CYP4F3 (NM_001199208) Human Tagged ORF Clone

CAT#: RG234339

CYP4F3 (GFP-tagged) - Homo sapiens cytochrome P450, family 4, subfamily F, polypeptide 3 (CYP4F3), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CYP4F3
• Synonyms: CPF3; CYP4F; CYPIVF3; LTB4H
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG234339 representing NM_001199208
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCACAGCTGAGCCTGTCCTCGCTGGGCCTTTGGCCAATGGCAGCATCCCCGTGGCTGCTCCTGCTGC
  TGGTTGGGGCCTCCTGGCTCCTGGCCCGCATCCTGGCCTGGACCTATACCTTCTATGACAACTGCTGCCG
  CCTCCGGTGTTTCCCGCAACCCCCGAAACGGAATTGGTTCTTGGGTCACCTGGGCCTGGTCACCCCCACG
  GAGCAGGGCATGAGGGTCCTGACTCAGCTGGTGGCCACCTACCCCCAGGGCTTTAAGGTCTGGATGGGCC
  CCATCTTCCCCGTCATCCGTTTTTGCCACCCCAACATCATCCGGTCTGTCATCAACGCCTCAGCTGCCAT
  TGTACCAAAGGACAAGGTCTTCTACAGCTTCCTGAAGCCCTGGCTGGGGGATGGGCTCCTGCTGAGTGCT
  GGTGAAAAGTGGAGCCGCCACCGTCGGATGCTGACGCCTGCCTTCCATTTCAACATCCTGAAGCCCTATA
  TGAAGATTTTCAATGAGAGTGTGAACATCATGCATGCCAAGTGGCAGCTCCTGGCCTCAGAGGGTAGTGC
  CCGTCTGGACATGTTTGAGCACATCAGCCTCATGACCTTGGACAGTCTGCAGAAATGTGTCTTCAGCTTT
  GACAGCCATTGCCAGGAGAAGCCCAGTGAATATATTGCCGCCATCTTGGAGCTCAGTGCCCTTGTGACAA
  AAAGACACCAGCAGATCCTCCTGTACATAGACTTCCTGTATTATCTCACCCCTGATGGGCAGCGTTTCCG
  CAGGGCCTGCCGCCTGGTGCACGACTTCACAGATGCCGTCATCCAGGAGCGGCGCCGCACCCTCCCTAGC
  CAGGGTGTTGATGACTTCCTCCAAGCCAAGGCCAAATCCAAGACTTTGGACTTCATTGATGTACTCCTGC
  TGAGCAAGGATGAAGATGGGAAGAAGTTGTCCGATGAGGACATAAGAGCAGAAGCTGACACCTTTATGTT
  TGAGGGCCATGACACCACAGCCAGTGGTCTCTCCTGGGTCCTGTACCACCTTGCAAAGCACCCGGAATAC
  CAGGAGCGCTGTCGGCAGGAGGTGCAAGAGCTTCTGAAGGACCGTGAGCCTAAAGAGATTGAATGGGACG
  ACCTGGCCCAGCTGCCCTTCCTGACCATGTGCATTAAGGAGAGCCTGAGGCTGCATCCCCCAGTCCCTGC
  CGTCTCTCGCTGCTGCACCCAAGACATTGTGCTCCCAGACGGCCGGGTCATCCCCAAAGGCATTATCTGC
  CTCATCAGTGTTTTTGGAACCCATCACAACCCAGCCGTGTGGCCGGACCCTGAGGTCTATGACCCCTTTC
  GCTTTGACCCAAAGAACATCAAGGAGAGGTCACCTCTGGCTTTTATTCCCTTCTCAGCAGGGCCCAGGAA
  CTGCATCGGGCAGGCGTTCGCGATGGCGGAGATGAAGGTGGTCCTGGGGCTCACGCTGCTGCGCTTCCGC
  GTCCTGCCTGACCACACCGAGCCCCGCAGGAAGCCGGAGCTGGTCCTGCGCGCAGAGGGCGGACTTTGGC
  TGCGGGTGGAGCCCCTGAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG234339 representing NM_001199208
  Red=Cloning site Green=Tags(s)
  
  MPQLSLSSLGLWPMAASPWLLLLLVGASWLLARILAWTYTFYDNCCRLRCFPQPPKRNWFLGHLGLVTPT
  EQGMRVLTQLVATYPQGFKVWMGPIFPVIRFCHPNIIRSVINASAAIVPKDKVFYSFLKPWLGDGLLLSA
  GEKWSRHRRMLTPAFHFNILKPYMKIFNESVNIMHAKWQLLASEGSARLDMFEHISLMTLDSLQKCVFSF
  DSHCQEKPSEYIAAILELSALVTKRHQQILLYIDFLYYLTPDGQRFRRACRLVHDFTDAVIQERRRTLPS
  QGVDDFLQAKAKSKTLDFIDVLLLSKDEDGKKLSDEDIRAEADTFMFEGHDTTASGLSWVLYHLAKHPEY
  QERCRQEVQELLKDREPKEIEWDDLAQLPFLTMCIKESLRLHPPVPAVSRCCTQDIVLPDGRVIPKGIIC
  LISVFGTHHNPAVWPDPEVYDPFRFDPKNIKERSPLAFIPFSAGPRNCIGQAFAMAEMKVVLGLTLLRFR
  VLPDHTEPRRKPELVLRAEGGLWLRVEPLS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001199208
• ORF Size: 1560 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001199208.1, NP_001186137.1
• RefSeq Size: 2988
• RefSeq ORF: 1563
• Locus ID: 4051
• Protein Families: Druggable Genome, P450, Transmembrane
• Protein Pathways: Arachidonic acid metabolism, Metabolic pathways
• Gene Summary: This gene, CYP4F3, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. The enzyme starts the process of inactivating and degrading leukotriene B4, a potent mediator of inflammation. This gene is part of a cluster of cytochrome P450 genes on chromosome 19. Another member of this family, CYP4F8, is approximately 18 kb away. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Apr 2019]