Kir7.1 (KCNJ13) (NM_001172417) Human Tagged ORF Clone

CAT#: RG229820

KCNJ13 (GFP-tagged) - Human potassium inwardly-rectifying channel, subfamily J, member 13 (KCNJ13), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: KCNJ13
• Synonyms: KIR1.4; KIR7.1; LCA16; SVD
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG229820 representing NM_001172417
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGGTGATCTGGAACTAGATCATGATGCCCCACCTGAAAACCACACTATCTGTGTCAAGTATATCA
  CCAGTTTCACAGCTGCATTCTCCTTCTCCCTGGAGACACAACTCACAATTGGTTATGGTACCATGTTCCC
  CAGTGGTGACTGTCCAAGTGCAATCGCCTTACTTGCCATACAAATGCTCCTAGGCCTCATGCTAGAGGCT
  TTTATCACAGGTGCTTTTGTGGCGAAGATTGCCCGGCCAAAAAATCGAGCTTTTTCAATTCGCTTTACTG
  ACACAGCAGTAGTAGCTCACATGGATGGCAAACCTAATCTTATCTTCCAAGTGGCCAACACCCGACCTAG
  CCCTCTAACCAGTGTCCGGGTCTCAGCTGTACTCTATCAGGAAAGAGAAAATGGCAAACTCTACCAGACC
  AGTGTGGATTTCCACCTTGATGGCATCAGTTCTGACGAATGTCCATTCTTCATCTTTCCACTAACGTACT
  ATCACTCCATTACACCATCAAGTCCTCTGGCTACTCTGCTCCAGCATGAAAATCCTTCTCACTTTGAATT
  AGTTGTATTCCTTTCAGCAATGCAGGAGGGCACTGGAGAAATATGCCAAAGGAGGACATCCTACCTACCG
  TCTGAAATCATGTTACATCACTGTTTTGCATCTCTGTTGACCCGAGGTTCCAAAGGTGAATATCAAATCA
  AGATGGAGAATTTTGACAAGACTGTCCCTGAATTTCCAACTCCTCTGGTTTCTAAAAGCCCAAACAGGAC
  TGACCTGGATATCCACATCAATGGACAAAGCATTGACAATTTTCAGATCTCTGAAACAGGACTGACAGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG229820 representing NM_001172417
  Red=Cloning site Green=Tags(s)
  
  MNGDLELDHDAPPENHTICVKYITSFTAAFSFSLETQLTIGYGTMFPSGDCPSAIALLAIQMLLGLMLEA
  FITGAFVAKIARPKNRAFSIRFTDTAVVAHMDGKPNLIFQVANTRPSPLTSVRVSAVLYQERENGKLYQT
  SVDFHLDGISSDECPFFIFPLTYYHSITPSSPLATLLQHENPSHFELVVFLSAMQEGTGEICQRRTSYLP
  SEIMLHHCFASLLTRGSKGEYQIKMENFDKTVPEFPTPLVSKSPNRTDLDIHINGQSIDNFQISETGLTE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001172417
• ORF Size: 840 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001172417.1, NP_001165888.1
• RefSeq Size: 3376 bp
• RefSeq ORF: 843 bp
• Locus ID: 3769
• Cytogenetics: 2q37.1
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Gene Summary: 'This gene encodes a member of the inwardly rectifying potassium channel family of proteins. Members of this family form ion channel pores that allow potassium ions to pass into a cell. The encoded protein belongs to a subfamily of low signal channel conductance proteins that have a low dependence on potassium concentration. Mutations in this gene are associated with snowflake vitreoretinal degeneration. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2010]'