PPP2R2C (NM_001206996) Human Tagged ORF Clone

CAT#: RG232707

PPP2R2C (GFP-tagged) - Homo sapiens protein phosphatase 2, regulatory subunit B, gamma (PPP2R2C), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PPP2R2C
• Synonyms: B55-GAMMA; B55gamma; IMYPNO; IMYPNO1; PR52; PR55G
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG232707 representing NM_001206996
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAAAGAGAGACACGGCTGACATCATCTCTACCGTTGAGTTCAACCACACGGGAGAGCTGCTGGCCA
  CAGGTGACAAGGGCGGCCGGGTCGTCATCTTCCAGCGGGAACCAGAGAGTAAAAATGCGCCCCACAGCCA
  GGGCGAATACGACGTGTACAGCACTTTCCAGAGCCACGAGCCGGAGTTTGACTATCTCAAGAGCCTGGAG
  ATAGAGGAGAAGATCAACAAGATCAAGTGGCTCCCACAGCAGAACGCCGCCCACTCACTCCTGTCCACCA
  ACGATAAAACTATCAAATTATGGAAGATTACCGAACGAGATAAAAGGCCCGAAGGATACAACCTGAAGGA
  TGAAGAGGGGAAACTTAAGGACCTGTCCACGGTGACGTCACTGCAGGTGCCAGTGCTGAAGCCCATGGAT
  CTGATGGTGGAGGTGAGCCCTCGGAGGATCTTTGCCAATGGCCACACCTACCACATCAACTCCATCTCCG
  TCAACAGTGACTGCGAGACCTACATGTCGGCGGATGACCTGCGCATCAACCTCTGGCACCTGGCCATCAC
  CGACAGGAGCTTCAACATCGTGGACATCAAGCCGGCCAACATGGAGGACCTTACGGAGGTGATCACAGCA
  TCTGAGTTCCATCCGCACCACTGCAACCTCTTCGTCTACAGCAGCAGCAAGGGCTCCCTGCGGCTCTGCG
  ACATGCGGGCAGCTGCCCTGTGTGACAAGCATTCCAAGCTCTTTGAAGAGCCTGAGGACCCCAGTAACCG
  CTCATTCTTCTCGGAAATCATCTCCTCCGTGTCCGACGTGAAGTTCAGCCACAGCGGCCGCTACATGCTC
  ACCCGGGACTACCTTACAGTCAAGGTCTGGGACCTGAACATGGAGGCAAGACCCATAGAGACCTACCAGG
  TCCATGACTACCTTCGGAGCAAGCTCTGTTCCCTGTACGAGAACGACTGCATTTTCGACAAGTTTGAATG
  TGCCTGGAACGGGAGCGACAGCGTCATCATGACCGGGGCCTACAACAACTTCTTCCGCATGTTCGATCGG
  AACACCAAGCGGGACGTGACCCTGGAGGCCTCGAGGGAAAGCAGCAAGCCCCGGGCTGTGCTCAAGCCAC
  GGCGCGTGTGCGTGGGGGGCAAGCGCCGGCGTGATGACATCAGTGTGGACAGCTTGGACTTCACCAAGAA
  GATCCTGCACACGGCCTGGCACCCGGCTGAGAACATCATTGCCATCGCCGCCACCAACAACCTGTACATC
  TTCCAGGACAAGGTAAACTCTGACATGCAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG232707 representing NM_001206996
  Red=Cloning site Green=Tags(s)
  
  MGKRDTADIISTVEFNHTGELLATGDKGGRVVIFQREPESKNAPHSQGEYDVYSTFQSHEPEFDYLKSLE
  IEEKINKIKWLPQQNAAHSLLSTNDKTIKLWKITERDKRPEGYNLKDEEGKLKDLSTVTSLQVPVLKPMD
  LMVEVSPRRIFANGHTYHINSISVNSDCETYMSADDLRINLWHLAITDRSFNIVDIKPANMEDLTEVITA
  SEFHPHHCNLFVYSSSKGSLRLCDMRAAALCDKHSKLFEEPEDPSNRSFFSEIISSVSDVKFSHSGRYML
  TRDYLTVKVWDLNMEARPIETYQVHDYLRSKLCSLYENDCIFDKFECAWNGSDSVIMTGAYNNFFRMFDR
  NTKRDVTLEASRESSKPRAVLKPRRVCVGGKRRRDDISVDSLDFTKKILHTAWHPAENIIAIAATNNLYI
  FQDKVNSDMH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001206996
• ORF Size: 1290 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001206996.1, NP_001193925.1
• RefSeq Size: 4315 bp
• RefSeq ORF: 1293 bp
• Locus ID: 5522
• Cytogenetics: 4p16.1
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Tight junction
• Gene Summary: 'The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a gamma isoform of the regulatory subunit B55 subfamily. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]'