NDE1 (NM_001143979) Human Tagged ORF Clone

CAT#: RG227919

NDE1 (GFP-tagged) - Human nudE nuclear distribution gene E homolog 1 (A. nidulans) (NDE1), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NDE1
• Synonyms: HOM-TES-87; LIS4; MHAC; NDE; NUDE; NUDE1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG227919 representing NM_001143979
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGACTCCGGAAAGACTTTCAGCTCCGAGGAGGAAGAAGCTAACTATTGGAAAGATCTGGCGATGA
  CCTACAAACAGAGGGCAGAAAATACGCAAGAGGAACTCCGAGAATTCCAGGAGGGAAGCCGAGAATATGA
  AGCTGAATTGGAGACGCAGCTGCAACAAATTGAAACCAGGAACAGAGACCTCCTGTCCGAAAATAACCGC
  CTTCGCATGGAGCTGGAAACCATCAAGGAGAAGTTTGAAGTGCAGCACTCTGAAGGCTACCGGCAGATCT
  CAGCCTTGGAGGATGACCTCGCGCAGACCAAAGCCATTAAAGACCAATTGCAGAAATACATCAGAGAGCT
  GGAGCAAGCAAATGACGACCTGGAAAGAGCCAAGCGCGCCACGATCATGTCTCTCGAAGACTTTGAGCAG
  CGCTTGAATCAGGCCATCGAAAGAAATGCCTTCCTGGAAAGTGAACTTGATGAAAAAGAGAATCTCCTGG
  AATCTGTTCAGAGACTGAAGGATGAAGCCAGAGATTTGCGGCAGGAACTGGCCGTGCAGCAGAAGCAGGA
  GAAACCCAGGATCCCCATGCCCAGCTCAGTGGAAGCTGAGAGGACAGACACAGCTGTGCAGGCCACGGGC
  TCCGTGCCGTCCACGCCCATTGCTCACCGAGGACCAAGCTCAAGTTTAAACACACCTGGGAGCTTCAGAC
  GTGGCCTGGACGACTCCACCGGGGGGACCCCCCTCACACCTGCGGCCCGGATATCAGCCCTCAACATTGT
  GGGAGACCTACTGCGGAAAGTCGGGGCACTGGAGTCCAAACTCGCTTCCTGCCGGAACCTCGTGTACGAT
  CAGTCCCCAAACCGAACAGGTGGCCCAGCCTCTGGGCGGAGCAGCAAGAACAGAGATGGCGGGGAGAGAC
  GGCCAAGCAGCACCAGCGTGCCTTTGGGTGATAAGGGGTTGGACACGAGTTGCCGCTGGTTGTCCAAATC
  AACAACCAGGTCGTCCAGCTCCTGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG227919 representing NM_001143979
  Red=Cloning site Green=Tags(s)
  
  MEDSGKTFSSEEEEANYWKDLAMTYKQRAENTQEELREFQEGSREYEAELETQLQQIETRNRDLLSENNR
  LRMELETIKEKFEVQHSEGYRQISALEDDLAQTKAIKDQLQKYIRELEQANDDLERAKRATIMSLEDFEQ
  RLNQAIERNAFLESELDEKENLLESVQRLKDEARDLRQELAVQQKQEKPRIPMPSSVEAERTDTAVQATG
  SVPSTPIAHRGPSSSLNTPGSFRRGLDDSTGGTPLTPAARISALNIVGDLLRKVGALESKLASCRNLVYD
  QSPNRTGGPASGRSSKNRDGGERRPSSTSVPLGDKGLDTSCRWLSKSTTRSSSSC
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001143979
• ORF Size: 1005 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001143979.1, NP_001137451.1
• RefSeq Size: 3936
• RefSeq ORF: 1008
• Locus ID: 54820
• Gene Summary: This gene encodes a member of the nuclear distribution E (NudE) family of proteins. The encoded protein is localized at the centrosome and interacts with other centrosome components as part of a multiprotein complex that regulates dynein function. This protein plays an essential role in microtubule organization, mitosis and neuronal migration. Mutations in this gene cause lissencephaly 4, a disorder characterized by lissencephaly, severe brain atrophy, microcephaly, and severe cognitive disability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2012]