GSDME (NM_001127454) Human Tagged ORF Clone

CAT#: RG225486

DFNA5 (GFP-tagged) - Human deafness, autosomal dominant 5 (DFNA5), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GSDME
• Synonyms: DFNA5; ICERE-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG225486 representing NM_001127454
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTGCCAAAGCAACCAGGAATTTTCTTAGAGAAGTTGATGCTGATGGTGACCTGATTGCAGTATCAA
  ATCTGAATGACTCTGATAAGTTACAGCTTCTAAGTCTGGTGACAAAAAAGAAGAGATTCTGGTGCTGGCA
  GAGACCCAAGTACCAGTTTTTATCCCTCACCCTTGGCGATGTACTCATAGAAGACCAATTTCCGAGTCCA
  GTGGTCGTGGAGTCGGACTTTGTGAAATACGAGGGCAAGTTTGCAAACCACGTGAGTGGAACCCTGGAGA
  CTGCACTGGGGAAGGTCAAGCTGAACCTGGGGGGCAGCAGCCGCGTAGAGAGCCAGTCTTCATTTGGAAC
  CCTGAGGAAGCAGGAGGTGGATTTGCAGCAGCTCATCAGAGACTCTGCCGAGAGAACAATAAATCTGAGA
  AACCCTGTGCTCCAGCAGGTGCTGGAGGGAAGGAATGAGGTCCTGTGCGTTTTGACACAGAAGATCACGA
  CGATGCAGAAGTGTGTGATCTCTGAGCACATGCAGGTCGAGGAGAAGTGTGGTGGCATCGTGGGCATCCA
  GACCAAGACGGTGCAGGTGTCAGCGACGGAGGATGGGAATGTCACCAAGGACTCCAACGTGGTGCTGGAG
  ATCCCAGCTGCCACCACCATTGCCTACGGTGTCATTGAGTTATACGTGAAACTGGACGGCCTGTTCGAGT
  TCTGCCTTCTCCGAGGGAAGCAAGGTGGCTTCGAGAACAAGAAGAGAATTGACTCTGTCTACCTGGACCA
  CCTGGTCTTTCGAGAGTTTGCATTCATAGACATGCCAGATGCTGCGCATGGGATATCTTCCCAGGATGGA
  CCATTAAGTGTTTTAAAGCAAGCGACCCTGCTCCTGGAGAGGAATTTCCATCCATTTGCGGAGCTGCCTG
  AGCCACAACAGACAGCTTTGAGTGACATCTTCCAGGCGGTCGTATTTGATGATGAACTACTCATGGTCCT
  GGAACCAGTGTGCGATGACCTGGTCAGCGGCCTCTCGCCCACAGTGGCGGTGCTGGGGGAGCTGAAGCCC
  CGGCAGCAGCAGGACCTTGTGGCCTTCCTGCAGCTGGTGGGGTGCAGCTTACAGGGTGGGTGTCCGGGCC
  CCGAGGATGCAGGCAGCAAGCAGCTGTTTATGACAGCCTACTTCTTGGTCAGTGCCCTCGCAGAAATGCC
  AGATAGCGCAGCAGCTCTGCTGGGCACTTGCTGCAAACTCCAGATCATTCCCACACTGTGCCACTTGCTT
  CGTGCTCTGTCTGATGATGGAGTATCTGATCTTGAAGACCCAACCTTGACTCCCCTGAAAGATACAGAAA
  GGTTTGGGATTGTGCAGCGCTTGTTTGCCTCAGCTGACATTAGTCTGGAGAGACTGAAGTCATCTGTGAA
  AGCTGTCATTCTGAAGGACTCTAAAGTCTTCCCACTGCTTCTTTGTATAACCCTGAATGGACTCTGTGCT
  TTAGGCAGAGAACATTCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG225486 representing NM_001127454
  Red=Cloning site Green=Tags(s)
  
  MFAKATRNFLREVDADGDLIAVSNLNDSDKLQLLSLVTKKKRFWCWQRPKYQFLSLTLGDVLIEDQFPSP
  VVVESDFVKYEGKFANHVSGTLETALGKVKLNLGGSSRVESQSSFGTLRKQEVDLQQLIRDSAERTINLR
  NPVLQQVLEGRNEVLCVLTQKITTMQKCVISEHMQVEEKCGGIVGIQTKTVQVSATEDGNVTKDSNVVLE
  IPAATTIAYGVIELYVKLDGLFEFCLLRGKQGGFENKKRIDSVYLDHLVFREFAFIDMPDAAHGISSQDG
  PLSVLKQATLLLERNFHPFAELPEPQQTALSDIFQAVVFDDELLMVLEPVCDDLVSGLSPTVAVLGELKP
  RQQQDLVAFLQLVGCSLQGGCPGPEDAGSKQLFMTAYFLVSALAEMPDSAAALLGTCCKLQIIPTLCHLL
  RALSDDGVSDLEDPTLTPLKDTERFGIVQRLFASADISLERLKSSVKAVILKDSKVFPLLLCITLNGLCA
  LGREHS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001127454
• ORF Size: 1491 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001127454.1, NP_001120926.1
• RefSeq Size: 2291 bp
• RefSeq ORF: 999 bp
• Locus ID: 1687
• Cytogenetics: 7p15.3
• Protein Families: Druggable Genome
• Gene Summary: 'Hearing impairment is a heterogeneous condition with over 40 loci described. The protein encoded by this gene is expressed in fetal cochlea, however, its function is not known. Nonsyndromic hearing impairment is associated with a mutation in this gene. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'