SIGLEC6 (NM_001177547) Human Tagged ORF Clone

CAT#: RG230004

SIGLEC6 (GFP-tagged) - Human sialic acid binding Ig-like lectin 6 (SIGLEC6), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SIGLEC6
• Synonyms: CD33L; CD33L1; CD33L2; CD327; CDW327; OBBP1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230004 representing NM_001177547
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGGAGCCCAGGAAGCCTCCGCCTCAGAGATGCTACCGCTGCTGCTGCCCCTGCTGTGGGCAGCCT
  CGTACTATGGTTATGGCTACTGGTTCCTGGAAGGGGCTGATGTTCCAGTGGCCACAAACGACCCAGACGA
  AGAAGTGCAGGAGGAGACCCGGGGCCGATTCCACCTCCTCTGGGATCCCAGAAGGAAGAACTGCTCCCTG
  AGCATCAGAGATGCCCGGAGGAGGGACAATGCTGCATACTTCTTTCGGTTGAAGTCCAAATGGATGAAAT
  ACGGTTATACATCTTCCAAGCTCTCTGTGCGTGTGATGGCCCTGACCCACAGGCCCAACATCTCCATCCC
  AGGGACCCTGGAGTCTGGCCATCCCAGCAATCTGACCTGCTCTGTGCCCTGGGTCTGTGAGCAGGGGACG
  CCCCCCATCTTCTCCTGGATGTCAGCTGCCCCCACCTCCCTGGGCCCCAGGACCACCCAGTCCTCGGTGC
  TCACAATCACCCCACGGCCCCAGGACCACAGCACCAACCTCACCTGTCAGGTGACGTTCCCTGGAGCCGG
  TGTGACCATGGAGAGAACCATCCAGCTCAATGTCTCCTCCTTCAAAATCCTGCAAAACACCTCGTCCCTC
  CCTGTCCTGGAGGGCCAGGCTCTGCGGCTGCTCTGTGATGCTGACGGCAACCCCCCTGCACACCTGAGCT
  GGTTCCAGGGCTTCCCCGCCCTGAACGCCACCCCCATCTCCAATACCGGGGTCCTGGAGCTGCCTCAAGT
  AGGGTCTGCAGAAGAAGGAGATTTCACCTGCCGTGCTCAGCATCCTCTGGGCTCCCTGCAAATCTCTCTG
  AGTCTCTTTGTGCATTGGAAACCAGAAGGCAGGGCTGGTGGTGTCCTGGGAGCAGTCTGGGGAGCTAGCA
  TCACAACCCTGGTTTTCCTCTGTGTTTGCTTCATCTTCAGAGTGAAGACTAGAAGGAAGAAAGCAGCCCA
  GCCAGTGCAAAACACGGATGATGTGAACCCCGTCATGGTCTCAGGCTCCAGGGGTCATCAGCACCAGTTC
  CAGACAGGCATAGTTTCAGACCACCCTGCTGAGGCTGGCCCCATCTCAGAAGATGAGCAGGAGCTCCACT
  ACGCTGTCCTACACTTCCACAAGGTGCAACCTCAGGAACCAAAGGTCACCGACACTGAGTACTCAGAAAT
  CAAGATACACAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230004 representing NM_001177547
  Red=Cloning site Green=Tags(s)
  
  MQGAQEASASEMLPLLLPLLWAASYYGYGYWFLEGADVPVATNDPDEEVQEETRGRFHLLWDPRRKNCSL
  SIRDARRRDNAAYFFRLKSKWMKYGYTSSKLSVRVMALTHRPNISIPGTLESGHPSNLTCSVPWVCEQGT
  PPIFSWMSAAPTSLGPRTTQSSVLTITPRPQDHSTNLTCQVTFPGAGVTMERTIQLNVSSFKILQNTSSL
  PVLEGQALRLLCDADGNPPAHLSWFQGFPALNATPISNTGVLELPQVGSAEEGDFTCRAQHPLGSLQISL
  SLFVHWKPEGRAGGVLGAVWGASITTLVFLCVCFIFRVKTRRKKAAQPVQNTDDVNPVMVSGSRGHQHQF
  QTGIVSDHPAEAGPISEDEQELHYAVLHFHKVQPQEPKVTDTEYSEIKIHK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001177547
• ORF Size: 1203 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177547.1, NP_001171018.1
• RefSeq Size: 1966 bp
• RefSeq ORF: 1206 bp
• Locus ID: 946
• Cytogenetics: 19q13.41
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Gene Summary: 'This gene encodes a member of the SIGLEC (sialic acid binding immunoglobulin-like lectin) family of proteins. The encoded transmembrane receptor binds sialyl-TN glycans and leptin. Placental expression of the encoded protein is upregulated in preeclampsia. [provided by RefSeq, Jul 2016]'