TXNRD3 (NM_001173513) Human Tagged ORF Clone

CAT#: RG230340

TXNRD3 (GFP-tagged) - Human thioredoxin reductase 3 (TXNRD3), transcript variant 2, (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Symbol: TXNRD3
• Synonyms: TGR; TR2; TR2IT1; TRXR3; TXNRD3IT1; TXNRD3NB; TXNRD3NT1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230340 representing NM_001173513
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  CTGGAGCGGTCGCCGCCGCAGTCGCCCGGGCCGGGAAAGGCGGGCGATGCCCCCAACCGCCGCTCGGGCC
  ATGTCCGAGGGGCGCGCGTGTTGTCGCCGCCGGGGCGCCGTGCCCGCCTGTCGTCCCCCGGGCCCAGCCG
  CTCGTCCGAGGCCCGCGAGGAGCTGCGCCGCCACCTCGTGGGCCTCATCGAGCGCAGCCGGGTGGTGATC
  TTCAGCAAGAGCTACTGTCCCCATAGTACTCGGGTGAAAGAACTCTTTTCTTCTTTGGGAGTCGAATGTA
  ATGTCTTGGAACTTGATCAAGTTGATGATGGGGCCAGGGTTCAAGAAGTGCTGTCAGAAATCACTAATCA
  GAAAACTGTGCCCAATATTTTCGTGAATAAAGTGCATGTAGGTGGATGTGACCAAACTTTCCAGGCATAT
  CAGAGTGGTTTGTTACAGAAGCTCCTTCAGGAAGATTTGGCATATGATTATGATCTCATCATCATCGGTG
  GTGGTTCTGGAGGCCTTTCATGTGCGAAGGAAGCTGCCATTTTGGGAAAGAAAGTTATGGTGCTAGACTT
  TGTTGTCCCGTCACCTCAGGGCACATCCTGGGGTCTTGGTGGCACTTGTGTAAATGTAGGTTGTATTCCT
  AAGAAATTGATGCATCAGGCTGCCCTTTTGGGGCAGGCATTATGTGACTCAAGGAAATTTGGCTGGGAAT
  ATAATCAACAAGTGAGGCACAACTGGGAGACAATGACAAAAGCGATTCAGAACCACATCAGCTCTCTAAA
  CTGGGGCTACAGGTTGTCTCTGAGGGAAAAGGCTGTGGCCTATGTCAATTCCTATGGAGAATTTGTTGAA
  CATCATAAAATAAAGGCAACCAATAAAAAAGGACAGGAGACTTATTATACTGCTGCACAGTTTGTCATAG
  CAACGGGTGAAAGGCCACGGTATTTAGGAATCCAAGGAGATAAAGAATACTGTATTACTAGTGATGACCT
  TTTTTCTCTGCCTTATTGCCCTGGCAAAACATTAGTGGTGGGTGCCTCTTATGTTGCCCTGGAGTGTGCA
  GGGTTTCTGGCTGGCTTTGGCCTAGATGTCACAGTTATGGTACGCTCAATCCTTCTCCGTGGCTTCGACC
  AAGAAATGGCAGAAAAAGTGGGTTCCTACATGGAGCAGCATGGTGTGAAGTTCCTACGGAAATTCATACC
  TGTGATGGTTCAACAGTTGGAGAAAGGTTCACCTGGAAAGCTGAAAGTGTTGGCTAAATCCACTGAAGGA
  ACAGAAACAATTGAAGGAGTCTATAACACAGTTTTGTTAGCTATTGGTCGTGACTCCTGTACAAGGAAAA
  TAGGCTTGGAGAAGATTGGTGTCAAAATTAATGAGAAGAGTGGAAAAATACCTGTAAATGATGTGGAACA
  GACCAATGTGCCATATGTCTATGCTGTTGGTGATATTTTGGAGGATAAGCCAGAGCTCACTCCTGTCGCC
  ATACAGTCAGGCAAGCTGCTAGCTCAGAGACTTTTTGGGGCCTCTTTAGAAAAGATATATCATACTTTGT
  TCTGGCCTCTTGAATGGACAGTAGCTGGCAGAGAGAACAACACTTGTTACGCAAAGATAATCTGCAATAA
  ATTCGACCATGATCGGGTGATAGGATTTCATATTCTTGGACCAAACGCCGGTGAGGTTACCCAAGGATTT
  GCAGCTGCAATGAAATGTGGGCTCACAAAACAGCTACTTGATGACACCATTGGAATTCACCCCACATGTG
  GGGAGGTGTTCACGACTTTGGAAATCACAAAGTCGTCAGGACTAGACATCACTCAGAAAGGCTGCTGAGG
  C
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230340 representing NM_001173513
  Red=Cloning site Green=Tags(s)
  
  LERSPPQSPGPGKAGDAPNRRSGHVRGARVLSPPGRRARLSSPGPSRSSEAREELRRHLVGLIERSRVVI
  FSKSYCPHSTRVKELFSSLGVECNVLELDQVDDGARVQEVLSEITNQKTVPNIFVNKVHVGGCDQTFQAY
  QSGLLQKLLQEDLAYDYDLIIIGGGSGGLSCAKEAAILGKKVMVLDFVVPSPQGTSWGLGGTCVNVGCIP
  KKLMHQAALLGQALCDSRKFGWEYNQQVRHNWETMTKAIQNHISSLNWGYRLSLREKAVAYVNSYGEFVE
  HHKIKATNKKGQETYYTAAQFVIATGERPRYLGIQGDKEYCITSDDLFSLPYCPGKTLVVGASYVALECA
  GFLAGFGLDVTVMVRSILLRGFDQEMAEKVGSYMEQHGVKFLRKFIPVMVQQLEKGSPGKLKVLAKSTEG
  TETIEGVYNTVLLAIGRDSCTRKIGLEKIGVKINEKSGKIPVNDVEQTNVPYVYAVGDILEDKPELTPVA
  IQSGKLLAQRLFGASLEKIYHTLFWPLEWTVAGRENNTCYAKIICNKFDHDRVIGFHILGPNAGEVTQGF
  AAAMKCGLTKQLLDDTIGIHPTCGEVFTTLEITKSSGLDITQKGC*G
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001173513
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info The expression of this clone is not guaranteed due to the nature of selenoproteins.
• OTI Annotation: This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins.

Reference Data

• RefSeq: NM_001173513.1, NP_001166984.1
• RefSeq Size: 2811
• RefSeq ORF: 1824
• Locus ID: 114112
• Gene Summary: The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes the third TrxR, which unlike the other two isozymes, contains an additional N-terminal glutaredoxin (Grx) domain, and shows highest expression in testis. The Grx domain allows this isozyme to participate in both Trx and glutathione systems. It functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. Experimental evidence suggests the use of a non-AUG (CUG) codon as a translation initiation codon (PMID:20018845). [provided by RefSeq, Aug 2017]