Lubricin (PRG4) (NM_001127709) Human Tagged ORF Clone
CAT#: RG226380
- TrueORF®
PRG4 (GFP-tagged) - Human proteoglycan 4 (PRG4), transcript variant C
"NM_001127709" in other vectors (2)
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Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | PRG4 |
Synonyms | CACP; HAPO; JCAP; MSF; SZP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG226380 representing NM_001127709
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCATGGAAAACACTTCCCATTTACCTGTTGTTGCTGCTGTCTGTTTTCGTGATTCAGCAAGTTTCAT CTCAAGATTTATCAAGCTGTGCAGGGAGATGTGGGGAAGGGTATTCTAGAGATGCCACCTGCAACTGTGA TTATAACTGTCAACACTACATGGAGTGCTGCCCTGATTTCAAGAGAGTCTGCACTGCGGAGCTTTCCTGT AAAGGCCGCTGCTTTGAGTCCTTCGAGAGAGGGAGGGAGTGTGACTGCGACGCCCAATGTAAGAAGTATG ACAAGTGCTGTCCCGATTATGAGAGTTTCTGTGCAGAAGTAAAAGATAACAAGAAGAACAGAACTAAAAA GAAACCTACCCCCAAACCACCAGTTGTAGATGAAGCTGGAAGTGGATTGGACAATGGTGACTTCAAGGTC ACAACTCCTGACACGTCTACCACCCAACACAATAAAGTCAGCACATCTCCCAAGATCACAACAGCAAAAC CAATAAATCCCAGACCCAGTCTTCCACCTAATTCTGATACATCTAAAGAGACGTCTTTGACAGTGAATAA AGAGACAACAGTTGAAACTAAAGAAACTACTACAACAAATAAACAGACTTCAACTGATGGAAAAGAGAAG ACTACTTCCGCTAAAGAGACACAAAGTATAGAGAAAACATCTGCTAAAGATTTAGCACCCACATCTAAAG TGCTGGCTAAACCTACACCCAAAGCTGAAACTACAACCAAAGGCCCTGCTCTCACCACTCCCAAGGAGCC CACGCCCACCACTCCCAAGGAGCCTGCATCTACCACACCCAAAGAGCCCACACCTACCACCATCAAGTCT GCACCCACCACCCCCAAGGAGCCTGCACCCACCACCACCAAGTCTGCACCCACCACTCCCAAGGAGCCTG CACCCACCACCACCAAGGAGCCTGCACCCACCACTCCCAAGGAGCCTGCACCCACCACCACCAAGGAGCC TGCACCCACCACCACCAAGTCTGCACCCACCACTCCCAAGGAGCCTGCACCCACCACCCCCAAGAAGCCT GCCCCAACTACCCCCAAGGAGCCTGCACCCACCACTCCCAAGGAGCCTACACCCACCACTCCCAAGGAGC CTGCACCCACCACCAAGGAGCCTGCACCCACCACTCCCAAAGAGCCTGCACCCACTGCCCCCAAGAAGCC TGCCCCAACTACCCCCAAGGAGCCTGCACCCACCACTCCCAAGGAGCCTGCACCCACCACCACCAAGGAG CCTTCACCCACCACTCCCAAGGAGCCTGCACCCACCACCACCAAGTCTGCACCCACCACTACCAAGGAGC CTGCACCCACCACTACCAAGTCTGCACCCACCACTCCCAAGGAGCCTTCACCCACCACCACCAAGGAGCC TGCACCCACCACTCCCAAGGAGCCTGCACCCACCACCCCCAAGAAGCCTGCCCCAACTACCCCCAAGGAG CCTGCACCCACCACTCCCAAGGAACCTGCACCCACCACCACCAAGAAGCCTGCACCCACCACTCCCAAAG AGCCTGCCCCAACTACCCCCAAGGAGACTGCACCCACCACCCCCAAGAAGCTCACGCCCACCACCCCCGA GAAGCTCGCACCCACCACCCCTGAGAAGCCCGCACCCACCACCCCTGAGGAGCTCGCACCCACCACCCCT GAGGAGCCCACACCCACCACCCCTGAGGAGCCTGCTCCCACCACTCCCAAGGCAGCGGCTCCCAACACCC CTAAGGAGCCTGCTCCAACTACCCCTAAGGAGCCTGCTCCAACTACCCCTAAGGAGCCTGCTCCAACTAC CCCTAAGGAGACTGCTCCAACTACCCCTAAAGGGACTGCTCCAACTACCCTCAAGGAACCTGCACCCACT ACTCCCAAGAAGCCTGCCCCCAAGGAGCTTGCACCCACCACCACCAAGGAGCCCACATCCACCACCTGTG ACAAGCCCGCTCCAACTACCCCTAAGGGGACTGCTCCAACTACCCCTAAGGAGCCTGCTCCAACTACCCC TAAGGAGCCTGCTCCAACTACCCCTAAGGGGACTGCTCCAACTACCCTCAAGGAACCTGCACCCACTACT CCCAAGAAGCCTGCCCCCAAGGAGCTTGCACCCACCACCACCAAGGGGCCCACATCCACCACCTCTGACA AGCCTGCTCCAACTACACCTAAGGAGACTGCTCCAACTACCCCCAAGGAGCCTGCACCCACTACCCCCAA GAAGCCTGCTCCAACTACTCCTGAGACACCTCCTCCAACCACTTCAGAGGTCTCTACTCCAACTACCACC AAGGAGCCTACCACTATCCACAAAAGCCCTGATGAATCAACTCCTGAGCTTTCTGCAGAACCCACACCAA AAGCTCTTGAAAACAGTCCCAAGGAACCTGGTGTACCTACAACTAAGACTCCTGCAGCGACTAAACCTGA AATGACTACAACAGCTAAAGACAAGACAACAGAAAGAGACTTACGTACTACACCTGAAACTACAACTGCT GCACCTAAGATGACAAAAGAGACAGCAACTACAACAGAAAAAACTACCGAATCCAAAATAACAGCTACAA CCACACAAGTAACATCTACCACAACTCAAGATACCACACCATTCAAAATTACTACTCTTAAAACAACTAC TCTTGCACCCAAAGTAACTACAACAAAAAAGACAATTACTACCACTGAGATTATGAACAAACCTGAAGAA ACAGCTAAACCAAAAGACAGAGCTACTAATTCTAAAGCGACAACTCCTAAACCTCAAAAGCCAACCAAAG CACCCAAAAAACCCACTTCTACCAAAAAGCCAAAAACAATGCCTAGAGTGAGAAAACCAAAGACGACACC AACTCCCCGCAAGATGACATCAACAATGCCAGAATTGAACCCTACCTCAAGAATAGCAGAAGCCATGCTC CAAACCACCACCAGACCTAACCAAACTCCAAACTCCAAACTAGTTGAAGTAAATCCAAAGAGTGAAGATG CAGGTGGTGCTGAAGGAGAAACACCTCATATGCTTCTCAGGCCCCATGTGTTCATGCCTGAAGTTACTCC CGACATGGATTACTTACCGAGAGTACCCAATCAAGGCATTATCATCAATCCCATGCTTTCCGATGAGACC AATATATGCAATGGTAAGCCAGTAGATGGACTGACTACTTTGCGCAATGGGACATTAGTTGCATTCCGAG GTCATTATTTCTGGATGCTAAGTCCATTCAGTCCACCATCTCCAGCTCGCAGAATTACTGAAGTTTGGGG TATTCCTTCCCCCATTGATACTGTTTTTACTAGGTGCAACTGTGAAGGAAAAACTTTCTTCTTTAAGGAT TCTCAGTACTGGCGTTTTACCAATGATATAAAAGATGCAGGGTACCCCAAACCAATTTTCAAAGGATTTG GAGGACTAACTGGACAAATAGTGGCAGCGCTTTCAACAGCTAAATATAAGAACTGGCCTGAATCTGTGTA TTTTTTCAAGAGAGGTGGCAGCATTCAGCAGTATATTTATAAACAGGAACCTGTACAGAAGTGCCCTGGA AGAAGGCCTGCTCTAAATTATCCAGTGTATGGAGAAACGACACAGGTTAGGAGACGTCGCTTTGAACGTG CTATAGGACCTTCTCAAACACACACCATCAGAATTCAATATTCACCTGCCAGACTGGCTTATCAAGACAA AGGTGTCCTTCATAATGAAGTTAAAGTGAGTATACTGTGGAGAGGACTTCCAAATGTGGTTACCTCAGCT ATATCACTGCCCAACATCAGAAAACCTGACGGCTATGATTACTATGCCTTTTCTAAAGATCAATACTATA ACATTGATGTGCCTAGTAGAACAGCAAGAGCAATTACTACTCGTTCTGGGCAGACCTTATCCAAAGTCTG GTACAACTGTCCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG226380 representing NM_001127709
Red=Cloning site Green=Tags(s) MAWKTLPIYLLLLLSVFVIQQVSSQDLSSCAGRCGEGYSRDATCNCDYNCQHYMECCPDFKRVCTAELSC KGRCFESFERGRECDCDAQCKKYDKCCPDYESFCAEVKDNKKNRTKKKPTPKPPVVDEAGSGLDNGDFKV TTPDTSTTQHNKVSTSPKITTAKPINPRPSLPPNSDTSKETSLTVNKETTVETKETTTTNKQTSTDGKEK TTSAKETQSIEKTSAKDLAPTSKVLAKPTPKAETTTKGPALTTPKEPTPTTPKEPASTTPKEPTPTTIKS APTTPKEPAPTTTKSAPTTPKEPAPTTTKEPAPTTPKEPAPTTTKEPAPTTTKSAPTTPKEPAPTTPKKP APTTPKEPAPTTPKEPTPTTPKEPAPTTKEPAPTTPKEPAPTAPKKPAPTTPKEPAPTTPKEPAPTTTKE PSPTTPKEPAPTTTKSAPTTTKEPAPTTTKSAPTTPKEPSPTTTKEPAPTTPKEPAPTTPKKPAPTTPKE PAPTTPKEPAPTTTKKPAPTTPKEPAPTTPKETAPTTPKKLTPTTPEKLAPTTPEKPAPTTPEELAPTTP EEPTPTTPEEPAPTTPKAAAPNTPKEPAPTTPKEPAPTTPKEPAPTTPKETAPTTPKGTAPTTLKEPAPT TPKKPAPKELAPTTTKEPTSTTCDKPAPTTPKGTAPTTPKEPAPTTPKEPAPTTPKGTAPTTLKEPAPTT PKKPAPKELAPTTTKGPTSTTSDKPAPTTPKETAPTTPKEPAPTTPKKPAPTTPETPPPTTSEVSTPTTT KEPTTIHKSPDESTPELSAEPTPKALENSPKEPGVPTTKTPAATKPEMTTTAKDKTTERDLRTTPETTTA APKMTKETATTTEKTTESKITATTTQVTSTTTQDTTPFKITTLKTTTLAPKVTTTKKTITTTEIMNKPEE TAKPKDRATNSKATTPKPQKPTKAPKKPTSTKKPKTMPRVRKPKTTPTPRKMTSTMPELNPTSRIAEAML QTTTRPNQTPNSKLVEVNPKSEDAGGAEGETPHMLLRPHVFMPEVTPDMDYLPRVPNQGIIINPMLSDET NICNGKPVDGLTTLRNGTLVAFRGHYFWMLSPFSPPSPARRITEVWGIPSPIDTVFTRCNCEGKTFFFKD SQYWRFTNDIKDAGYPKPIFKGFGGLTGQIVAALSTAKYKNWPESVYFFKRGGSIQQYIYKQEPVQKCPG RRPALNYPVYGETTQVRRRRFERAIGPSQTHTIRIQYSPARLAYQDKGVLHNEVKVSILWRGLPNVVTSA ISLPNIRKPDGYDYYAFSKDQYYNIDVPSRTARAITTRSGQTLSKVWYNCP TRTRRLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001127709 |
ORF Size | 3933 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Reference Data | |
RefSeq | NM_001127709.1, NP_001121181.1 |
RefSeq Size | 4780 |
RefSeq ORF | 3936 |
Locus ID | 10216 |
Gene Summary | The protein encoded by this gene is a large proteoglycan that is synthesized by chondrocytes located at the surface of articular cartilage and by some synovial lining cells. This protein contains both chondroitin sulfate and keratan sulfate glycosaminoglycans. It functions as a boundary lubricant at the cartilage surface and contributes to the elastic absorption and energy dissipation of synovial fluid. Mutations in this gene result in camptodactyly-arthropathy-coxa vara-pericarditis syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] |
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