTCTGCACCATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG225019 representing NM_001127242
  Red=Cloning site Green=Tags(s)
  
  MKFGMGSAQACPCQVPRAASTTWVPCQICETRGAAAAPPLTLEGPVQSHHGTPALTQGPQSPRDGAQLGA
  CTRPVDVRDSGGRPLPPPDTLASAGDFLCTM
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001127242
• ORF Size: 303 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001127242.2, NP_001120714.1
• RefSeq Size: 1359
• RefSeq ORF: 306
• Locus ID: 27113
• Protein Families: Druggable Genome
• Protein Pathways: Huntington's disease, p53 signaling pathway
• Gene Summary: This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]