HSPC302 (TBCK) (NM_001163437) Human Tagged ORF Clone

CAT#: RG229029

TBCK (GFP-tagged) - Human TBC1 domain containing kinase (TBCK), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TBCK
• Synonyms: HSPC302; IHPRF3; TBCKL
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG229029 representing NM_001163437
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTCCCCTGAAGGACGCTGAAATGGGAGCCTTTACCTTCTTTGCCTCGGCTCTGCCACATGATGTTT
  GTGGAAGCAATGGACTTCCTCTCACACCAAATTCCATCAAAATTTTAGGGCGCTTTCAAATCCTTAAAAC
  CATCACCCATCCCAGACTCTGCCAGTATGTGGATATTTCTAGGGGAAAGCATGAACGACTAGTGGTCGTG
  GCTGAACATTGTGAACGTAGTCTGGAAGACTTGCTTCGAGAAAGGAAACCTGTGAGCTGTTCAACGGTTT
  TGTGTATAGCATTTGAGGTTCTTCAGGGCTTGCAGTATATGAACAAACATGGTATAGTACACAGGGCATT
  GTCTCCTCATAATATCCTGTTGGACCGAAAGGGACATATTAAATTGGCTAAATTTGGACTTTATCACATG
  ACAGCTCATGGTGATGATGTTGATTTCCCAATAGGGTATCCCTCGTACTTGGCCCCTGAGGGAAGAAAAT
  TATTTCAGAGCTTGGATATTTCTGAAAGACTAAAATTTTTGCTTACTTTGGATTGTGTAGATGACACTTT
  AATAGTTCTGGCTGAAGAGCATGGTTGTTTGGACATTATAAAGGAGCTTCCTGAAACTGTGATAGATCTT
  TTGAATAAGTGCCTTACCTTCCATCCTTCTAAGAGGCCAACCCCAGATGAATTAATGAAGGACAAAGTAT
  TCAGTGAGGTATCACCTTTATATACCCCCTTTACCAAACCTGCCAGTCTGTTTTCATCTTCTCTGAGATG
  TGCTGATTTAACTCTGCCTGAGGATATCAGTCAGTTGTGTAAAGATATAAATAATGATTACCTGGCAGAA
  AGATCTATTGAAGAAGTGTATTACCTTTGGTGTTTGGCTGGAGGTGACTTGGAGAAAGAGCTTGTCAACA
  AGGAAATCATTCGATCCAAACCACCTATCTGCACACTCCCCAATTTTCTCTTTGAGGATGGTGAAAGCTT
  TGGACAAGGTCGAGATAGAAGCTCGCTTTTAGATGATACCACTGTGACATTGTCGTTATGCCAGCTAAGA
  AATAGATTGAAAGATGTTGGTGGAGAAGCATTTTACCCATTACTTGAAGATGACCAGTCTAATTTACCTC
  ATTCAAACAGCAATAATGAGTTGTCTGCAGCTGCCACGCTCCCTTTAATCATCAGAGAGAAGGATACAGA
  GTACCAACTAAATAGAATTATTCTCTTCGACAGGCTGCTAAAGGCTTATCCATATAAAAAAAACCAAATC
  TGGAAAGAAGCAAGAGTTGACATTCCTCCTCTTATGAGAGGTTTAACCTGGGCTGCTCTTCTGGGAGTTG
  AGGGAGCTATTCATGCCAAGTACGATGCAATTGATAAAGACACTCCAATTCCTACAGATAGACAAATTGA
  AGTGGATATTCCTCGCTGTCATCAGTACGATGAACTGTTATCATCACCAGAAGGTCATGCAAAATTTAGG
  CGTGTATTAAAAGCCTGGGTAGTGTCTCATCCTGATCTTGTGTATTGGCAAGGTCTTGACTCACTTTGTG
  CTCCATTCCTATATCTAAACTTCAATAATGAAGCCTTGGCTTATGCATGTATGTCTGCTTTTATTCCCAA
  ATACCTGTATAACTTCTTCTTAAAAGACAACTCACATGTAATACAAGAGTATCTGACTGTCTTCTCTCAG
  ATGATTGCATTTCATGATCCAGAGCTGAGTAATCATCTCAATGAGATTGGTTTCATTCCAGATCTCTATG
  CCATCCCTTGGTTTCTTACCATGTTTACTCATGTATTTCCACTACACAAAATTTTCCACCTCTGGGATAC
  CTTACTACTTGGGAATTCCTCTTTCCCATTCTGTATTGGAGTAGCAATTCTTCAGCAGCTGCGGGACCGG
  CTTTTGGCTAATGGCTTTAATGAGTGTATTCTTCTCTTCTCCGATTTACCAGAAATTGACATTGAACGCT
  GTGTGAGAGAATCTATCAACCTGTTTTGTTGGACTCCTAAAAGTGCTACTTACAGACAGCATGCTCAACC
  TCCAAAGCCATCTTCTGACAGCAGTGGAGGCAGAAGTTCGGCACCTTATTTCTCTGCTGAGTGTCCAGAT
  CCTCCAAAGACAGATCTGTCAAGAGAATCCATCCCATTAAATGACCTGAAGTCAGAAGTATCACCACGGA
  TTTCAGCAGAGGACCTGATTGACTTGTGTGAGCTCACAGTGACAGGCCACTTCAAAACACCCAGCAAGAA
  AACAAAGTCCAGTAAACCAAAGCTCCTGGTGGTTGACATCCGGAATAGTGAAGACTTTATTCGTGGTCAC
  ATTTCAGGAAGCATCAACATTCCATTCAGTGCTGCCTTCACTGCAGAAGGGGAGCTTACCCAGGGCCCTT
  ACACTGCTATGCTCCAGAACTTCAAAGGGAAGGTCATTGTCATCGTGGGGCATGTGGCAAAACACACAGC
  TGAGTTTGCAGCTCACCTTGTGAAGATGAAATATCCAAGAATCTGTATTCTAGATGGTGGCATTAATAAA
  ATAAAGCCAACAGGCCTCCTCACCATCCCATCTCCTCAAATA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG229029 representing NM_001163437
  Red=Cloning site Green=Tags(s)
  
  MFPLKDAEMGAFTFFASALPHDVCGSNGLPLTPNSIKILGRFQILKTITHPRLCQYVDISRGKHERLVVV
  AEHCERSLEDLLRERKPVSCSTVLCIAFEVLQGLQYMNKHGIVHRALSPHNILLDRKGHIKLAKFGLYHM
  TAHGDDVDFPIGYPSYLAPEGRKLFQSLDISERLKFLLTLDCVDDTLIVLAEEHGCLDIIKELPETVIDL
  LNKCLTFHPSKRPTPDELMKDKVFSEVSPLYTPFTKPASLFSSSLRCADLTLPEDISQLCKDINNDYLAE
  RSIEEVYYLWCLAGGDLEKELVNKEIIRSKPPICTLPNFLFEDGESFGQGRDRSSLLDDTTVTLSLCQLR
  NRLKDVGGEAFYPLLEDDQSNLPHSNSNNELSAAATLPLIIREKDTEYQLNRIILFDRLLKAYPYKKNQI
  WKEARVDIPPLMRGLTWAALLGVEGAIHAKYDAIDKDTPIPTDRQIEVDIPRCHQYDELLSSPEGHAKFR
  RVLKAWVVSHPDLVYWQGLDSLCAPFLYLNFNNEALAYACMSAFIPKYLYNFFLKDNSHVIQEYLTVFSQ
  MIAFHDPELSNHLNEIGFIPDLYAIPWFLTMFTHVFPLHKIFHLWDTLLLGNSSFPFCIGVAILQQLRDR
  LLANGFNECILLFSDLPEIDIERCVRESINLFCWTPKSATYRQHAQPPKPSSDSSGGRSSAPYFSAECPD
  PPKTDLSRESIPLNDLKSEVSPRISAEDLIDLCELTVTGHFKTPSKKTKSSKPKLLVVDIRNSEDFIRGH
  ISGSINIPFSAAFTAEGELTQGPYTAMLQNFKGKVIVIVGHVAKHTAEFAAHLVKMKYPRICILDGGINK
  IKPTGLLTIPSPQI
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163437
• ORF Size: 2562 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001163437.1, NP_001156909.1
• RefSeq Size: 3424
• RefSeq ORF: 2565
• Locus ID: 93627
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a protein that contains a protein kinase domain, a Rhodanase-like domain and the Tre-2/Bub2/Cdc16 (TBC) domain. The encoded protein is thought to play a role in actin organization, cell growth and cell proliferation by regulating the mammalian target of the rapamycin (mTOR) signaling pathway. This protein may also be involved in the transcriptional regulation of the components of the mTOR complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]