GSDME (NM_001127454) Human Untagged Clone

CAT#: SC322982

DFNA5 (untagged)-Human deafness, autosomal dominant 5 (DFNA5), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GSDME
• Synonyms: DFNA5; ICERE-1
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001127454, the custom clone sequence may differ by one or more nucleotides
  ATGCAGAAGTGTGTGATCTCTGAGCACATGCAGGTCGAGGAGAAGTGTGGTGGCATCGTG
  GGCATCCAGACCAAGACGGTGCAGGTGTCAGCGACGGAGGATGGGAATGTCACCAAGGAC
  TCCAACGTGGTGCTGGAGATCCCAGCTGCCACCACCATTGCCTACGGTGTCATTGAGTTA
  TACGTGAAACTGGACGGCCAGTTCGAGTTCTGCCTTCTCCGAGGGAAGCAAGGTGGCTTC
  GAGAACAAGAAGAGAATTGACTCTGTCTACCTGGACCCCCTGGTCTTTCGAGAGTTTGCA
  TTCATAGACATGCCAGATGCTGCGCATGGGATATCTTCCCAGGATGGACCATTAAGTGTT
  TTAAAGCAAGCGACCCTGCTCCTGGAGAGGAATTTCCATCCATTTGCGGAGCTGCCTGAG
  CCACAACAGACAGCTTTGAGTGACATCTTCCAGGCGGTCCTATTTGATGATGAACTACTC
  ATGGTCCTGGAACCAGTGTGCGATGACCTGGTCAGCGGCCTCTCGCCCACAGTGGCGGTG
  CTGGGGGAGCTGAAGCCCCGGCAGCAGCAGGACCTTGTGGCCTTCCTGCAGCTGGTGGGG
  TGCAGCTTACAGGGTGGGTGTCCGGGCCCCGAGGATGCAGGCAGCAAGCAGCTGTTTATG
  ACAGCCTACTTCTTGGTCAGTGCCCTCGCAGAAATGCCAGATAGCGCAGCAGCTCTGCTG
  GGCACTTGCTGCAAACTCCAGATCATTCCCACACTGTGCCACTTGCTTCGTGCTCTGTCT
  GATGATGGAGTATCTGATCTTGAAGACCCAACCTTGACTCCCCTGAAAGATACAGAAAGG
  TTTGGGATTGTGCAGCGCTTGTTTGCCTCAGCTGACATTAGTCTGGAGAGACTGAAGTCA
  TCTGTGAAAGCTGTCATTCTGAAGGACTCTAAAGTCTTCCCACTGCTTCTTTGTATAACC
  CTGAATGGACTCTGTGCTTTAGGCAGAGAACATTCA
  
• Restriction Sites: Please inquire
• ACCN: NM_001127454
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001127454.1, NP_001120926.1
• RefSeq Size: 2291 bp
• RefSeq ORF: 999 bp
• Locus ID: 1687
• Cytogenetics: 7p15.3
• Protein Families: Druggable Genome
• Gene Summary: 'Hearing impairment is a heterogeneous condition with over 40 loci described. The protein encoded by this gene is expressed in fetal cochlea, however, its function is not known. Nonsyndromic hearing impairment is associated with a mutation in this gene. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (3) lacks an alternate exon compared to variant 1, resulting in an isoform (b) that uses a downstream start site compared to isoform a.