ANO6 (NM_001204803) Human Tagged ORF Clone

CAT#: RG235051

ANO6 (GFP-tagged) - Homo sapiens anoctamin 6 (ANO6), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ANO6
• Synonyms: BDPLT7; SCTS; TMEM16F
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG235051 representing NM_001204803
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAAAGATGAGCAGGAATGTTTTGCTACAAATGGAGGAGGAGGAGGACGACGACGATGGGGATATCG
  GTGATGTTCCTGCCTCCAGGAGGCCTTTTCTGACTCCTCATACTCACCTCCCATCCAGCCTGGTGTTGGA
  AAACCTTGGACAGACAATTGTCCCCGATTTGGGATCACTGGAAAGTCAGCATGATTTTCGAACCCCGGAG
  TTTGAAGAATTTAATGGAAAACCTGACTCCCTCTTTTTTAATGATGGCCAGCGAAGAATTGACTTTGTTC
  TAGTATATGAGGATGAAAGCAGAAAAGAGACCAATAAAAAGGGTACAAATGAAAAACAAAGGAGGAAAAG
  ACAAGCATACGAATCTAACCTTATCTGTCATGGCCTGCAGTTAGAAGCAACAAGATCAGTATTGGATGAC
  AAGCTTGTATTTGTAAAAGTACACGCACCATGGGAGGTGTTATGTACGTATGCTGAGATAATGCACATCA
  AATTGCCTCTGAAACCCAATGATCTGAAAAACCGGTCCTCAGCCTTTGGTACACTCAACTGGTTTACCAA
  AGTCCTCAGTGTAGACGAAAGCATCATCAAGCCAGAGCAAGAGTTTTTCACTGCCCCATTTGAGAAGAAC
  CGGATGAATGATTTTTACATAGTTGATAGAGATGCTTTCTTCAATCCAGCCACCAGAAGCCGCATTGTTT
  ACTTCATCCTCTCTCGGGTCAAGTATCAAGTGATAAACAATGTTAGCAAGTTTGGGATCAACAGACTTGT
  AAACTCTGGGATCTACAAGGCAGCTTTCCCACTCCATGATTGCAAATTCCGCCGTCAGTCAGAGGATCCC
  AGCTGCCCTAATGAACGGTACCTTCTGTACAGAGAATGGGCTCATCCTCGAAGCATATACAAAAAGCAGC
  CCTTGGATCTTATCAGGAAATACTATGGAGAGAAGATTGGAATCTACTTTGCTTGGCTGGGCTATTACAC
  TCAGATGCTTCTCCTGGCCGCAGTTGTAGGAGTGGCTTGCTTTCTCTATGGATATCTTAATCAAGATAAC
  TGTACATGGAGCAAAGAAGTTTGTCATCCTGATATTGGTGGCAAGATCATAATGTGTCCTCAGTGTGATA
  GGCTTTGTCCATTCTGGAAACTCAATATTACTTGCGAGTCCTCAAAGAAATTGTGCATCTTCGACAGTTT
  TGGAACCCTGGTCTTTGCAGTATTTATGGGAGTATGGGTTACCTTGTTTTTGGAGTTTTGGAAGCGACGC
  CAGGCAGAACTTGAGTATGAATGGGATACTGTTGAGTTACAGCAGGAAGAACAAGCCCGACCAGAATACG
  AAGCACGATGTACTCACGTAGTGATAAATGAGATTACTCAGGAAGAAGAACGCATTCCCTTTACTGCCTG
  GGGAAAATGTATACGGATAACCCTCTGTGCCAGTGCTGTCTTTTTCTGGATCCTATTGATCATCGCTTCA
  GTTATTGGGATCATTGTCTATAGGCTCTCGGTGTTCATTGTATTTTCTGCAAAACTTCCCAAGAACATTA
  ATGGAACAGACCCAATCCAGAAATACCTGACTCCACAGACAGCCACGTCCATCACGGCCTCCATCATCAG
  CTTTATAATTATCATGATTCTGAACACCATATATGAAAAAGTGGCAATTATGATTACTAACTTCGAACTC
  CCAAGGACCCAGACTGATTATGAGAACAGCCTCACCATGAAGATGTTCTTATTCCAGTTTGTCAACTACT
  ACTCTTCATGCTTCTACATAGCATTCTTTAAGGGCAAATTTGTAGGCTATCCAGGAGACCCAGTTTATTG
  GTTGGGAAAATACAGAAATGAAGAGTGTGACCCAGGTGGCTGTCTTCTTGAACTGACAACTCAGCTGACA
  ATAATCATGGGAGGAAAAGCAATCTGGAATAACATACAAGAAGTATTATTGCCCTGGATCATGAATCTAA
  TTGGGCGATTTCACAGAGTTTCTGGATCAGAAAAGATAACCCCACGATGGGAACAGGACTACCATCTGCA
  GCCTATGGGCAAACTGGGATTATTTTATGAATATCTTGAAATGATTATTCAGTTTGGGTTCGTCACCTTA
  TTTGTGGCCTCTTTTCCACTGGCCCCTCTGTTGGCTCTCGTGAACAATATATTGGAAATAAGAGTGGACG
  CATGGAAACTGACCACCCAGTTTAGACGCCTGGTACCAGAGAAAGCCCAAGACATTGGAGCATGGCAGCC
  CATCATGCAAGGAATAGCAATTCTGGCTGTGGTGACCAATGCCATGATCATAGCTTTCACGTCGGACATG
  ATCCCCCGCCTAGTGTACTACTGGTCCTTCTCCGTCCCTCCCTACGGGGACCACACTTCCTACACCATGG
  AAGGGTACATCAACAACACTCTCTCCATCTTCAAAGTCGCAGACTTCAAAAACAAAAGCAAGGGAAACCC
  GTACTCTGACCTGGGTAACCATACCACATGCAGGTATCGTGATTTCCGATACCCACCTGGACACCCCCAG
  GAGTATAAACACAACATCTACTATTGGCATGTGATTGCAGCCAAGCTGGCTTTTATCATTGTCATGGAGC
  ACGTCATCTACTCTGTGAAATTTTTCATTTCATATGCAATTCCCGATGTATCAAAACGCACAAAGAGCAA
  GATCCAGAGAGAAAAATACCTAACCCAAAAGCTTCTTCATGAGAATCACCTCAAAGATATGACGAAAAAT
  ATGGGGGTGATAGCTGAGCGGATGATAGAAGCAGTAGATAACAATTTACGGCCAAAATCAGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG235051 representing NM_001204803
  Red=Cloning site Green=Tags(s)
  
  MKKMSRNVLLQMEEEEDDDDGDIGDVPASRRPFLTPHTHLPSSLVLENLGQTIVPDLGSLESQHDFRTPE
  FEEFNGKPDSLFFNDGQRRIDFVLVYEDESRKETNKKGTNEKQRRKRQAYESNLICHGLQLEATRSVLDD
  KLVFVKVHAPWEVLCTYAEIMHIKLPLKPNDLKNRSSAFGTLNWFTKVLSVDESIIKPEQEFFTAPFEKN
  RMNDFYIVDRDAFFNPATRSRIVYFILSRVKYQVINNVSKFGINRLVNSGIYKAAFPLHDCKFRRQSEDP
  SCPNERYLLYREWAHPRSIYKKQPLDLIRKYYGEKIGIYFAWLGYYTQMLLLAAVVGVACFLYGYLNQDN
  CTWSKEVCHPDIGGKIIMCPQCDRLCPFWKLNITCESSKKLCIFDSFGTLVFAVFMGVWVTLFLEFWKRR
  QAELEYEWDTVELQQEEQARPEYEARCTHVVINEITQEEERIPFTAWGKCIRITLCASAVFFWILLIIAS
  VIGIIVYRLSVFIVFSAKLPKNINGTDPIQKYLTPQTATSITASIISFIIIMILNTIYEKVAIMITNFEL
  PRTQTDYENSLTMKMFLFQFVNYYSSCFYIAFFKGKFVGYPGDPVYWLGKYRNEECDPGGCLLELTTQLT
  IIMGGKAIWNNIQEVLLPWIMNLIGRFHRVSGSEKITPRWEQDYHLQPMGKLGLFYEYLEMIIQFGFVTL
  FVASFPLAPLLALVNNILEIRVDAWKLTTQFRRLVPEKAQDIGAWQPIMQGIAILAVVTNAMIIAFTSDM
  IPRLVYYWSFSVPPYGDHTSYTMEGYINNTLSIFKVADFKNKSKGNPYSDLGNHTTCRYRDFRYPPGHPQ
  EYKHNIYYWHVIAAKLAFIIVMEHVIYSVKFFISYAIPDVSKRTKSKIQREKYLTQKLLHENHLKDMTKN
  MGVIAERMIEAVDNNLRPKSE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001204803
• ORF Size: 2793 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001204803.1, NP_001191732.1
• RefSeq Size: 6196
• RefSeq ORF: 2796
• Locus ID: 196527
• Protein Families: Transmembrane
• Gene Summary: This gene encodes a multi-pass transmembrane protein that belongs to the anoctamin family. This protein is an essential component for the calcium-dependent exposure of phosphatidylserine on the cell surface. The scrambling of phospholipid occurs in various biological systems, such as when blood platelets are activated, they expose phosphatidylserine to trigger the clotting system. Mutations in this gene are associated with Scott syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]