EME1 (NM_001166131) Human Tagged ORF Clone

CAT#: RG228967

EME1 (GFP-tagged) - Human essential meiotic endonuclease 1 homolog 1 (S. pombe) (EME1), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: EME1
• Synonyms: MMS4L; SLX2A
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG228967 representing NM_001166131
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTAAAGAAGTCATCACCCTCACTGGATTCTGGTGATAGTGACTCTGAGGAGTTGCCAACATTTG
  CCTTTCTGAAGAAGGAACCATCTTCAACAAAGAGGAGACAGCCTGAAAGGGAAGAGAAGATTGTAGTGGT
  TGACATCTCAGATTGTGAAGCCTCCTGTCCTCCAGCACCAGAGTTATTTTCACCACCTGTCCCAGAAATA
  GCTGAAACTGTCACACAAACACAGCCAGTCAGGTTGCTAAGCAGTGAAAGTGAAGATGAAGAAGAATTTA
  TTCCTCTGGCTCAAAGGCTTACATGTAAGTTTCTGACCCACAAGCAACTGAGCCCTGAGGACTCTAGCTC
  CCCAGTTAAAAGTGTTTTGGATCATCAAAATAATGAAGGTGCATCATGTGACTGGAAAAAGCCCTTTCCA
  AAGATCCCTGAAGTTCCCCTCCATGATACCCCAGAGAGGAGTGCAGCAGATAACAAGGACCTGATCTTAG
  ATCCATGCTGTCAGCTTCCAGCCTACCTGTCTACCTGCCCTGGCCAGAGCAGCAGCTTGGCAGTAACCAA
  AACAAATTCTGACATCCTTCCACCCCAGAAGAAAACCAAGCCGAGTCAGAAGGTCCAGGGAAGAGGCTCA
  CACGGATGCCGGCAGCAGAGACAAGCAAGGCAGAAGGAAAGCACCCTGAGAAGACAGGAAAGAAAGAATG
  CAGCACTGGTTACCAGGATGAAAGCCCAGAGGCCAGAGGAATGCTTAAAACACATCATTGTAGTGCTGGA
  TCCAGTGCTCTTACAGATGGAAGGTGGGGGCCAGCTCCTAGGAGCACTGCAGACCATGGAGTGCCGCTGT
  GTGATTGAGGCGCAGGCTGTGCCTTGCAGTGTCACTTGGAGGAGAAGGGCTGGGCCGTCTGAGGACAGAG
  AGGACTGGGTGGAGGAGCCAACAGTACTGGTGTTGCTCCGGGCAGAGGCATTTGTGTCCATGATCGACAA
  TGGAAAGCAGGGAAGCCTGGACAGCACTATGAAAGGGAAGGAAACGCTTCAGGGCTTTGTAACTGACATC
  ACAGCAAAGACAGCAGGGAAAGCTCTGTCACTGGTGATTGTGGATCAGGAGAAATGCTTCAGCCTGGAGC
  TGCTGTTCTTTGATTTCCTCCCCTGCACCAGTGCTCAGAATCCTCCAAGAAGAGGGAAACAGGGAGCAAA
  TAAACAGACCAAGAAGCAGCAGCAGAGACAACCAGAGGCCAGCATAGGGTCCATGGTATCCAGGGTAGAC
  GCTGAAGAGGCATTGGTGGATCTGCAGCTACACACAGAAGCCCAGGCTCAAATTGTGCAGAGCTGGAAAG
  AGCTGGCCGACTTCACATGCGCATTCACAAAGGCTGTGGCTGAGGCGCCCTTCAAGAAGCTCCGAGATGA
  AACTACCTTCTCCTTCTGTCTGGAGAGTGACTGGGCTGGAGGGGTGAAGGTGGACCTTGCTGGCAGGGGA
  CTCGCACTAGTCTGGAGGAGACAGATTCAGCAGCTGAACCGAGTCAGCCTGGAAATGGCCAGTGCAGTTG
  TGAATGCCTATCCCTCCCCACAGCTCCTGGTACAGGCTTATCAGCAGTGTTTTTCGGATAAAGAACGCCA
  GAATTTGCTCGCAGACATACAGGTGCGCCGTGGGGAAGGTGTGACATCCACTTCTCGCCGCATTGGACCA
  GAACTATCCAGGCGTATCTACCTTCAGATGACCACTTTACAGCCACATCTCTCTTTAGATAGTGCTGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG228967 representing NM_001166131
  Red=Cloning site Green=Tags(s)
  
  MALKKSSPSLDSGDSDSEELPTFAFLKKEPSSTKRRQPEREEKIVVVDISDCEASCPPAPELFSPPVPEI
  AETVTQTQPVRLLSSESEDEEEFIPLAQRLTCKFLTHKQLSPEDSSSPVKSVLDHQNNEGASCDWKKPFP
  KIPEVPLHDTPERSAADNKDLILDPCCQLPAYLSTCPGQSSSLAVTKTNSDILPPQKKTKPSQKVQGRGS
  HGCRQQRQARQKESTLRRQERKNAALVTRMKAQRPEECLKHIIVVLDPVLLQMEGGGQLLGALQTMECRC
  VIEAQAVPCSVTWRRRAGPSEDREDWVEEPTVLVLLRAEAFVSMIDNGKQGSLDSTMKGKETLQGFVTDI
  TAKTAGKALSLVIVDQEKCFSLELLFFDFLPCTSAQNPPRRGKQGANKQTKKQQQRQPEASIGSMVSRVD
  AEEALVDLQLHTEAQAQIVQSWKELADFTCAFTKAVAEAPFKKLRDETTFSFCLESDWAGGVKVDLAGRG
  LALVWRRQIQQLNRVSLEMASAVVNAYPSPQLLVQAYQQCFSDKERQNLLADIQVRRGEGVTSTSRRIGP
  ELSRRIYLQMTTLQPHLSLDSAD
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001166131
• ORF Size: 1749 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001166131.1, NP_001159603.1
• RefSeq Size: 2371
• RefSeq ORF: 1752
• Locus ID: 146956
• Protein Pathways: Homologous recombination
• Gene Summary: This gene encodes a protein that complexes with methyl methanesulfonate-sensitive UV-sensitive 81 protein to form an endonuclease complex. The encoded protein interacts with specifc DNA structures including nicked Holliday junctions, 3'-flap structures and aberrant replication fork structures. This protein may be involved in repairing DNA damage and in maintaining genomic stability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]