Transcription termination factor 1 (TTF1) (NM_001205296) Human Tagged ORF Clone
CAT#: RG232638
- TrueORF®
TTF1 (GFP-tagged) - Homo sapiens transcription termination factor, RNA polymerase I (TTF1), transcript variant 2
"NM_001205296" in other vectors (2)
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Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | TTF1 |
Synonyms | TTF-1; TTF-I |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG232638 representing NM_001205296
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACCGGGACGACTTGGAACGGTTTAAGGAATTTAAAGCACAAGGTGTCGCTATTAAATTTGGCAAGT TTTCTGTAAAGGAAAATAAGCAGTTAGAGAAAAATGTGGAAGACTTTCTAGCCCTGACAGGCATTGAGAG TGCAGACAAGCTGCTGTACACGGACAGATATCCTGAGGAAAAATCTGTGATCACCAACTTAAAAAGGAGA TACTCGTTTAGATTACACATTGGTAGGAACATTGCCCGGCCCTGGAAACTTATATACTATCGAGCAAAGA AGATGTTCGATGTCAACAATTACAAAGGCAGGTATAGCGAAGGAGATACTGAGAAGTTAAAGATGTACCA TTCTCTCCTTGGGAATGACTGGAAGACGATTGGTGAGATGGTGGCCCGAAGTAGCCTCTCCGTGGCCCTC AAGTTCTCACAGATCAGCAGTCAAAGAAATCGTGGTGCTTGGAGTAAGTCTGAAACCCGGAAACTAATCA AGGCTGTCGAAGAAGTGATTCTGAAGAAGATGTCTCCCCAGGAGTTAAAAGAGGTGGATTCCAAACTCCA AGAAAATCCTGAAAGTTGCCTATCAATTGTTCGGGAAAAACTCTACAAGGGCATATCTTGGGTAGAAGTA GAAGCTAAAGTGCAAACCAGAAATTGGATGCAGTGTAAAAGTAAGTGGACAGAAATTCTAACCAAGAGGA TGACTAATGGTCGGCGTATCTACTATGGCATGAATGCCCTGCGGGCCAAGGTCAGCCTTATTGAAAGGTT GTATGAAATAAATGTGGAAGATACTAATGAAATAGACTGGGAAGATCTTGCTAGTGCCATAGGTGATGTT CCTCCATCTTACGTTCAAACTAAATTTTCTAGGCTGAAAGCTGTCTATGTTCCATTTTGGCAGAAAAAGA CTTTTCCAGAGATCATCGACTACCTTTATGAGACGACTCTACCTTTGCTGAAGGAAAAGTTAGAAAAAAT GATGGAGAAAAAAGGCACTAAAATCCAGACTCCTGCAGCACCCAAGCAAGTTTTCCCATTTCGAGACATC TTTTATTATGAAGACGATAGTGAAGGAGAGGACATAGAAAAAGAAAGCGAAGGCCAGGCGCCATGCATGG CTCACGCCTGTAATTCCAGTACTTTGGGAGGCCAAGGCCGGTGGATCATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG232638 representing NM_001205296
Red=Cloning site Green=Tags(s) MYRDDLERFKEFKAQGVAIKFGKFSVKENKQLEKNVEDFLALTGIESADKLLYTDRYPEEKSVITNLKRR YSFRLHIGRNIARPWKLIYYRAKKMFDVNNYKGRYSEGDTEKLKMYHSLLGNDWKTIGEMVARSSLSVAL KFSQISSQRNRGAWSKSETRKLIKAVEEVILKKMSPQELKEVDSKLQENPESCLSIVREKLYKGISWVEV EAKVQTRNWMQCKSKWTEILTKRMTNGRRIYYGMNALRAKVSLIERLYEINVEDTNEIDWEDLASAIGDV PPSYVQTKFSRLKAVYVPFWQKKTFPEIIDYLYETTLPLLKEKLEKMMEKKGTKIQTPAAPKQVFPFRDI FYYEDDSEGEDIEKESEGQAPCMAHACNSSTLGGQGRWII TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001205296 |
ORF Size | 1170 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001205296.1, NP_001192225.1 |
RefSeq Size | 1780 bp |
RefSeq ORF | 1173 bp |
Locus ID | 7270 |
Cytogenetics | 9q34.13 |
Gene Summary | 'This gene encodes a transcription termination factor that is localized to the nucleolus and plays a critical role in ribosomal gene transcription. The encoded protein mediates the termination of RNA polymerase I transcription by binding to Sal box terminator elements downstream of pre-rRNA coding regions. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. This gene shares the symbol/alias 'TFF1' with another gene, NK2 homeobox 1, also known as thyroid transcription factor 1, which plays a role in the regulation of thyroid-specific gene expression. [provided by RefSeq, Apr 2011]' |
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