PACAP receptor (ADCYAP1R1) (NM_001199637) Human Tagged ORF Clone

CAT#: RG232738

ADCYAP1R1 (GFP-tagged) - Homo sapiens adenylate cyclase activating polypeptide 1 (pituitary) receptor type I (ADCYAP1R1), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ADCYAP1R1
• Synonyms: PAC1; PAC1R; PACAPR; PACAPRI
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG232738 representing NM_001199637
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGTGTCGTGCACGTTTCCCTGGCTGCTCTCCTCCTGCTGCCTATGGCCCCTGCCATGCATTCTG
  ACTGCATCTTCAAGAAGGAGCAAGCCATGTGCCTGGAGAAGATCCAGAGGGCCAATGAGCTGATGGGCTT
  CAATGATTCCTCTCCAGGCTGTCCTGGGATGTGGGACAACATCACGTGTTGGAAGCCCGCCCATGTGGGT
  GAGATGGTCCTGGTCAGCTGCCCTGAGCTCTTCCGAATCTTCAACCCAGACCAAGACATGGGAGTGGTGA
  GCCGGAACTGCACGGAGGATGGCTGGTCGGAACCCTTCCCTCATTACTTTGATGCCTGTGGGTTTGATGA
  ATATGAATCTGAGACTGGGGACCAGGATTATTACTACCTGTCAGTGAAGGCCCTCTACACGGTTGGCTAC
  AGCACATCCCTCGTCACCCTCACCACTGCCATGGTCATCCTTTGTCGCTTCCGGAAGCTGCACTGCACAC
  GCAACTTCATCCACATGAACCTGTTTGTGTCGTTCATGCTGAGGGCGATCTCCGTCTTCATCAAAGACTG
  GATTCTGTATGCGGAGCAGGACAGCAACCACTGCTTCATCTCCACTGTGGAATGTAAGGCCGTCATGGTT
  TTCTTCCACTACTGTGTTGTGTCCAACTACTTCTGGCTGTTCATCGAGGGCCTGTACCTCTTCACTCTGC
  TGGTGGAGACCTTCTTCCCTGAAAGGAGATACTTCTACTGGTACACCATCATTGGCTGGGGGACCCCAAC
  TGTGTGTGTGACAGTGTGGGCTACGCTGAGACTCTACTTTGATGACACAGGCTGCTGGGATATGAATGAC
  AGCACAGCTCTGTGGTGGGTGATCAAAGGCCCTGTGGTTGGCTCTATCATGGTTAACTTTGTGCTTTTTA
  TTGGCATTATCGTCATCCTTGTGCAGAAACTTCAGTCTCCAGACATGGGAGGCAATGAGTCCAGCATCTA
  CTTGCGACTGGCCCGGTCCACCCTGCTGCTCATCCCACTATTCGGAATCCACTACACAGTATTTGCCTTC
  TCCCCAGAGAATGTCAGCAAAAGGGAAAGACTCGTGTTTGAGCTGGGGCTGGGCTCCTTCCAGGGCTTTG
  TGGTGGCTGTTCTCTACTGTTTTCTGAATGGTGAGGTACAAGCGGAGATCAAGCGAAAATGGCGAAGCTG
  GAAGGTGAACCGTTACTTCGCTGTGGACTTCAAGCACCGACACCCGTCTCTGGCCAGCAGTGGGGTGAAT
  GGGGGCACCCAGCTCTCCATCCTGAGCAAGAGCAGCTCCCAAATCCGCATGTCTGGCCTCCCTGCTGACA
  ATCTGGCCACC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG232738 representing NM_001199637
  Red=Cloning site Green=Tags(s)
  
  MAGVVHVSLAALLLLPMAPAMHSDCIFKKEQAMCLEKIQRANELMGFNDSSPGCPGMWDNITCWKPAHVG
  EMVLVSCPELFRIFNPDQDMGVVSRNCTEDGWSEPFPHYFDACGFDEYESETGDQDYYYLSVKALYTVGY
  STSLVTLTTAMVILCRFRKLHCTRNFIHMNLFVSFMLRAISVFIKDWILYAEQDSNHCFISTVECKAVMV
  FFHYCVVSNYFWLFIEGLYLFTLLVETFFPERRYFYWYTIIGWGTPTVCVTVWATLRLYFDDTGCWDMND
  STALWWVIKGPVVGSIMVNFVLFIGIIVILVQKLQSPDMGGNESSIYLRLARSTLLLIPLFGIHYTVFAF
  SPENVSKRERLVFELGLGSFQGFVVAVLYCFLNGEVQAEIKRKWRSWKVNRYFAVDFKHRHPSLASSGVN
  GGTQLSILSKSSSQIRMSGLPADNLAT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001199637
• ORF Size: 1341 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001199637.1, NP_001186566.1
• RefSeq Size: 6446 bp
• RefSeq ORF: 1344 bp
• Locus ID: 117
• Cytogenetics: 7p14.3
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: 'This gene encodes type I adenylate cyclase activating polypeptide receptor, which is a membrane-associated protein and shares significant homology with members of the glucagon/secretin receptor family. This receptor mediates diverse biological actions of adenylate cyclase activating polypeptide 1 and is positively coupled to adenylate cyclase. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Dec 2010]'